Quantitative Proteomics and Phosphoproteomics Reveal TNF-α-Mediated Protein Functions in Hepatocytes

Increased secretion of proinflammatory cytokines, such as tumor necrosis factor-alpha (TNFα), is often associated with adipose tissue dysregulation, which often accompanies obesity. High levels of TNFα have been linked to the development of insulin resistance in several tissues and organs, including skeletal muscle and the liver. In this study, we examined the complex regulatory roles of TNFα in murine hepatocytes utilizing a combination of global proteomic and phosphoproteomic analyses. Our results show that TNFα promotes extensive changes not only of protein levels, but also the dynamics of their downstream phosphorylation signaling. We provide evidence that TNFα induces DNA replication and promotes G1/S transition through activation of the MAPK pathway. Our data also highlight several other novel proteins, many of which are regulated by phosphorylation and play a role in the progression and development of insulin resistance in hepatocytes

Um Estudo de Usabilidade do Ambiente Cultural Virtual do Museu de Sant'Ana

Este estudo analisa a percepção da usabilidade do ambiente virtual cultural do Museu de Sant’Ana por diferentes tipos de usuários, tais como idosos e jovens. O método utilizado nesta pesquisa propôs três atividades que os voluntários deveriam executar na plataforma do museu virtual. Como resultado, notou-se que conforme a idade se elevava, a quantidade de erros cometidos foi gradativamente aumentando, o que resultou em desempenhos inferiores. Outros fatores como a experiência e o conhecimento de computadores foram refletidos no desempenho ao utilizar o espaço virtual. A satisfação dos usuários ao finalizar a visita foi positiva, em sua grande maioria, mostrando que a interação com o museu virtual foi agradável e proporcionou uma nova possibilidade que muitos usuários não conheciam

Multi-Omics Approach Reveals Dysregulation of Protein Phosphorylation Correlated with Lipid Metabolism in Mouse Non-Alcoholic Fatty Liver

Obesity caused by overnutrition is a major risk factor for non-alcoholic fatty liver disease (NAFLD). Several lipid intermediates such as fatty acids, glycerophospholipids and sphingolipids are implicated in NAFLD, but detailed characterization of lipids and their functional links to proteome and phosphoproteome remain to be elucidated. To characterize this complex molecular relationship, we used a multi-omics approach by conducting comparative proteomic, phoshoproteomic and lipidomic analyses of high fat (HFD) and low fat (LFD) diet fed mice livers. We quantified 2447 proteins and 1339 phosphoproteins containing 1650 class I phosphosites, ofwhich 669 phosphositeswere significantly different between HFD and LFD mice livers. We detected alterations of proteins associated with cellular metabolic processes such as small molecule catabolic process, monocarboxylic acid, long- and medium-chain fatty acid, and ketone body metabolic processes, and peroxisome organization. We observed a significant downregulation of protein phosphorylation in HFD fed mice liver in general. Untargeted lipidomics identified upregulation of triacylglycerols, glycerolipids and ether glycerophosphocholines and downregulation of glycerophospholipids, such as lysoglycerophospholipids, as well as ceramides and acylcarnitines. Analysis of differentially regulated phosphosites revealed phosphorylation dependent deregulation of insulin signaling as well as lipogenic and lipolytic pathways during HFD induced obesity. Thus, this study reveals amolecular connection between decreased protein phosphorylation and lipolysis, as well as lipid-mediated signaling in diet-induced obesity

Quantitative Proteomics and Phosphoproteomics Reveal TNF-α-Mediated Protein Functions in Hepatocytes

Increased secretion of proinflammatory cytokines, such as tumor necrosis factor-alpha (TNFα), is often associated with adipose tissue dysregulation, which often accompanies obesity. High levels of TNFα have been linked to the development of insulin resistance in several tissues and organs, including skeletal muscle and the liver. In this study, we examined the complex regulatory roles of TNFα in murine hepatocytes utilizing a combination of global proteomic and phosphoproteomic analyses. Our results show that TNFα promotes extensive changes not only of protein levels, but also the dynamics of their downstream phosphorylation signaling. We provide evidence that TNFα induces DNA replication and promotes G1/S transition through activation of the MAPK pathway. Our data also highlight several other novel proteins, many of which are regulated by phosphorylation and play a role in the progression and development of insulin resistance in hepatocytes

Um Estudo de Usabilidade do Ambiente Cultural Virtual do Museu de Sant'Ana

Este estudo analisa a percepção da usabilidade do ambiente virtual cultural do Museu de Sant’Ana por diferentes tipos de usuários, tais como idosos e jovens. O método utilizado nesta pesquisa propôs três atividades que os voluntários deveriam executar na plataforma do museu virtual. Como resultado, notou-se que conforme a idade se elevava, a quantidade de erros cometidos foi gradativamente aumentando, o que resultou em desempenhos inferiores. Outros fatores como a experiência e o conhecimento de computadores foram refletidos no desempenho ao utilizar o espaço virtual. A satisfação dos usuários ao finalizar a visita foi positiva, em sua grande maioria, mostrando que a interação com o museu virtual foi agradável e proporcionou uma nova possibilidade que muitos usuários não conheciam

Additional file 2 of Proteomic analysis reveals that aging rabbit vocal folds are more vulnerable to changes caused by systemic dehydration

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Additional file 1 of Proteomic analysis reveals that aging rabbit vocal folds are more vulnerable to changes caused by systemic dehydration

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Additional file 3 of Proteomic analysis reveals that aging rabbit vocal folds are more vulnerable to changes caused by systemic dehydration

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