Transient pressure analysis of a volume fracturing well in fractured tight oil reservoirs

This research was supported by the Ministry of Land and Resources Special Geological Survey: Upper Paleozoic Marine Shale Gas Geological Survey in Yunnan, Guizhou, Guangxi Region (DD20160178), The Key Laboratory of Unconventional Petroleum Geology of Geological Survey of China Open Fund and the Major National R&D Projects: Study on the Test Method for Shale Structure and Composition at Different Scales with project number: 2016ZX05034-003-006.Peer reviewedPostprin

Adaptive Müller cell responses to microglial activation mediate neuroprotection and coordinate inflammation in the retina

<p>Abstract</p> <p>Purpose</p> <p>Microglia and Müller cells are prominent participants in retinal responses to injury and disease that shape eventual tissue adaptation or damage. This investigation examined how microglia and Müller cells interact with each other following initial microglial activation.</p> <p>Methods</p> <p>Mouse Müller cells were cultured alone, or co-cultured with activated or unactivated retinal microglia, and their morphological, molecular, and functional responses were evaluated. Müller cell-feedback signaling to microglia was studied using Müller cell-conditioned media. Corroborative <it>in vivo </it>analyses of retinal microglia-Müller cell interactions in the mouse retina were also performed.</p> <p>Results</p> <p>Our results demonstrate that Müller cells exposed to activated microglia, relative to those cultured alone or with unactivated microglia, exhibit marked alterations in cell morphology and gene expression that differed from those seen in chronic gliosis. These Müller cells demonstrated <it>in vitro </it>(1) an upregulation of growth factors such as GDNF and LIF, and provide neuroprotection to photoreceptor cells, (2) increased pro-inflammatory factor production, which in turn increased microglial activation in a positive feedback loop, and (3) upregulated chemokine and adhesion protein expression, which allowed Müller cells to attract and adhere to microglia. <it>In vivo </it>activation of microglia by intravitreal injection of lipopolysaccharide (LPS) also induced increased Müller cell-microglia adhesion, indicating that activated microglia may translocate intraretinally in a radial direction using Müller cell processes as an adhesive scaffold.</p> <p>Conclusion</p> <p>Our findings demonstrate that activated microglia are able to influence Müller cells directly, and initiate a program of bidirectional microglia-Müller cell signaling that can mediate adaptive responses within the retina following injury. In the acute aftermath following initial microglia activation, Müller cell responses may serve to augment initial inflammatory responses across retinal lamina and to guide the intraretinal mobilization of migratory microglia using chemotactic cues and adhesive cell contacts. Understanding adaptive microglia-Müller cell interactions in injury responses can help discover therapeutic cellular targets for intervention in retinal disease.</p

Impaired Caveolae Function and Upregulation of Alternative Endocytic Pathways Induced by Experimental Modulation of Intersectin-1s Expression in Mouse Lung Endothelium

Intersectin-1s (ITSN-1s), a protein containing five SH3 (A-E) domains, regulates via the SH3A the function of dynamin-2 (dyn2) at the endocytic site. ITSN-1s expression was modulated in mouse lung endothelium by liposome delivery of either a plasmid cDNA encoding myc-SH3A or a specific siRNA targeting ITSN-1 gene. The lung vasculature of SH3A-transduced and ITSN-1s- deficient mice was perfused with gold albumin (Au-BSA) to analyze by electron microscopy the morphological intermediates and pathways involved in transendothelial transport or with dinitrophenylated (DNP)-BSA to quantify by ELISA its transport. Acute modulation of ITSN-1s expression decreased the number of caveolae, impaired their transport, and opened the interendothelial junctions, while upregulating compensatory nonconventional endocytic/transcytotic structures. Chronic inhibition of ITSN-1s further increased the occurrence of nonconventional intermediates and partially restored the junctional integrity. These findings indicate that ITSN-1s expression is required for caveolae function and efficient transendothelial transport. Moreover, our results demonstrate that ECs are highly adapted to perform their transport function while maintaining lung homeostasis

