A discrete-time performance model for congestion control mechanism using queue thresholds with QOS constraints

This paper presents a new analytical framework for the congestion control of Internet traffic using a queue threshold scheme. This framework includes two discrete-time analytical models for the performance evaluation of a threshold based congestion control mechanism and compares performance measurements through typical numerical results. To satisfy the low delay along with high throughput, model-I incorporates one threshold to make the arrival process step reduce from arrival rate ¿1 directly to ¿2 once the number of packets in the system has reached the threshold value L1. The source operates normally, otherwise. Model-II incorporates two thresholds to make the arrival rate linearly reduce from ¿1 to ¿2 with system contents when the number of packets in the system is between two thresholds L1 and L2. The source operates normally with arrival rate ¿1 before threshold L1, and with arrival rate ¿2 after the threshold L2. In both performance models, the mean packet delay W, probability of packet loss PL and throughput S have been found as functions of the thresholds and maximum drop probability. The performance comparison results for the two models have also been made through typical numerical results. The results clearly demonstrate how different load settings can provide different tradeoffs between throughput, loss probability and delay to suit different service requirements

Modifications of Myofilament Structure and Function During Global Myocardial Ischemia

Cardiac arrest is a prevalent condition with a poor prognosis, attributable in part to persistent myocardial dysfunction following resuscitation. The molecular basis of this dysfunction remains unclear. We induced cardiac arrest in a porcine model of acute sudden death and assessed the impact of ischemia and reperfusion on the molecular function of isolated cardiac contractile proteins. Cardiac arrest was electrically induced, left untreated for 12 min, and followed by a resuscitation protocol. With successful resuscitations, the heart was reperfused for 2 h (IR2) and the muscle harvested. In failed resuscitations, tissue samples were taken following the failed efforts (IDNR). Actin filament velocity, using myosin isolated from IR2 or IDNR cardiac tissue, was nearly identical to myosin from the control tissue in a motility assay. However, both maximal velocity (25% faster than control) and Ca2+ sensitivity (pCa50 6.57 ± 0.04 IDNR vs. 6.34 ± 0.07 control) were significantly (p \u3c 0.05) enhanced using native thin filaments (actin, troponin, and tropomyosin) from IDNR samples, suggesting that the enhanced velocity is mediated through an alteration in muscle regulatory proteins (troponin and tropomyosin). Mass spectrometry analysis showed that only samples from the IR2 had an increase in total phosphorylation levels of troponin (Tn) and tropomyosin (Tm), but both IR2 and IDNR samples demonstrated a significant shift from mono-phosphorylated to bis-phosphorylated forms of the inhibitory subunit of Tn (TnI) compared to control. This suggests that the shift to bis-phosphorylation of TnI is associated with the enhanced function in IDNR, but this effect may be attenuated when phosphorylation of Tm is increased in tandem, as was observed for IR2. There are likely many other molecular changes induced following cardiac arrest, but to our knowledge, these data provide the first evidence that this form cardiac arrest can alter the in vitro function of the cardiac contractile proteins

Performance modelling of a multiple threshold RED mechanism for bursty and correlated Internet traffic with MMPP arrival process

Access to the large web content hosted all over the world by users of the Internet engage many hosts, routers/switches and faster links. They challenge the internet backbone to operate at its capacity to assure e±cient content access. This may result in congestion and raises concerns over various Quality of Service (QoS) issues like high delays, high packet loss and low throughput of the system for various Internet applications. Thus, there is a need to develop effective congestion control mechanisms in order to meet various Quality of Service (QoS) related performance parameters. In this paper, our emphasis is on the Active Queue Management (AQM) mechanisms, particularly Random Early Detection (RED). We propose a threshold based novel analytical model based on standard RED mechanism. Various numerical examples are presented for Internet traffic scenarios containing both the burstiness and correlation properties of the network traffic

Acidosis and Phosphate Directly Reduce Myosin\u27s Force-Generating Capacity Through Distinct Molecular Mechanism

Elevated levels of the metabolic by-products, including acidosis (i.e., high [H+]) and phosphate (Pi) are putative agents of muscle fatigue; however, the mechanism through which they affect myosin’s function remain unclear. To elucidate these mechanisms, we directly examined the effect of acidosis (pH 6.5 vs. 7.4), alone and in combination with elevated levels of Pi on the force-generating capacity of a mini-ensemble of myosin using a laser trap assay. Acidosis decreased myosin’s average force-generating capacity by 20% (p \u3c 0.05). The reduction was due to both a decrease in the force generated during each actomyosin interaction, as well as an increase in the number of binding events generating negative forces. Adding Pi to the acidic condition resulted in a quantitatively similar decrease in force but was solely due to an elimination of all high force-generating events (\u3e2 pN), resulting from an acceleration of the myosin’s rate of detachment from actin. Acidosis and Pi also had distinct effects on myosin’s steady state ATPase rate with acidosis slowing it by ∼90% (p \u3e 0.05), while the addition of Piunder acidic conditions caused a significant recovery in the ATPase rate. These data suggest that these two fatigue agents have distinct effects on myosin’s cross-bridge cycle that may underlie the synergistic effect that they have muscle force. Thus these data provide novel molecular insight into the mechanisms underlying the depressive effects of Pi and H+ on muscle contraction during fatigue

Why Penetration Testing is a Limited Use Choice for Sound Cyber Security Practice

