Metal content in fruit-bodies and mycorrhizas of Pisolithus arrhizus from zinc wastes in Poland

Pisoliihus arrhizus has been selected for investigation as one of the ectomycorrhizal species most resistant to stress factors. Metal content in fruit-bodies and mycorrhizas was estimated to evaluate their role as bioindicators and to check whether mycorrhizas have any special properties for heavy metal accumulation. Fruit-bodies and mycorrhizas were collected from zinc wastes in Katowice-Wehoswicc and analyzed using conventional atomic absorption spectroscopy and energy dispersive spectroscopy accompanying scanning electron microscopy. Differences in tendencies to accumulate metals within sporophorea and mycorrhizas were found.. The fruR-bodies accumulated Al (up to 640 pgg*'l, while high concentrations of Al, Zn, Fe. Ca and Si were noted in the outer mantle of the mycorrhizas, in the material secreted and in the mycelium wall. The content of elements varied depending on the age of mycorrhizas. The ability of extramatrieal mycelium and hyphae forming mycorrhizal mantle to immobilize potentially toxic el emenis might indicate biofiltering properties though the next step should include investigations on ability of the fungus to prevent element uptake by the plant

Hepcidin is regulated by promoter-associated histone acetylation and HDAC3.

Hepcidin regulates systemic iron homeostasis. Suppression of hepcidin expression occurs physiologically in iron deficiency and increased erythropoiesis but is pathologic in thalassemia and hemochromatosis. Here we show that epigenetic events govern hepcidin expression. Erythropoiesis and iron deficiency suppress hepcidin via erythroferrone-dependent and -independent mechanisms, respectively, in vivo, but both involve reversible loss of H3K9ac and H3K4me3 at the hepcidin locus. In vitro, pan-histone deacetylase inhibition elevates hepcidin expression, and in vivo maintains H3K9ac at hepcidin-associated chromatin and abrogates hepcidin suppression by erythropoietin, iron deficiency, thalassemia, and hemochromatosis. Histone deacetylase 3 and its cofactor NCOR1 regulate hepcidin; histone deacetylase 3 binds chromatin at the hepcidin locus, and histone deacetylase 3 knockdown counteracts hepcidin suppression induced either by erythroferrone or by inhibiting bone morphogenetic protein signaling. In iron deficient mice, the histone deacetylase 3 inhibitor RGFP966 increases hepcidin, and RNA sequencing confirms hepcidin is one of the genes most differentially regulated by this drug in vivo. We conclude that suppression of hepcidin expression involves epigenetic regulation by histone deacetylase 3.Hepcidin controls systemic iron levels by inhibiting intestinal iron absorption and iron recycling. Here, Pasricha et al. demonstrate that the hepcidin-chromatin locus displays HDAC3-mediated reversible epigenetic modifications during both erythropoiesis and iron deficiency

Hepcidin is regulated by promoter-associated histone acetylation and HDAC3

Hepcidin regulates systemic iron homeostasis. Suppression of hepcidin expression occurs physiologically in iron deficiency and increased erythropoiesis but is pathologic in thalassemia and hemochromatosis. Here we show that epigenetic events govern hepcidin expression. Erythropoiesis and iron deficiency suppress hepcidin via erythroferrone-dependent and -independent mechanisms, respectively, in vivo, but both involve reversible loss of H3K9ac and H3K4me3 at the hepcidin locus. In vitro, pan-histone deacetylase inhibition elevates hepcidin expression, and in vivo maintains H3K9ac at hepcidin-associated chromatin and abrogates hepcidin suppression by erythropoietin, iron deficiency, thalassemia, and hemochromatosis. Histone deacetylase 3 and its cofactor NCOR1 regulate hepcidin; histone deacetylase 3 binds chromatin at the hepcidin locus, and histone deacetylase 3 knockdown counteracts hepcidin suppression induced either by erythroferrone or by inhibiting bone morphogenetic protein signaling. In iron deficient mice, the histone deacetylase 3 inhibitor RGFP966 increases hepcidin, and RNA sequencing confirms hepcidin is one of the genes most differentially regulated by this drug in vivo. We conclude that suppression of hepcidin expression involves epigenetic regulation by histone deacetylase 3.Hepcidin controls systemic iron levels by inhibiting intestinal iron absorption and iron recycling. Here, Pasricha et al. demonstrate that the hepcidin-chromatin locus displays HDAC3-mediated reversible epigenetic modifications during both erythropoiesis and iron deficiency

Chatbot as a Tool for Knowledge Sharing in the Maintenance and Repair Processes

In the era of digitization and general access to Internet resources, it seems natural to expect fast, direct, and tailored access to the knowledge of interest. The research shows that the use of modern technologies is not reserved only for industrial solutions, but can be applied in common use. Solutions based on artificial intelligence can provide solutions to many problems of the modern world. This is particularly visible in the era of challenges related to ecology (e.g. minimization of paper consumption) and expectations of fast access to information regardless of place and time. Treating chatbots as a tool supporting the customer in current activities, quick problem-solving shows the potential and care for the image of the producers. Developing the functionality of virtual assistants used for marketing activities with the functionality of technical support can bring tangible benefits both in the economic and social spheres. It should be noted that electronic versions of documents, knowledge transferred in an interactive form can be easily updated and presented in different forms. This increases the attractiveness of transmitted knowledge, users can choose the appropriate form, and access to these resources becomes easier. Artificial intelligence-based tools in the form of chatbots (also called virtual assistants) seem to be a solution worthy of interest. This paper presents the process of developing a virtual assistant to support the maintenance and repair activities of small household appliances. A chatbot aims at gathering in one place and then providing dedicated knowledge resources to an individual user on a just-in-time basis

Air temperature and air humidity variability in Krakow in 2008 (based on data collected by the III Kampus UJ automated meteorology station).

