The effects of dried tomato pomace and a multipleenzyme mixture supplementation (Rovabio ExcelTM) on performance and carcass quality of broiler chickens

An experiment was conducted to study the effect of dried tomato pomace (DTP) and a multienzyme preparation supplementation (Rovabio ExcelTM) on the performance and carcass quality of broiler chickens. A 4 × 2 factorial arrangement of treatments comprising 4 dietary DTP levels (0, 8, 16 and 24%) with and without enzyme supplementation was used. Each diet was fed to 4 replicates pens of 8 broiler chicks from 1 to 42 days posthatch as a coarse mash. The addition of DTP up to 16% to broiler diets did not significantly affect feed intake (FI), body weight gain (BWG) and feed conversion ratio (FCR) during starter (1 to 21 days), growing (22 to 42 days) and entire experimental (1 to 42 days) periods. Inclusion of 24% DTP into the diet significantly decreased FI at starter and entire experimental periods. Also, inclusion of 24% DTP into the diets significantly decreased BWG and increased FCR at all periods. Birds fed the diets supplemented with Rovabio ExcelTM had significantly higher BWG during the starter and entire experimental periods. No significant effect of enzyme addition was observed on FI and FCR. The dietary treatments with or without enzyme did not significantly affect mortality. The 8% DTP-fed group had comparable carcass weight, carcass yield, yield of breast, thigh, abdominal fat, heart, liver and gizzard with those of control group. The higher levels of DTP significantly decreased carcass weight and yield, yield of breast and thigh when compared to the control group. Enzyme supplementation significantly increased carcass weight and yield of abdominal fat. No significant interactions between the DTP and enzyme were observed for performance parameters and carcass characteristics. These results demonstrate that supplementation of DTP up to 8% to broiler diets had no significant adverse effects on performance parameters and carcass characteristics. The enzyme had a significant effect on BWG, carcass weight and yield of abdominal fat.Key words: Dried tomato pomace, enzyme, performance, carcass quality, broiler

Allele and genotype frequencies of -lactoglobulin gene in Iranian Najdi cattle and buffalo populations using PCR-RFLP

In modern programmes of animal breeding, the polymorphism of the milk proteins can be used as marker systems.  -Lactoglobulin is the major milk whey protein in the ruminants. Studies have indicated that this protein is polymorphic in the many breeds of cattle. This is the result of a single base pair substitution in the -lactoglobulin gene that also give rise to the Hae III restriction fragment length polymorphism. The aim of this work was to analyze the genotype distribution of -lactoglobulin in Iranian Najdi cattle and buffalo. Blood samples were supplied from 80 Najdi cattle and 80 buffalo from different cities of Khouzestan province. Polymerase chain reaction was applied to amplification of a 247 bp fragment of exon and intron IV of bovine -lactoglobulin gene. The Hae III enzyme was used for restriction of the PCR products. The digested products were separated by electrophoresis on 2.5% agarose gel. The allele B of -Lactoglobulin occurred at a higher frequency than the allele A in both Najdi cattle and buffalo. The genotype frequencies of AA, AB, and BB in Najdi cattle and buffalo were 0, 0.175, 0.825 and 0.04, 0.3, 0.66 respectively. Frequencies of A and B alleles were 0.0875 and 0.9125, and 0.1875 and 0.8125 in Najdi cattle and buffalo, respectively. The deviation from Hardy-Weinberg equilibrium was not detected in any of those animals. Detection of heterozygosity indicator evidence on low level of heterozygosity and genetic variability in both population

On the Use of Hydrodynamic Instability Test as an Efficient Tool for Evaluating Viscoelastic Fluid Models

Abstract The so-called ''second-order'' rheological model has been of widespread use in studies related to viscoelastic fluids. The model, however, has been shown by Mathematics Subject Classification: 76E2

