ANTI MICROBIAL EFFICACY OF CINNAMOMUM ZEYLANICUM

Cinnamomum Zeylanicum has antimicrobial property and has been studied against different diseses causing organism.Its leaf as well as the bark has antimicrobial properties in their oils .In the research study bark oil was found to be more effective

EFFECT OF COFFEE ON MICRO ORGANISM

The research work in this paper is on effect on coffee on the growth of microorganism. Caffeine is the active component of coffee .I t is a plant alkaloid which is found in more than 60 plant species just like coffia arabica (Coffee), Thea sinensis (Tea plant).It is plant product that is most commonly found in coffee beans, tea, cocoa and chocolates. Caffeine is also found in some prescription and non-prescription drug including cold, allergy and pain relievers. And it is mostly used by human. In this experiment we extract the caffeine from coffee powder using organic solvent (Ethyl acetate). The effect of caffeine was studied on Bacillus cereus. The caffeine proved the result show as Antibacterial agent

PURIFICATION AND CHARACTERIZATION OF a-GLUCOSIDASE FROM MOSS HYOPHILLA NYMANIANA (FLEISH.) MENZEL

Objective: The present study was undertaken to extract and purify α-glucosidase N-linked glycosylation enzyme from moss Hyophilla nymaniana(Fleish.) Menzel.Methods: Frozen protonemal cells were taken for crude enzyme extraction, and the enzyme α-glucosidase was purified from the prepared crudeenzyme extract by ammonium sulfate (NH4) 2SO4 precipitation, gel filtration and finally on diethylaminoethyl sephadex column chromatography.Results: The final purification step of the enzyme resulted in 35 fold purification with a recovery of 4%. A single protein band of 72±5 kilodalton wasseen on 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The physiochemical characterization of the enzyme reveled the enzyme hada wide pH stability range 4-7 with optimum pH 5 while the temperature stability study revealed the enzyme was stable up to 60°C while the optimumtemperature of the purified enzyme was 45°C. The enzyme was strongly inhibited by Hg2+ and Ag2+ at 1 mM concentration while Mg ionsenhanced the enzyme activity at the same concentration. The kinetic study of the enzyme showed Km and V of the enzyme 5.2 mM/ml and 8.6 U/ml,respectively.Conclusion: The wide pH and temperature stability range show its suitability toward industrial application.maxKeywords: α-glucosidase, Hyophilla nymaniana (Fleish.) Menzel. gel filtration, Diethylaminoethyl sephadex column chromatography

Design of Some Benzimidazoles as Target for α-Glucosidase Inhibitors

Diabetes mellitus is rising globally touching more than 180 million people worldwide. This is prevailing mostly in type 2 diabetes and according to WHO report the incidence is likely to be more than doubled by 2030. α-Glucosidase inhibitors work by reducing the amount of glucose that the intestines absorb from food. In this work, forty-five benzimidazole analogues were studied using 3D QSAR, HQSAR, pharmacophore mapping and based on their results 60 compounds were designed. The results show that the best Comparative Molecular Field Analysis (CoMFA) model has q2 = 0.742 and r2 = 0.973, and the best Comparative Molecular Similarity Indices Analysis (CoMSIA) model has q2 = 0.679 and r2 = 0.918. For HQSAR the best model has q2 = 0.773 and r2 = 0.964. The r2 value for boostrap for CoMFA and CoMSIA are 0.98 and 0.97 respectively. Pharmacophore mapping revealed varied bioactive regions of ligand. Thus, these compounds could be used as lead for designing the synthesis of potent alpha-glucosidase inhibitors. Keywords: Acarbose, Alpha-glucosidase inhibition, Benzimidazoles, Molecular modelling, Post-prandial hyperglycemi

Synthesis and Biological Evaluation of Benzimidazoles as Target for α-Glucosidase Inhibitors

