Effect of feed quality restriction followed by realimentation on growth, nutrient utilization, biochemical changes and haematological profiles of Indian major carp, rohu (Labeo rohita H.)

To investigate the effect of protein restriction with subsequent re-alimentation on nutrient utilization, hematological and biochemical changes of Indian major carp, Rohu (Labeo rohita H.), 150 acclimatized Rohu fingerlings (average 20.74 ± 0.13 g) divided into five experimental groups (30 fingerlings in each groups with three replications with 10 fingerlings in each) for experimental trial of 90 days using completely randomized design. Control group (T sub(CPR)) was fed with feed having 30% crude protein at 3% of body weight for 90 days trial period. Other experimental groups T sub(1PR) was alternatively 3 days fed with feed having 20% CP and 30% CP at 3% of body weight, T sub(2PR) was alternatively 7 days fed with feed having 20% CP and 30% CP at 3% of body weight, T sub(3PR) was alternatively 15 days fed with feed having 20% CP and 30% CP at 3% of body weight and T sub(4PR) was alternatively 25 days fed with feed having 20% CP and 30% CP at 3% of body weight during 90 days trial period with daily ration in two equal halves at morning and afternoon. It was noticed that retention of different nutrients was almost similar among all treatment groups indicated improvement of digestibility of nutrients might not be the mechanisms for recovery growth in carps. Increased percent feed intake of body weight (hyperphagia) (4.14 ± 0.30 or 4.94 ± 0.46 and 3.33 ± 0.29), improved specific growth rate (1.86 ± 0.09 or 2.26 ± 0.05 and 1.43 ± 0.01), absolute growth rate (1.57 ± 0.08 or 1.84 ± 0.18 and 1.36 ± 0.12), protein efficiency ratio (1.19 ± 0.11 or1.16 ± 0.12 and 1.05 ± 0.09) were the important mechanism showing better performance index (21.60 ± 1.09 or 23.80 ± 0.21 and 19.45 ± 0.37) through which the experimental groups which were protein restricted and re-alimented at 3 or 7 days alternatively during 90 days trial period could able to compensate the growth retardation and to catch up the final body weight of control (128.68 ± 11.53 g/f) but other experimental groups failed to compensate during 90 days trial period. Result of the present study indicated that deprived fish i.e., fish received alternate 3 or 7 days protein restriction and re-alimentation showed recovery growth had still lower values of Hb (10.21 ± 0.02, and 9.88 ± 0.04 g/dl), hematocrit value (30.62 ± 0.05 and 26.64 ± 0.11%), total erythrocytic count (3.40 ± 0.01 and 3.29 ± 0.01 X10super(6) mm³), plasma glucose (126.93 ± 0.20 and 126.67 ± 0.05 mg/dl), total plasma lipid (1.04 ± 0.01 and 1.02 ± 0.01 g/dl) and liver glycogen (290.10 ± 0.80 and 288.99 ± 0.95 mg/kg) in comparison to control (10.56 ± 0.08 g/dl, 31.68 ± 0.24%, 3.52 ± 0.03 X10super(6) mm³, 128.23 ± 0.25 mg/dl, 1.07 ± 0.01g/dl and 292.00 ± 0.23 mg/kg) at the end of 90 days trial but total plasma protein in deprived group was compensated with advancement of trial period. All hematological and biochemical parameters studied were proportionately lowered in the experimental group got higher degree of deprivation. These findings suggested that with the increase of trial length complete compensation of hematological and biochemical profiles of rohu might be achieved. The results indicated that the implementation of alternative 7 days low and high protein diet feeding during aquaculture of carps could make economize the operation through minimizing the feed input cost

Case Study: The Surgical Management of Angiokeratoma Resulting from Radiotherapy for Penile Cancer

Angiokeratoma is a rare, benign skin lesion and a recognised complication of radiation therapy. Here we describe a case of extensive angiokeratoma of the groin and external genitalia resulting from external beam radiation to that area in a patient with penile carcinoma. Furthermore, we outline the management of this problem by surgical reconstruction

