Food Price Volatility in Ethiopia: Public Pressure and State Response

The global market, variable agricultural production and irregular trading practices have marked food price volatility in Ethiopia over the last decade. However, the recent decline in global prices of food and fuel, coupled with state intervention in managing the supply of consumer goods, have brought some stability to food prices in 2014/15. While the safety net and price control measures could help mitigate the aggravation of impacts of food price increases on poor families, a more comprehensive food security approach is necessary. The article argues the importance of enhancing the purchasing power of the people

Level of satisfaction of disabled people with the tax breaks given to them

This study aimed to verify the level of satisfaction of persons with disabilities with the tax exemptions provided to them, and the problem of the study is in the following question: What is the level of satisfaction of persons with disabilities with the tax exemptions provided to them? The researchers relies on the study hypotheses that there are no statistical differences in the level of satisfaction of persons with disabilities with the tax exemptions provided to them on the content of the study tool and according to its variables (gender, age, and educational qualification). To achieve the goal of this study, the researchers used a main method, which is the descriptive study, based on a questionnaire that prepared to collect information. The study population consisted of a group of persons with disabilities in the West Bank governorates, who number (44570) disabled. As for the sample, it was applied to the governorates of the West Bank, and it consisted of (180) persons with disabilities from the age of 18 years or more, and (178) of them were retrieved. This is what constituted the final sample of the study, as (0.4%) of the study population. This study reached to the following result: The level of satisfaction of persons with disabilities with the tax exemptions provided to them reach an arithmetic average of two study axes (56%). The most important recommendations recommended by the researchers: the necessity of working to increase tax awareness for persons with disabilities in order to know their rights and duties, they must work to reformulate tax laws and include the issue of persons with disabilities within the policy of the state, attention to integrating persons with disabilities in working life more, Because of its benefit to the handicapped and society, this study was conducted repeatedly to verify the level of satisfaction of persons with disabilities with the tax exemptions presented to them to ensure that these exemptions achieve the goal for which they were set for it

Managing Environmental Risk in Presence of Climate Change: The Role of Adaptation in the Nile Basin of Ethiopia

This study investigates the impact of climate change adaptation on farm households' downside risk exposure in the Nile Basin of Ethiopia. The analysis relies on a moment-based specification of the stochastic production function. We use an empirical strategy that accounts for the heterogeneity in the decision on whether to adapt or not, and for unobservable characteristics of farmers and their farm. We find that past adaptation to climate change (i) reduces current downside risk exposure, and so the risk of crop failure; (ii) would have been more beneficial to the non-adapters if they adapted, in terms of reduction in downside risk exposure; and (iii) is a successful risk management strategy that makes the adapters more resilient to climatic conditions

Genetically Engineered Hepatitis C Virus-like Particles (HCV-LPs) Tagged with SP94 Peptide to Acquire Selectivity to Liver Cancer Cells via Grp78

Targeted cancer therapy is a challenging area that includes multiple chemical and biological vehicles. Virus-like particles (VLPs) combine safety and efficacy in their roles as potential vaccines and drug delivery vehicles. In this study, we propose a novel drug delivery system based on HCV-LPs engineered with SP94 and RGD peptides mediated by a specific molecular chaperone (Grp78) associated with cancer drug resistance. The PCR primers were designed for engineering two constructs, SP94-EGFP-CORE-HIS and RGD-EGFP-CORE-HIS, by sequential PCR reactions. The two fragments were cloned into pFastBac Dual under the polyhedrin promoter and then used to produce two recombinant baculoviruses (AcSP94 and AcRGD). The VLP’s expression was optimized by recombinant virus infection with different MOIs, ranging from 1 to 20 MOI. Recombinant VLP2 were purified by Ni-NTA and their sizes and shapes were confirmed with TEM. They were incubated with different types of cells prior to examination using the fluorescence microscope to test the binding specificity. The effect of the overexpression of the Grp78 on the binding affinity of the engineered VLPs was tested in HepG2 and HeLa cells. The protocol optimization revealed that MOI 10 produced the highest fluorescence intensities after 72 h for the two recombinant proteins (SP94-core and RGD-core). Moreover, the binding assay tested on different types of mammalian cells (HeLa, HEK-293T, and HepG2 cells) showed green fluorescence on the periphery of all tested cell lines when using the RGD-core protein; while, the SP94-core protein showed green fluorescence only with the liver cancer cells, HepG2 and HuH7. Overexpression of Grp78 in HepG2 and HeLa cells enhanced the binding efficiency of the engineered VLPs. We confirmed that the SP94 peptide can be specifically used to target liver cancer cells, while the RGD peptide is sufficiently functional for most types of cancer cells. The overexpression of the Grp78 improved the binding capacity of both SP94 and RGD peptides. It is worth noting that the SP94 peptide can function properly as a recombinant peptide, and not only as a chemically conjugated peptide, as heretofore commonly used

The Transgene Expression of the Immature Form of the HCV Core Protein (C191) and the LncRNA MEG3 Increases Apoptosis in HepG2 Cells

Long non-coding RNAs (lncRNAs) are regulated in cancer cells, including lncRNA MEG3, which is downregulated in Hepatocellular Carcinoma (HCC). In addition, hepatitis C virus (HCV) core proteins are known to dysregulate important cellular pathways that are linked to HCC development. In this study, we were interested in evaluating the overexpression of lncRNA MEG3, either alone or in combination with two forms of HCV core protein (C173 and C191) in HepG2 cells. Cell viability was assessed by MTT assay. Transcripts’ levels of key genes known to be regulated in HCC, such as p53, DNMT1, miRNA152, TGF-b, and BCL-2, were measured by qRT-PCR. Protein expression levels of caspase-3 and MKI67 were determined by immunocytochemistry and apoptosis assays. The co-expression of lncRNA MEG3 and C191 resulted in a marked increase and accumulation of dead cells and a reduction in cell viability. In addition, a marked increase in the expression of tumor suppressor genes (p53 and miRNA152), as well as a marked decrease in the expression of oncogenes (DNMT1, BCL2, and TGF-b), were detected. Moreover, apoptosis assay results revealed a significant increase in total apoptosis (early and late). Finally, immunocytochemistry results detected a significant increase in apoptotic marker caspase-3 and a decrease in tumor marker MKI67. In this study, transgene expression of C191 and lncRNA MEG3 showed induction in apoptosis in HepG2 cells greater than the expression of each one alone. These results suggest potential anticancer characteristics