Interferon regulatory factor 7- (IRF7-) mediated immune response affects Newcastle disease virus replication in chicken embryo fibroblasts

Interferon regulatory factor 7 (IRF7) is essential for the induction of an antiviral response. Previous studies have shown that virus replication causes the activation or expression of Type I interferon (IFN) in cells, which further activates IFN-stimulated genes (ISGs) to retard virus growth. In this study, after infection of chicken embryo fibroblasts (CEFs) with the lentogenic Newcastle disease virus (NDV) strain LaSota or the velogenic NDV strain GM, the mRNA and protein levels of IRF7 showed a significant increase, and part of the IRF7 protein was translocated from the cytoplasm to the nucleus. In order to further explore the effect of IRF7-mediated innate immune response on the replication of NDV in CEFs, the mRNA levels of IFN-α, IFN-β and STAT1 were measured and the replication kinetics of NDV determined. The results showed that specific siRNA could inhibit the expression of IRF7 and limit the mRNA level of IFN-α, IFN-β and STAT1 and, accordingly, the replication kinetics of both NDVs were enhanced after the inhibition of IRF7. In conclusion, IRF7 is an important nuclear transcription factor for the induction of Type I IFNs during the antiviral response, which can affect the replication of NDV and spread to CEFs in the early phase of viral infection

Solar carbon fuel via photoelectrochemistry

A promising strategy to mitigate both energy shortage and global warming is the conversion of CO 2 into chemicals that can be used as fuels (chemical fuels) by utilizing renewable energy sources. Up to date, solar-driven CO 2 reduction has been achieved with photochemical (PC) and photoelectrochemical (PEC) systems or electrochemical cells combined with a photovoltaic system (PV-EC). This study is intended to compare and highlight the state-of-the-art PEC systems for CO 2 reduction and show the limitation factors that still hinder their widespread utilization. The review starts with a description of semiconducting photocatalyst properties and fundamental understanding of PEC CO 2 reduction process. Then, the most significant performance metrics used for evaluation of PEC systems are explained in details. In addition, recent progress in PEC CO 2 reduction systems is summarized and classified in different categories according to the chemical product. Different strategies such as doping, combination of two or more semiconductors, synthesis of nanostructured materials, passivation layers and co-catalysts that enhance light absorption, chemical stability, charge transfer and reduce ohmic losses and overpotentials of photoactive materials are reviewed. Besides the improvement of photocatalysts, research progress on the front of PEC reactor design, combined with the development of advanced modelling tools and characterization techniques are expected to bring PEC CO 2 reduction a step closer to commercialization

Detection and identification of NAP-2 as a biomarker in hepatitis B-related hepatocellular carcinoma by proteomic approach

<p>Abstract</p> <p>Background</p> <p><b>A </b>lack of sensitive and specific biomarkers is a major reason for the high rate of Primary hepatocellular carcinoma (HCC)-related mortality. The aim of this study was to investigate potential proteomic biomarkers specific for HCC.</p> <p>Methods</p> <p>81 patients with hepatitis B-related HCC and 33 healthy controls were randomly divided into a training set (33 HCC, 33 controls) and a testing set (48 HCC, 33 controls). Serum proteomic profiles were measured using Surface-enhanced laser desorption/ionization-time-of-flight mass spectroscopy (SELDI-TOF-MS).) A classification tree was established by Biomarker Pattern Software (BPS). Candidate SELDI peaks were isolated by tricine-SDS-PAGE, identified by HPLC-MS/MS and validated by immunohistochemistry (IHC) in liver tissues.</p> <p>Results</p> <p>A total of 6 proteomic peaks (3157.33 m/z, 4177.02 m/z, 4284.79 m/z, 4300.80 m/z, 7789.87 m/z, and 7984.14 m/z) were chosen by BPS to establish a classification tree with the highest discriminatory power in the training set. The sensitivity and specificity of this classification tree were 95.92%, and 100% respectively in the testing set. A candidate marker of about 7984 m/z was isolated and identified as neutrophil-activating peptide 2 (NAP-2). IHC staining showed that NAP-2 signals were positive in HCC tissues but negative in adjacent tissues.</p> <p>Conclusion</p> <p>The NAP-2 may be a specific proteomic biomarker of hepatitis B-related HCC.</p

