Evaluation of human umbilical cord blood as a source of embryonic stem cells

Human umbilical cord blood (HUCB) has been poorly characterised as a source of embryonic stem cells (ESCs). The aim of this study, therefore, was to evaluate HUCB as source of mesenchymal stem cells (MSCs) with embryonic characteristics. HUCB was collected from consenting women undergoing elective caesarean sections. HUCB was meticulously explanted into MesenCult media and incubated. Qualitative and quantitative immunophenotyping of cells was achieved using fluorescein isothiocyanate (FITC) labelled antibodies (CD34, CD45, CD29, CD44, CD73 and CD105) phenotypic markers. Immunocytochemistry was carried out for the human ESC markers CD9, stage-specific embryonic antigen-1 and 4 (SSEA-1 and SSEA-4), E-cadherin, Podocalyxin (PODXL), sex-determining region Y-box 2 (SOX2), NANOG and Octamer (OCT3/4). MSCs were cultured to induce differentiation into adipogenic, osteogenic, chondrogenic and neurogenic cells. Immunocytochemistry was used to identify fatty acid binding protein-4 (FABP-4), osteocalcin, aggrecan, SOX2 and oligodendrocyte-4 (Olig-4) markers. The cells were strongly positive for the MSC markers CD29, CD44, CD73 and CD105; these cells also expressed the ESC markers CD9, SSEA-1 and SSEA-4, E-cadherin, PODXL, SOX2, NANOG and OCT3/4. Additionally, the MSCs expressed the adipogenic FABP-4, osteogenic osteocalcin, chondrogenic aggrecan and neural Olig-4 and SOX2 markers after differentiation. Therefore, HUCB is a rich source for MSCs with embryonic characteristics

Recent Trends in Plasmonic Nanowire Solar Cells

Light trapping is crucial for low-cost and highly efficient nanowire (NW) solar cells (SCs). In order to increase the light absorption through the NWSCs, plasmonic materials can be incorporated inside or above the NW design. In this regard, two novel designs of plasmonic NWSCs are reported and analyzed using 3D finite difference time domain method. The geometrical parameters of the reported designs are studied to improve their electrical and optical efficiencies. The ultimate and power conversion efficiencies (PCE) are used to quantify the conversion efficiency of the light into electricity. The first design relies on funnel shaped SiNWs with plasmonic core while the cylindrical NWs of the second design are decorated by Ag diamond shaped. The calculated ultimate efficiency and PCE of the plasmonic funnel design are equal to 44% and 18.9%, respectively with an enhancement of 43.3 % over its cylindrical NWs counterpart. This enhancement can be explained by the coupling between the three optical modes, supported by the upper cylinder, lower cone and plasmonic material. Moreover, the cylindrical SiNWs decorated by Ag diamond offer an ultimate efficiency and short-circuit current density of 25.7%, and 21.03 mA∕cm2, respectively with an improvement of 63% over the conventional cylindrical SiNWs

Effect of magnesium sulphate on emergence agitation in children undergoing adenotonsillectomy

Background: There is some evidence that intraoperative magnesium infusions prevent postoperative delirium in children.Objective: The aim of the work was to study the effects of intra-operative magnesium sulphate administration on the children's risk of post-adenotonsillectomy post-agitation.Patients and Methods: At Anesthesia & Surgical Intensive Care Department, Zagazig University Hospitals, this randomized, double-blind clinical trial included 90 children with American Society of Anesthesiologists (ASA) physical status classes I or II who were scheduled to undergo adenotonsillectomy. Patients were allocated randomly into two equal groups (45 for each group): Group C: received intraoperative 0.9% normal saline initial loading dose of 0.3ml/kg over 10 min as a bolus, then 0.1ml/kg/hr. of infusion for the length of the procedure. Group MG: Patients were given 30 mg/kg of a 10% solution of magnesium sulphate intraoperatively over the course of 10 minutes as an initial loading dose, then 0.1ml/kg/hr. of infusion for the length of the procedure.Results: Group MG was significantly lower than Group C regarding fentanyl needed and also total dose of fentanyl. As regard Pediatric Anesthesia Emergence Delirium (PAED) score, we found that there was No significant difference regarding pre but at post group MG was significantly lower at post also agitation was significantly associated with Group C.Conclusion: It could be concluded that sevoflurane anesthesia using magnesium sulphate is used for adenotonsillectomy surgery in children. A 30-mg/kg loading dose is given intraoperatively, followed by a continuous infusion at 0.1ml/kg/hr. for the duration of surgery. This combination reduces the incidence of emergent anxiety and the need for postoperative fentanyl

Inappropriate use of antibiotics in the treatment of pharyngo-tonsilitis in children in Khartoum, Sudan

