Emergence of central recirculation zone in a V-shaped premixed swirling flame

This paper presents an experimental study on the emergence of the central recirculation zone (CRZ) in a V-shaped premixed swirling flame, using simultaneous measurement of particle image velocimetry (PIV) and CH* chemiluminescence. The results show that either increasing the Reynolds number (Re) or decreasing the equivalence ratio ({\phi}) would facilitate the emergence of CRZ, and the inner shear layer (ISL) plays an essential role in governing the characteristics of CRZ. Further analysis demonstrates that the CRZ emergence can be promoted by higher ISL intensity but suppressed by enhanced viscous diffusion owing to higher flame temperature. As such, the CRZ formation can be interpreted as the outcome of a competition between the ISL intensity, i.e., circulation, and the vorticity consumption due to viscous diffusion. This competition physically corresponds to a special Reynolds number, Re_s, defined as the ratio between the ISL circulation ({\Gamma}) and the ISL effective viscosity ({\nu}_s), with a simplified heat loss model proposed for the temperature and viscosity estimations of the ISL. The outputting {\Gamma}-{\nu}_s plot yields a single boundary line separating the cases with and without CRZ, which points to a common critical Re_s of about 637, justifying the generality of the present criterion for lean-premixed V-shaped swirling flames of various operating conditions. Unlike most previous works which study the CRZ of a swirling flame from the point of vortex breakdown, the present work reveals the importance of enhanced viscous diffusion, caused by flame heating, in suppressing the CRZ emergence

Overexpression of the Wheat (Triticum aestivum L.) TaPEPKR2 Gene Enhances Heat and Dehydration Tolerance in Both Wheat and Arabidopsis

Wheat (Triticum aestivum L.) yield and quality are adversely affected by heat, drought, or the combination of these two stresses in many regions of the world. A phosphoenolpyruvate carboxylase kinase-related kinase gene, TaPEPKR2, was identified from our previous heat stress-responsive transcriptome analysis of heat susceptible and tolerant wheat cultivars. Based on the wheat cultivar Chinese Spring genome sequence, TaPEPKR2 was mapped to chromosome 5B. Expression analysis revealed that TaPEPKR2 was induced by heat and polyethylene glycol treatment. To analyze the function of TaPEPKR2 in wheat, we transformed it into the wheat cultivar Liaochun10, and observed that the transgenic lines exhibited enhanced heat and dehydration stress tolerance. To examine whether TaPEPKR2 exhibits the same function in dicotyledonous plants, we transformed it into Arabidopsis, and found that its overexpression functionally enhanced tolerance to heat and dehydration stresses. Our results imply that TaPEPKR2 plays an important role in both heat and dehydration stress tolerance, and could be utilized as a candidate gene in transgenic breeding

Functional Characterization of MicroRNA-27a-3p Expression in Human Polycystic Ovary Syndrome

The goal of this study was to characterize the function of microRNA-27a-3p (miR-27a-3p) in polycystic ovary syndrome (PCOS). miR-27a-3p expression was analyzed in excised granulosa cells (GCs) from 21 patients with PCOS and 12 normal patients undergoing in vitro fertilization cycle treatments and in 17 nontreated cuneiform ovarian resection PCOS samples and 13 control ovarian samples from patients without PCOS. We found that the expression of miR-27a-3p was significantly increased in both excised GCs and the ovaries of patients with PCOS compared with the controls. Insulin treatment of the human granulosa-like tumor cell line (KGN) resulted in decreased downregulated expression of miR-27a-3p, and this effect appeared to be mediated by signal transducer and activator of transcription STAT1 and STAT3. The overexpression of miR-27a-3p in KGN cells inhibited SMAD5, which in turn decreased cell proliferation and promoted cell apoptosis. After KGN cells were stimulated with insulin for 48 hours, there was increased expression of SMAD5 protein and decreased apoptosis. Additionally, knockdown/overexpression of SMAD5 in KGN cells reduced/increased cell number and promoted/inhibited cell apoptosis. Insulin-stimulated primary GCs isolated from patients with PCOS, in contrast to normal GCs or KGN cells, did not exhibit decreased miR-27a-3p expression. The differences in the expression levels in KGN cells and human PCOS GCs are likely explained by increased miR-27a-3p expression in the GCs caused by insulin resistance in PCOS. Taken together, our data provided evidence for a functional role of miR-27a-3p in the GCs' dysfunction that occurs in patients with PCOS

BRD4 Inhibitor Inhibits Colorectal Cancer Growth and Metastasis

Post-translational modifications have been identified to be of great importance in cancers and lysine acetylation, which can attract the multifunctional transcription factor BRD4, has been identified as a potential therapeutic target. In this paper, we identify that BRD4 has an important role in colorectal cancer; and that its inhibition substantially wipes out tumor cells. Treatment with inhibitor MS417 potently affects cancer cells, although such effects were not always outright necrosis or apoptosis. We report that BRD4 inhibition also limits distal metastasis by regulating several key proteins in the progression of epithelial-to-mesenchymal transition (EMT). This effect of BRD4 inhibitor is demonstrated via liver metastasis in animal model as well as migration and invasion experiments in vitro. Together, our results demonstrate a new application of BRD4 inhibitor that may be of clinical use by virtue of its ability to limit metastasis while also being tumorcidal

