2022: HIGH NOON OF POLITICAL TURBULANCE IN SLOVAKIA

The article examines the political processes in Slovakia in 2022. It is noted that the past year has demonstrated the fragility and weakness of the ruling coalition, which, following a lengthy debate, was forced to resign. The author notes that the central reason for the collapse of the Cabinet was the personal hostility of the country’s leading politicians – I. Matovič and R. Sulik. However, they demonstrated a keen desire to delay the collapse of the coalition, aggravating the internal political situation in the country as much as possible, since they realized the impossibility of participating in the formation of the next government following the results of early elections. The paper notes significant electoral changes in Slovakia since 2020: there has been a total decline in the popularity of the parties that formed the Cabinet, and an increase in the authority of the forces in opposition and promising the return of stable times. Meanwhile, early elections scheduled for September 30, 2023 do not yet guarantee the opposition a victory. The Slovak political tradition and the experience of neighboring Central European countries point to the possibility of liberal politicians uniting in various political alliances to prevent their ideological opponents from gaining powe

CZECH-UKRAINIAN RELATIONS AMIDST SPECIAL MILITARY OPERATION

The article outlines the main trends in Czech-Ukrainian relations since the start of the special military operation in Ukraine. The main events of the bilateral agenda between Prague and Kiev were considered. The author notes that after February 24, the current Cabinet under the leadership of P. Fiala began to perceive the Ukrainian issue as a key one in the country’s foreign policy. This was manifested in the increased intensity of contacts and a qualitative change in the content of the talks. An assumption is made about bringing relations to a strategic level. Moreover, it was noted that the Czech Republic sought to promote its vision of the Ukrainian issue among partners in international organizations through innovative proposals and playing ahead of the curve

Strength Analysis of Alternative Airframe Layouts of Regional Aircraft on the Basis of Automated Parametrical Models

This publication presents the results of complex parametrical strength investigations of typical wings for regional aircrafts obtained by means of the new version of the four-level algorithm (FLA) with the modified module responsible for the analysis of aerodynamic loading. This version of FLA, as well as a base one, is focused on significant decreasing time and labor input of a complex strength analysis of airframes by using simultaneously different principles of decomposition. The base version includes four-level decomposition of airframe and decomposition of strength tasks. The new one realizes additional decomposition of alternative variants of load cases during the process of determination of critical load cases. Such an algorithm is very suitable for strength analysis and designing airframes of regional aircrafts having a wide range of aerodynamic concepts. Results of validation of the new version of FLA for a high-aspect-ratio wing obtained in this work confirmed high performance of the algorithm in decreasing time and labor input of strength analysis of airframes at the preliminary stages of designing. During parametrical design investigation, some interesting results for strut-braced wings having high aspect ratios were obtained

Plankton biomass in the Mamala Basin (Oahu Island, Hawaii Islands) under antropogenic influence in August-September of 2002-2004

Anthropogenic impact on biomass of coastal plankton communities caused by submerged disposal of urban sewage waters (dumping) was studied. Observations were carried out in August-September of 2002-2004 in the Mamala Bay (Oahu Island, Hawaii Islands) using satellite and straight sea measurements. An analysis of variability of integral indicators of the water column determined on the basis of on-board measurements allowed us to divide them into two groups: elements most sensitive to pollution (heterotrophic bacteria (H-Bact), phototrophic cyanobacteria Synechococcus spp. (SYN), and chlorophyll a (CHLa)) and elements that manifested episodic positive dependence on inflow of polluted waters (heterotrophic unicellular eukaryotes, small unicellular algae, phototrophic green bacteria Prochlorococcus spp., as well as total biomass of microplankton). It was shown that submerged waste water disposal in the region of the diffuser of the dumping device led to insignificant (aver. 1.2-1.4 times) local increase in integral biomass of H-Bact, SYN, and in concentration of CHLa. Similar but sharper (aver. 1.5-2.1 times) increase in these parameters was found in water layers with maximal biomasses. Possible pathways of disposed waters (under the pycnocline, at its upper boundary, and in the entire mixed layer) were analyzed on the basis of studying vertical displacement of biomasses of H-Bact, SYN, and prochlorophytes. Possibility of using optical anomalies distinguished from satellite data as markers of anthropogenic eutrophication caused by dumping was confirmed. Application of such markers depends on water transparency and on shapes of curves of vertical distribution of autotrophic organisms

BK_Ca Activator NS1619 Improves the Structure and Function of Skeletal Muscle Mitochondria in Duchenne Dystrophy

Duchenne muscular dystrophy (DMD) is a progressive hereditary disease caused by the absence of the dystrophin protein. This is secondarily accompanied by a dysregulation of ion homeostasis, in which mitochondria play an important role. In the present work, we show that mitochondrial dysfunction in the skeletal muscles of dystrophin-deficient mdx mice is accompanied by a reduction in K+ transport and a decrease in its content in the matrix. This is associated with a decrease in the expression of the mitochondrial large-conductance calcium-activated potassium channel (mitoBKCa) in the muscles of mdx mice, which play an important role in cytoprotection. We observed that the BKCa activator NS1619 caused a normalization of mitoBKCa expression and potassium homeostasis in the muscle mitochondria of these animals, which was accompanied by an increase in the calcium retention capacity, mitigation of oxidative stress, and improvement in mitochondrial ultrastructure. This effect of NS1619 contributed to the reduction of degeneration/regeneration cycles and fibrosis in the skeletal muscles of mdx mice as well as a normalization of sarcomere size, but had no effect on the leakage of muscle enzymes and muscle strength loss. In the case of wild-type mice, we noted the negative effect of NS1619 manifested in the inhibition of the functional activity of mitochondria and disruption of their structure, which, however, did not significantly affect the state of the skeletal muscles of the animals. This article discusses the role of mitoBKCa in the development of DMD and the prospects of the approach associated with the correction of its function in treatments of this secondary channelopathy