Reading a GEM with a VLSI pixel ASIC used as a direct charge collecting anode

In MicroPattern Gas Detectors (MPGD) when the pixel size is below 100 micron and the number of pixels is large (above 1000) it is virtually impossible to use the conventional PCB read-out approach to bring the signal charge from the individual pixel to the external electronics chain. For this reason a custom CMOS array of 2101 active pixels with 80 micron pitch, directly used as the charge collecting anode of a GEM amplifying structure, has been developed and built. Each charge collecting pad, hexagonally shaped, realized using the top metal layer of a deep submicron VLSI technology is individually connected to a full electronics chain (pre-amplifier, shaping-amplifier, sample and hold, multiplexer) which is built immediately below it by using the remaining five active layers. The GEM and the drift electrode window are assembled directly over the chip so the ASIC itself becomes the pixelized anode of a MicroPattern Gas Detector. With this approach, for the first time, gas detectors have reached the level of integration and resolution typical of solid state pixel detectors. Results from the first tests of this new read-out concept are presented. An Astronomical X-Ray Polarimetry application is also discussed.Comment: 11 pages, 14 figures, presented at the Xth Vienna Conference on Instrumentation (Vienna, February 16-21 2004). For a higher resolution paper contact [email protected]

Single-shot X-ray phase-contrast computed tomography with non-microfocal laboratory sources

We present a method that enables performing x-ray phase-contrast imaging (XPCI) computed tomography with a laboratory setup using a single image per projection angle, eliminating the need to move optical elements during acquisition. Theoretical derivation of the method is presented, and its validity conditions are provided. The object is assumed to be quasihomogeneous, i.e., to feature a ratio between the refractive index and the linear attenuation coefficient that is approximately constant across the field of view. The method is experimentally demonstrated on a plastics phantom and on biological samples using a continuous rotation acquisition scheme achieving scan times of a few minutes. Moreover, we show that such acquisition times can be further reduced with the use of a high-efficiency photon-counting detector. Thanks to its ability to substantially simplify the image-acquisition procedure and greatly reduce collection times, we believe this method represents a very important step towards the application of XPCI to real-world problems

Spectral and polarimetric characterization of the Gas Pixel Detector filled with dimethyl ether

The Gas Pixel Detector belongs to the very limited class of gas detectors optimized for the measurement of X-ray polarization in the emission of astrophysical sources. The choice of the mixture in which X-ray photons are absorbed and photoelectrons propagate, deeply affects both the energy range of the instrument and its performance in terms of gain, track dimension and ultimately, polarimetric sensitivity. Here we present the characterization of the Gas Pixel Detector with a 1 cm thick cell filled with dimethyl ether (DME) at 0.79 atm, selected among other mixtures for the very low diffusion coefficient. Almost completely polarized and monochromatic photons were produced at the calibration facility built at INAF/IASF-Rome exploiting Bragg diffraction at nearly 45 degrees. For the first time ever, we measured the modulation factor and the spectral capabilities of the instrument at energies as low as 2.0 keV, but also at 2.6 keV, 3.7 keV, 4.0 keV, 5.2 keV and 7.8 keV. These measurements cover almost completely the energy range of the instrument and allows to compare the sensitivity achieved with that of the standard mixture, composed of helium and DME.Comment: 20 pages, 11 figures, 5 tables. Accepted for publication by NIM

Low energy polarization sensitivity of the Gas Pixel Detector

An X-ray photoelectric polarimeter based on the Gas Pixel Detector has been proposed to be included in many upcoming space missions to fill the gap of about 30 years from the first (and to date only) positive measurement of polarized X-ray emission from an astrophysical source. The estimated sensitivity of the current prototype peaks at an energy of about 3 keV, but the lack of readily available polarized sources in this energy range has prevented the measurement of detector polarimetric performances. In this paper we present the measurement of the Gas Pixel Detector polarimetric sensitivity at energies of a few keV and the new, light, compact and transportable polarized source that was devised and built to this aim. Polarized photons are produced, from unpolarized radiation generated with an X-ray tube, by means of Bragg diffraction at nearly 45 degrees. The employment of mosaic graphite and flat aluminum crystals allow the production of nearly completely polarized photons at 2.6, 3.7 and 5.2 keV from the diffraction of unpolarized continuum or line emission. The measured modulation factor of the Gas Pixel Detector at these energies is in good agreement with the estimates derived from a Monte Carlo software, which was up to now employed for driving the development of the instrument and for estimating its low energy sensitivity. In this paper we present the excellent polarimetric performance of the Gas Pixel Detector at energies where the peak sensitivity is expected. These measurements not only support our previous claims of high sensitivity but confirm the feasibility of astrophysical X-ray photoelectric polarimetry.Comment: 15 pages, 12 figures. Accepted for publication in NIM