Background: Pharyngo-tonsillitis represents a major public health problem all over the world. Recent studies in Sudan have revealed alarming results reporting antibiotics over prescription in the management of acute tonsillitis.Objectives: The aim of this study was to estimate the prevalence of Group A Beta hemolytic streptococci and document the appropriateness of using antibiotics in the treatment of acute tonsillo-pharyngitis in Jaafar Ibn Auf Pediatrics Hospital.Materials and Methods: A cross sectional hospital based study was conducted in the period January to August 2012 in Jaafar Ibn Auf Hospital, the largest tertiary pediatrics hospital in Sudan. The sample size was 100 including children aged 2-17 years and got antibiotics treatment for their current sore throat. A pharyngeal swab was collected at presentation from tonsils and posterior pharynx. Gram staining was done first, and then Group A Beta hemolytic streptococci were isolated and identified in the laboratory by their growth characteristics. All children included in the study were assessed clinically and subjected to a structural questionnaire. Data were analyzed by SPSS version 19.Results: The estimated prevalence of Group A Beta hemolytic streptococci tonsillitis and/or pharyngitis was 22%, nevertheless the proportion of antibiotic prescription was 100%.Conclusion: Most children were treated inappropriately regarding the need for using antibiotics, the type of antibiotics used and the duration of management. As many studies suggested that increased using of antibiotics may be due to uncertainty of diagnosis, requesting scoring system or rapid diagnostic test can contribute to the reduction of the rate of antibiotics prescription.Keyword: Pharyngo-tonsillitis, inappropriate, children, antibiotics, Suda

Epidemiological study of risk factors in pediatric asthma

Background: Childhood asthma is a major public health problem in Egypt and worldwide. Epidemiologic, physiologic, and social factors appear to be associated with an increased risk of asthma. Objective: The aim of the study was to determine the most frequent risk factors of childhood asthma exacerbation and severity in our community. Methods: This cross sectional study involved 206 asthmatic children, 5 to 15 years old. They were enrolled from the School Students Health Insurance facility of El-Matareya Teaching Hospital and from the Pediatric Outpatient Clinic of Saint Mark Charity Hospital representing several social and residential classes. They were assessed clinically and by peak expiratory flow rate (PEFR). Parents of children were interviewed for symptoms and some demographic, social, environmental, housing and familial data as well as asthma triggers through a comprehensive detailed questionnaire. Results: Residential distribution and social status were significantly associated with asthma severity as most moderate persistent asthmatics lived in semi-urban areas (70.8%) and belonged to the low-level segment of social classification (47.9%). In the majority of the study population (69.9%), a family member or more had a positive history of bronchial asthma, and this was especially evident in moderate persistent cases (70.8%). Passive smoking and dust triggered exacerbations in 48.6% and 65% of the studied sample respectively and in most moderate persistent asthmatics (83.4% and 93.7%). Most houses of moderate asthmatics were infested with cockroaches (91.7%) and domestic animals were present in 56.2%. Recurrent chest infections and cold/flu attacks were strongly associated with asthma exacerbation and severity (93.8% and 93.7% of moderate persistent cases respectively). Most moderate persistent asthmatics (91.7%) reported exercise-induced asthma while 64.6% stated that emotional stress triggered their symptoms. Indoor pollutants such as insecticides, household chemicals and odors were strongly associated with asthma severity and exacerbation especially in moderate persistent cases (triggered symptoms in 66.7%, 52.1% and 58.3% of cases respectively). Conclusion: Smoking, emotional stress and dust were the most significant triggers of asthma exacerbation and severity in our series. Identification and avoidance of risk factors for persistent asthma, combined with early institution of pharmacologic and other intervention strategies, may lead to a better outcome.Keywords: asthma severity; asthma triggers; children; residence; risk factors; smoking; social statusEgypt J Pediatr Allergy Immunol 2007; 5(1): 11-1

Epidemiological study of school performance and asthma medications among asthmatic Egyptian school children

