58 research outputs found
Five-point Likert scaling on MRI predicts clinically significant prostate carcinoma
Background: To clarify the relationship between the probability of prostate cancer scaled using a 5-point Likert system and the biological characteristics of corresponding tumor foci. Methods: The present study involved 44 patients undergoing 3.0-Tesla multiparametric MRI before laparoscopic radical prostatectomy. Tracing based on pathological and MRI findings was performed. The relationship between the probability of cancer scaled using the 5-point Likert system and the biological characteristics of corresponding tumor foci was evaluated. Results: A total of 102 tumor foci were identified histologically from the 44 specimens. Of the 102 tumors, 55 were assigned a score based on MRI findings (score 1: nā=ā3; score 2: nā=ā3; score 3: nā=ā16; score 4: nā=ā11 score 5: nā=ā22), while 47 were not pointed out on MRI. The tracing study revealed that the proportion of >0.5 cm3 tumors increased according to the upgrade of Likert scores (score 1 or 2: 33 %; score 3: 68.8 %; score 4 or 5: 90.9 %, Ļ2 test, pā7 also increased from scale 2 to scale 5 (scale 2: 0 %; scale 3: 56.3 %; scale 4: 72.7 %; 5: 90.9 %, Ļ2 test, pā=ā0.0001). On using score 3 or higher as the threshold of cancer detection on MRI, the detection rate markedly improved if the tumor volume exceeded 0.5 cm3 (<0.2 cm3: 10.3 %; 0.2-0.5 cm3: 25 %; 0.5-1.0 cm3: 66.7 %; 1.0ā<ācm3: 92.1 %). Conclusions: Each Likert scale favobably reflected the corresponding tumorās volume and Gleason score. Our observations show that āscore 3 or higherā could be a useful threshold to predict clinically significant carcinoma when considering treatment options
Facile synthesis of C-terminal peptide thioacids under mild conditions from N-sulfanylethylanilide peptides
A facile procedure has been developed for the synthesis of C-terminal peptide thioacids under mild conditions. A series of N-sulfanylethylanilide peptides prepared using Fmoc-based solid-phase peptide synthesis were successfully converted to the corresponding thioacids via a hydrothiolysis reaction in a phosphate buffer with only trace epimerization of the C-terminal amino acid
Physiological Markers of Motor Improvement Following Five-month Sprint Training in Young Boys
The 11th International Symposium on Adaptive Motion of Animals and Machines. Kobe University, Japan. 2023-06-06/09. Adaptive Motion of Animals and Machines Organizing Committee.Poster Session P4
Development of anĀ epileptic seizure prediction algorithm using RāR intervals with self-attentive autoencoder
Epilepsy is a neurological disorder that may affect the autonomic nervous system (ANS) from 15 to 20 min before seizure onset, and disturbances of ANS affect RāR intervals (RRI) on an electrocardiogram (ECG). This study aims to develop a machine learning algorithm for predicting focal epileptic seizures by monitoring RāR interval (RRI) data in real time. The developed algorithm adopts a self-attentive autoencoder (SA-AE), which is a neural network for time-series data. The results of applying the developed seizure prediction algorithm to clinical data demonstrated that it functioned well in most patients; however, false positives (FPs) occurred in specific participants. In a future work, we will investigate the causes of FPs and optimize the developing seizure prediction algorithm to further improve performance using newly added clinical data
Asymmetric Hsp90 N domain SUMOylation recruits Aha1 and ATP-competitive inhibitors
The stability and activity of numerous signaling proteins in both normal and cancer cells depends on the dimeric molecular chaperone heat shock protein 90 (Hsp90). Hsp90's function is coupled to ATP binding and hydrolysis and requires a series of conformational changes that are regulated by cochaperones and numerous posttranslational modifications (PTMs). SUMOylation is one of the least-understood Hsp90 PTMs. Here, we show that asymmetric SUMOylation of a conserved lysine residue in the N domain of both yeast (K178) and human (K191) Hsp90 facilitates both recruitment of the adenosine triphosphatase (ATPase)-activating cochaperone Aha1 and, unexpectedly, the binding of Hsp90 inhibitors, suggesting that these drugs associate preferentially with Hsp90 proteins that are actively engaged in the chaperone cycle. Importantly, cellular transformation is accompanied by elevated steady-state N domain SUMOylation, and increased Hsp90 SUMOylation sensitizes yeast and mammalian cells to Hsp90 inhibitors, providing a mechanism to explain the sensitivity of cancer cells to these drugs. Ā© 2014 Elsevier Inc
Ramucirumab plus docetaxel versus placebo plus docetaxel in patients with locally advanced or metastatic urothelial carcinoma after platinum-based therapy (RANGE): a randomised, double-blind, phase 3 trial
Few treatments with a distinct mechanism of action are available for patients with platinum-refractory advanced or metastatic urothelial carcinoma. We assessed the efficacy and safety of treatment with docetaxel plus either ramucirumab-a human IgG1 VEGFR-2 antagonist-or placebo in this patient population
Ubiquitylation of epsilon-COP by PIRH2 and regulation of the secretion of PSA
Ubiquitylation appears to be involved in the membrane trafficking system including endocytosis, exocytosis, and ER-to-Golgi transport. We found that PIRH2, which was identified as an interacting protein for androgen receptor or p53, interacts with and ubiquitylates the Īµ-subunit of coatmer complex, Īµ-COP. PIRH2 promotes the ubiquitylation of Īµ-COP in vitro and in vivo and consequently promotes the degradation of Īµ-COP. The interaction between PIRH2 and Īµ-COP is affected by the presence of androgen, and PIRH2 in the presence of androgen promotes ubiquitylation of Īµ-COP in vivo. Furthermore, overexpression of the wild type of PIRH2 in prostate cancer cells causes downregulation of the secretion of prostate-specific antigen (PSA), a secretory protein in prostate epithelial cells and one of diagnostic markers for prostate cancer. Our results indicate that PIRH2 functions as a regulator for COP I complex
Ligand-dependent transcription of estrogen receptor Ī± is mediated by the ubiquitin ligase EFP
Estrogen-mediated ubiquitylation and subsequent degradation of the estrogen receptor Ī± (ERĪ±) appears to be involved in the transcriptional activity of ERĪ±. We show that the estrogen-responsive finger protein (EFP) interacts with and ubiquitylates ERĪ±. EFP promoted the ubiquitylation of ERĪ± in vitro and in vivo and consequently promoted the degradation of ERĪ±. The interaction between EFP and ERĪ± was greatly enhanced in the presence of estrogen. The action of EFP on ERĪ± in the presence of estrogen resulted in a robust interaction between ERĪ± and Tip60, one of the transcriptional coactivators, leading to activation of ERĪ± transcriptional activity. However, a dominant negative mutant of EFP lacking the RING domain prolonged the half-life of ERĪ± and inhibited the transcription by ERĪ±. Our results indicate that EFP functions as a cofactor for ERĪ±-mediated transcription, thus suggesting that ERĪ±-mediated transcription is closely linked to the ubiquitylation of ERĪ±
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