UA-DETRAC: A New Benchmark and Protocol for Multi-Object Detection and Tracking

In recent years, numerous effective multi-object tracking (MOT) methods are developed because of the wide range of applications. Existing performance evaluations of MOT methods usually separate the object tracking step from the object detection step by using the same fixed object detection results for comparisons. In this work, we perform a comprehensive quantitative study on the effects of object detection accuracy to the overall MOT performance, using the new large-scale University at Albany DETection and tRACking (UA-DETRAC) benchmark dataset. The UA-DETRAC benchmark dataset consists of 100 challenging video sequences captured from real-world traffic scenes (over 140,000 frames with rich annotations, including occlusion, weather, vehicle category, truncation, and vehicle bounding boxes) for object detection, object tracking and MOT system. We evaluate complete MOT systems constructed from combinations of state-of-the-art object detection and object tracking methods. Our analysis shows the complex effects of object detection accuracy on MOT system performance. Based on these observations, we propose new evaluation tools and metrics for MOT systems that consider both object detection and object tracking for comprehensive analysis.Comment: 18 pages, 11 figures, accepted by CVI

Effect of Dihuang rougui decoction on ovariectomyinduced osteoporosis in rats

Purpose: To investigate the effect of Dihuang Rougui Decoction (DRD) on ovariectomy-induced osteoporosis in rats.Methods: Female Sprague-Dawley rats were randomly assigned to a  normal group (control) and five ovariectomy (OVX) subgroups: OVX with vehicle (OVX), OVX with positive control drug (alendronate sodium tablets, 1.6 mg/kg/week), and OVX + DRD (75, 150 or 300 mg/kg/day). After the rats were subjected to ovariectomy for 4 weeks, fosamax or DRD were administered daily (orally) for 16 weeks. The bone mineral density (BMD) of the L4 vertebrae and right femurs of the rats was evaluated. Serum hormones estradiol (E2), follicle-stimulating  hormone (FSH) and luteinizing hormone (LH) levels and serum alkaline phosphatase (ALP), osteocalcin (OC) and telopeptides of collagen type I (CTx) levels of the rats were determined. The bone tissue morphology of the rats was examined by microscopy.Results: The results show that DRD dose-dependently inhibited bone  mineral density (BMD) reduction of L4 vertebrae and femurs (both p < 0.05). DRD significantly increased serum E2, FSH and LH levels (p < 0.05) in the osteoporotic rats, and significantly lowered serum ALP, CTx and OC concentrations, compared to OVX group (p < 0.01). Compared with OVX model group, bone trabeculae in all three DRD groups and nilestriol groups were wider, and the space and connections markedly increased. Furthermore, the medullary cavity reduced in size.Conclusion: These findings indicate that DRD mitigates OVX-induced  osteoporosis in rats, and thus, the decoction has a potential for clinical  management of osteoporosis patients.Keywords: Dihuang Rougui decoction, Osteoporosis, Ovariectomy, Bone mineral density, Serum bone marker, Bone tissue morpholog

Screening and analysis of soda saline-alkali stress induced up- regulated genes in sugar sorghum

Soil salinization severely constrains the growth of crops, which ultimately leads to reduced yields. Because Sorghum dochna (common name sugar sorghum) has the advantageous properties of excellent salt stress resis- tance, high biomass, and tremendous flexibility for utilization as food, livestock feed, and industrial products, this species holds great potential to be further developed as a primary alternative crop. To elucidate the molecular mechanism that governs sugar sorghum’s adaptation to high salinity environments, we constructed a suppression subtractive hybridization (SSH) cDNA library from sugar sorghum transcripts that contains the soda saline-alkali induced up-regulated genes from the resistant variety M-81E. The SSH cDNA library was screened by using the colony hybridization method, and the ESTs obtained were sequenced and analyzed. A total of 200 EST clones were identified, representing 127 unigenes (6 contigs and 121 singlets). A Blast analysis showed that 48 ESTs (46.6%) have annotated functions in GenBank, 55 ESTs (53.4%) have unknown functions (or encode hypothetical proteins), and 24 ESTs (18.9%) have no blast hits. The majority of the hypothetical ESTs from the cDNA library displayed very high sequence similarity with their homologs found through GenBank. A clustering analysis of the ESTs with known functions indicated that a wide variety of genes were induced during the salt stress treatment. These genes were found to function in photosynthesis, material and energy metabolism (carbohydrates, lipids, amino acids, co-enzymes, ions, etc.), synthesis or maintenance of constituents of the cell wall and cell membrane, signal transduction, transcriptional regulation, and as water channels. This indicates that sugar sorghum tolerance to soda saline-alkali stress results from the coordinated functions of many genes

Strategies for Searching Video Content with Text Queries or Video Examples

The large number of user-generated videos uploaded on to the Internet everyday has led to many commercial video search engines, which mainly rely on text metadata for search. However, metadata is often lacking for user-generated videos, thus these videos are unsearchable by current search engines. Therefore, content-based video retrieval (CBVR) tackles this metadata-scarcity problem by directly analyzing the visual and audio streams of each video. CBVR encompasses multiple research topics, including low-level feature design, feature fusion, semantic detector training and video search/reranking. We present novel strategies in these topics to enhance CBVR in both accuracy and speed under different query inputs, including pure textual queries and query by video examples. Our proposed strategies have been incorporated into our submission for the TRECVID 2014 Multimedia Event Detection evaluation, where our system outperformed other submissions in both text queries and video example queries, thus demonstrating the effectiveness of our proposed approaches

The Pseudorabies Virus DNA Polymerase Accessory Subunit UL42 Directs Nuclear Transport of the Holoenzyme