Nuclear medicine program progress report for quarter ending March 31, 1995

In this report the conditions for ``direct`` labeling of the anti-granulocyte (MAb) BW 250/183 monoclonal antibody with rhenium-188 (Re-188) from a generator are described. Re-188-BW 250/183 is of interest for potential use for bone marrow ablation. The labeling time, temperature, pH, and the amount of tin and citric acid were optimized utilizing IgG. Radiolabeling yields of greater than 97% were achieved using 1 mL of a phthalate/tartrate buffer (pH 5.{und M}=?), 250 {micro} g BW 250/183, 1.0 mg citric acid, 400 {micro} g tin (II) chloride, and 1 mL of the tungsten-188/rhenium-188 generator eluent (200--800 {micro} Ci of Re-188). Analysis of the Re-188-labeled IgG and BW 250/183 was performed by Instant Thin Layer Chromatography (ITLC), Sephadex purification and High Performance Liquid Chromatography (HPLC). When the labeling was performed at room temperature or 37 C, in vitro stability studies performed in HSA solution, cysteine solution, 6 {und M} urea solution and a 1% casein solution showed that the Re-188 label demonstrated a similar stability profile in all solutions. Initial studies indicate that Re-188-BW 250/183 retained {approximately} 90% of immunoreactivity when compared to the technetium-99m labeled antibody prepared from the same kit. During this period, several radioisotopes prepared in the ORNL HFIR were also supplied on a cost-recovery basis or provided to collaborators for ongoing collaborative projects. These include tin-117m, processed tungsten-188 and the ORNL alumina-based tungsten-188/rhenium-188 generators

Nuclear medicine program progress report for quarter ending September 30, 1996

The reactor production yields of tungsten-188 produced by neutron capture by enriched tungsten-186 in the HFIR and other reactors are nearly an order of magnitude lower than expected by calculation using established cross section values. Since neutron capture of tungsten-188 may be the major factor which significantly reduces the observed yields of tungsten-188, the authors have evaluated the possible burn-up cross section of the tungsten-188 product. Tungsten-189 was produced by irradiating a radioactive target containing a known amount of {sup 188}W. In order to reduce the radiation level to an acceptable level (90% of {sup 188}Re, which is the decay product of {sup 188}W, prior to irradiation. They were able to confirm the two predominant {gamma}-rays in the decay of {sup 189}W, 260.1 {+-} 1.4 and 421.5 {+-} 1.6 keV. By following the decay of these {gamma}-rays in two sets of experiments, a half-life of 10.8 {+-} 0.3 m was obtained for {sup 189}W. Based on a knowledge of the {sup 188}W content of target (52.6 mBq), neutron flux of 5 {times} 10{sup 13} n {center_dot} s{sup {minus}1} {center_dot} cm{sup {minus}2}, irradiation time of 10 min and with the assumption of 100% intensity for 260.1 and 421.5 keV {gamma}-rays, a cross-section of 12.0 {+-} 2.5 b was calculated for burn-up cross-section of {sup 188}W, which helps explain the greatly reduced production yields of {sup 188}W

Nuclear Medicine Program progress report for quarter ending September 30, 1992

The radioiodination and in vivo evaluation of p-iodocaramiphen a muscarinic antagonist which binds with high affinity to the M{sub 1} receptor subtype in vitro are described. Biodistribution studies in female Fischer rats demonstrated that [{sup 125}I]-piodocaraminphen had significant cerebral localization, but the uptake did not demonstrate specific uptake in those cerebral regions rich in muscarinic receptors, and radioactivity washed out rapidly from the brain. In addition there was no significant blockage of activity when the rats were preinjected with quinuclidinyl benzilate. These results suggest that p-iodocaramiphen is not a good candidate for the in vivo study of M{sub 1} muscarinic receptor populations by SPECT. Because of the widespread interest and expected importance of the availability of large amounts of tungsten-188 required for the tungsten-188/rhenium-188 generator systems, we have investigated the large-scale production of tungsten-188 in the ORNL HFIR. We have also compared our production data with the theoretical production values and with experimental data available in the literature from other reactors. Tungsten-188 is produced in a fission nuclear reactor by double neutron capture of tungsten-186. The experimental yield of tungsten-188 is approximately 4 mCi/mg of tungsten-186 at the end of bombardment (EOB) in the HFIR operating at 85 MWt power for a one cycle irradiation ({approximately}21 days) at a thermal neutron flux of 2 {times} 10{sup 15} n.s{sup {minus}1}cm{sup {minus}2}

