230 research outputs found
Sequential occurrence of thrombotic thrombocytopenic purpura, essential thrombocythemia, and idiopathic thrombocytopenic purpura in a 42-year-old African-American woman: a case report and review of the literature
<p>Abstract</p> <p>Introduction</p> <p>Thrombotic thrombocytopenic purpura and idiopathic thrombocytopenic purpura are two well recognized syndromes that are characterized by low platelet counts. In contrast, essential thrombocythemia is a myeloproliferative disease characterized by abnormally high platelet numbers.</p> <p>The coexistence of thrombotic thrombocytopenic purpura and idiopathic thrombocytopenic purpura in a single patient has been reported in the literature on a few occasions. However, having essential thrombocythemia complicating the picture has never been reported before.</p> <p>Case presentation</p> <p>We present a case where thrombotic thrombocytopenic purpura, essential thrombocythemia, and idiopathic thrombocytopenic purpura were diagnosed in a 42-year-old African-American woman in the space of a few years; we are reporting this case with the aim of drawing attention to this undocumented occurrence, which remains under investigation.</p> <p>Conclusions</p> <p>As the three conditions have different natural histories and require different treatment modalities, it is important to recognize that these diseases may be seen sequentially. This case emphasizes the importance of reviewing peripheral blood smears for evaluation of thrombocytopenia and bone marrow aspirations for diagnosis of thrombocythemia in order to reach an accurate diagnosis and tailor therapy accordingly. Moreover, this case demonstrates the variability and complexity of platelet disorders. This occurrence of three different types of platelet disorders in one patient remains a pure observation on our part; regardless, this does raise the possibility of a common underlying, as yet undiscovered, pathophysiology that could explain the phenomenon.</p
Conversion of the Mycotoxin Patulin to the Less Toxic Desoxypatulinic Acid by the Biocontrol Yeast Rhodosporidium kratochvilovae Strain LS11
Se describe en este artículo el descubrimiento de la degradación de la micotoxina patulina por una levaduraThe infection of stored apples by the fungus Penicillium expansum causes the contamination of fruits and fruit-derived
products with the mycotoxin patulin, which is a major issue in food safety. Fungal attack can be prevented by beneficial
microorganisms, so-called biocontrol agents. Previous time-course thin layer chromatography analyses showed that the aerobic
incubation of patulin with the biocontrol yeast Rhodosporidium kratochvilovae strain LS11 leads to the disappearance of the
mycotoxin spot and the parallel emergence of two new spots, one of which disappears over time. In this work, we analyzed the
biodegradation of patulin effected by LS11 through HPLC. The more stable of the two compounds was purified and characterized by
nuclear magnetic resonance as desoxypatulinic acid, whose formation was also quantitated in patulin degradation experiments. After
R. kratochvilovae LS11 had been incubated in the presence of 13C-labeled patulin, label was traced to desoxypatulinic acid, thus
proving that this compound derives from the metabolization of patulin by the yeast. Desoxypatulinic acid was much less toxic than
patulin to human lymphocytes and, in contrast to patulin, did not react in vitro with the thiol-bearing tripeptide glutathione. The
lower toxicity of desoxypatulinic acid is proposed to be a consequence of the hydrolysis of the lactone ring and the loss of functional
groups that react with thiol groups. The formation of desoxypatulinic acid from patulin represents a novel biodegradation pathway
that is also a detoxification process
Further characterization of ADAMTS-13 inactivation by thrombin
Background: The multimeric size and platelet-tethering function of von Willebrand factor (VWF) are modulated by the plasma metalloprotease, a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS-13). In vitro ADAMTS-13 is susceptible to proteolytic inactivation by thrombin. Objectives: In this study, we aimed to characterize the inactivation of ADAMTS-13 by thrombin and to assess its physiological significance. Methods and results: By N-terminal sequencing of cleavage products, and by mutagenesis, we identified the principal thrombin cleavage sites in ADAMTS-13 as R257 and R1176. Using a library of 76 thrombin mutants, we highlighted the functional importance of exosite I on thrombin in the proteolysis of ADAMTS-13. Proteolysis of ADAMTS-13 by thrombin caused an 8-fold reduction in its affinity for VWF that contributed to its loss of VWF-cleaving function. Intriguingly, thrombin-cleaved ADAMTS-13 both bound and proteolyzed a short recombinant VWF A2 domain substrate (VWF115) normally. Following activation of coagulation in normal plasma, endogenous ADAMTS-13, but not added ADAMTS-13, appeared resistant to coagulation-induced fragmentation. An estimation of the Km for ADAMTS-13 proteolysis by thrombin was appreciably higher than the physiological concentration of ADAMTS-13. This was corroborated by the comparatively low affinity of ADAMTS-13 for thrombin (KD 95 nm). Conclusions: Together, our data suggest that ADAMTS-13 is protected from rapid proteolytic inactivation by thrombin in normal plasma. Whether this remains the case under pathological situations involving elevated/sustained generation of thrombin remains unclear
Mechanical Activation of a Multimeric Adhesive Protein Through Domain Conformational Change
The mechanical force-induced activation of the adhesive protein von Willebrand factor (VWF), which
experiences high hydrodynamic forces, is essential in initiating platelet adhesion. The importance of the
mechanical force-induced functional change is manifested in the multimeric VWF's crucial role in blood
coagulation, when high fluid shear stress activates plasma VWF (PVWF) multimers to bind platelets.