Diabetes mellitus is rising globally touching more than 180 million people worldwide. This is prevailing mostly in type 2 diabetes and according to WHO report the incidence is likely to be more than doubled by 2030. α-glucosidase inhibitors work by reducing the amount of glucose that the intestines absorb from food. In our previous work, forty-five benzimidazoles analogues were studied using 3D QSAR, HQSAR, and Pharmacophore mapping and based on their results 60 compounds were designed. Docking studies of those designed compounds showed that most of the compounds are bonding with important amino acids LEU 520, ARG 335 and ASP 69 through hydrogen bonds and steric interaction. In this work, synthesis of eleven compounds was done on the basis of molecular docking studies. Compounds containing hydroxyl and alkyl groups (compound no. 3, 9 and 10) were found to be five to eight folds more active with IC90 values in the range of 6.02 ± 1.10 to 33.25 ± 1.20 µg/ml, in comparison with the standard drug, Acarbose (IC90= 290.55 ± 0.081 µg/ml). Thus, these compounds after the toxicity studies could be of therapeutic use in treating diabetes. Keywords: Acarbose, Alpha-glucosidase inhibition, Benzimidazoles, Docking, Molecular modelling, Post-prandial hyperglycemi

Supply Chain Resilience Meets Quality Management

Purpose: This study is aimed at supply chain resilience and quality management, which are critical in today's volatile global economy marked by various disruptions. The focus is on how these traditionally separate disciplines can be combined to enhance organizational performance.   Theoretical framework:  The theoretical framework revolves around the concepts of supply chain resilience, which includes elements like proactive risk assessment, adaptability, collaborative networks, strategic redundancies, and quality management principles. The research scrutinizes their interplay, revealing significant overlap and implications for organizational outcomes.   Design/Methodology/Approach:  An exploratory case study approach forms the core methodology, primarily utilizing secondary data to gain insights into real-world scenarios. This approach enables a deep understanding of the critical factors influencing supply chain resilience and the impact of quality management concepts on customer satisfaction and product/service quality in supply chain processes. The questionnaire is distributed to get insights into the company's practices.   Findings:  The findings indicate that integrating supply chain resilience with quality management leads to more sustained and improved organizational outcomes. A resilient supply chain is quicker to recover from disruptions, minimizing financial impacts. Concurrently, a steadfast focus on quality ensures customer satisfaction and loyalty. This integration offers synergistic benefits, contributing to operational efficiency, market competitiveness, and a more substantial brand reputation.   Research, Practical & Social implications: The study provides insights into critical factors of supply chain resilience and their interconnections with quality management concepts. These insights can inform strategies for enhancing resilience and quality management in supply chains. Practically, the study offers a guide for organizations to adopt a unified approach that optimizes supply chain resilience and quality management. This approach is particularly relevant for organizations seeking to improve operational efficiency and customer satisfaction through better risk management and quality assurance processes. Socially, the study highlights the importance of resilient and high-quality supply chains in today’s business landscape by maintaining Sustainable Developmental Goals.   Originality/Value:  This research highlights how resilience, characterized by adaptability and robust recovery in disruptive scenarios, and stringent quality management, crucial for meeting customer expectations, are fundamental in modern supply chains. The study emphasizes the importance of a risk management culture and the role of digital technologies in enhancing supply chain resilience, underscoring their vital contributions during crises like the COVID-19 pandemic. Thus, this work offers valuable insights into achieving more resilient and high-quality supply chains in today's challenging business environment through the integration of Quality Management Tools

Application of tuned mass damper for vibration control of frame structures under seismic excitations

Current trends in construction industry demands taller and lighter structures, which are also more flexible and having quite low damping value. This increases failure possibilities and also problems from serviceability point of view. Now-a-days several techniques are available to minimize the vibration of the structure, out of the several techniques available for vibration control ,concept of using TMD is a newer one. This study was made to study the effectiveness of using TMD for controlling vibration of structure. At first a numerical algorithm was developed to investigate the response of a shear building fitted with a TMD. Then another numerical algorithm was developed to investigate the response of a 2D frame model fitted with a TMD. A total of three loading conditions were applied at the base of the structure. First one was a sinusoidal loading, the second one was corresponding to compatible time history as per spectra of IS-1894 (Part -1):2002 for 5% damping at rocky soil with (PGA = 1g) and the third one was 1940 El Centro Earthquake record with (PGA = 0.313g). From the study it was found that, TMD can be effectively used for vibration control of structures. TMD was more effective when damping ratio of the structure is less. Gradually increasing the mass ratio of the TMD results in gradual decrement in the displacement response of the structure