Shear Wave Propagation in Soft Tissue with Ultrasound Vibrometry

Studies have found that shear moduli, having the dynamic range of several orders of magnitude for various biological tissues, are highly correlated with the pathological statues of human tissue such as livers. Shear moduli can be investigated by measuring the attenuation and velocity of the shear wave propagation in a tissue region. Many efforts have been made to measure shear wave propagations induced by different types of force, which include the motion force of human organs, external applied force, and ultrasound radiation force. In the past 15 years, ultrasound radiation force has been successfully used to induce tissue motion for imaging tissue elasticity. Vibroacoustography (VA) uses bifocal beams to remotely induce vibration in a tissue region and detect the vibration using a hydrophone. The vibration center is sequentially moved in the tissue region to form a two-dimensional image. Acoustic Radiation Force Imaging (ARFI) uses focused ultrasound to apply localized radiation force to small volumes of tissue for short durations and the resulting tissue displacements are mapped using ultrasonic correlation based methods. Supersonic shear image remotely vibrates tissue and sequentially moves vibration center along the beam axis to create intense shear plan wave that is imaged at a high frame rate (5000 frames per second). These image methods provide measurements of tissue elasticity, but not the viscosity. Because of the dispersive property of biological tissue, the induced tissue displacement and the shear wave propagation are frequency dependent. Tissue shear property can be modeled by several models including Kelvin-Voigt (Voigt) model, Maxwell model, and Zener model. The Voigt model effectively describes the creep behavior of tissue, The Maxwell model effectively describes the relaxation process, and the Zener model effectively describes both creep and relaxation but it requires one extra parameter. The Voigt model is often used by many researchers because of its simplicity and the effectiveness of modeling soft tissue. The Voigt model consists of a purely viscous damper and a purely elastic spring connected in parallel. For Voigt tissue, the tissue motion at a very low frequency largely depends on the elasticity, while the motion at a very high frequency largely depends on the viscosity. In general, the tissue motion depends on both elasticity and viscosity, and estimates of elasticity by ignoring viscosity are biased or erroneous. In 1951, Dr. Oestreicher published his work to solve the wave equation for the Voigt soft tissue with harmonic motions. With assumptions of isotropic tissue and plane wave, he derived equations that relate the shear wave attenuation and speed to the elasticity and viscosity of soft tissue. However, Oestreicher’s method was not realized for applications until the half century later. In the past ten years, Oestreicher’s method was utilized to quantitatively measure both tissue elasticity and viscosity. Ultrasound vibrometry has been developed to noninvasively and quantitatively measure tissue shear moduli. It induces shear waves using ultrasound radiation force and estimates the shear moduli using shear wave phase velocities at several frequencies by measuring the phase shifts of the propagating shear wave over a short distance using pulse echo ultrasound. Applications of the ultrasound vibrometry were conducted for viscoelasticities of liver, bovine and porcine striated muscles, blood vessels, and hearts. A recent in vivo liver study shows that the ultrasound vibrometry can be implemented on a clinical ultrasound scanner of using an array transducer. One potential application of ultrasound vibrometry is to characterize shear moduli of livers. The shear moduli of liver are highly correlated with liver pathology status. Recently, the shear viscoelasticity of liver tissue has been investigated by several research groups. Most of these studies applied ultrasound radiation force in liver tissue regions, measured the phase velocities of shear wave in a limited frequency range, and inversely solved the Voigt model with an assumption that liver local tissue is isotropic without considering boundary conditions. Because of the boundary conditions, shear wave propagations are impacted by the limited physical dimensions of tissue. Studies shows that considerations of boundary conditions should be taken for characterizing tissue that have limited physical dimensions such as heart, blood vessels, and liver, when ultrasound vibrometry is used