Here, we showed that a pathological level of high shear stress exposure of PVWF multimers results in
domain conformational changes, and the subsequent shifts in the unfolding force allow us to use force as
a marker to track the dynamic states of the multimeric VWF. We found that shear-activated PVWF
multimers are more resistant to mechanical unfolding than nonsheared PVWF multimers, as indicated in
the higher peak unfolding force. These results provide insight into the mechanism of shear-induced
activation of PVWF multimers
ADAMTS13 phenotype in plasma from normal individuals and patients with thrombotic thrombocytopenic purpura
The activity of ADAMTS13, the von Willebrand factor cleaving protease, is deficient in patients with thrombotic thrombocytopenic purpura (TTP). In the present study, the phenotype of ADAMTS13 in TTP and in normal plasma was demonstrated by immunoblotting. Normal plasma (n = 20) revealed a single band at 190 kD under reducing conditions using a polyclonal antibody, and a single band at 150 kD under non-reducing conditions using a monoclonal antibody. ADAMTS13 was not detected in the plasma from patients with congenital TTP (n = 5) by either antibody, whereas patients with acquired TTP (n = 2) presented the normal phenotype. Following immunoadsorption of immunoglobulins, the ADAMTS13 band was removed from the plasma of the patients with acquired TTP, but not from that of normal individuals. This indicates that ADAMTS13 is complexed with immunoglobulin in these patients. The lack of ADAMTS13 expression in the plasma from patients with hereditary TTP may indicate defective synthesis, impaired cellular secretion, or enhanced degradation in the circulation. This study differentiated between normal and TTP plasma, as well as between congenital and acquired TTP. This method may, therefore, be used as a complement in the diagnosis of TTP
Two rapid assays for screening of patulin biodegradation
Artículo sobre distintos ensayos para comprobar la biodegradación de la patulinaThe mycotoxin patulin is produced by the blue
mould pathogen Penicillium expansum in rotting apples
during postharvest storage. Patulin is toxic to a wide range
of organisms, including humans, animals, fungi and bacteria.
Wash water from apple packing and processing
houses often harbours patulin and fungal spores, which can
contaminate the environment. Ubiquitous epiphytic yeasts,
such as Rhodosporidium kratochvilovae strain LS11 which
is a biocontrol agent of P. expansum in apples, have the
capacity to resist the toxicity of patulin and to biodegrade
it. Two non-toxic products are formed. One is desoxypatulinic
acid. The aim of the work was to develop rapid,
high-throughput bioassays for monitoring patulin degradation
in multiple samples. Escherichia coli was highly
sensitive to patulin, but insensitive to desoxypatulinic acid.
This was utilized to develop a detection test for patulin,
replacing time-consuming thin layer chromatography or
high-performance liquid chromatography. Two assays for patulin degradation were developed, one in liquid medium
and the other in semi-solid medium. Both assays allow the
contemporary screening of a large number of samples. The
liquid medium assay utilizes 96-well microtiter plates and
was optimized for using a minimum of patulin. The semisolid
medium assay has the added advantage of slowing
down the biodegradation, which allows the study and isolation
of transient degradation products. The two assays are
complementary and have several areas of utilization, from
screening a bank of microorganisms for biodegradation
ability to the study of biodegradation pathways
Halo Excitation of He in Inelastic and Charge-Exchange Reactions
Four-body distorted wave theory appropriate for nucleon-nucleus reactions
leading to 3-body continuum excitations of two-neutron Borromean halo nuclei is
developed. The peculiarities of the halo bound state and 3-body continuum are
fully taken into account by using the method of hyperspherical harmonics. The
procedure is applied for A=6 test-bench nuclei; thus we report detailed studies
of inclusive cross sections for inelastic He(p,p')He and
charge-exchange Li(n,p)He reactions at nucleon energy 50 MeV. The
theoretical low-energy spectra exhibit two resonance-like structures. The first
(narrow) is the excitation of the well-known three-body resonance. The
second (broad) bump is a composition of overlapping soft modes of
multipolarities whose relative weights depend on
transferred momentum and reaction type. Inelastic scattering is the most
selective tool for studying the soft dipole excitation mode.Comment: Submitted to Phys. Rev. C., 11 figures using eps
Nuclear structure studies with the 7Li(e,e'p) reaction
Experimental momentum distributions for the transitions to the ground state
and first excited state of 6He have been measured via the reaction
7Li(e,e'p)6He, in the missing momentum range from -70 to 260 MeV/c. They are
compared to theoretical distributions calculated with mean-field wave functions
and with variational Monte Carlo (VMC) wave functions which include strong
state-dependent correlations in both 7Li and 6He. These VMC calculations
provide a parameter-free prediction of the momentum distribution that
reproduces the measured data, including its normalization. The deduced summed
spectroscopic factor for the two transitions is 0.58 +/- 0.05, in perfect
agreement with the VMC value of 0.60. This is the first successful comparison
of experiment and ab initio theory for spectroscopic factors in p-shell nuclei.Comment: 4 pages, 3 figure
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