Study of low voltage electroporation technique on hela cell treated with neolamarckia cadamba extracts daing

Electroporation is a process of creating pores on cell by exposing the cell with electric field. This process increases the permeability of the cell. Neolamarckia Cadamba is a natural source that is widely used in the medical arena due to its pharmacological properties. Neolamarckia Cadamba known as kelempayang to Malaysian people. Its mainly planted in the states of Pahang, Perak, Sabah and Sarawak. Electrochemotherapy is a combination of the electroporation process and chemotherapeutic drugs. The Standard electric field parameters for Electrochemotherapy (SEFPECT) are 1000V/cm of pulse amplitude, 100μs of pulse duration, 8 numbers of pulses and 1Hz of frequency. Electrochemotherapy process causes transient burns to patient due to the intensity of pulse amplitude used, which is 1000V/cm. The use of chemotherapeutic various drugs gives side effect to the patient. To overcome the problem, this research suggests to lower the pulse amplitude and longer pulse duration plus combining natural extract of Neolamarckia Cadamba Leaves. In this study, the combination of electroporation process and natural extract is conducted in vitro for 48 hours. The cell used is cervical cancer cell (HeLa cell) and the natural extract from Neolamarckia Cadamba Leaves. The experiment was started to determine the best parameter of pulse amplitude and pulse duration that gives lower viability percentage of HeLa cell. The pulse amplitude started with a 200V/cm, 400V/cm, 600V/cm, 800V/cm and 1000V/cm and the pulse duration at 100μs, 500μs, 1ms, 2ms and 5ms and the result revealed that the best parameter at 600V/cm of pulse amplitude and 5ms of pulse duration. Then the IC50 value of Neolamarckia Cadamba Leaves extract were tested and found to be at 40μg/ml. The findings of these studies have shown positive respond on the combination of these two methods. This was proven by the reduction in the percentage of anti proliferation of HeLa cell where with electroporation 30.08% cell can survive and by combining electroporation and extact of Neolamarckia Cadamba leaves only 2.12% HeLa cell can survive

Combined effect of neolamarckia cadamba leaves and electroporation method on hela cell anti- proliferation process

This study suggests that natural sources may become an important tool in treating cancer. Neolamarckia cadamba (NC) leaves also well-known as “Anthocephalus Cadamba”, is a precious plant in Ayurvedic medicine. HeLa cells are one of the examples of eukaryotic cells type. It is derived from human cervical cancer cells. This experiment is conducted in different concentrations of NC Leaves (1μg/ml, 5μg/ml, 10μg/ml, 20μg/ml, 30μg/ml, 40μg/ml, 50μg/ml, 60μg/ml, 70μg/ml, 80μg/ml, 90μg/ml and 100μg/ml) for 48 hours. This experiment’s result proves that the anti-cancer properties of the extract of NC leaves are by increasing the concentration of extract, the numbers of cell viability will decrease. For contribution, the process of NC leaves extract will be combined with the electroporation process to investigate the effect on HeLa cell. Electroporation parameters used for this study were (voltage 600v/cm, pulse duration 5ms, single pulse)

Study of anti-cancer mechanism: Neolamarckia cadamba leaves exposed with electric field on HeLa cell

-Electroporation occurs when the electric field is applied and cause the permeabilization of the HeLa cell membrane. It has two type that is reversible electroporation and irreversible electroporation. In this study, it suggests that reversible electroporation may become an important technique in treating cancer. In this study cervical cancer cell (HeLa cell) will be used as a sample for electroporation process. After electroporation process completed, compound extract (Neolamarckia Cadamba Leaves) will be used as anti-cancer within the culture media. This study will demonstrate a new method for cancer treatment via combination of natural compound plus the electric field. The treatment aims to investigate and evaluate the electroporation and compound extract test effect on HeLa cell. By combining this two technique electroporation and absorption of the compound extract on HeLa cell potentially might be a new technique for anti-cancer process treatment