Diabrotica virgifera virgifera LeConte in confrontation mood : simultaneous geographical and host spectrum expansion in southeastern Slovenia

Diabrotica virgifera virgifera LeConte, in its original North American habitat also known as western corn rootworm beetle, actively continues its expansion to new territories and uses Homo sapiens as its prime vector. It took only 15 years to spread to and occupy the southeastern and central parts of Europe, so far with the exception of Denmark where it has not been documented as of 2007. Economic thresholds have been reached and surpassed only in Southeast European countries like Slovakia, Hungary, Serbia, Eastern Croatia, Romania and Northern Italy. But both, the area affected and the severity of symptoms are increasing. Model calculations by a number of authors (Baufeld & Enzian, 2005 a and b; Hongmei Li & al. 2006, CLIMEX model) indicate a definitive propensity of D. v. virgifera to expand its currently occupied territory to regions with moderate temperatures and Zea mays cultivation. East Africa and Eastern Asia are included in the list of potential candidates for future inadvertent introduction. In most discussions it is tacitly and erroneously assumed that Z. mays is the only or the only important host of D. v. virgifera. Our recent observations in Eastern Slovenia on the oil pumpkin Cucurbita pepo indicate, however, that this simplifying assumption is notlonger strictly valid. It has to be modified in light of new evidence. Here, we report a few field experiments conducted in August of 2006 clarifying the host status of C. pepo in a European country.Diabrotica virgifera virgifera LeConte (Coleoptera: Chysomelidae), im deutschsprachigen Raum als Westlicher Maiswurzelbohrer bekannt, ist ein von der Neuen Welt nach Europa eingeschleppter Schädling. Er gelangte in mindestens drei Einwanderungsschüben, die durch molekulargenetische Untersuchungen unterscheidbar sind, nach Europa. Innerhalb der letzten eineinhalb Jahrzehnte wurde, mit Ausnahme von Dänemark, die gesamte Fläche Südost- und Zentraleuropas vom Käfer besiedelt. In mehreren osteuropäischen Ländern ist die ökonomische Schadensschwelle bereits überschritten. Bis 2006 galt Zea mays als einzige bekannte europäische Wirtspflanze. Allerdings deuten neueste Beobachtungen in Ostslowenien vom August 2006 auf kleine Zahlen von Käfern am Ölkürbis Cucurbita pepo und damit auf eine Ausdehnung des Wirtsspektrums von D. v. virgifera hin. Der Käfer tritt in kleinen Zahlen als Besucher von Ölkürbisblüten mit einer Häufigkeit von 0,1 % auf. Er ist aber auch in geringer Häufigkeit in Kairomon- und Pheromonfallen in Ölkürbisfeldern südlich von Gaberje in Ostslowenien anzutreffen. Dieses Ergebnis stellt den zukünftigen Wert des Fruchtwechsels in Frage, der bisher als eine der wirksamsten und nachhaltigsten Methoden des integrierten Pflanzenschutzes galt

Plasma damage control : from biomolecules to cells and skin

The antibacterial and cell-proliferative character of atmospheric pressure plasma jets (APPJs) helps in the healing process of chronic wounds. However, control of the plasma-biological target interface remains an open issue. High vacuum ultraviolet/ultraviolet (VUV/UV) radiation and RONS flux from plasma may cause damage of a treated tissue; therefore, controlled interaction is essential. VUV/UV emission from argon APPJs and radiation control with aerosol injection in plasma effluent is the focus of this research. The aerosol effect on radiation is studied by a fluorescent target capable of resolving the plasma oxidation footprint. In addition, DNA damage is evaluated by plasmid DNA radiation assay and cell proliferation assay to assess safety aspects of the plasma jet, the effect of VUV/UV radiation, and its control with aerosol injection. Inevitable emission of VUV/UV radiation from plasmas during treatment is demonstrated in this work. Plasma has no antiproliferative effect on fibroblasts in short treatments (t < 60 s), while long exposure has a cytotoxic effect, resulting in decreased cell survival. Radiation has no effect on cell survival in the medium due to absorption. However, a strong cytotoxic effect on the attached fibroblasts without the medium is apparent. VUV/UV radiation contributes 70% of the integral plasma effect in induction of single- and double-strand DNA breaks and cytotoxicity of the attached cells without the medium. Survival of the attached cells increases by 10% when aerosol is introduced between plasma and the cells. Injection of aerosol in the plasma effluent can help to control the plasma-cell/tissue interaction. Aerosol droplets in the effluent partially absorb UV emission from the plasma, limiting photon flux in the direction of the biological target. Herein, cold and safe plasma-aerosol treatment and a safe operational mode of treatment are demonstrated in a murine model

Comparison of cauliflower-insect-fungus interactions and pesticides for cabbage root fly control

International audienceCabbage root fly (Delia radicum L.) control represents a major challenge in brassica production, therefore different management strategies for its control were tested in conventionally managed open field cauliflower production. Strategies included treatments with low-risk methods such as nitrogen lime, the insecticide spinosad and the Beauveria bassiana ATCC 74040-based biopesticide Naturalis. Their effects were compared with treatments based on nonformulated fungal species Metarhizium brunneum, B. bassiana, Clonostachys solani, Trichoderma atroviride, T. koningiopsis, and T. gamsii and commercial insecticides.-cyhalothrin and thiamethoxam. Spinosad and thiamethoxam were pipetted to individual plants before transplanting; lambda-cyhalothrin was sprayed after transplanting; nitrogen lime was applied at first hoeing. Nonformulated fungi were delivered onto cauliflower plantlets' roots as a single pretransplantation inoculation. The cabbage root fly population dynamics exhibited a strong spatiotemporal variation. The lowest number of cabbage root fly pupae recovered from cauliflower roots in the field experiments was recorded in plants treated with spinosad (significant reduction), followed by Naturalis and one of the tested M. brunneum strains (nonsignificant reduction). Significantly more pupae were counted in the nitrogen lime treatment. The field experiments showed that a single drench of cauliflower plantlets with spinosad offered consistent and enduring cabbage root fly control. Naturalis and nonformulated fungal isolates did not decrease cabbage root fly pressure significantly, apparently due to lack of statistical power. The implications of the substantial intra-and inter-annual pest pressure variation and the benefits of using single plant treatments are discussed, and recommendations for improvement of rhizosphere-competence utilizing biological control strategies provided

Transmission and Epidemiology of Potato virus Y

International audienceAs obligate parasites, plant viruses, require in order to survive, to be transmitted to another plant. Experimentally, viruses such as Potato virus Y (PVY) can be transmitted by mechanical means such as wounding and grafting. In its natural environment, PVY transmission is mediated by sap-feeding aphid vector, or vegetatively through propagated organs such as potato tubers. A vast number of aphid species have been reported to transmit PVY in a non-persistent manner with variable efficiency to a large number of solanaceous and non-solanaceous plant species including weeds and ornamentals. Several sensory stimuli will influence host selection and feeding behaviour of the aphid and will strongly influence virus epidemiology. The interactions between the virus, its vector, and the environment are complex and are the focus of many studies aiming to understand the molecular basis of these interactions and their impact on disease development. This chapter will present the current knowledge of PVY transmission, epidemiology, and its management in different countries