Supplementary material for the article: Šoštarić, T. D.; Petrović, M. S.; Pastor, F. T.; Lončarević, D. R.; Petrović, J. T.; Milojković, J. V.; Stojanović, M. D. Study of Heavy Metals Biosorption on Native and Alkali-Treated Apricot Shells and Its Application in Wastewater Treatment. Journal of Molecular Liquids 2018, 259, 340–349. https://doi.org/10.1016/j.molliq.2018.03.055

Supplementary material for: [https://doi.org/10.1016/j.molliq.2018.03.055]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2152]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/2970

Effects of different mechanical treatments on structural changes of lignocellulosic waste biomass and subsequent Cu(II) removal kinetics

In this paper, the character of structural changes induced by different mechanical treatments to Prunus persica stones (PSs), and its subsequent effect on biosorption kinetics of Cu(II) were investigated. PSs were processed in vibratory disk mill (PS-V) and ultra-centrifugal mill (PS-C) and characterized by XRD, BET, SEM and FTIR spectroscopy. It was shown that PS-V was smaller and more reactive with less crystallinity index and hydrogen bond intensity compared to PS-C. In opposite, surface area of the PS-C was bigger than that of the PS-V. The total pore volume was about threefold, while the volume of micro pores was 9.29 times higher in PS-Cs than in PS-Vs. The kinetics of Cu(II) biosorption by both PSs was tested through various kinetic models: pseudo-first and pseudo-second order rate equations, Elovich equation, Boyd model, Weber–Morris and Urano–Tachikawa intraparticle diffusion model. For both sample types, Cu(II) biosorption occurred through combination of intraparticle and film diffusion mechanism, while kinetic results were best described by the pseudo-second order kinetic model. At the same time, the results indicated that together with kinetic rate the biosorption capacity of PS-C (21.20 mg g−1) was higher than that of PS-V (16.30 mg g−1). Mechanical activation like crushing and grinding will change material particle size, specific surface area and porosity, as well as its crystallinity. However, this paper elucidates that such physical structural changes will impact on heavy metal ions removal efficiency. This investigation suggests that the type of size reduction in lignocellulosic biosorbent preparation plays a very important role in overall biosorption performance, so it should be carefully considered every time when the mechanical treatment of material is necessary to be applied

Supplementary material for the article: Milojković, J. V.; Popović-Djordjević, J. B.; Pezo, L. L.; Brčeski, I. D.; Kostić, A. Ž.; Milošević, V. D.; Stojanović, M. D. Applying Multi-Criteria Analysis for Preliminary Assessment of the Properties of Alginate Immobilized Myriophyllum Spicatum in Lake Water Samples. Water research 2018, 141, 163–171. https://doi.org/10.1016/j.watres.2018.05.014

Supplementary material for: [https://doi.org/10.1016/j.watres.2018.05.014]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2186]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3029

In vitro uklanjanje mikotoksina korišćenjem različitih neorganskih adsorbenata i organskih otpadnih materijala iz Srbije