Wearable Internet of Things - from Human Activity Tracking to Clinical Integration

Wearable devices for human activity tracking have been rapidly emerging. Most of them are capable of sending health statistics to smartphones, smartwatches or smart bands. However, they only provide the data for individual analysis and their data is not integrated into clinical practice. Leveraging on the Internet of Things (IoT), edge and cloud computing technologies, we propose an architecture which is capable of providing cloud based clinical services using human activity data. Such services could supplement the shortage of staff in primary healthcare centers thereby reducing the burden on healthcare service providers. The enormous amount of data created from such services could also be utilized for planning future therapies by studying recovery cycles of existing patients. We provide a prototype based on our architecture and discuss its salient features. We also provide use cases of our system in personalized and home based healthcare services. We propose an International Telecommunication Union based standardization (ITU-T) for our design and discuss future directions in wearable IoT

The Challenges of Wastewater Irrigation in Developing Countries

The volume of wastewater generated by domestic, industrial, and commercial sources has increased with population, urbanization, improved living conditions, and economic development. The productive use of wastewater has also increased, as millions of small-scale farmers in urban and peri-urban areas of developing countries depend on wastewater or wastewater-polluted water sources to irrigate high-value edible crops for urban markets, often as they have no alternative sources of irrigation water. Undesirable constituents in wastewater can harm human health and the environment. Hence, wastewater irrigation is an issue of concern to public agencies responsible for maintaining public health and environmental quality. For diverse reasons, many developing countries are still unable to implement comprehensive wastewater treatment programs. Therefore in the near term, risk management and interim solutions are needed to prevent adverse impacts from wastewater irrigation. A combination of source control and farm-level and post-harvest measures can be used to protect farm workers and consumers. The WHO guidelines revised in 2006 for wastewater use suggest measures beyond the traditional recommendations of producing only industrial or nonedible crops, as in many situations it is impossible to enforce a change in the current cash crop pattern, or provide alternative vegetable supply to urban markets. There are several opportunities for improving wastewater management via improved policies, institutional dialogues, and financial mechanisms, which would reduce the risks in agriculture. Effluent standards combined with incentives or enforcement can motivate improvements in water management by household and industrial sectors discharging wastewater from point sources. Segregation of chemical pollutants from urban waste-water facilitates treatment and reduces risk. Strengthening institutional capacity and establishing links between water delivery and sanitation sectors through interinstitutional coordination leads to more efficient management of wastewater and risk reduction

The Challenges of Wastewater Irrigation in Developing Countries

The volume of wastewater generated by domestic, industrial, and commercial sources has increased with population, urbanization, improved living conditions, and economic development. The productive use of wastewater has also increased, as millions of small-scale farmers in urban and peri-urban areas of developing countries depend on wastewater or wastewater-polluted water sources to irrigate high-value edible crops for urban markets, often as they have no alternative sources of irrigation water. Undesirable constituents in wastewater can harm human health and the environment. Hence, wastewater irrigation is an issue of concern to public agencies responsible for maintaining public health and environmental quality. For diverse reasons, many developing countries are still unable to implement comprehensive wastewater treatment programs. Therefore in the near term, risk management and interim solutions are needed to prevent adverse impacts from wastewater irrigation. A combination of source control and farm-level and post-harvest measures can be used to protect farm workers and consumers. The WHO guidelines revised in 2006 for wastewater use suggest measures beyond the traditional recommendations of producing only industrial or nonedible crops, as in many situations it is impossible to enforce a change in the current cash crop pattern, or provide alternative vegetable supply to urban markets. There are several opportunities for improving wastewater management via improved policies, institutional dialogues, and financial mechanisms, which would reduce the risks in agriculture. Effluent standards combined with incentives or enforcement can motivate improvements in water management by household and industrial sectors discharging wastewater from point sources. Segregation of chemical pollutants from urban waste-water facilitates treatment and reduces risk. Strengthening institutional capacity and establishing links between water delivery and sanitation sectors through interinstitutional coordination leads to more efficient management of wastewater and risk reduction

