Combination of carbon nanotubes and two-photon absorbers for broadband optical limiting

New systems are required for optical limiting against broadband laser pulses. We demonstrate that the association of non-linear scattering from single-wall carbon nanotubes (SWNT) and multiphoton absorption (MPA) from organic chromophores is a promising approach to extend performances of optical limiters over broad spectral and temporal ranges. Such composites display high linear transmission and good neutral colorimetry and are particularly efficient in the nanosecond regime due to cumulative effects.Comment: 5 avril 200

The interaction between lipid derivatives of colchicine and tubulin: Consequences of the interaction of the alkaloid with lipid membranes

AbstractColchicine is a potent antimitotic poison which is well known to prevent microtubule assembly by binding tubulin very tightly. Colchicine also possesses anti-inflammatory properties which are not well understood yet. Here we show that colchicine tightly interacts with lipid layers. The physical and biological properties of three different lipid derivatives of colchicine are investigated parallel to those of membrane lipids in the presence of colchicine. Upon insertion in the fatty alkyl chains, colchicine rigidifies the lipid monolayers in a fluid phase and fluidifies rigid monolayers. Similarly X-ray diffraction data show that lecithin–water phases are destabilized by colchicine. In addition, an unexpectedly drastic enhancement of the photoisomerization rate of colchicine into lumicolchicine in the lipid environment is observed and further supports insertion of the alkaloid in membranes. Finally the interaction of colchicine with lipids makes the drug inaccessible to tubulin. The possible in vivo significance of these results is discussed

Design of Functionalized Lipids and Evidence for Their Binding to Photosystem II Core Complex by Oxygen Evolution Measurements, Atomic Force Microscopy, and Scanning Near-Field Optical Microscopy

AbstractPhotosystem II core complex (PSII CC) absorbs light energy and triggers a series of electron transfer reactions by oxidizing water while producing molecular oxygen. Synthetic lipids with different alkyl chains and spacer lengths bearing functionalized headgroups were specifically designed to bind the QB site and to anchor this large photosynthetic complex (240 kDa) in order to attempt two-dimensional crystallization. Among the series of different compounds that have been tested, oxygen evolution measurements have shown that dichlorophenyl urea (DCPU) binds very efficiently to the QB site of PSII CC, and therefore, that moiety has been linked covalently to the headgroup of synthetic lipids. The analysis of the monolayer behavior of these DCPU-lipids has allowed us to select ones bearing long spacers for the anchoring of PSII CC. Oxygen evolution measurements demonstrated that these long-spacer DCPU-lipids specifically bind to PSII CC and inhibit electron transfer. With the use of atomic force microscopy (AFM) and scanning near-field optical microscopy (SNOM), it was possible to visualize domains of PSII CC bound to DCPU-lipid monolayers. SNOM imaging has enabled us to confirm that domains observed by AFM were composed of PSII CC. Indeed, the SNOM topography images presented similar domains as those observed by AFM, but in addition, it allowed us to determine that these domains are fluorescent. Electron microscopy of these domains, however, has shown that the bound PSII CC was not crystalline

Broadband optical limiting optimisation by combination of carbon nanotubes and two-photon absorbing chromophores in liquids

International audienceWe report here on the optical limiting studies performed with nanosecond laser pulses on several families of multiphoton absorbers in chloroform, with carbon nanotubes suspended in solutions. Performances of these samples are compared with those of simple multiphoton absorber solutions and carbon nanotube suspensions, and the differences observed are interpreted in terms of cumulative NLO effects and adverse aggregation phenomenon