Aflatoxin B1 (AFB1), ochratoxin A (OTA), zearalenone (ZON), deoxynivalenol (DON) and T-2 toxin are the most extensively studied toxic fungal metabolites. Once mycotoxins enter the food/feed production chain keeping their toxic characteristics, it is very difficult to remove or eliminate them. One of promising methods to reduce mycotoxins in contaminated food/feedstuffs is the use of mycotoxin binders. This paper presents the results of in vitro investigations of mineral mycotoxin binders (bentonite - BEN, diatomite - DIA and zeolite - ZEO), and organic mycotoxin binders - agricultural waste materials (Myriophillium spicatum, peach and sour cherry pits). Chemical compositions of the adsorbents have showed that they do not consist of elements toxic to the animals. Inorganic adsorbents (BEN, DIA and ZEO) tested in vitro were better binders of AFB1 (94.97% - 96.90%), while the biosorbents were more efficient in adsorption of OTA (19.98% - 66.66%), ZON (33.33% - 75.00%) and T-2 toxin (16.67% - 50.00%). Inorganic adsorbents and organic waste materials expressed similar binding capacity for DON in vitro, with the exception of M. spicatum that did not at all adsorb this type B trichothecene. Our results indicate that feed contamination with different types of mycotoxins might be diminished by a product that combines different inorganic and organic adsorbents with diverse mycotoxin binding properties.Aflatoksin B1 (AFB1), ohratoksin A (OTA), zearalenon (ZON), dezoksinivalenol (DON) i T-2 toksin su najviše izučavani toksični metaboliti gljiva. Kada mikotoksini uđu u proizvodni lanac za hranu/hranu za životinje, zadržavajući svoje toksične karakteristike, teško ih je ukloniti ili eliminisati. Jedna od obećavajućih metoda za smanjenje nivoa mikotoksina u kontaminiranoj hrani/hrani za životinje je korišćenje mikotoksinskih veziva. Ovaj rad predstavlja rezultate in vitro istraživanja mineralnih mikotoksinskih veziva (bentonit - BEN, diatomit - DIA i zeolit - ZEO) i organskih veziva mikotoksina - poljoprivrednog otpadnog materijala (Myriophillium spicatum, koštice breskve i višnje). Hemijski sastavi adsorbenata pokazali su da ne sadrže elemente toksične za životinje. Neorganski adsorbenti (BEN, DIA i ZEO) testirani in vitro bolje su vezivali AFB1 (94,97% - 96,90%), dok su biosorbenti bili efikasniji u adsorpciji OTA (19,98% - 66,66%), ZON-a (33,33% - 75,00% ) i T-2 toksina (16,67% - 50,00%). Neorganski adsorbenti i organski otpadni materijali su pokazali sličan kapacitet in vitro vezivanja DON-a, sa izuzetkom M. spicatum koji uopšte nije adsorbovao ovaj trihotecen tipa B. Naši rezultati koji su prikazani ovde pokazuju da zagađivanje hrane i hrane za životinje različitim vrstama mikotoksina može biti smanjeno dodavanjem preparata dobijenog kombinacijom različitih neorganskih i organskih adsorbenata koji poseduju različite karakteristike vezivanja mikotoksina

Natural deep eutectic solvent as extraction media for the main phenolic compounds from Rubi idaei leaves

Upper-respiratory-tract infections (URTI) are contagious diseases caused by a variety of bacteria and viruses. Based on European Union reports for Serbia, usage of antibiotics in pediatrics is extremely high compared to other European countries. Thus, there is growing demand for herbal-based products for the prevention of URTI as an alternative for antibiotics. Due to therapeutic properties such as antimicrobial, cytotoxic, antioxidative, and anti-inflammatory Rubi idaei leaves were used in Serbian folk medicine against URTI. Additionally, commercially available herbal products most often comprise ethanolic extracts. However, high-alcohol content makes such products unsuitable for patients’ intolerant to alcohol, especially children or other sensitive group of patients. Due to mentioned drawbacks, proposed study was focus on the development of novel natural, edible, and safe alternative using NADES (Natural Deep Eutectic Solvent), as a new-generation solvents that resemble the plants’ intracellular environment. Due to the unique, tuneable solvation properties, NADES are getting more attention as solvents in natural product research. This study aimed to present the green approach by using ultrasound assisted extraction and twenty various NADES systems as well as ethanol, methanol, and water as control solvents. The obtained extracts were evaluated in terms of their chemical composition by high-performance thin-layer chromatography (HPTLC), as well as in individual compounds by high-performance liquid chromatography (HPLC) coupled with mass spectrometry. Chromatographic profiles revealed differences in chemical composition depending on the applied extraction system. Ten phenolic compounds such as chlorogenic acid, caffeic acid, rutin, isoquercetin, astragalin, isorhamnetin 3-O-glucoside, apigetrin, quercetin, kaempferol were quantified in Rubi Idaei leaves. Astragalin, isoquercetin, and rutin were major Consituents, while other compounds were found in minor amount. Kaempferol was found in high amount in almost all extracts, except in systems cholin chloride : glycerol with higher amount of water and cholin chloride : urea. Chemometric evaluation was applied to compare the extraction profile of NADES systems and their quantitative composition. Extraction systems discrimination revealed by principal component analysis confirming specific chemical profiles depending on the solvents used (Figure 1)

