Marsupialization of mandibular cystic lesions, clinical observations of 23 cases

1. Marsupialization was performed on 23 cases of cystic lesions of the jaw at the Department of Oral Surgery, Okayama University Hospital in the recent five years. 2. These patients were divided into 4 groups according to their age; namely, group A of those under IS years old, group B of those between 15 and 30 years old, group C of those between 31 and 60 years old, and group D of those over 61 years old, and the results of postoperative findings were compared with those of preoperative ones. 3. In group A of the four groups the most favorable results were obtained after marsupialization and reduction of the tumor was remarkable, even in a case of ameloblastoma, as compared with groups B, C and D. 4. Cure of lesions took somewhat a longer period of time in group B than in group A, but all the cystic lesions were reduced favorably after operation. 5. The reduction in cysts in groups C and D was markedly slower when compared with that in groups A and B, but the marsupialization surgery seems to be desirable in some cases.</p

Targeted gene integration using the combination of a sequence-specific DNA-binding protein and phiC31 integrase.

PhiC31 integrase-based vectors can integrate therapeutic genes selectively into attP or pseudo-attP sites in genomes, but considerable numbers of pseudo-attP sites in human genomes exist inside endogenous gene-coding regions. To avoid endogenous gene disruptions, we aimed to enhance the integration site-specificity of the phiC31 integrase-based vector using a sequence-specific DNA-binding protein containing Gal4 and LexA DNA-binding motifs. The dual DNA-binding protein was designed to tether the UAS-containing donor vector to the target sequence, the LexA operator, and restrict integration to sites close to the LexA operator. To analyze the site-specificity in chromosomal integration, a human cell line having LexA operators on the genome was established, and the cell line was transfected with donor vectors expressing the DNA-binding protein and the phiC31 integrase expression vector (helper vector). Quantitative PCR indicated that integration around the LexA operator was 26-fold higher with the UAS-containing donor vector than with the control. Sequence analysis confirmed that the integration occurred around the LexA operator. The dual DNA-binding protein-based targeted integration strategy developed herein would allow safer and more reliable genetic manipulations for various applications, including gene and cell therapies

piggyBac Transposon-mediated Long-term Gene Expression in Mice

Transposons are promising systems for somatic gene integration because they can not only integrate exogenous genes efficiently, but also be delivered to a variety of organs using a range of transfection methods. piggyBac (PB) transposon has a high transposability in mammalian cells in vitro, and has been used for genetic and preclinical studies. However, the transposability of PB in mammalian somatic cells in vivo has not been demonstrated yet. Here, we demonstrated PB-mediated sustained gene expression in adult mice. We constructed PB-based plasmid DNA (pDNA) containing reporter [firefly and Gaussia luciferase (Gluc)] genes. Mice were transfected by injection of these pDNAs using a hydrodynamics-based procedure, and the conditions for high-level sustained gene expression were examined. Consequently, gene expressions were sustained over 2 months. Our results suggest that PB is useful for organ-selective somatic integration and sustained gene expression in mammals, and will contribute to basic genetic studies and gene therapies

La interculturalidad como orientación metodológica y de análisis en la comunicación organizacional

A complexidade do ambiente organizacional aumenta devido às transformações econômicas, sociais e culturais. Isso é impulsionado pelos avanços da globalização e da internacionalização, além da evolução tecnológica. Nesse cenário, a interculturalidade evidencia a necessidade de aprimorar os métodos da comunicação organizacional, visando imprimir efetividade em suas estratégias e práticas. Neste artigo, é apresentada uma proposta metodológica, embasada no estudo da interface Comunicação e Tecnologias da Informação, pautada na observação, pesquisas e análises, visando a “comunicação excelente” por meio das “expectativas compartilhadas”.The complexity of the organizational environment increases due to economic, social and cultural transformations. This is driven by the advances of globalization and internationalization, apart from technological developments. In this scenario, the interculturalism emphasizes the need of efficient methods of organizational communication in order to print more effectiveness in their strategies and practices. This article presents a methodology, grounded on the study about Communication and Information Technology interface, based on observation, research and analysis, aiming to "excellent communication" by means of "shared expectations".La complejidad del entorno organizacional aumenta debido a las transformaciones económicas, sociales y culturales. Esto es impulsado por los avances de la globalización y la internacionalización, además de la evolución tecnológica. En este escenario, la interculturalidad enfatiza la necesidad de métodos eficaces de la comunicación organizacional con el fin de imprimir más eficacia sus estrategias y prácticas. En este artículo se presenta una metodología, fundada en el estudio sobre el interfaz de Tecnologías de la Información y Comunicación, basada en la observación, la investigación y el análisis, con el objetivo de "una excelente comunicación" por medio de "expectativas compartidas