Atividade dissacaridásica intestinal da esquistossomose mansônica: estudo evolutivo em camundongos com diferentes cargas de infestação

A esquistossomose mansônica compromete vários órgãos, sendo o intestino e o fígado os mais agredidos. Com a intenção de verificar o comprometimento do intestino delgado, dependente da intensidade e do tempo de infecção pelo Schistosoma mansoni, analisou-se a atividade das dissacaridases lactase, sacarase e maltase em 112 camundongos, distribuídos em 3 grupos: grupo I controle, grupo II infestado com 30 cercárias, grupo III infestado com 60 cercárias. Observamos uma diminuição da atividade lactásica, sacarásica e maltásica do intestino delgado, decorrente da infestação esquistossomdtica, do tempo de infestação e da alteração entre ambos. O íleo é o segmento que demonstrou maior sensibilidade a esquistossomose, tendo uma diminuição das suas dissacaridases a partir da fase inicial de infestação. Opostamente, o jejuno só mais tardiamente mostra essas alterações, exceto em relação a lactase. Detectou-se um aumento da atividade dissacaridásica, inclusive para a lactase, em todos os grupos, com a evolução etária dos animais, quantitativamente menor nos infestados. Cargas de 30 e 60 cercárias devem ser consideradas de mesmo porte, pois produziram ledução semelhante na atividade dissacaridásica.Schistosomiasis mansoni attacks a number of organs, of which the intestine and liver are the most affected. This analysis was undertaken in order to verify the degree to which the small intestine is attacked, in accordance with the intensity and duration of infestation by Schistosoma mansoni; it focused on the activity of disaccharidase lactase, saccharase and maltase in 112 mice, divided into 3 groups: group I, for control; group II, infested with 30 cercariae; and group III, infested with 60 cercariae. We observed a fall in lactase, saccharase and maltase activity in the small intestine, as a result of infestation by schistosomiasis, of the duration of infestation and of the interaction between the two. The ileum was the section showing greatest sensitivity to schistosomiasis, and its disaccharidases fell off right from the start of infestation. On the other hand, the jejunum only showed such alterations much later on, except as regards lactase. An increase in disaccharidase activity was detected in all groups as the age of the animals increased, but this was quantitatively lower in the infested animals. Infestation with 30 and 60 cercariae should he seen as of the same degree, as they produced similar reductions in disaccharidase activity

VESTIBULAR DISORDERS CAUSED BY DEFECTIVE ENZYME MECHANISMS in the SMALL-INTESTINE

ESCOLA PAULISTA MED,DEPT OTOLARYNGOL,BR-04023 São Paulo,SP,BRAZILESCOLA PAULISTA MED,DEPT CLIN GASTROENTEROL,BR-04023 São Paulo,SP,BRAZILESCOLA PAULISTA MED,DEPT OTOLARYNGOL,BR-04023 São Paulo,SP,BRAZILESCOLA PAULISTA MED,DEPT CLIN GASTROENTEROL,BR-04023 São Paulo,SP,BRAZILWeb of Scienc

Parasite and Egg Burden, Hepatic Collagen and Histologic Pattern of Liver Granulomas in Selection III High and Low Antibody Responder Mice Infected with Schistosoma mansoni

Selection III mice have particular immunological characteristics: they are high (H 111) or low (L 111) antibody producer animals, yet both lines display similar T cell responses and macrophage activities. We submitted these mice to infection with Schistosoma mansoni 10 assess in vivo parasite and egg burden, hepatic collagen and cellular composition of granulomas in both lines. Titration of anti-Schistosoma IgG by ELISA showed remarkably higher values in H III line, at both studied periods (8th and 12th weeks post-infection). Nevertheless, the number of adult worms recovered from the portal system was similar in both lines, being not associated with anti-Schistosoma antibody levels. There is an increase in hepatic collagen from the 8th to the 12th weeks post-infection, which is paralleled by an increase in the number of eggs in the liver. This association apparently occurs at the same ratio in H III and L III animals. The most important difference found between the two lines was lhe outstanding contrast in terms of volume and eosinophil counts in the granulomas, with lesions from H III mice clearly being larger and containing more of these cells than LIII lesions