The timescale of early land plant evolution

Establishing the timescale of early land plant evolution is essential for testing hypotheses on the coevolution of land plants and Earth's System. The sparseness of early land plant megafossils and stratigraphic controls on their distribution make the fossil record an unreliable guide, leaving only the molecular clock. However, the application of molecular clock methodology is challenged by the current impasse in attempts to resolve the evolutionary relationships among the living bryophytes and tracheophytes. Here, we establish a timescale for early land plant evolution that integrates over topological uncertainty by exploring the impact of competing hypotheses on bryophyte-tracheophyte relationships, among other variables, on divergence time estimation. We codify 37 fossil calibrations for Viridiplantae following best practice. We apply these calibrations in a Bayesian relaxed molecular clock analysis of a phylogenomic dataset encompassing the diversity of Embryophyta and their relatives within Viridiplantae. Topology and dataset sizes have little impact on age estimates, with greater differences among alternative clock models and calibration strategies. For all analyses, a Cambrian origin of Embryophyta is recovered with highest probability. The estimated ages for crown tracheophytes range from Late Ordovician to late Silurian. This timescale implies an early establishment of terrestrial ecosystems by land plants that is in close accord with recent estimates for the origin of terrestrial animal lineages. Biogeochemical models that are constrained by the fossil record of early land plants, or attempt to explain their impact, must consider the implications of a much earlier, middle Cambrian-Early Ordovician, origin

Präferenz für Intuition und Deliberation – Messung und Konsequenzen von affekt- und kognitionsbasiertem Entscheiden

Personen unterscheiden sich darin, ob sie bevorzugt intuitiv oder reflektiv entscheiden (Epstein et al., 1996). Um diese individuellen Unterschiede zu erfassen, wurde ein neues Fragebogenmaß konstruiert, das die Präferenz für Intuition und Deliberation (PID, Betsch, 2004) auf zwei unabhängigen Skalen misst (Präferenz für Intuition und Präferenz für Deliberation). Intuition wird hier als ein rein affektiver Modus verstanden. Deliberation ist konzipiert als reflektiver, kognitionsbasierter Modus. In drei Studien wird die Konstruktion, Überprüfung der Zweidimensionalität und Validierung der Skala anhand von insgesamt über 2500 Versuchspersonen berichtet (Betsch, 2004). Präferenz für Intuition korreliert positiv mit schnellem Entscheiden, Extraversion und Verträglichkeit und ist unabhängig von der Fähigkeit zu logischem Denken. Präferenz für Deliberation korreliert mit Gewissenhaftigkeit, Perfektionismus, Bedürfnis nach Strukturiertheit und ist ebenfalls unabhängig von logischem Denken. Die Validität der Skala und die Implikationen für die Entscheidungsforschung werden in einer weiteren Studie überprüft, die die Krümmung der Nutzenfunktion mit der individuellen Präferenz für Intuition und Deliberation in Verbindung setzt (Schunk & Betsch, im Druck). Die Ergebnisse der Studie zeigen, dass die Entscheidungen intuitiver Menschen das Gefühl, das durch erlebtes Risiko evoziert wurde, integrieren, während dies bei deliberaten Personen nicht der Fall ist. Dies führt zu unterschiedlich gekrümmten Nutzenfunktionen. Neben den Haupteffekten der Strategiepräferenzen werden auch die Interaktion zwischen der individuell bevorzugten und der tatsächlich angewandten Strategie in fünf Studien untersucht (Betsch & Kunz, zur Veröffentlichung eingereicht). Die Ergebnisse der Studien zeigen, dass die Passung zwischen der bevorzugten und tatsächlich verwendeten Strategie (sogenannter decisional fit) den wahrgenommenen Wert des gewählten oder evaluierten Objektes erhöht und dass die Passung als Schutzschild dient gegenüber negativen Emotionen (z.B. Bedauern) nach Entscheidungen mit schlechtem Ausgang. Zusammenfassend umfasst diese Dissertation die Konstruktion und Validierung der Skala zur Erfassung der individuellen Präferenz für Intuition und Deliberation (PID; Betsch, 2004). Weiterhin untersucht sie die Konsequenzen von individuellen Unterschieden in affekt- vs. kognitionsbasiertem Entscheiden auf einen basalen Entscheidungsparameter (die Nutzenfunktion, Schunk & Betsch, im Druck). Außerdem zeigen weitere Studien die Konsequenzen einer Person x Situation Interaktion für zentrale Variablen der Entscheidungsliteratur auf (Wert, Bedauern; Betsch & Kunz, zur Veröffentlichung eingereicht)