RFXB and its splice variant RFXBSV mediate the antagonism between IFNγ and TGFβ on COL1A2 transcription in vascular smooth muscle cells

Cytokines secreted by infiltrating immune cells during atherogenesis modulate vascular remodeling. One exemplary event is the antagonism between transformed growth factor (TGF-β) and interferon gamma (IFN-γ) on the transcriptional control of type I collagen gene (COL1A2). Previously we have reported that IFN-γ up-regulates regulatory factor for X-box B (RFXB) to repress collagen transcription while down-regulates the expression of RFXBSV, a splice variant of RFXB that blocks collagen repression in fibroblasts. Here we demonstrate that TGF-β abrogated COL1A2 repression by IFN-γ through altering the relative expression of RFXB and RFXBSV. Unlike RFXB, RFXBSV did not bind to the collagen promoter and competed with RFXB for the co-repressor histone deacetylase 2 (HDAC2), limiting HDAC2 recruitment to the collagen transcription start site as evidenced by chromatin immunoprecipitation assays. Over-expression of RFXB by lentiviral infection in HASMCs enhanced HDAC2 enlistment, promoted histone deacetylation surrounding the collagen site by IFN-γ, and blocked the TGF-β antagonism, a pattern reversed by RFXBSV infection. On the contrary, silencing of RFXB, but not both RFXB and RFXBSV, expression promoted the TGF-β antagonism. Thus, we have identified a novel mechanism whereby TGF-β antagonizes the IFN-γ repression of collagen transcription in HASMCs and as such provided new insights into antiatherogenic strategies

Monitoring of Wheat Height Based on Multi-GNSS Reflected Signals

Global Navigation Satellite System interferometric reflectometry (GNSS-IR), a new and inexpensive technique, has become available to the broader scientific community for detecting surface environmental information, such as soil moisture, snow depth and vegetation growth. However, there have been limited experiments focusing on the potential of crop height retrieval, especially the performance evaluation of BeiDou Navigation Satellite System (BDS) with other GNSS. Accuracy and reliability are challenging to achieve with traditional methods utilizing a single GNSS, and few measured verification data. In this study, an improved method that includes segmentation processing and multi-GNSS fusion is proposed based on GPS/GLONASS/Galileo/BDS multi-frequency data. Furthermore, experiments were carried out on a farmland in Fengqiu County, Henan Province, China. The results show that the height retrievals from four GNSS were in good agreement with the in situ observations during the whole growth cycle of the wheat after overwintering. Meanwhile, the retrievals based on the proposed method exhibited greater correspondence than the single frequency results, the correlation coefficient was increased and the root-mean-square error (RMSE) was reduced, respectively. Therefore, this study illustrates the feasibility of the proposed method to precisely estimate wheat height and its potential for use in the early warning of wheat lodging based on GNSS-IR

Monitoring of Wheat Height Based on Multi-GNSS Reflected Signals

Global Navigation Satellite System interferometric reflectometry (GNSS-IR), a new and inexpensive technique, has become available to the broader scientific community for detecting surface environmental information, such as soil moisture, snow depth and vegetation growth. However, there have been limited experiments focusing on the potential of crop height retrieval, especially the performance evaluation of BeiDou Navigation Satellite System (BDS) with other GNSS. Accuracy and reliability are challenging to achieve with traditional methods utilizing a single GNSS, and few measured verification data. In this study, an improved method that includes segmentation processing and multi-GNSS fusion is proposed based on GPS/GLONASS/Galileo/BDS multi-frequency data. Furthermore, experiments were carried out on a farmland in Fengqiu County, Henan Province, China. The results show that the height retrievals from four GNSS were in good agreement with the in situ observations during the whole growth cycle of the wheat after overwintering. Meanwhile, the retrievals based on the proposed method exhibited greater correspondence than the single frequency results, the correlation coefficient was increased and the root-mean-square error (RMSE) was reduced, respectively. Therefore, this study illustrates the feasibility of the proposed method to precisely estimate wheat height and its potential for use in the early warning of wheat lodging based on GNSS-IR

Research on Energy-Saving Scheduling of a Forging Stock Charging Furnace Based on an Improved SPEA2 Algorithm

In order to help the forging enterprise realize energy conservation and emission reduction, the scheduling problem of furnace heating was improved in this paper. Aiming at the charging problem of continuous heating furnace, a multi-objective furnace charging model with minimum capacity difference and waiting time was established in this paper. An improved strength Pareto evolutionary algorithm 2 (SPEA2) algorithm was designed to solve this problem. The original fitness assignment strategy, crossover operator and population selection mechanism of SPEA2 are replaced with DOPGA (Domination Power of an Individual Genetic Algorithm), adaptive cross operator, and elitist strategy. Finally, the effectiveness and feasibility of the improved SPEA2 was verified by actual arithmetic example. The comparison of results gained from three methods shows the superiority of the improved SPEA2 in solving this problem. Compared with strength Pareto evolutionary algorithm (SPEA) and SPEA2, the improved SPEA2 can get a better solution without increasing time complexity, the heating time is reduced by total 93 min, and can save 7533GJ energy. The research in this paper can help the forging enterprise improve furnace utilization, reduce heating time and unnecessary heating preservation time, as well as achieve sustainable energy savings and emissions reduction