Biosorpcija kao nova biotehnologija u funkciji očuvanja i uravnoteženog korišćenja biodiverziteta

Rapid techno-economic development, without adequate protection of the environment, caused a number of emergence problems: pollution, waste, loss of biodiversity, introduction of invasive species, release of o genetically modified organisms etc. Protection, conservation of natural resources and biodiversity requires an integrated approach to sustainable management. One approach is to stimulate the development of new biotechnology with an aim of saving and balanced usage of biodiversity. An increased industrial activity has intensified more environmental problems such as deterioration of several ecosystems due to the accumulation of pollutants. A vast array of biological materials, especially bacteria, algae, yeasts and fungi have received increasing attention for heavy metal removal due to their good performance, low cost and large available quantities. Biosorption has been defined as the property of certain biomolecules (or types of biomass) to bind and concentrate selected ions or other molecules from aqueous solutions. Biosorbents, unlike mono functional ion exchange resins, contains variety of functional sites. Advantages of these materials are their efficiency and low cost and application of biosorbents is good solution for removing metals, especially heavy metals from aqueous solutions. Different types of biosorbents that were considered for waste are getting a new application with extending product life cycle. Biosorption needs a multidisciplinary approach from the point of chemical, microbiological and process engineering, and allows a wider range of remediation techniques in relation to the ion exchange. This paper presents an overview of trends in biosorption and application of biosorbents for removing the pollutants (heavy metals and radionuclides) from contaminated effluents and water. .Ubrzan tehno-ekonomski razvoj, bez adekvatne zaštite životne sredine, je prouzrokovao pojavu velikog broja problema: zagađenje, otpad, gubitak biodiverziteta, uvođenje invazivnih vrsta, oslobađanje genetski modifikovanih organizama, itd. Zaštita i očuvanje prirodnih dobara i biodiverziteta zahteva integralni pristup u održivom upravljanju. Jedan od pristupa je i stimulisanje razvoja novih biotehnologija u funkciji očuvanja i uravnoteženog korišćenja biodiverziteta. Povećana industrijska proizvodnja je dovela do ozbiljnih problema u životnoj sredini uzrokujući starenje nekoliko ekosistema zbog akumulacije polutanata. Veliko interesovanje u svetu postoji za biološkim materijalom, bakterijama, algama, kvascima i gljivama, kao biosorbentima za uklanjanje teških metala, zbog njihove niske cene, dobrih performansi i dostupnosti u većim količinama. Biosorpcija se može definisati kao sposobnost određenih biomolekula (ili tipova biomase) da vežu ili koncentrišu određene jone ili druge molekule iz vodenih rastvora. Biosorbenti za razliku od monofunkcionalnih jonoizmenjivača, sadrže različite funkcionalne grupe. Ovi materijali, kao jeftini ali efikasni adsorbenti, predstavljaju dobro rešenje za uklanjanje metala, posebno teških metala iz vodenih rastvora. Različite vrste biosorbenata koje su smatrane za otpad dobijaju novu primenu i produžava im se životni ciklus. Pristup biosorpciji je multidisciplinaran sa tačke hemijskog, mikrobiološkog i procesnog inženjerstva i omogućava širi spektar remedijacionih tehnika u odnosu na jonsku izmenu. Ovaj rad predstavlja pregled savremenih trendova u biosorpciji i prikaz korišćenih biosorbenta za uklanjanje polutanata (teški metali i radionuklidi) iz kontaminiranih efluenata i voda.

Lignocellulosic Waste Material - from Landfill to Sorbent and Fuel

The most important source of renewable energy in Serbia represents biomass (60.3 %) with total potential of 3.405 million toe. Almost half of this amount (1.67 million toe) represents agricultural and industrial waste, with further negligible revalorization. Various investigations have demonstrated that agricultural byproducts have promising capacities to remove a variety of pollutants, which might increase the sustainability of their life cycle. This paper investigates the possible use of lignocellulosic waste material (LCW), originating from food industry as biosorbent for heavy metals, and later as a solid fuel. For this purpose we have used peach stone particles (PS) obtained by mechanical activation of this LCW as Cu(II) sorbent. The physical and chemical characteristics of PS reveal its complex structure which was confirmed by SEM, and FTIR analysis. PS behavior on pyrolysis process was studied by dynamic thermo gravimetric and derivate thermo gravimetric analysis. Results show that this lignocellulosic waste can be applied as sorbent and as a fuel. This approve that this agricultural waste can be a resource more than a waste and that it does not need to be disposed of in the costly and inefficient way, which is especially important in developing economies, like Serbian

