IPPH anjur program kesedaran konsep halal kepada 50 kanak-kanak

SERDANG, 19 Dis- Laboratori Perkhidmatan Halal, Institut Penyelidikan Produk Halal (IPPH), Universiti Putra Malaysia (UPM) menganjurkan program PUTRA@Saintis Halal Cilik untuk kanak-kanak bagi menyampaikan pengetahuan asas konsep halal

DNA extraction and species detection in gelatin and gelatin capsule by real-time PCR

The origin of gelatin used in gelatin capsule has always been doubtful as it is not always stated in the labelling. Thus, the halal status of the gelatin is questionable and it becomes a great concern by the Muslims. Gelatin is mainly produced from the skin and bones of pig and cow which undergoes either acid or alkali process. Due to the extreme processing conditions, the DNA may encounter high level of degradation. Further processing of the gelatin into gelatin capsule would make it worst. Hence, the minute amount of DNA contained in the gelain and gelatin capsule require careful extraction method and sensitive detection. In this work, the extraction method is optimized in terms of DNA concentration, DNA fragmentation and the presence of real-time PCR inhibitor. Following quality assessment of the DNA extracted, both bovine and porcine are detected using species-specific real-time PCR systems based on SYBR Green reaction.The quality of the DNA extracted from gelatin and gelatin capsules are low in terms of DNA concentration. Using real-time PCR, up to approximately 300 bp target sequence can be amplified and successful detection of both species has been achieved

Comparison of gene nature used in real-time PCR for porcine identification and quantification: a review

Pork adulteration has been a major concern nowadays for Halal verification. Unintentional pork inclusion by contamination in highly processed food materials involves a minute amount of porcine DNA to be detected, emphasizing the need of specific and sensitive method for porcine detection. Real-time PCR is a widely used technique for species identification that can serve this purpose besides providing a powerful quantification method. Incorporation of a highly sensitive and specific probe can greatly improve the specificity and sensitivity of the assay. However, derivation of PCR primers, either from nuclear DNA (nDNA) or mitochondrial DNA (mtDNA) can relatively affect the sensitivity and specificity of the reaction as well as the quantitative measurement. In this review, both types of DNA are compared in terms of their characteristics and their influence on species identification and quantification using real-time PCR

Hydroxyproline determination for initial detection of halal-critical food ingredients (gelatin and collagen)

Gelatin and collagen are considered halal-critical ingredients as they are typically derived from either bovine or porcine animals. Current analytical methods for determining the sources of gelatin and collagen suffer from limitations in terms of robustness and false positives in peptide matching. Thus, the aim of this study was to investigate the utility of monitoring hydroxyproline, a signature amino acid for gelatin and collagen, for identifying potentially haram foodstuffs. To determine the hydroxyproline profiles among animal- and plant-based samples, one-way univariate analysis of variance followed by pair-wise comparison was used to establish statistical significance. Multivariate chemometric analysis through principal component analysis revealed a discrete distribution pattern among 59 samples due to hydroxyproline variability. Finally, inter- and intra-laboratory comparisons demonstrated the validity and robustness of hydroxyproline determination according to ISO 17025. Thus, this preliminary identification technique will aid the identification of potentially haram foodstuffs