didymocarpoideae_tree

Phylogenetic tree for 56 Didymocarpoideae specie

primulina_data

Trait data of 100 species of the Primulina genu

R script

R script for PGLS regressions for the Didymocarpoideae subfamil

primulina_tree

Phylogenetic tree for 100 Primulina specie

R script

R script for measuring pylogenetic signal in the Didymocarpoideae subfamil

didymocarpoideae_data

Trait data of 56 species of the Didymocarpoideae subfamil

R script

R script for measuring pylogenetic signal in the Primulina genu

Flower trait dataset for Qst analysis

Flower trait dataset for Qst analysi

Der @Maler- und Lackierermeister : das deutsche Malermagzin

<div><p>Limestone Karst areas possess high levels of biodiversity and endemism. <i>Primulina</i> is a typical component of Karst endemic floras. The high species richness and wide distribution in various Karst microenvironments make the genus an idea model for studying speciation and local adaptation. In this study, we obtained 10 full-length sequences of the phytochrome <i>PHYE</i> from available transcriptome resources of <i>Primulina</i> and amplified partial sequences of <i>PHYE</i> from the genomic DNA of 74 <i>Primulina</i> species. Then, we used maximum-likelihood approaches to explore molecular evolution of <i>PHYE</i> in this Karst cave plant. The results showed that <i>PHYE</i> was dominated by purifying selection in both data sets, and two sites were identified as potentially under positive selection. Furthermore, the ω ratio varies greatly among different functional domains of <i>PHYE</i> and among different species lineages. These results suggest that potential positive selection in <i>PHYE</i> might have played an important role in the adaption of <i>Primulina</i> to heterogeneous light environments in Karst regions, and different species lineages might have been subjected to different selective pressures.</p></div

Sliding window analysis show variation of ω value along the <i>PHYE</i> gene from 74 partial sequences and 10 full-length sequences.

<p>The estimates were based on the Nei-Gojobori method. The window size was set at 30 bp and step size was set at 3 bp. Beneath the plot is a schematic of <i>PHYE</i>, which illustrates the distribution of the characteristic functional domains.</p