INTEGRATED APPROACH OF SCHEDULING A FLEXIBLE JOB SHOP USING ENHANCED FIREFLY AND HYBRID FLOWER POLLINATION ALGORITHMS

Manufacturing industries are undergoing tremendous transformation due to Industry 4.0. Flexibility, consumer demands, product customization, high product quality, and reduced delivery times are mandatory for the survival of a manufacturing plant, for which scheduling plays a major role. A job shop problem modified with flexibility is called flexible job shop scheduling. It is an integral part of smart manufacturing. This study aims to optimize scheduling using an integrated approach, where assigning machines and their routing are concurrently performed. Two hybrid methods have been proposed: 1) The Hybrid Adaptive Firefly Algorithm (HAdFA) and 2) Hybrid Flower Pollination Algorithm (HFPA). To address the premature convergence problem inherent in the classic firefly algorithm, the proposed HAdFA employs two novel adaptive strategies: employing an adaptive randomization parameter (α), which dynamically modifies at each step, and Gray relational analysis updates firefly at each step, thereby maintaining a balance between diversification and intensification. HFPA is inspired by the pollination strategy of flowers. Additionally, both HAdFA and HFPA are incorporated with a local search technique of enhanced simulated annealing to accelerate the algorithm and prevent local optima entrapment. Tests on standard benchmark cases have been performed to demonstrate the proposed algorithm’s efficacy. The proposed HAdFA surpasses the performance of the HFPA and other metaheuristics found in the literature. A case study was conducted to further authenticate the efficiency of our algorithm. Our algorithm significantly improves convergence speed and enables the exploration of a large number of rich optimal solutions.

Beta-Lactamases in a Tertiary Care Hospital: "Biological Quake" Knocking at the Door

Background: Antimicrobial resistance due to the production of extended-spectrum β-lactamases (ESBLs), AmpC β-lactamases, and metallo-β-lactamases (MBLs) have emerged as a major health catastrophe limiting antibiotic treatment options. Therefore, this study was conducted to assess the current level of ESBLs, AmpC β-lactamases, and MBLs- producing bacteria and their antibiotic susceptibility profile in a Nepalese hospital. Methods: This was a cross-sectional study carried out among the inpatients of Medicare National Hospital, Kathmandu from April to September 2015. During the study period, a total of 589 specimens (urine, sputum, blood, pus, body fluids, throat swab, central venous catheter - CVC tip) collected aseptically from the admitted patients were selected in the study. The collected specimens were processed, and the isolated organisms were identified following the standard microbiological methods. ESBL was detected by standard combination disc method and double-disc synergy test.  Results: Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecalis, and Candida albicans were the common microbial agents isolated from hospital-admitted patients. Among total 84 Gram-negative bacteria tested for ESBL-production, 23 (27.4%) isolates were ESBL-producers. ESBL production was seen in 32.3% of Escherichia coli and 28.6% of Klebsiella pneumoniae isolates. Similarly, MBL production was identified in 28.6% of Pseudomonas aeruginosa, and 6.5% of Escherichia coli. Likewise, 3.2% of Escherichia coli were AmpC β-lactamase-producers. The ESBL-producing bacteria showed less susceptibility to different antibiotics as compared to non-ESBL-producers. Consistent results were found with different methods like combination disk method, MASTDISKSTM ID AmpC and ESBL disk, Ezy MICTM Strip (MIX+/MIX) method, and triple ESBL detection Ezy MICTM strips employed for the detection of ESBL and AmpC. Conclusions: ESBL was commonly seen in Escherichia coli while MBL in Pseudomonas aeruginosa. Routine monitoring of these kinds of resistance phenotypes following appropriate methods is essential for the proper treatment of patients. Keywords: Antibiotic resistance, Beta-lactamases, ESBL, Hospital-admitted patients, MB

MiR-133a Mimic Alleviates T1DM-Induced Systolic Dysfunction in Akita: An MRI-Based Study