Penetration testing of networks is a process that is overused when demonstrating or evaluating the cyber security posture of an organisation. Most penetration testing is not aligned with the actual intent of the testing, but rather is driven by a management directive of wanting to be seen to be addressing the issue of cyber security. The use of penetration testing is commonly a reaction to an adverse audit outcome or as a result of being penetrated in the first place. Penetration testing used in this fashion delivers little or no value to the organisation being tested for a number of reasons. First, a test is only as good as the tools, the tester and the methodology being applied. Second, the results are largely temporal. That is, the test will likely only find known vulnerabilities that exist at one specific point in time and not larger longitudinal flaws with the cyber security of an organisation, one such flaw commonly being governance. Finally, in many cases, one has to question what the point is in breaking the already broken. Penetration testing has its place when used judiciously and as part of an overall review and audit of cyber security. It can be an invaluable tool to assess the ability of a system to survive a sustained attack if properly scoped and deployed. However, it is our assessment and judgement that this rarely occurs. Keywords: cyber security, penetration testing, vulnerability assessmen

An Electrolysis Experiment for a Middle School Summer Science Camp

Higher education is often culturally deemphasized in the geographic area served by our rural, regional campus. As a result, faculty members have the opportunity to spearhead teaching efforts designed to educate the community about the importance of obtaining a post-secondary degree. To this end, we recently held a Science Summer Camp for middle school students, designed to infuse young people with an increased excitement for STEM (Science, Technology, Engineering, and Math) education. In this report, we summarize a chemical electrolysis experiment we carried out with middle school students for our annual Science Summer Camp. We also provided procedural guidelines for small- and large-scale experiments. In the latter case, evolved H2 gas can be detonated for effect. Two modifications from literature procedure include: (1) using glass burettes, instead of test tubes, to collect the evolving H2 and O2 gases for the small-scale setup; and (2) prefilling the 100-mL graduated collection cylinders with aqueous NaOH prior to beginning electrolysis. Because these modifications provide aqueous solution in the collection reservoirs prior to starting the experiment, the total time required for the experiment is greatly reduced (~30 minutes)

Direct Observation of Phosphate Inhibiting the Force-Generating Capacity of a Miniensemble of Myosin Molecules

AbstractElevated levels of phosphate (Pi) reduce isometric force, providing support for the notion that the release of Pi from myosin is closely associated with the generation of muscular force. Pi is thought to rebind to actomyosin in an ADP-bound state and reverse the force-generating steps, including the rotation of the lever arm (i.e., the powerstroke). Despite extensive study, this mechanism remains controversial, in part because it fails to explain the effects of Pi on isometric ATPase and unloaded shortening velocity. To gain new insight into this process, we determined the effect of Pi on the force-generating capacity of a small ensemble of myosin (∼12 myosin heads) using a three-bead laser trap assay. In the absence of Pi, myosin pulled the actin filament out of the laser trap an average distance of 54 ± 4 nm, translating into an average peak force of 1.2 pN. By contrast, in the presence of 30 mM Pi, myosin generated only enough force to displace the actin filament by 13 ± 1 nm, generating just 0.2 pN of force. The elevated Pi also caused a >65% reduction in binding-event lifetime, suggesting that Pi induces premature detachment from a strongly bound state. Definitive evidence of a Pi-induced powerstroke reversal was not observed, therefore we determined if a branched kinetic model in which Pi induces detachment from a strongly bound, postpowerstroke state could explain these observations. The model was able to accurately reproduce not only the data presented here, but also the effects of Pi on both isometric ATPase in muscle fibers and actin filament velocity in a motility assay. The ability of the model to capture the findings presented here as well as previous findings suggests that Pi-induced inhibition of force may proceed along a kinetic pathway different from that of force generation

Acidosis and Phosphate Directly Reduce Myosin’s Force-Generating Capacity Through Distinct Molecular Mechanisms

Elevated levels of the metabolic by-products, including acidosis (i.e., high [H+]) and phosphate (Pi) are putative agents of muscle fatigue; however, the mechanism through which they affect myosin’s function remain unclear. To elucidate these mechanisms, we directly examined the effect of acidosis (pH 6.5 vs. 7.4), alone and in combination with elevated levels of Pi on the force-generating capacity of a mini-ensemble of myosin using a laser trap assay. Acidosis decreased myosin’s average force-generating capacity by 20% (p < 0.05). The reduction was due to both a decrease in the force generated during each actomyosin interaction, as well as an increase in the number of binding events generating negative forces. Adding Pi to the acidic condition resulted in a quantitatively similar decrease in force but was solely due to an elimination of all high force-generating events (>2 pN), resulting from an acceleration of the myosin’s rate of detachment from actin. Acidosis and Pi also had distinct effects on myosin’s steady state ATPase rate with acidosis slowing it by ∼90% (p > 0.05), while the addition of Pi under acidic conditions caused a significant recovery in the ATPase rate. These data suggest that these two fatigue agents have distinct effects on myosin’s cross-bridge cycle that may underlie the synergistic effect that they have muscle force. Thus these data provide novel molecular insight into the mechanisms underlying the depressive effects of Pi and H+ on muscle contraction during fatigue

Reviews

Reviews of Business and New Zealand Society, Women in Trade Unions: Organizing the Unorganized, Labour Law and Industrial Relations in Asia, International and Comparative Industrial Relations: A Study of Industrialised Market Economies, The Challenge of Human Resource Management Directions and Debates in New Zealand, Visions of the Future of Social Justice: Essays on the Occasion of the ILO's 75th Aniversary, Coal, Class and Community: The United Mineworkers of New Zealand, 1880-1960, Higher Productivity and a Better Place to Work - Practical Ideas (or Owners and Managers of Small and Medium-Sized Enterprises, OECD Societies in Transition." The Future of Wo.rk and Leisure