IN VITRO CULTURE OF MOSS BRYUM CORONATUM SCHWAEGR.(BRYACEAE) AND IT'S PHYTOCHEMICAL ANALYSIS

Objective: The purpose of the present investigation was to establish in vitro conditions for moss Bryum coronatum Schwaegr. And to carry out preliminary phytochemical screening of B. coronatum leaf extracts in different solvents. Methods: Fresh unopened, mature capsules were used as explant and surface sterilization of spores bearing capsule with different concentration of sodium hypochlorite (0.5, 1, 2, 4 and 8%) with different time duration. The Mu­rashige and Skoog (MS) medium that contains different concentration(full,1/2, 1/4th, 1/8th strength) with different concentration of sucrose were used for culture this moss. Phytochemical screening were carried out using ethanol, methanol and ethyl acetate leaf extract of B. coronatum to identify various constitutes using the standard procedures. Results: Surface sterilization of spores of this moss was most effective in 4% commercial bleach for 1 min sterilization. The optimum condition for germination of spores and for proper growth of gametophytes B. coronatum on MS/4 medium strength with sucrose (1.5%), at pH 5.8 and temperature 22±2ºC with 16/8h: light/dark condition. Phytochemical analyses revealed the presence of alkaloids, terpenoids and saponins in all extracts. Conclusion: Four percentage NaOCl aqueous solutions are better for surface sterilization of moss sporophytes. MS/4 medium with 1.5% sucrose found the best medium for spore germination. Solvents extracts showed presence of alkaloids, terpenoids and saponins in all extracts

Modulation of p21, DAPK1 and COX-2 during the DMBA/TPA-induced mouse skin tumorigenesis and its prevention by phytic acid

Chemoprevention by naturally occurring agents is gaining much attention as a newer dimension in the management of cancer. Many naturally occurring agents have shown cancer chemopreventive potential in a variety of bioassay systems and animal models, having relevance to human disease. Phytic acid or Inositol hexaphosphate (IP6), an antioxidant, is a naturally occurring polyphosphorylated carbohydrate that has shown a strong anticancer activity in several experimental models. We assessed the protective effects of Phytic acid against the 7, 12-dimethylbenz [a] anthracene (DMBA)/ 12-O-tetradecanoylphorbol-13- acetate (TPA) induced mouse skin tumorigenesis at 4 and 16 weeks, the time before and after the tumor development. At molecular level we studied expression and promoter CpG methylation status of p21, DAPK1 and COX-2. Our data suggests exposure of DMBA/TPA methylated the promoter region of p21 and DAPK1 genes in time dependent manner that could be the cause of down regulation of their expression with time, which were reversed by administration of phytic acid. But we did not observe methylation in COX-2 whereas upregulation of COX-2 was observed at protein level in mice treated with DMBA followed by TPA in time dependent manner. Administration of phytic acid prevented theses DMBA/TPA induced molecular changes. Study provides a rationale for cancer chemoprevention by natural occurring compounds like Phytic acid

Effect of Kangaroo Mother Care on the breastfeeding, morbidity, and mortality of very low birth weight neonates: A prospective observational study

Objective: To compare the effect of Kangaroo Mother Care (KMC) versus conventional mother care (CMC) on growth, morbidity, mortality, and length of hospitalization in very low birth weight (VLBW) neonates. Study Design: A hospital based prospective observational study conducted in the pediatric department of a tertiary care teaching hospital in Southern Odisha. Materials and Methods: A total of 100 VLBW neonates were included. The effect of KMC on growth, morbidity, breastfeeding, and length of hospitalization was studied. The KMC group (n=50) was subjected to KMC for at least 6 h/day. The neonates received kangaroo care during hospitalization and at home. The control group (n=50) received CMC. Results: The KMC babies had better average weight gain per day (15.9±4.5 vs. 10.6±4.5 g, p<0.0001). The weekly increments in head circumference (0.75 vs. 0.49 cm, p=0.001), length (0.99 vs. 0.7 cm, p=0.021), and chest circumference (0.73 vs. 0.45 cm, p=0.004) were higher in the KMC group. Significantly more neonates receiving CMC suffered from hypothermia (36% vs. 6%), apnea (16% vs. 2%), and other minor illnesses (44% vs. 16%) than those receiving KMC. There was earlier hospital discharge in KMC group (6 vs. 18 days). More neonates of KMC group were exclusively breastfed at the end of the study (86% vs. 42%). No mortality was noted in either group. Conclusions: KMC improves growth, reduces morbidities, improves breastfeeding rates, and reduces hospitalization in VLBW neonates