The Challenges of Wastewater Irrigation in Developing Countries

The volume of wastewater generated by domestic, industrial, and commercial sources has increased with population, urbanization, improved living conditions, and economic development. The productive use of wastewater has also increased, as millions of small-scale farmers in urban and peri-urban areas of developing countries depend on wastewater or wastewater-polluted water sources to irrigate high-value edible crops for urban markets, often as they have no alternative sources of irrigation water. Undesirable constituents in wastewater can harm human health and the environment. Hence, wastewater irrigation is an issue of concern to public agencies responsible for maintaining public health and environmental quality. For diverse reasons, many developing countries are still unable to implement comprehensive wastewater treatment programs. Therefore in the near term, risk management and interim solutions are needed to prevent adverse impacts from wastewater irrigation. A combination of source control and farm-level and post-harvest measures can be used to protect farm workers and consumers. The WHO guidelines revised in 2006 for wastewater use suggest measures beyond the traditional recommendations of producing only industrial or nonedible crops, as in many situations it is impossible to enforce a change in the current cash crop pattern, or provide alternative vegetable supply to urban markets. There are several opportunities for improving wastewater management via improved policies, institutional dialogues, and financial mechanisms, which would reduce the risks in agriculture. Effluent standards combined with incentives or enforcement can motivate improvements in water management by household and industrial sectors discharging wastewater from point sources. Segregation of chemical pollutants from urban waste-water facilitates treatment and reduces risk. Strengthening institutional capacity and establishing links between water delivery and sanitation sectors through interinstitutional coordination leads to more efficient management of wastewater and risk reduction

Anterior spinal separation surgery to allow for stereotactic body radiotherapy: a novel approach permitting radical oncological treatment of oligometastatic disease.

The treatment of spinal cancers has rapidly evolved in the past decade. Often the treatment for spinal metastases required highly morbid surgeries and with palliative outcomes. However, a paradigm shift in surgical oncology has allowed spinal metastases treatment to have curative results. In the state of oligometastatic disease (OMD), the accompaniment of Stereotactic Body Radiotherapy (SBRT) as a primary modality or adjuvant treatment to surgery has been shown to excellent survival outcomes, lower morbidities and better pain management. This case report illustrates a novel approach to the treatment of spinal OMD utilizing anterior spinal separation surgery with a custom carbon fibre vertebral body replacement cage followed by postoperative SBRT with excellent radio-oncological outcomes over 30-month follow-up

Pathway Editing Targets for Thiamine Biofortification in Rice Grains

Thiamine deficiency is common in populations consuming polished rice as a major source of carbohydrates. Thiamine is required to synthesize thiamine pyrophosphate (TPP), an essential cofactor of enzymes of central metabolism. Its biosynthesis pathway has been partially elucidated and the effect of overexpression of a few genes such as thi1 and thiC, on thiamine accumulation in rice has been reported. Based on current knowledge, this review focuses on the potential of gene editing in metabolic engineering of thiamine biosynthesis pathway to improve thiamine in rice grains. Candidate genes, suitable for modification of the structural part to evolve more efficient versions of enzymes in the pathway, are discussed. For example, adjacent cysteine residues may be introduced in the catalytic domain of thi4 to improve the turn over activity of thiamine thiazole synthase 2. Motif specific editing to modify promoter regulatory regions of genes is discussed to modulate gene expression. Editing cis acting regulatory elements in promoter region can shift the expression of transporters and thiamine binding proteins to endosperm. This can enhance dietary availability of thiamine from rice grains. Differential transcriptomics on rice varieties with contrasting grain thiamine and functional genomic studies will identify more strategic targets for editing in future. Developing functionally enhanced foods by biofortification is a sustainable approach to make diets wholesome