XPOL-III: a New-Generation VLSI CMOS ASIC for High-Throughput X-ray Polarimetry

While the successful launch and operation in space of the Gas Pixel Detectors onboard the PolarLight cubesat and the Imaging X-ray Polarimetry Explorer demonstrate the viability and the technical soundness of this class of detectors for astronomical X-ray polarimetry, it is clear that the current state of the art is not ready to meet the challenges of the next generation of experiments, such as the enhanced X-ray Timing and Polarimetry mission, designed to allow for a significantly larger data throughput. In this paper we describe the design and test of a new custom, self-triggering readout ASIC, dubbed XPOL-III, specifically conceived to address and overcome these limitations. While building upon the overall architecture of the previous generations, the new chip improves over its predecessors in several, different key areas: the sensitivity of the trigger electronics, the flexibility in the definition of the readout window, as well as the maximum speed for the serial event readout. These design improvements, when combined, allow for almost an order of magnitude smaller dead time per event with no measurable degradation of the polarimetric, spectral, imaging or timing capability of the detector, providing a good match for the next generation of X-ray missions.Comment: accepted for publication at Nuclear Inst. and Methods in Physics Research Section

Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cows

The study investigated the effect of an intramammary lipopolysaccharide (LPS) challenge on the bovine mammary and liver transcriptome and its consequences on metabolic biomarkers and liver tissue composition. At 7 days of lactation, 7 cows served as controls (CTR) and 7 cows (LPS) received an intramammary Escherichia coli LPS challenge. The mammary and liver tissues for transcriptomic profiling were biopsied at 2.5 h from challenge. Liver composition was evaluated at 2.5 h and 7 days after challenge, and blood biomarkers were analyzed at 2, 3, 7 and 14 days from challenge. In mammary tissue, the LPS challenge resulted in 189 differentially expressed genes (DEG), with 20 down-regulated and 169 up-regulated. In liver tissue, there were 107 DEG in LPS compared with CTR with 42 down-regulated and 65 up-regulated. In mammary, bioinformatics analysis highlighted that LPS led to activation of NOD-like receptor signaling, Toll-like receptor signaling, RIG-I-like receptor signaling and apoptosis pathways. In liver, LPS resulted in an overall inhibition of fatty acid elongation in mitochondria and activation of the p53 signaling pathway. The LPS challenge induced changes in liver lipid composition, a systemic inflammation (rise of blood ceruloplasmin and bilirubin), and an increase in body fat mobilization. The data suggest that cells within the inflamed mammary gland respond by activating mechanisms of pathogen recognition. However, in the liver the response likely depends on mediators originating from the udder that affect liver functionality and specifically fatty acid metabolism (b-oxidation, ketogenesis, and lipoprotein synthesis).Fil: Minuti, Andrea. Universita Cattolica del Sacro Cuore; ItaliaFil: Zhou, Zheng. University Of Illinois At Urbana; Estados UnidosFil: Graugnard, Daniel E. University Of Illinois At Urbana; Estados UnidosFil: Rodriguez Zas, Sandra L.. University Of Illinois At Urbana; Estados UnidosFil: Palladino, Rafael Alejandro. Universidad de Buenos Aires. Facultad de Agronomia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cardoso, Felipe C.. University Of Illinois At Urbana; Estados UnidosFil: Trevisi, Erminio. Universita Cattolica del Sacro Cuore; ItaliaFil: Loor, Juan J. University Of Illinois At Urbana; Estados Unido