Pseudorabies virus (PRV) DNA replication occurs in the nuclei of infected cells and requires the viral DNA polymerase. The PRV DNA polymerase comprises a catalytic subunit, UL30, and an accessory subunit, UL42, that confers processivity to the enzyme. Its nuclear localization is a prerequisite for its enzymatic function in the initiation of viral DNA replication. However, the mechanisms by which the PRV DNA polymerase holoenzyme enters the nucleus have not been determined. In this study, we characterized the nuclear import pathways of the PRV DNA polymerase catalytic and accessory subunits. Immunofluorescence analysis showed that UL42 localizes independently in the nucleus, whereas UL30 alone predominantly localizes in the cytoplasm. Intriguingly, the localization of UL30 was completely shifted to the nucleus when it was coexpressed with UL42, demonstrating that nuclear transport of UL30 occurs in an UL42-dependent manner. Deletion analysis and site-directed mutagenesis of the two proteins showed that UL42 contains a functional and transferable bipartite nuclear localization signal (NLS) at amino acids 354–370 and that K354, R355, and K367 are important for the NLS function, whereas UL30 has no NLS. Coimmunoprecipitation assays verified that UL42 interacts with importins α3 and α4 through its NLS. In vitro nuclear import assays demonstrated that nuclear accumulation of UL42 is a temperature- and energy-dependent process and requires both importins α and β, confirming that UL42 utilizes the importin α/β-mediated pathway for nuclear entry. In an UL42 NLS-null mutant, the UL42/UL30 heterodimer was completely confined to the cytoplasm when UL42 was coexpressed with UL30, indicating that UL30 utilizes the NLS function of UL42 for its translocation into the nucleus. Collectively, these findings suggest that UL42 contains an importin α/β-mediated bipartite NLS that transports the viral DNA polymerase holoenzyme into the nucleus in an in vitro expression system

A genetic study of the NOS3 gene for ischemic stroke in a Chinese population

We recruited 560 unrelated patients with ischemic stroke and 153 unrelated controls to undertake a genetic analysis for association between the NOS3 gene and ischemic stroke. All the subjects were Chinese of Han descent. Because the NOS3 gene spans about 21 kb of DNA and contains 26 exons, we selected a single nucleotide polymorphism (SNP) rs3918181, an A to G base change located in intron 14 of the gene, as a DNA marker. PCR-based restriction fragment length polymorphism analysis was applied to genotype rs3918181 (RsaI site). The chi-square (χ2) goodness-of-fit test showed that the genotypic distributions of the marker were not deviated from Hardy-Weinberg equilibrium in both the patient group (χ2 = 0.166, p = 0.684) and the control group (χ2 = 0.421, p = 0.517). The cocaphase analysis showed allelic association of rs3918181 with ischemic stroke in males (χ2 = 4.04, p = 0.044, OR = 1.43, 95% CI 1.01∼2.02) and frequency of allele A was significantly higher in male patients than male control subjects. The χ2 test revealed genotypic association between rs3918181 and ischemic stroke in males (χ2 = 4.26, df = 1, p = 0.039, OR = 1.61, 95% CI 1.02∼2.53) but not in females. The present work suggests that rs3918181 is associated with ischemic stroke in male patients. This finding gives further evidence in support of the eNOS association with ischemic stroke in the Chinese population

Conditional Adapters: Parameter-efficient Transfer Learning with Fast Inference

We propose Conditional Adapter (CoDA), a parameter-efficient transfer learning method that also improves inference efficiency. CoDA generalizes beyond standard adapter approaches to enable a new way of balancing speed and accuracy using conditional computation. Starting with an existing dense pretrained model, CoDA adds sparse activation together with a small number of new parameters and a light-weight training phase. Our experiments demonstrate that the CoDA approach provides an unexpectedly efficient way to transfer knowledge. Across a variety of language, vision, and speech tasks, CoDA achieves a 2x to 8x inference speed-up compared to the state-of-the-art Adapter approach with moderate to no accuracy loss and the same parameter efficiency

Screening and analysis of soda saline-alkali stress induced up- regulated genes in sugar sorghum

Soil salinization severely constrains the growth of crops, which ultimately leads to reduced yields. Because Sorghum dochna (common name sugar sorghum) has the advantageous properties of excellent salt stress resistance, high biomass, and tremendous flexibility for utilization as food, livestock feed, and industrial products, this species holds great potential to be further developed as a primary alternative crop. To elucidate the molecular mechanism that governs sugar sorghum’s adaptation to high salinity environments, we constructed a suppression subtractive hybridization (SSH) cDNA library from sugar sorghum transcripts that contains the soda saline-alkali induced up-regulated genes from the resistant variety M-81E. The SSH cDNA library was screened by using the colony hybridization method, and the ESTs obtained were sequenced and analyzed. A total of 200 EST clones were identified, representing 127 unigenes (6 contigs and 121 singlets). A Blast analysis showed that 48 ESTs (46.6%) have annotated functions in GenBank, 55 ESTs (53.4%) have unknown functions (or encode hypothetical proteins), and 24 ESTs (18.9%) have no blast hits. The majority of the hypothetical ESTs from the cDNA library displayed very high sequence similarity with their homologs found through GenBank. A clustering analysis of the ESTs with known functions indicated that a wide variety of genes were induced during the salt stress treatment. These genes were found to function in photosynthesis, material and energy metabolism (carbohydrates, lipids, amino acids, co-enzymes, ions, etc.), synthesis or maintenance of constituents of the cell wall and cell membrane, signal transduction, transcriptional regulation, and as water channels. This indicates that sugar sorghum tolerance to soda saline-alkali stress results from the coordinated functions of many genes