Effects of shear on eggs and larvae of striped bass, morone saxatilis, and white perch, M. americana

Shear stress, generated by water movement, can kill fish eggs and larvae by causing rotation or deformation. Through the use of an experimental apparatus, a series of shear (as dynes/cm2)-mortality equations for fixed time exposures were generated for striped bass and white perch eggs and larvae. Exposure of striped bass eggs to a shear level of 350 dynes/cm2 kills 36% of the eggs in 1 min; 69% in 2 min, and 88% in 4 min; exposure of larvae to 350 dynes/cm2 kills 9.3% in 1 min, 30.0% in 2 min, and 68.1% in 4 min. A shear level of 350 dynes/cm2 kills 38% of the white perch eggs in 1 min, 41% in 2 min, 89% in 5 min, 96% in 10 min, and 98% in 20 min. A shear level of 350 dynes/cm2 applied to white perch larvae destroys 38% of the larvae in 1 min, 52% in 2 min, and 75% in 4 min. Results are experimentally used in conjunction with the determination of shear levels in the Chesapeake and Delaware Canal and ship movement for the estimation of fish egg and larval mortalities in the field

Rat white adipocytes activate p85/p110 PI3K and induce PM GLUT4 in response to adrenoceptor agonists or aluminum fluoride

Adipocyte responses to adrenergic and ß-adrenoceptor(-AR) (adrenoceptor) regulation are not sufficiently understood, and information helpful for elucidating the adrenoceptor-responsive machinery is insufficient. Here we show by using immunoprecipitated kinase analysis with a phosphatidylinositol 3-kinase (PI3K) p85 antibody that PI3K activation was induced by treatment with 10 or 100 µM norepinephrine (NE) for 15 min or with 10 mM aluminum fluoride (AF, a guanosine triphosphate (GTP)-binding (G) protein activator) for 20 min in white adipocytes (rat epididymal adipocytes) and that treatment with pertussis toxin (PTX, a G-protein inactivator) inhibited PI3K activation induced by the 20-min treatment with AF in the cells. In addition, western blot analysis revealed that glucose transporter 4 (GLUT4) level in the adipocyte plasma membrane (PM) fraction was increased by treatment with 10 µM NE, 100 µM dobutamine (DOB, a ß1-AR agonist), or 0.1 µM CL316243 (CL, a ß3-AR agonist) for 30 min or with 10 mM AF for 20 min. NE or AF treatment triggered 2-deoxyglucose (2-DG) uptake into adipocytes under the above conditions. Our results advance the understanding of responses to adrenoceptor regulation in white adipocytes and provide possible clues for clarifying the machinery involved in adrenergic and ß-AR responses in the cells

Y-type Flux-Tube Formation in Baryons

For more than 300 different patterns of the 3Q systems, the ground-state 3Q potential $V_{\rm 3Q}^{\rm g.s.}$ is investigated using SU(3) lattice QCD with $12^3\times 24$ at $\beta=5.7$ and $16^3\times 32$ at $\beta=5.8, 6.0$ at the quenched level. As a result of the detailed analyses, we find that the ground-state potential $V_{\rm 3Q}^{\rm g.s.}$ is well described with so-called Y-ansatz as $V_{\rm 3Q}=-A_{\rm 3Q}\sum_{i<j}\frac1{|{\bf r}_i-{\bf r}_j|} +\sigma_{\rm 3Q} L_{\rm min}+C_{\rm 3Q}$, with the accuracy better than 1%. Here, $L_{\rm min}$ denotes the minimal value of total flux-tube length. We also studythe excited-state potential $V_{\rm 3Q}^{\rm e.s.}$ using lattice QCD with $16^3\times 32$ at $\beta=5.8, 6.0$ for more than 100 patterns of the 3Q systems. The energy gap between $V_{\rm 3Q}^{\rm g.s.}$ and $V_{\rm 3Q}^{\rm e.s.}$, which physically means the gluonic excitation energy, is found to be about 1 GeV in the typical hadronic scale. Finally, we suggest a possible scenario which connects the success of the quark model to QCD.Comment: Talk given at Color Confinement and Hadrons in Quantum Chromodynamics (Confinement 2003), Saitama, Japan, 21-24 July 2003; 5 pages, 4 figure

Influencia de factores fósicos y químicos sobre la sobrevivencia de Pichia aislada de jarabe de glucosa

Yeasts belonging to the genus Pichia were isolated fr om glucose syrup samples. Yeasts were identified as P. anomala ( it is now Wickerhamomyces anomala) and P. guilliermondii. These strains did not metabolize the nutritive preservatives, potassium sorbate or sodium benzoate when these were used as the single carbon source. P. anomala grew in culture media containing up to 1200 mg/L of both preservatives. Critical temperature and time of exposure for its inactivation were 60 °C and 3 min, respectively. P. guilliermondii grew in media containing up to 1400 mg/L of both preservatives. Critical temperature and time for inactivation of this Pichia were 80 °C and 2 min. This strain was able to grow in a wide range of temperatures (5 to 30 °C), pH (2.5 to 5.5) and glucose concentrations (200 to 800 g/L). At 5 °C and 800 g/L glucose (osmotic pressure, 0.110 atm), P.guilliermndii gr ew poorly , with no cell death because of its ability to sporulate. We determined that P. guilliermondii is a potentially contaminating yeast able to develop in a variety of foods, especially those with low pH or with high sugar concentrations (glucose above 400g/L) such as refreshments, juices, syrups and confected fruits and it is resistant to both food preservatives and low temperatures (5°C).De muestras de jarabe de glucosa fueron aisladas levaduras que pertenecen al género Pichia. Estas fueron identificadas como P.anomala (actualmente Wickerha- momyces anomala) y P. guilliermondii, ambas cepas no metabolizan los conservantes alimenticios, sorbato de potasio o benzoato de sodio, al ser usados como única fuente de carbono. P. anomala desarrolló en medios de cultivo que contenían hasta 1200 mg/L de ambos conservantes. La temperatura y el tiempo crítico de exposición para su inactivación fueron 60 °C y 3 min, respectiva- mente. P.guilliermondii desarrolló en medios que contienen hasta 1400 mg/l de ambos conservantes. La to y el tiempo crítico de inactivación fueron 80 °C y 2 min. Esta cepa fue capaz de desarrollar en un amplio rango de temperaturas (5 a 30 °C), pH (2,5 a 5,5) y concentraciones de glucosa (200 a 800 g/L). A 5 °C y glucosa 800 g/L (presión osmótica, 0,110 atm), P. guilliermondii desarrolló pobremente sin que se produzca la muerte celular debido a su capacidad de esporular. Se deter- minó que P. guilliermondii, es una levadura potencialmente contaminante que puede desarrollarse en una variedad de alimentos, especialmente aquellos con bajo pH o altas concentraciones de azúcar (glucosa, > 400 g/L) como en gaseosas, jugos, jarabes y frutas confitadas, y es resistente a ambos conservantes y bajas to (5°C)Fil: Rubio, María Cristina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Biotecnología; ArgentinaFil: Navarro, Antonio Roberto. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentin

Average group effect of strongly correlated predictor variables is estimable

It is well known that individual parameters of strongly correlated predictor variables in a linear model cannot be accurately estimated by the least squares regression due to multicollinearity generated by such variables. Surprisingly, an average of these parameters can be extremely accurately estimated. We find this average and briefly discuss its applications in the least squares regression.Comment: 1