W pomiarach meteorologicznych coraz częściej korzysta się z automatycznych stacji meteorologicznych. Dużą zaletą takich stacji jest możliwość ciągłego pomiaru bez udziału obserwatora. Dzięki otrzymywaniu danych w formie cyfrowej mogą być ona na bieżąco publikowane oraz udostępniane. Dane z automatów są jednak nieporównywalne z danymi ze stacji standardowych, gdyż brakuje ujednoliconych procedur pomiarowych. Niniejsza praca stanowi próbę szczegółowego opracowania danych z automatycznej stacji meteorologicznej III Kampus UJ. Głównym celem pracy jest ukazanie zmienności czasowej temperatury i wilgotności względnej powietrza w Krakowie w 2008 roku oraz zbadanie wpływu cyrkulacji atmosfery na tę zmienność. Zanalizowane zostały wartości średnie i skrajne, a także przebieg roczny, dobowy i godzinny obu analizowanych elementów meteorologicznych. Wydzielono także dni charakterystyczne oraz przedstawiony został wpływ typów cyrkulacji na zmienność temperatury i wilgotności powietrza w Krakowie. Analiza danych ze stacji III Kampus UJ wykazała, że rok 2008 był wyjątkowo ciepły i wilgotny. Świadczy o tym wysoka średnia roczna wartość temperatury (10,4°C) oraz wilgotności względnej powietrza (80%) w stosunku do średnich wieloletnich z XX wieku. Liczba dni upalnych i gorących była wyższa niż średnia wieloletnia, natomiast liczba dni mroźnych niższa. Ponadto dni suchych było mniej niż średnio w XX wieku, za to dni bardzo wilgotnych więcej. Dane ze stacji automatycznych dają dużo większe możliwości podczas analizy niż te ze stacji tradycyjnych. Możliwe jest szczegółowe przedstawienie wartości w przebiegu godzinnym, albo w wybranych terminach. Można również określić dokładny termin wystąpienia wartości skrajnych co nie jest możliwe przy korzystaniu z danych ze stacji tradycyjnych.Automated meteorology stations, used for meteorological measurements are becoming more common. Their greatest advantage is the ability to constantly operate without the need for a person to be present at the station while it is taking measurements. The gathered information can later be accessed and published in digital form. These automated stations however cannot be compared to traditional stations, because they lack uniform measurement procedures.In this thesis paper I look at data and elaborate on data collected by the III Kampus UJ automated meteorology station. The main purpose is to obtain the temperature differentiation in time and relative air humidity in Krakow in the year 2008 and also to determine the impact of atmospheric circulation on these variables. Median and extreme values were taken into account in yearly, daily and hourly intervals, for both variables. Characteristic days were determined and I present the impact of different types of atmosphere circulation on the air temperature differentiation and relative humidity.The analyzed data from the III Kampus UJ station show that the year 2008 was hot and humid. This was determined by the high year average temperature value (10,4°C) and the relative air humidity (80%) compared to the averages throughout many years in the XX century. The number of hot days in 2008 was higher than for the average throughout the XX century, whereas the number of cold days was lower. A similar correlation can be observed for humid days. The number of days with high humidity was greater, and the number of dry days was lower, when compared to the XX century average. Automated meteorological stations give many more possibilities during data analysis when compared to traditional stations. It is possible to present the obtained data in a timeline that can for example be hourly or stretched over a longer period of time. It is also possible to determine exactly when extreme condition occurred which is not possible when using traditional stations

Verification of Work Ergonomics at Selected Workstations on the Assembly Line – Planning Versus Reality

Analysis of working conditions in terms of ergonomics is an increasingly popular topic among industrial enterprises. This is primarily due to the development of departments responsible for maintaining safe and healthy working conditions, but also awareness of the impact of working conditions on maintaining safety and maintaining the rhythm of work. Care for working conditions translates into the possibility of maintaining the level of activities performed, which indirectly affects the quality of final products. Conducting activities in the field of shaping ergonomic working conditions in the context of continuous improvement based on the Deming cycle and the principles of conceptual ergonomics seem to be a key approach to care for work safety. Treating this process as a continuous and looped process gives you the ability to react quickly and predict adverse effects. However, constant control, observation, and analysis of the possibilities of implementing improvement solutions is important. The purpose of the article is to compare ergonomic working conditions at selected work stations with the assumptions and plans developed before launching the assembly line. The research included a comparative analysis of results in the form of ergonomic score obtained from TiCon software, based on MTM, UAS, and EAWS methods. The research was carried out at the turn of 2016-2020 in an automotive company dealing in the production of passenger car drive system components