Nova tehnologija za tretman podzemnih i industrijskih voda kontaminiranih naftnim ugljovodnicima

Ovo tehničko rešenje se odnosi na rešavanje problema prečišćavanja industrijskih i podzemnih voda kontaminiranih naftnim zagađivačima. Ono daje prikaz kombinovane tehnike prečišćavanja voda kontaminiranih naftnim ugljovodonicima primenom mehanički tretiranog sorbenta na bazi koštica breskve (otpad iz industrije za preradu voća) koji pored uloge sorbenta imai ulogu nosača mikroorganizama u biološkom procesu remedijacije.Техничко решење из категорије М8

Study of heavy metals biosorption on native and alkali-treated apricot shells and its application in wastewater treatment

Locally available apricot (Prunus armeniaca L.) shells classified as a waste product from fruit processing, were alkali activated in order to develop an efficient heavy metal ions sorbent for water purification. To examine the changes occurred after alkali treatment, raw (SH) and modified apricot shells (SHM) were thoroughly characterized in terms of their chemical composition and surface properties. Chemical analysis revealed that alkaline treatment causes the disintegration of hemicellulose (its content decreased from 19.2 to 3.5%), which was in accordance with FTIR results. SEM micrographs and the mercury intrusion porosimetry revealed a larger surface area and porosity of SHM. Bohem's acid-base titration method indicated that the most of the SHM surface carboxylic groups were in sodium salt form and together with the pH of points of zero charge showed increase of surface alkalinity after modification. Treatment with NaOH enhanced the adsorption capacity by 154, 61 and 90% for Cu2+, Zn2+ and Pb2+, respectively. The amount of cations released from SHM was almost equal to the amount of adsorbed metal ions, suggesting ion exchange mechanism. The pseudo-second order kinetic indicated that the heavy metals cations were bound predominantly by complexation. In order to establish the effectiveness of the biosorbent in real wastewater sample, SHM was employed for cleaning-up of drain water emanating from atomic adsorption spectrophotometer. The SHM showed high removal efficiency towards multiple metal ions. The amounts of Fe, Pb, Cu and Cr ions were reduced by 97, 87, 81 and 80%, respectively, while Ni and Zn amounts were reduced for 33 and 14%. Used biosorbent SHM can be successfully regenerated with HCl (desorption gt 95%) and after regeneration biosorbent can be reused or it can be safely disposed. (C) 2018 Elsevier B.V. All rights reserved.Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/2969]Peer-reviewed manuscript: [http://cherry.chem.bg.ac.rs/handle/123456789/2970

Efficient phytoremediation of uranium mine tailings by tobacco

This investigation shows that tobacco plant roots and leaves accumulate 60 times more uranium than previously reported. Phytoremediation is a convenient technique to clean up polluted soils using herbaceous plants and trees. Increasing research aims to identify novel plant species that accumulate toxic metals. Tobacco plant (Nicotiana tabacum L.) is a promising cultivar for phytoremediation because tobacco is fast growing and easily propagated. Here, we study phytoremediation of uranium by two tobacco varieties Virginia and Burley, bred in natural conditions. Plants were grown on uranium mine tailings with an average uranium content of 15. 3 mg kg -1. Each shoot sample was cross-sectioned into five uniform groups of leaves and stem segments. Results show a substantial variance in uranium uptake according to the section elderliness and origin of the plant parts. The highest concentrations of uranium values recorded in leaves of Burleys and Virginias nearest root shoot sections were 4. 18 and 3. 50 mg kg -1, respectively. These values are 60 times higher rates than those previously published for leaves of cultivars grown under similar conditions. Taking into account the level of soil contamination, the content of accumulated uranium demonstrates uranium hyperaccumulatory properties of tobacco plant and its potential utilization in phytoremediation of uranium-contaminated mediums