Diabetic cardiomyopathy is a leading cause of heart failure. Developing a novel therapeutic strategy for diabetic cardiomyopathy and characterizing animal models used for diabetes mellitus (DM) are important. Insulin 2 mutant (Ins2+/-) Akita is a spontaneous, genetic, mouse model for T1DM, which is relevant to humans. There are contrasting reports on systolic dysfunction and pathological remodeling (hypertrophy and fibrosis) in Akita heart. Here, we used magnetic resonance imaging (MRI) approach, a gold standard reference for evaluating cardiac function, to measure ejection fraction (indicator of systolic dysfunction) in Akita. Moreover, we performed Wheat Germ Agglutinin (WGA) and hematoxylin and Eosin stainings to determine cardiac hypertrophy, and Masson’s Trichrome and picrosirius red stainings to determine cardiac fibrosis in Akita. MiR-133a, an anti-hypertrophy and anti-fibrosis miRNA, is downregulated in Akita heart. We determined if miR-133a mimic treatment could mitigate systolic dysfunction and remodeling in Akita heart. Our MRI results revealed decreased ejection fraction in Akita as compared to WT and increased ejection fraction in miR-133a mimic-treated Akita. We also found that miR-133a mimic treatment mitigates T1DM-induced cardiac hypertrophy and fibrosis in Akita. We conclude that Akita shows cardiac hypertrophy, fibrosis and systolic dysfunction and miR-133a mimic treatment to Akita could ameliorate them

A Review of Ergonomic Evaluation of Occupational Hazard of Indian Agriculture Farm and Allied Activities

Women in India are the major workforce in agriculture and perform almost all the agricultural activities. Women constitute 25.51% of the total work force in the country. The rural women play a vital role in agriculture and other agro based processing activities. They spent more time on post-harvest activities than those pre-harvest activities. Environmental and occupational health issues among agricultural workers expressed high levels of concern about working in hot weather, agricultural injuries, pesticides, awkward posture and drudgery prone activities. User Eco-friendly tools can increase the working efficiency and reduce the working load health hazards on farm activities during agricultural activities

Structural, morphological, optical and electrical characterization of gahnite ferroan nano composite derived from fly ash silica and ZnO Mixture

The synthesis of a high value-added product, gahnite ferroan nano composite, from a mixture of fly ash silica and ZnO is a low-cost and non-expensive technique. The XRD pattern clearly reveals the synthesized product from fly ash after leaching is a product of high-purity gahnite ferroan composite. The grains are mostly cubical in shape. The optical band gap of powdered gahnite ferroan nano composite is 3.37 eV, which acts as a UV protector. However, the bulk sample shows that the 500 to 700 nm wavelength of visible light is absorbed, and UV light is allowed to pass through. So, the bulk sample acts as a band pass filter of UV light which can be used in many optical applications for conducting UV-irradiation activity. Dielectric permittivity and dielectric loss increase with a rise in temperature. The increase in the ac conductivity at higher temperatures denotes the negative temperature coefficient resistance (NTCR) behavior of the material

Wastewater surveillance of antibiotic-resistant bacterial pathogens : A systematic review

Publisher Copyright: 2022, Tiwari, Kurittu, Al-Mustapha, Heljanko, Johansson, Thakali, Mishra, Lehto, Lipponen, Oikarinen, Pitkänen, WastPan Study Group and Heikinheimo.Infectious diseases caused by antibiotic-resistant bacterial (ARB) pathogens are a serious threat to human and animal health. The active surveillance of ARB using an integrated one-health approach can help to reduce the emergence and spread of ARB, reduce the associated economic impact, and guide antimicrobial stewardship programs. Wastewater surveillance (WWS) of ARB provides composite samples for a total population, with easy access to the mixed community microbiome. This concept is emerging rapidly, but the clinical utility, sensitivity, and uniformity of WWS of ARB remain poorly understood especially in relation to clinical evidence in sewershed communities. Here, we systematically searched the literature to identify studies that have compared findings from WWS of ARB and antibiotic resistance genes (ARG) with clinical evidence in parallel, thereby evaluating how likely WWS of ARB and ARG can relate to the clinical cases in communities. Initially, 2,235 articles were obtained using the primary search keywords, and 1,219 articles remained after de-duplication. Among these, 35 articles fulfilled the search criteria, and an additional 13 relevant articles were included by searching references in the primary literature. Among the 48 included papers, 34 studies used a culture-based method, followed by 11 metagenomics, and three PCR-based methods. A total of 28 out of 48 included studies were conducted at the single sewershed level, eight studies involved several countries, seven studies were conducted at national or regional scales, and five at hospital levels. Our review revealed that the performance of WWS of ARB pathogens has been evaluated more frequently for Escherichia coli, Enterococcus spp., and other members of the family Enterobacteriaceae, but has not been uniformly tested for all ARB pathogens. Many wastewater-based ARB studies comparing the findings with clinical evidence were conducted to evaluate the public health risk but not to relate with clinical evidence and to evaluate the performance of WWS of ARB. Indeed, relating WWS of ARB with clinical evidence in a sewershed is not straightforward, as the source of ARB in wastewater cannot be only from symptomatic human individuals but can also be from asymptomatic carriers as well as from animal sources. Further, the varying fates of each bacterial species and ARG within the sewerage make the aim of connecting WWS of ARB with clinical evidence more complicated. Therefore, future studies evaluating the performance of many AMR pathogens and their genes for WWS one by one can make the process simpler and the interpretation of results easier.Peer reviewe

