Pelatihan Penerapan Kebijakan Publik Bagi Aparatur Desa di Desa Tegalarum Kecamatan Mranggen Kabupaten Demak Provinsi Jawa Tengah

Diharapkan melalui kegiatan ini akan mendorong kecakapan aparatur desa dalam pengambilam keputusan publik dengan mengedepankan skala prioritas keputusan yang diambil. Tujuan dari pengabdian masyarakat adalah memberikan pelatihan penerapan pengambilan kebijakan publik dalam meningkatkan kapasitas aparatur pemerintahan desa. Metode kegiatan digunakan Participatory Learning and Action. Kegiatan dilakukan dengan menekankan pada kegiatan ceramah, diskusi, curah pendapat yang dilakukan secara interaktif dengan anggota kelompok dan dilanjutkan dengan aksi atau kegiatan riil yang relevan dengan materi pelatihan. Hasil kegiatan pengabdian masyarakat berjalan sesuai dengan rencana yang telah ditetapkan, peserta memilki respon dan antusias terhadap pelatihan dan tercipta Perubahan pengetahuan, sikap dan keterampilan dalam pengambilan kepusan kebijakan publik

Sensitivity and Specificity of the SelfAdministered HPV Testing in Detecting Precancerous Conditions of the Cervix and Cervical Cancer

Objective: To determine the sensitivity and specificity of selfadministered Human Papilloma virus (HPV) test in detecting pre-cancerous lesions and high degree cervical cancer. Methods: A case control study design was used in this study. This study was conducted at Dr. Wahidin Sudirohusodo Hospital and its affiliate hospitals during the period of October 2014 to May 2015. Measurement data using HPV self test grant from the Dutch School Netherlands, Evalyn Brush. Collecting data used questionnaires before and after doing HPV self test. Laboratory tests are carried out in Kalbe Genomic using HPV Genotyping capable detect 35 types of the HPV virus. Results: A total of 101 subjects were involved in this study. The subjects were divided into two groups, the case and the controls (n = 50/51). Level of sensitivity and specificity were obtained for HPV self test (56% vs 98%). The level of acceptance of new tests by 62.37% (63 out of 101 respondents) of them admitted that the new test is simple / easy enough to do. The acuracy rate of diagnostic test of this examination is 79%. Conclusion: Self-administered HPV test could be used as an alternative or primary screening for cervical cancer. [Indones J Obstet Gynecol 2017; 5-3: 173-179] Keywords: HPV, self, specificity, the sensitivit

Sensitivity and Specificity of the SelfAdministered HPV Testing in Detecting Precancerous Conditions of the Cervix and Cervical Cancer: sensitivitas dan Spesifisitas Pemeriksaan Mandiri dalam Mendeteksi Lesi Prakanker dan Kanker Serviks

Objective: To determine the sensitivity and specificity of selfadministered Human Papilloma virus (HPV) test in detecting pre-cancerous lesions and high degree cervical cancer. Methods: A case control study design was used in this study. This study was conducted at Dr. Wahidin Sudirohusodo Hospital and its affiliate hospitals during the period of October 2014 to May 2015. Measurement data using HPV self test grant from the Dutch School Netherlands, Evalyn Brush. Collecting data used questionnaires before and after doing HPV self test. Laboratory tests are carried out in Kalbe Genomic using HPV Genotyping capable detect 35 types of the HPV virus. Results: A total of 101 subjects were involved in this study. The subjects were divided into two groups, the case and the controls (n = 50/51). Level of sensitivity and specificity were obtained for HPV self test (56% vs 98%). The level of acceptance of new tests by 62.37% (63 out of 101 respondents) of them admitted that the new test is simple / easy enough to do. The acuracy rate of diagnostic test of this examination is 79%. Conclusion: Self-administered HPV test could be used as an alternative or primary screening for cervical cancer. [Indones J Obstet Gynecol 2017; 5-3: 173-179] Keywords: HPV, self, specificity, the sensitivit

The Effect of Visible Light Cure (VLC) Exposure to Gingival Tissue\u27s Sprague Dawley Rats

Visible Light Cure (VLC) is a blue light used in dentistry as an activator for restorative material and fixed orthodontic bonding. The wavelength of VLC is between 400-500 nm and considered non-ionizing radiation that can produce free radicals. According to previous research, the light at wavelength < 500 nm could inhibit cells mitosis, cause cells damage, and reduce cells growth and inflammation. The purpose of this study was to investigate the effect of VLC exposure on gingival epithelial thickness, total neutrophil and macrophage count of gingival connective tissue of Sprague dawley rats. The subjects of this study consisted of 20 Sprague dawley rats, in 2-3 months of age and divided into 4 groups. Each group was 5 rats. The rats in each group were sacrificed before (0 day, as group A) and after 1st (group B), 3rd (group C), 5th (group D) day of VLC exposure, respectively. The exposure of VLC was done in labial aspect of cervical anterior teeth of mandible. The distance of exposure was as thick as 2 layers of celluloid strip and the histological specimens were stained by Hematoxylin Eosin. Each specimen was measured for its gingival epithelial thickness by using a micrometer and the number of neutrophil and macrophage were counted. The data of gingival epithelial thickness from 4 groups were analyzed by Kruskal Wallis. The number of neutrophil and macrophage were analyzed by u sing one way ANOVA. The results of this study showed that there were significant differences among groups on the thickness of gingival epithelial, the number of neutrophil and macrophage in the gingival connective tissue of Sprague dawley rats. The result of this study indicated that VLC exposure might decrease the thickness of gingival epithelial but increase the number of neutrophil and macrophage of gingival connective tissue of Sprague dawley rats