Monitoring of Cochlodinium sp for shrimp farms in Hormozgan Province

The Blooming due to the some species of phytoplanktons especially Dynoflagellates has made some problems for water ecosystems and aquaculture. In this study, the density of phytoplanktons specially Cochlodinium sp. and also environmental factors such as temperature, pH, dissolved Oxygen, and transparency were recorded two weekly in 18 stations of Hormozgan province, Iran in order to monitoring of the possibility of phytoplankton blooming. During six months monitoring, the target phytoplankton, Cochlodinium sp was not observed in shrimp farms. But, other phytoplanktons and zooplanktons were observed as follow: 13 genus of phytoplankton and six genuses of zooplanktons has found in ponds, main water channel and sea. The diatoms with 10 genuses had the highest abundance and Dynoflagellates with 3 genuses had the lowest abundance and blue-green phytoplankton with one genus was in lowest group. Totally, diatoms with 77%, Dynoflagellate with 15% and blue-green alga with 8% abundance were the main populations of planktons in the studied area

An EBSD study of the deformation of service-aged 316 austenitic steel

Electron backscatter diffraction (EBSD) has been used to examine the plastic deformation of an ex-service 316 austenitic stainless steel at 297K and 823K (24 °C and 550 °C)at strain rates 3.5x10-3 to 4 x 10-7 s-1. The distribution of local misorientations was found to depend on the imposed plastic strain following a lognormal distribution at true strains 0.1. At 823 K (550 °C), the distribution of misorientations depended on the applied strain rate. The evolution of lattice misorientations with increasing plastic strain up to 0.23 was quantified using the metrics kernel average misorientation, average intragrain misorientation, and low angle misorientation fraction. For strain rate down to 10-5 s-1 all metrics were insensitive to deformation temperature, mode (tension vs. compression) and orientation of the measurement plane. The strain sensitivity of the different metrics was found to depend on the misorientation ranges considered in their calculation. A simple new metric, proportion of undeformed grains, is proposed for assessing strain in both aged and unaged material. Lattice misorientations build up with strain faster in aged steel than in un-aged material and most of the metrics were sensitive to the effects of thermal aging. Ignoring aging effects leads to significant overestimation of the strains around welds. The EBSD results were compared with nanohardness measurements and good agreement established between the two techniques of assessing plastic strain in aged 316 steel

The Probable Cell of Origin of NF1- and PDGF-Driven Glioblastomas

Primary glioblastomas are subdivided into several molecular subtypes. There is an ongoing debate over the cell of origin for these tumor types where some suggest a progenitor while others argue for a stem cell origin. Even within the same molecular subgroup, and using lineage tracing in mouse models, different groups have reached different conclusions. We addressed this problem from a combined mathematical modeling and experimental standpoint. We designed a novel mathematical framework to identify the most likely cells of origin of two glioma subtypes. Our mathematical model of the unperturbed in vivo system predicts that if a genetic event contributing to tumor initiation imparts symmetric self-renewing cell division (such as PDGF overexpression), then the cell of origin is a transit amplifier. Otherwise, the initiating mutations arise in stem cells. The mathematical framework was validated with the RCAS/tv-a system of somatic gene transfer in mice. We demonstrated that PDGF-induced gliomas can be derived from GFAP-expressing cells of the subventricular zone or the cortex (reactive astrocytes), thus validating the predictions of our mathematical model. This interdisciplinary approach allowed us to determine the likelihood that individual cell types serve as the cells of origin of gliomas in an unperturbed system