Background: Childhood asthma is a major health problem in Egypt and worldwide. Barriers to reducing the burden of asthma include symptom-based rather than disease-based approaches, tendency of care to be ‘‘acute’’ rather than “regular” and cultural attitudes towards drugs and drug delivery systems. Objective: To measure the disease effect on school performance and to map asthma medications with respect to types, routes and course of use. Methods: This cross sectional study involved 206 Cairene asthmatic school children, enrolled from the school students’ health insurance facility of El-Matareya Teaching Hospital and the pediatric outpatient clinic of Saint Mark Charity Hospital representing different social classes and residential locations. Their ages ranged between 5 and 15 years and they comprised 100 males and 106 females. They were assessed clinically and by peak expiratory flowmetry. Parents or caregivers were interviewed about disease symptoms, school achievement and attendance and medications used including relievers and controllers, route and course of use, whether continuously, intermittently and/or during attacks. Results: Asthma had a strong impact on school achievement and school absence; 77.3% of study population reported school absence due to asthma and 41.3% reported weak to average school achievement. School performance was associated with asthma severity; the majority of moderate asthmatics (64.6%) had average and weak school performance. Number of days of school absence demonstrated highly significant relation with asthma severity; 43.6% of moderate asthmatics had 5-6 absent days/month and 33.4% had 3-4 absent days/month. Combined β2-agonists and xanthines was the most commonly used (54.8%) reliever therapy followed by β2-agonists alone (44.2%) while corticosteroids were the most common controllers used (97.6%). Therapy was taken mainly by oral than inhalation route. Other medications like cromolyns, anti-histamines, anti-leukotrienes and anti-cholinergics were rarely used. Both rectal and injection routes were rarely used also. Conclusion: Asthma has a social burden on asthmatic children as it affects both school achievement and school attendance. Medical management of asthma in Egyptian children still lags behind available medical knowledge. Patients’ and health care givers’ education is a cornerstone in improving the current status of asthma management in Egypt.Keywords: asthma medications; asthma severity; children; school performanceEgypt J Pediatr Allergy Immunol 2009;7(1):23-3

RT-PCR ANALYSIS OF GENES EXPRESSION TO EVALUATE THE BIOMEDICAL IMPORTANCE OF MEDICAL-HERBAL EXTRACTS IN DIABETES TREATMENT

Fenugreek is an annual crop belonging to the legume family grown in most parts of the world. It is known to possess a number of medicinal properties, but a little phytochemical research has been carried out on the seeds of species; Trigonella hamosa Leguminosae. Previously we reported that Trigonella seeds extract are shown to have hypoglycemic effects on experimental diabetic rats. The aim of the current study is to understand the molecular mechanism of antidiabetic actions of Trigonella. Therefore, we examined the expressions levels of genes involved in glucose metabolism in liver of diabetic rats. Upon this basis, streptozotocin (STZ)-diabetic rats are used to assess hepatic glucokinase (GCK), insulin like growth factor-1 (IGF- 1), and glucose transporters (GLUT-2) genes expressions after Trigonella administration by oral intragastric intubation for two months, using RT-PCR assay. The current findings demonstrated that Trigonella reduced significantly glucose level in diabetic rats, while significantly increases insulin serum level. GCK expression levels in hepatic tissue of diabetic-rats are found to be suppressed, while Trigonella treatment induces an increase in hepatic GCK activity. Diabetes due to STZ has a little effect on IGF-1 gene expression, while Trigonella administration elevates mRNA expression significantly. On the other hand, Trigonella reduce and normalize the elevation of GLUT-2 gene expression, which increased due to STZ treatment. These results indicated that Trigonella are powerful antidiabetic agent, induce hypoglycemia by up-regulation of CGK and IGF-1 genes expression and induce an insulin mimetic activity

Parvovirus B19 viremia in children with systemic lupus erythematosus

Background: Parvovirus B19 infection may present with fever, rash, nonerosive arthritis, hepatitis, anemia, thrombocytopenia, leucopenia and positive ANA, B19 infection may be misdiagnosed as new onset systemic lupus erythematosus. At the same time, B19 infection and systemic lupus erythematosus may occur simultaneously in some patients. A casual relationship between B19 infection and classic idiopathic systemic lupus erythematosus has not been demonstrated yet. Objectives: This study was undertaken to investigate the seroprevalence of parvovirus B19 in SLE patients and to search for the different correlates of this viremia with positive results. Methods: This case-control study was conducted on 30 patients with SLE and 30 normal controls. All the children were subjected to detailed medical history, Clinical examination, laboratory estimation as sera from them were examined for parvovirus B19 infection by serological assays using nested polymerase chain reaction and IgG and IgM antiB19 antibodies by ELISA. Results: Parvovirus B19 DNA was detected in 11 of the 30 patients with SLE (33.3 percent) while it was not detected in any of our normal controls. Of the 11 patients with B19 DNA, only two had IgG anti-B19 antibody and one had IgM anti-B19 antibodies, whereas IgG and IgM anti-B19 antibodies were detected in 11(57.8%)and 9 (47.3%)of 19 SLE patients without B19 DNA respectively. B19 DNA was found more commonly in sera from SLE patients without anti-B19 antibodies than in those with anti-B19 antibodies (P < 0.05). Conclusions: parvovirus B19 might induce either idiopathic SLE in a person who is genetically susceptible or it might induce a SLE-like picture. Parvovirus B19 infection in patients with SLE may be due to lack of anti-B19 antibodies because of either the immunocompromised nature of the host or the use of immunosuppressive drugs. There was a higher prevalence of hypocomplementemia in patients with parvovirus B19 viremia than in those without parvovirus. Keywords: Human parvovirus B19, Systemic lupus erythematosus, Nested PCREgypt J Pediatr Allergy Immunol 2011;9(2):71-7