The imaging properties of the Gas Pixel Detector as a focal plane polarimeter

X-rays are particularly suited to probe the physics of extreme objects. However, despite the enormous improvements of X-ray Astronomy in imaging, spectroscopy and timing, polarimetry remains largely unexplored. We propose the photoelectric polarimeter Gas Pixel Detector (GPD) as an instrument candidate to fill the gap of more than thirty years of lack of measurements. The GPD, in the focus of a telescope, will increase the sensitivity of orders of magnitude. Moreover, since it can measure the energy, the position, the arrival time and the polarization angle of every single photon, allows to perform polarimetry of subsets of data singled out from the spectrum, the light curve or the image of source. The GPD has an intrinsic very fine imaging capability and in this work we report on the calibration campaign carried out in 2012 at the PANTER X-ray test facility of the Max-Planck-Institut f\"ur extraterrestrische Physik of Garching (Germany) in which, for the first time, we coupled it to a JET-X optics module with a focal length of 3.5 m and an angular resolution of 18 arcsec at 4.5 keV. This configuration was proposed in 2012 aboard the X-ray Imaging Polarimetry Explorer (XIPE) in response to the ESA call for a small mission. We derived the imaging and polarimetric performance for extended sources like Pulsar Wind Nebulae and Supernova Remnants as case studies for the XIPE configuration, discussing also possible improvements by coupling the detector with advanced optics, having finer angular resolution and larger effective area, to study with more details extended objects.Comment: Accepted for publication in The Astrophysical Journal Supplemen

Reliable protocol for sample preparation to observe nanomaterial adherence to the surface of biological cells by using scanning electron microscopy

Background. When assessing the interaction between nanomaterials and cells, an important step is to image the effects induced by the material to the cell membrane. In order to evaluate the results of the interaction process, scanning electron microscopy (SEM) is typically used. Commonly, an important step during the preparation of biological cell samples for SEM is represented by the critical point drying, which involves the replacement of the alcohol used for dehydration with an inert gas. This conventional drying method is not only hard to accomplish, but it can lead to sample destruction if specific parameters are not met. On the other hand, when assessing nanomaterials adhered to the cell membrane, the integrity of the cell is not necessarily important, so a little cell deflation doesn’t affect the intended purpose of the evaluation. Here, we describe a simple and more cost efficient method to prepare biological samples for SEM imaging that preserves cell integrity and can be used to describe nanomaterials interaction with cell surface. Methods. Cells were grown on sterilized silica chips, after which the evaluated nanomaterial was added to the cell culture media at least 24h for incubation. Afterwards, the samples were washed to eliminate non-adhered nanomaterials, fixed with glutaraldehyde and osmium tetroxide, and dehydrated with increasing concentrations of alcohol. The silica chips were then air dried in the biological safety hood and in vacuum, followed by a sputter coat film of 5 nm of gold. The samples were imaged with a scanning electron microscope. Results. We were able to obtain well preserved biological cell samples, both with and without nanomaterials adhered to the cell membrane surface. Nanomaterials such as magnetic nanoparticles and magnetic nanowires were easily traceable on cell surface. Furthermore, the nanomaterials were clearly observed in the images obtained, while the cell surface was not affected by the drying process applied. Although the samples obtained using our method were characterized by a slight deflation of the cells, the morphology of the cells is well preserved and the method is suitable for the evaluation of the interaction between nanomaterials and cell surfaces. Conclusions: We have described a novel cost efficient and easy to perform method for processing biological samples for SEM imaging that preserves cell morphology and can be used for analyzing nanoparticle and nanowires interaction with cell surface

Imaging with the invisible light

We describe a UV photo-detector with single photon(electron) counting and imaging capability. It is based on a CsI photocathode, a GEM charge multiplier and a self triggering CMOS analog pixel chip with 105k pixels at 50 micron pitch. The single photoelectron produced by the absorption of a UV photon is drifted to and multiplied inside a single GEM hole. The coordinates of the GEM avalanche are reconstructed with high accuracy (4 micron rms) by the pixel chip. As a result the map of the GEM holes, arranged on a triangular pattern at 50micron pitch, is finely imaged.Comment: 6 pages, 14 figures, presented at the 11th Vienna Conference on Instrumentation VIC 2007, submitted to Nuclear Instruments and Methods