Phenotypic Characterization of Multidrug-resistant Escherichia Coli with Special Reference to Extended-spectrum-beta-lactamases and Metallo-beta-lactamases in a Tertiary Care Center

Introduction: The increasing reports on extended-spectrum-beta-lactamase and metallo-betalactamase producing Escherichia coli have addressed a potential threat to global health since it is found to be highly resistance to most of the currently available antibiotics including carbapenems. The present study was aimed to determine the antibiogram of extended-spectrum-beta-lactamase and metallo-beta-lactamase producing MDR E. coli isolates from various clinical samples. Methods: This was a cross-sectional study conducted over a period of seven months (December 2013 to July 2014) at bacteriology laboratory of Tribhuvan University Teaching Hospital. A total of 250 clinical specimens (urine, pus, sputum, blood, body fluid, bile, tissue and central venous pressure line tip) were processed from inpatients, with multidrug-resistant Escherichia coli infections. Standard microbiological techniques were used for isolation and identification of the isolates. The presence of extended-spectrum-beta-lactamase was detected by phenotypic confirmatory test recommended by Clinical and Laboratory Standards Institute and imipenem (IMP) /EDTA combined disc method was performed to detect metallo-beta-lactamase mediated resistance mechanism. Results: We found high level of beta lactamase mediated resistance mechanism as part of multidrug resistance. Among 250 MDR isolates, 60% isolates were extended-spectrum-beta-lactamase producers and 17.2% isolates were metallo-beta-lactamase producers. Co-existence of extended-spectrum-betalactamase and metallo-beta-lactamase identified in 6.8% isolates. Conclusions: Beta-lactamase mediated resistance mechanisms are accounting very high in the multidrug resistant isolates of E. coli. Therefore, early detection of beta lactamase mediated resistant strains and their current antibiotic susceptibility pattern is necessary to avoid treatment failure and prevent the spread of MDR.  Keywords: e. coli; extended-spectrum-β-lactamase; metallo-β-lactamase; multidrug-resistance

Wastewater surveillance of antibiotic-resistant bacterial pathogens : A systematic review

Infectious diseases caused by antibiotic-resistant bacterial (ARB) pathogens are a serious threat to human and animal health. The active surveillance of ARB using an integrated one-health approach can help to reduce the emergence and spread of ARB, reduce the associated economic impact, and guide antimicrobial stewardship programs. Wastewater surveillance (WWS) of ARB provides composite samples for a total population, with easy access to the mixed community microbiome. This concept is emerging rapidly, but the clinical utility, sensitivity, and uniformity of WWS of ARB remain poorly understood especially in relation to clinical evidence in sewershed communities. Here, we systematically searched the literature to identify studies that have compared findings from WWS of ARB and antibiotic resistance genes (ARG) with clinical evidence in parallel, thereby evaluating how likely WWS of ARB and ARG can relate to the clinical cases in communities. Initially, 2,235 articles were obtained using the primary search keywords, and 1,219 articles remained after de-duplication. Among these, 35 articles fulfilled the search criteria, and an additional 13 relevant articles were included by searching references in the primary literature. Among the 48 included papers, 34 studies used a culture-based method, followed by 11 metagenomics, and three PCR-based methods. A total of 28 out of 48 included studies were conducted at the single sewershed level, eight studies involved several countries, seven studies were conducted at national or regional scales, and five at hospital levels. Our review revealed that the performance of WWS of ARB pathogens has been evaluated more frequently for Escherichia coli, Enterococcus spp., and other members of the family Enterobacteriaceae, but has not been uniformly tested for all ARB pathogens. Many wastewater-based ARB studies comparing the findings with clinical evidence were conducted to evaluate the public health risk but not to relate with clinical evidence and to evaluate the performance of WWS of ARB. Indeed, relating WWS of ARB with clinical evidence in a sewershed is not straightforward, as the source of ARB in wastewater cannot be only from symptomatic human individuals but can also be from asymptomatic carriers as well as from animal sources. Further, the varying fates of each bacterial species and ARG within the sewerage make the aim of connecting WWS of ARB with clinical evidence more complicated. Therefore, future studies evaluating the performance of many AMR pathogens and their genes for WWS one by one can make the process simpler and the interpretation of results easier.publishedVersionPeer reviewe