Visible light-induced destabilization of endocytosed liposomes

AbstractThe potential biomedical utility of the photoinduced destabilization of liposomes depends in part on the use of green to near infrared light with its inherent therapeutic advantages. The polymerization of bilayers can be sensitized to green light by associating selected amphiphilic cyanine dyes, i.e. the cationic 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine (DiI), or the corresponding anionic disulfonated DiI (DiI-DS), with the lipid bilayer. The DiI sensitization of the polymerization of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine/1,2-bis[10-(2′,4′-hexadienoyloxy)-decanoyl]-sn-glycero-3-phosphocholine liposomes caused liposome destabilization with release of encapsulated aqueous markers. In separate experiments, similar photosensitive liposomes were endocytosed by cultured HeLa cells. Exposure of the cells and liposomes to 550 nm light caused a net movement of the liposome-encapsulated 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) from low pH compartment(s) to higher pH compartment(s). This suggests that photolysis of DiI-labelled liposomes results in delivery of the contents of the endocytosed liposomes to the cytoplasm. The release of HPTS into the cytoplasm appears to require the photoactivated fusion of the labelled liposomes with the endosomal membrane. These studies aid in the design of visible light sensitive liposomes for the delivery of liposome-encapsulated reagents to the cytoplasm

Heat inactivation of Listeria innocua in broth and food products under non-isothermal conditions

The objective of this work was to study the effect of three linear temperature profiles (heating rates of 1.5, 1.8 and 2.6 °C/min, from 20 to 65 °C) on Listeria innocua inactivation in liquid medium. The inactivation was also analyzed in artificially contaminated parsley (heating rate of 1.8 °C/min) and throughout a frying process, using a pre-cooked frozen food as case study. Inactivation showed a sigmoidal behaviour and all data was fitted with a Gompertz-inspired model. Results demonstrated that, in liquid media, Listeria inactivation is influenced by the temperature profile used. As heating rate increases, the shoulder decreases and the tail effect disappears. If Listeria was in parsley, its heat resistance increased (for identical experimental conditions in broth). Besides model adequacy was proven in all studied situations, the heating rate affected parameters’ precision.info:eu-repo/semantics/acceptedVersio

Thermal torque effects on the migration of growing low-mass planets

As planets grow the exchange of angular momentum with the gaseous component ofthe protoplanetary disc produces a net torque resulting in a variation of the semi-major axis of the planet. For low-mass planets not able to open a gap in the gaseousdisc this regime is known as type I migration. Pioneer works studied this mechanismin isothermal discs finding fast inward type I migration rates that were unable toreproduce the observed properties of extrasolar planets. In the last years, several im-provements have been made in order to extend the study of type I migration rates tonon-isothermal discs. Moreover, it was recently shown that if the planet?s luminositydue to solid accretion is taken into account, inward migration could be slowed downand even reversed. In this work, we study the planet formation process incorporating,and comparing, updated type I migration rates for non-isothermal discs and the role ofplanet?s luminosity over such rates. We find that the latter can have important effectson planetary evolution, producing a significant outward migration for the growingplanets.Fil: Guilera, Octavio Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Cuello, N.. Pontificia Universidad Católica de Chile; ChileFil: Montesinos, M.. Universidad de Valparaíso; ChileFil: Miller Bertolami, Marcelo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Ronco, María Paula. Pontificia Universidad Católica de Chile; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Cuadra, J.. Pontificia Universidad Católica de Chile; ChileFil: Masset, F. S.. Universidad Autonoma de Mexico; Méxic

ASCI Red for dummies : a recipe book for easy use of the ASCI Red platform.

It has been recognized that documentation for new customers of ASCI Red, aka janus or the Intel Teraflops at Sandia National Laboratories, has been sadly lacking. This document has been prepared by a team of subject matter experts to fill that void and to provide a starting point for providing a similar document for ASCI Red Storm in the future. This document is intended for SNL users who need to jumpstart their use of Janus and Janus-s

Family presence and participation during medical visits of heart failure patients: An analysis of survey and audiotaped communication data

To describe the frequency, roles, and utility of family companion involvement in the care of patients with Heart Failure (HF) care and to examine the association between audiotaped patient, companion, and provider communication behaviors