Exploring Difference or Just Watching the Experts at Work? Interrogating Patient and Public Involvement (PPI) in a Cancer Research Setting Using the Work of Jurgen Habermas

Patient and public involvement (PPI) has emerged as a key consideration for organisations delivering health research and has spawned a burgeoning literature in the health and social sciences. The literature makes clear that PPI in health research encompasses a heterogeneous set of practices with levels of participation and involvement ranging from relatively minimal contributions to research processes to actively driving the research agenda. In this paper, we draw on the work of Jurgen Habermas to explore the ways in which PPI was accomplished in a cancer research setting in England. Drawing on ethnographic data with PPI participants and professional researchers, we describe the ways in which the life-world experiences of PPI participants were shaped by the health research system. We argue that PPI in this setting is less about exploring differences with regard to a plurality of expertise and more about simply watching or supporting the professional researchers at work

The Woody Guthrie Centennial Bibliography

This bibliography updates two extensive works designed to include comprehensively all significant works by and about Woody Guthrie. Richard A. Reuss published A Woody Guthrie Bibliography, 1912–1967 in 1968 and Jeffrey N. Gatten\u27s article “Woody Guthrie: A Bibliographic Update, 1968–1986” appeared in 1988. With this current article, researchers need only utilize these three bibliographies to identify all English-language items of relevance related to, or written by, Guthrie

Giving an Account of One’s Pain in the Anthropological Interview

In this paper, I analyze the illness stories narrated by a mother and her 13-year-old son as part of an ethnographic study of child chronic pain sufferers and their families. In examining some of the moral, relational and communicative challenges of giving an account of one’s pain, I focus on what is left out of some accounts of illness and suffering and explore some possible reasons for these elisions. Drawing on recent work by Judith Butler (Giving an Account of Oneself, 2005), I investigate how the pragmatic context of interviews can introduce a form of symbolic violence to narrative accounts. Specifically, I use the term “genre of complaint” to highlight how anthropological research interviews in biomedical settings invoke certain typified forms of suffering that call for the rectification of perceived injustices. Interview narratives articulated in the genre of complaint privilege specific types of pain and suffering and cast others into the background. Giving an account of one’s pain is thus a strategic and selective process, creating interruptions and silences as much as moments of clarity. Therefore, I argue that medical anthropologists ought to attend more closely to the institutional structures and relations that shape the production of illness narratives in interview encounters

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Phylogenetics of Seed Plants: An Analysis of Nucleotide Sequences from the Plastid Gene rbcL

We present the results of two exploratory parsimony analyses of DNA sequences from 475 and 499 species of seed plants, respectively, representing all major taxonomic groups. The data are exclusively from the chloroplast gene rbcL, which codes for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO or RuBPCase). We used two different state-transformation assumptions resulting in two sets of cladograms: (i) equal-weighting for the 499-taxon analysis; and (ii) a procedure that differentially weights transversions over transitions within characters and codon positions among characters for the 475-taxon analysis. The degree of congruence between these results and other molecular, as well as morphological, cladistic studies indicates that rbcL sequence variation contains historical evidence appropriate for phylogenetic analysis at this taxonomic level of sampling. Because the topologies presented are necessarily approximate and cannot be evaluated adequately for internal support, these results should be assessed from the perspective of their predictive value and used to direct future studies, both molecular and morphological. In both analyses, the three genera of Gnetales are placed together as the sister group of the flowering plants, and the anomalous aquatic Ceratophyllum (Ceratophyllaceae) is sister to all other flowering plants. Several major lineages identified correspond well with at least some recent taxonomic schemes for angiosperms, particularly those of Dahlgren and Thorne. The basalmost clades within the angiosperms are orders of the apparently polyphyletic subclass Magnoliidae sensu Cronquist. The most conspicuous feature of the topology is that the major division is not monocot versus dicot, but rather one correlated with general pollen type: uniaperturate versus triaperturate. The Dilleniidae and Hamamelidae are the only subclasses that are grossly polyphyletic; an examination of the latter is presented as an example of the use of these broad analyses to focus more restricted studies. A broadly circumscribed Rosidae is paraphyletic to Asteridae and Dilleniidae. Subclass Caryophyllidae is monophyletic and derived from within Rosidae in the 475-taxon analysis but is sister to a group composed of broadly delineated Asteridae and Rosidae in the 499-taxon study