Difference in expression between AQP1 and AQP5 in porcine endometrium and myometrium in response to steroid hormones, oxytocin, arachidonic acid, forskolin and cAMP during the mid-luteal phase of the estrous cycle and luteolysis

BACKGROUND: Recently, we demonstrated in vitro that AQP1 and AQP5 in the porcine uterus are regulated by steroid hormones (P4, E2), arachidonic acid (AA), forskolin (FSK) and cAMP during the estrous cycle. However, the potential of the porcine separated uterine tissues, the endometrium and myometrium, to express these AQPs remains unknown. Thus, in this study, the responses of AQP1 and AQP5 to P4, E2 oxytocin (OT), AA, FSK and cAMP in the porcine endometrium and myometrium were examined during the mid-luteal phase of the estrous cycle and luteolysis.METHODS: Real-time PCR and western blot analysis.RESULTS: Progesterone up-regulated the expression of AQP1/AQP5 mRNAs and proteins in the endometrium and myometrium, especially during luteolysis. Similarly, E2 also stimulated the expression of both AQPs, but only in the endometrium. AA led to the upregulation of AQP1/AQP5 in the endometrium during luteolysis. In turn, OT increased the expression of AQP1/AQP5 mRNAs and proteins in the myometrium during mid-luteal phase. Moreover, a stimulatory effect of forskolin and cAMP on the expression of AQP1/AQP5 mRNAs and proteins in the endometrium and myometrium dominated during luteolysis, but during the mid-luteal phase their influence on the expression of these AQPs was differentiated depending on the type of tissue and the incubation duration.CONCLUSIONS: These results seem to indicate that uterine tissues; endometrium and myometrium, exhibit their own AQP expression profiles in response to examined factors. Moreover, the responses of AQP1/AQP5 at mRNA and protein levels to the studied factors in the endometrium and myometrium are more pronounced during luteolysis. This suggests that the above effects of the studied factors are connected with morphological and physiological changes taking place in the pig uterus during the estrous cycle.</p

Prostaglandin F2-alpha receptor (FPr) expression on porcine corpus luteum microvascular endothelial cells (pCL-MVECs)

<p>Abstract</p> <p>Background</p> <p>The corpus luteum (CL) is a transient endocrine gland and prostaglandin F2-alpha is considered to be the principal luteolysin in pigs. In this species, the in vivo administration of prostaglandin F2-alpha induces apoptosis in large vessels as early as 6 hours after administration. The presence of the prostaglandin F2-alpha receptor (FPr) on the microvascular endothelial cells (pCL-MVECs) of the porcine corpus luteum has not yet been defined. The aim of the study was to assess FPr expression in pCL-MVECs in the early and mid-luteal phases (EL-p, ML-p), and during pregnancy (P-p). Moreover, the effectiveness of prostaglandin F2-alpha treatment in inducing pCL-MVEC apoptosis was tested.</p> <p>Methods</p> <p>Porcine CLs were collected in the EL and ML phases and during P-p. All CLs from each animal were minced together and the homogenates underwent enzymatic digestion. The pCL-MVECs were then positively selected by an immunomagnetic separation protocol using Dynabeads coated with anti-CD31 monoclonal antibody and seeded in flasks in the presence of EGM 2-MV (Microvascular Endothelial Cell Medium-2). After 4 days of culture, the cells underwent additional immunomagnetic selection and were seeded in flasks until the confluent stage.</p> <p>PCR Real time, western blot and immunodetection assays were utilized to assess the presence of FPr on pCL-MVEC primary cultures. Furthermore, the influence of culture time (freshly isolated, cultured overnight and at confluence) and hormonal treatment (P4 and E2) on FPr expression in pCL-MVECs was also investigated. Apoptosis was detected by TUNEL assay of pCL-MVECs exposed to prostaglandin F2-alpha.</p> <p>Results</p> <p>We obtained primary cultures of pCL-MVECs from all animals. FPr mRNA and protein levels showed the highest value (ANOVA) in CL-MVECs derived from the early-luteal phase. Moreover, freshly isolated MVECs showed a higher FPr mRNA value than those cultured overnight and confluent cells (ANOVA). prostaglandin F2-alpha treatment failed to induce an apoptotic response in all the pCL-MVEC cultures.</p> <p>Conclusion</p> <p>Our data showing the presence of FPr on MVECs and the inability of prostaglandin F2-alpha to evoke an in vitro apoptotic response suggest that other molecules or mechanisms must be considered in order to explain the in vivo direct pro-apoptotic effect of prostaglandin F2-alpha at the endothelial level.</p

Peranan ulama dalam pembangunan sosial budaya masyarakat jawa tengah

vi+102hlm.;21c

I Wayan Beratha : karya dan pengabdiannya

Buku ini menjelaskan tentang biografi, mencakup karya dan pengabdian dari seorang I Wayan Beratha.viii, 96 hlm.; 21 cm