One hundred of cells generations connect the resistance to drugs and the replicative aging mechanisms

There are researchs that reveal a relationship between acquired resistance to antineoplastic drugs and aging. Therefore, the identification of genes involved in senescence could have a potential therapeutic utility for the prediction and/or prevention of resistance to antineoplastic drugs. The aim of this work is to study the relationship between replicative aging and acquired resistance to Cisplatin and Bleomycin in the yeast Saccharomyces cerevisiae.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Proteomic analysis in wild type and cisplatin resistant yeast strain of saccharomyces cerevisiae

Research carried out in oncology has allowed the identification and characterization of molecular markers of resistance to antineoplastic drugs. It supposes a great advance and allows the administration of customized chemotherapies to patients. The aim of this work is to carry out a proteomic analysis in the wild type strain and cisplatin resistant strain of Saccharomyces cerevisiae in order to look for possible markers of resistance, as well as the mechanisms involved.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Analysis of strain localization with a nonlocal plasticity model

In the present paper a nonlocal plasticity model is described, intended to reproduce the mechanical behaviour of stiff fine-grained soils, including the objective simulation of strain localization; the phenomenon of accumulation of deformations in narrow zones in the form of shear bands or fractures. A number of analyses have been performed to assess the developed formulation. Relevant aspects have been addressed such as the thickness of the shear band, its orientation, and the onset of localization in a boundary value problem (BVP). Results provide useful insigths into relevant aspects of the numerical simulation of strain localization

Mechanisms of cross resistance between cisplatin and bleomycin in saccharomyces cerevisiae

Tumor cells can simultaneously acquire resistance against a diverse group of drugs. This phenomenon is called pleotropic resistance or multidrug resistance. This mechanism could explain the tumor resistance observed in chemotherapy treatments with multiple agents. The aim of this work is to study possible mechanisms of cross resistance between Cisplatin and Bleomycin in Saccharomyces cerevisiae.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

New markers of bleomycin resistance

Resistance to chemotherapy is one of the main problems of this type of therapy. Studies carried out determine the usefulness of predictive biomarkers to improve this treatment. The aim of this work is to carry out a proteomic analysis in the wild type strain and bleomycin resistant strain of Saccharomyces cerevisiae in order to look for possible markers of resistance, as well as the mechanisms involved.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

A time-dependent anisotropic model for argillaceous rocks: application to an underground excavation in Callovo-Oxfordian claystone

The paper presents a constitutive model for argillaceous rocks, developed within the framework of elastoplasticity, that includes a number of features that are relevant for a satisfactory description of their hydromechanical behaviour: anisotropy of strength and stiffness, behaviour nonlinearity and occurrence of plastic strains prior to peak strength, significant softening after peak, time-dependent creep deformations and permeability increase due to damage. Both saturated and unsaturated conditions are envisaged. The constitutive model is then applied to the simulation of triaxial and creep tests on Callovo-Oxfordian (COx) claystone. Although the main objective has been the simulation of the COx claystone behaviour, the model can be readily used for other argillaceous materials. The constitutive model developed is then applied, via a suitable coupled hydromechanical formulation, to the analysis of the excavation of a drift in the Meuse/Haute-Marne Underground Research Laboratory. The pattern of observed pore water pressures and displacements, as well as the shape and extent of the damaged zone, are generally satisfactorily reproduced. The relevance and importance of rock anisotropy and of the development of a damaged zone around the excavations are clearly demonstrated.Peer ReviewedPostprint (author's final draft

Propuesta de un plan de mantenimiento preventivo para la caldera de diésel York Shipley instalada en Planta de sub-producto COESA, de Avícola La Estrella, S.A, en el periodo comprendido de agosto a noviembre, 2015

El presente trabajo se realizó en el área de calderas de la Planta de Sub-producto COESA de Avícola La Estrella, S.A., ubicada en el Km 29 ½ de la carretera norte. Esta pequeña planta comenzó operaciones a mediados del mes de mayo de presente año. En la planta se realiza el proceso de elaboración de harina de pollo de todas las aves que son desechadas en las galeras de la empresa. En el inicio del presente trabajo se plantearon los objetivos (general y específicos) cuyo propósito es la mayor optimización productiva del equipo, de la misma manera en el planteamiento del problema se expone que los paros repentinos de la máquina son el principal problema por la falta de un adecuado mantenimiento. En cuanto al marco referencial se establecieron las respectivas teorías, definiciones, las especificaciones de las leyes con las que regir el presente estudio, la localización de la empresa y la especificación temporal en la que se realizó. Este estudio tiene un enfoque de carácter mixto ya que se describen y analizan los datos que generan la información encontrada de la máquina. De la misma manera se estableció el universo, población y muestra que corresponde a la empresa, la planta de subproducto y la máquina evaluada respectivamente y luego las herramientas de investigación que se utilizaron como entrevista, observación y métodos como el árbol de problemas y de fallos y la metodología HAZOP. En el desarrollo se caracteriza el proceso de producción por medio del diagrama de flujo de la norma ISO, se analizan las fallas con el árbol de problemas y árbol de fallo, se implementa la metodología HAZOP en donde se encuentra la “presión” como el mayor factor de riesgo y finalmente la aplicación del correspondiente “Manual MPP” para la caldera con un breve análisis de los costos de mantenimiento, los resultados de la entrevista y la observación aplicada. Finalmente la conclusión basada en los objetivos que se establecieron y luego las respectivas recomendaciones de mejoras para la planta y la caldera en estudio

Resistance to bleomycin increases the chronological life of cells

The identification of genes involved in chronological aging could have a potential utility as molecular markers in the chemotherapy treatment of cancer. The aim of this work is to study the relationship between the chronological aging and the resistance to Bleomycin for the purpose of establish the basic interactions between these phenomenons for further investigation of molecular markers.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Resistance acquisition after seven cycles of in vitro bleomycin exposure

Bleomycin is an antineoplastic drug that causes DNA damage by intra- and inter-strand crosslink and DNA strand breaks. This drug is used to treat several types of cancer. The aim of this work is to study the effect of Bleomycin on S. cerevisiae cells and select bleomycin-resistant cells to study the process of resistance acquisition to establish new protocols in in vitro drugs exposure.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Relationship between chronological aging and acquired resistance to cisplatin in the yeast saccharomyces cerevisiae

The Sir2 gene is associated with an increase in longevity in yeasts, worms, flies and rodents. The human homolog, Sirt1, is also involved in longevity, by inhibiting cellular senescence. Studies carry out in tumor cells of neuroblastoma, osteosarcoma, breast and ovary, resistant and sensitive to antineoplastic drugs, suggest that the increase of Sirt1 expression may represent a general phenomenon associated with resistance to chemotherapy, independent of cell type or drug used to induce resistance. Thus, after analyzing tumor biopsies after treating patients with chemotherapy, high amounts of Sirt1 have been observed. The aim of this work is to study the relationship between chronological aging and acquired resistance to Cisplatin in the yeast Saccharomyces cerevisiae.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech