Thermal/structural analyses of several hydrogen-cooled leading-edge concepts for hypersonic flight vehicles

The aerodynamic heating at high flight Mach numbers, when shock interference heating is included, can be extremely high and can exceed the capability of most conventional metallic and potential ceramic materials available. Numerical analyses of the heat transfer and thermal stresses are performed on three actively cooled leading-edge geometries (models) made of three different materials to address the issue of survivability in a hostile environment. These analyses show a mixture of results from one configuration to the next. Results for each configuration are presented and discussed. Combinations of enhanced internal film coefficients and high material thermal conductivity of copper and tungsten are predicted to maintain the maximum wall temperature for each concept within acceptable operating limits. The exception is the TD nickel material which is predicted to melt for most cases. The wide range of internal impingement film coefficients (based on correlations) for these conditions can lead to a significant uncertainty in expected leading-edge wall temperatures. The equivalent plastic strain, inherent in each configuration which results from the high thermal gradients, indicates a need for further cyclic analysis to determine component life

A glimpse into the future of genome-enabled plant biology from the shores of Cold Spring Harbor

A report on the 10(th) plant genome meeting entitled ‘Plant genomes and biotechnology: from genes to networks’, held at Cold Spring Harbor Laboratory, 2–5 December, 2015

Effects of Altitude on Step Test Performance

Please view abstract in the attached PDF file

Design of a high-temperature experiment for evaluating advanced structural materials

This report describes the design of an experiment for evaluating monolithic and composite material specimens in a high-temperature environment and subject to big thermal gradients. The material specimens will be exposed to aerothermal loads that correspond to thermally similar engine operating conditions. Materials evaluated in this study were monolithic nickel alloys and silicon carbide. In addition, composites such as tungsten/copper were evaluated. A facility to provide the test environment has been assembled in the Engine Research Building at the Lewis Research Center. The test section of the facility will permit both regular and Schlieren photography, thermal imaging, and laser Doppler anemometry. The test environment will be products of hydrogen-air combustion at temperatures from about 1200 F to as high as 4000 F. The test chamber pressure will vary up to 60 psia, and the free-stream flow velocity can reach Mach 0.9. The data collected will be used to validate thermal and stress analysis models of the specimen. This process of modeling, testing, and validation is expected to yield enhancements to existing analysis tools and techniques

Evidence of a Massive Stellar Disruption in the X-ray Spectrum of ASASSN-14li

The proximity and duration of the tidal disruption event (TDE) ASASSN-14li led to the discovery of narrow, blue-shifted absorption lines in X-rays and UV. The gas seen in X-ray absorption is consistent with bound material close to the apocenter of elliptical orbital paths, or with a disk wind similar to those seen in Seyfert-1 active galactic nuclei. We present a new analysis of the deepest high-resolution XMM-Newton and Chandra spectra of ASASSN-14li. Driven by the relative strengths of He-like and H-like charge states, the data require [N/C] > 2.4, in qualitative agreement with UV spectral results. Flows of the kind seen in the X-ray spectrum of ASASSN-14li were not clearly predicted in simulations of TDEs; this left open the possibility that the observed absorption might be tied to gas released in prior AGN activity. However, the abundance pattern revealed in this analysis points to a single star rather than a standard AGN accretion flow comprised of myriad gas contributions. The simplest explanation of the data is likely that a moderately massive star (M ~ 3 Msun) with significant CNO processing was disrupted. An alternative explanation is that a lower mass star was disrupted that had previously been stripped of its envelope. We discuss the strengths and limitations of our analysis and these interpretations.Comment: Accepted for publication in ApJ Letter

Rapid Synthesis of a Long Double-Stranded Oligonucleotide from a Single-Stranded Nucleotide Using Magnetic Beads and an Oligo Library

Chemical synthesis of oligonucleotides is a widely used tool in the field of biochemistry. Several methods for gene synthesis have been introduced in the growing area of genomics. In this paper, a novel method of constructing dsDNA is proposed. Short (28-mer) oligo fragments from a library were assembled through successive annealing and ligation processes, followed by PCR. First, two oligo fragments annealed to form a dsDNA molecule. The double- stranded oligo was immobilized onto magnetic beads (solid support) via streptavidinbiotin binding. Next, single-stranded oligo fragments were added successively through ligation to form the complete DNA molecule. The synthesized DNA was amplified through PCR and gel electrophoresis was used to characterize the product. Sanger sequencing showed that more than 97% of the nucleotides matched the expected sequence. Extending the length of the DNA molecule by adding single-stranded oligonucleotides from a basis set (library) via ligation enables a more convenient and rapid mechanism for the design and synthesis of oligonucleotides on the go. Coupled with an automated dispensing system and libraries of short oligo fragments, this novel DNA synthesis method would offer an efficient and cost-effective method for producing dsDNA

Data-Driven Artificial Intelligence for Calibration of Hyperspectral Big Data

Near-earth hyperspectral big data present both huge opportunities and challenges for spurring developments in agriculture and high-throughput plant phenotyping and breeding. In this article, we present data-driven approaches to address the calibration challenges for utilizing near-earth hyperspectral data for agriculture. A data-driven, fully automated calibration workflow that includes a suite of robust algorithms for radiometric calibration, bidirectional reflectance distribution function (BRDF) correction and reflectance normalization, soil and shadow masking, and image quality assessments was developed. An empirical method that utilizes predetermined models between camera photon counts (digital numbers) and downwelling irradiance measurements for each spectral band was established to perform radiometric calibration. A kernel-driven semiempirical BRDF correction method based on the Ross Thick-Li Sparse (RTLS) model was used to normalize the data for both changes in solar elevation and sensor view angle differences attributed to pixel location within the field of view. Following rigorous radiometric and BRDF corrections, novel rule-based methods were developed to conduct automatic soil removal; and a newly proposed approach was used for image quality assessment; additionally, shadow masking and plot-level feature extraction were carried out. Our results show that the automated calibration, processing, storage, and analysis pipeline developed in this work can effectively handle massive amounts of hyperspectral data and address the urgent challenges related to the production of sustainable bioenergy and food crops, targeting methods to accelerate plant breeding for improving yield and biomass traits

Conserved Daily Transcriptional Programs in Carica papaya

Most organisms have internal circadian clocks that mediate responses to daily environmental changes in order to synchronize biological functions to the correct times of the day. Previous studies have focused on plants found in temperate and sub-tropical climates, and little is known about the circadian transcriptional networks of plants that typically grow under conditions with relatively constant day lengths and temperatures over the year. In this study we conducted a genomic and computational analysis of the circadian biology of Carica papaya, a tropical tree. We found that predicted papaya circadian clock genes cycle with the same phase as Arabidopsis genes. The patterns of time-of-day overrepresentation of circadian-associated promoter elements were nearly identical across papaya, Arabidopsis, rice, and poplar. Evolution of promoter structure predicts the observed morning- and evening-specific expression profiles of the papaya PRR5 paralogs. The strong conservation of previously identified circadian transcriptional networks in papaya, despite its tropical habitat and distinct life-style, suggest that circadian timing has played a major role in the evolution of plant genomes, consistent with the selective pressure of anticipating daily environmental changes. Further studies could exploit this conservation to elucidate general design principles that will facilitate engineering plant growth pathways for specific environments

Global Profiling of Rice and Poplar Transcriptomes Highlights Key Conserved Circadian-Controlled Pathways and cis-Regulatory Modules

Circadian clocks provide an adaptive advantage through anticipation of daily and seasonal environmental changes. In plants, the central clock oscillator is regulated by several interlocking feedback loops. It was shown that a substantial proportion of the Arabidopsis genome cycles with phases of peak expression covering the entire day. Synchronized transcriptome cycling is driven through an extensive network of diurnal and clock-regulated transcription factors and their target cis-regulatory elements. Study of the cycling transcriptome in other plant species could thus help elucidate the similarities and differences and identify hubs of regulation common to monocot and dicot plants.Using a combination of oligonucleotide microarrays and data mining pipelines, we examined daily rhythms in gene expression in one monocotyledonous and one dicotyledonous plant, rice and poplar, respectively. Cycling transcriptomes were interrogated under different diurnal (driven) and circadian (free running) light and temperature conditions. Collectively, photocycles and thermocycles regulated about 60% of the expressed nuclear genes in rice and poplar. Depending on the condition tested, up to one third of oscillating Arabidopsis-poplar-rice orthologs were phased within three hours of each other suggesting a high degree of conservation in terms of rhythmic gene expression. We identified clusters of rhythmically co-expressed genes and searched their promoter sequences to identify phase-specific cis-elements, including elements that were conserved in the promoters of Arabidopsis, poplar, and rice.Our results show that the cycling patterns of many circadian clock genes are highly conserved across poplar, rice, and Arabidopsis. The expression of many orthologous genes in key metabolic and regulatory pathways is diurnal and/or circadian regulated and phased to similar times of day. Our results confirm previous findings in Arabidopsis of three major classes of cis-regulatory modules within the plant circadian network: the morning (ME, GBOX), evening (EE, GATA), and midnight (PBX/TBX/SBX) modules. Identification of identical overrepresented motifs in the promoters of cycling genes from different species suggests that the core diurnal/circadian cis-regulatory network is deeply conserved between mono- and dicotyledonous species