11 research outputs found
Detection of Poxvirus Using Transmission Electron Microscopy Techniques During Outbreak in Bay-Winged Cowbird (Gnorimopsar Chopi)
Occurrence of parasitism by Dioctophyma renale in ring-tailed coatis (Nasua nasua) of the Tiete Ecological Park, São Paulo, Brazil
Research of Torque teno virus (TTV) in Serum and Total Blood of Brazilian Non-Human Primates and in Chicken Plasma (Gallus gallus domesticus) by the PCR N22 Region
Torque Teno Virus (TTV) is Prevalent in Brazilian Non-human Primates and Chickens (Gallus gallus domesticus)
Investigación de Torque teno virus (TTV) en Suero y Sangre de Primates No Humanos Brasileños y en Plasma de Pollo (Gallus gallus domesticus) por PCR en la Region N22
Torque teno virus (TTV) is a recently discovered DNA virus that was originally isolated from a Japanese patient ( initials, TT) with post-transfusion hepatitis of unknown aetiology. TTV is an circular DNA virus classified recently together with related Torque teno minivirus, into a new genus called Anellovirus. Infection TTV has been detected in a range of non-human primates as well as domestic animals. The purpose of this study was to search TTV in the serum and total blood of Brazilian monkeys and in plasma of domestic chickens by seminested PCR of coding region (N22), followed by a genomic sequence and phylogenetic analysis. No serum sample was amplified. TTV DNA was detected in total blood from 3 (4%) out of 75 brown-capuchin ( Cebus apella) and from 1 (25%) out of 4 golden-headed lion-tamarin ( Leontopithecus chrysomelas). Phylogenetic analysis revealed that one sample showed similarity with one sequence of the cotton top tamarin ( Saguinus oedipus) (So-TTV2) and with one of the douroucoulis (Aotes trivirgatus) (At-TTV3). Two samples showed similarity with a human Torque Teno Mini Virus (TLMV). The other sample clustered with one sequence of the chimpanzee (Pt-TTV6) and with the human TTV strain TA278. The plasma chicken samples tested were all negative. The amino acid sequences reported in this study are the first obtained in Brazil from total blood of non-human primates naturally infected by TTV.Biol Inst Sao Paulo, Electron Microscopy Lab, BR-04014002 Sao Paulo, BrazilUniv Fed Sao Paulo, Lab Retrovirol, Sao Paulo, BrazilFundaCao Problood Homectr Sao Paulo, Sao Paulo, BrazilUniv Sao Paulo, Inst Biomed Sci, BR-05508 Sao Paulo, BrazilInst Adolfo Lutz Registro, Sao Paulo, BrazilEcol Pk Tiete, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Surg Tech, Sao Paulo, BrazilUniv Fed Sao Paulo, Lab Retrovirol, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Surg Tech, Sao Paulo, BrazilWeb of Scienc
Ocorrência de Dioctophyma renale em Galictis cuja
A dioctofimose é uma parasitose causada pelo Dioctophyma renale (Goeze, 1782) de ocorrência mundial e acomete animais domésticos e silvestres. Em março de 2010, um exemplar adulto (macho) de Galictis cuja (Molina, 1782), encontrado morto por atropelamento no município de Guaíra, Paraná foi encaminhado ao laboratório de Patologia Veterinária de Universidade Federal do Paraná (UFPR), Campus Palotina, para a realização da necropsia. O cadáver apresentava bom estado nutricional e autólise moderada. Foram observados três exemplares de parasitos na cavidade abdominal, mas os rins encontravam-se preservados. Os parasitos foram fixados em formol acético e encaminhados ao laboratório de Parasitologia Veterinária da UFPR para identificação. Os exemplares coletados foram identificados como Dioctophyma renale, sendo duas fêmeas, uma com 39cm de comprimento por 4mm de largura e a outra com 16cm de comprimento por 4mm de largura e, um macho com 16cm de comprimento por 3mm de largura. O presente trabalho relata a ocorrência de parasitismo por D. renale em G. cuja na região oeste do estado do Paraná