Complex oscillatory redox dynamics with signaling potential at the edge between normal and pathological mitochondrial function

The time-keeping properties bestowed by oscillatory behavior on functional rhythms represent an evolutionarily conserved trait in living systems. Mitochondrial networks function as timekeepers maximizing energetic output while tuning reactive oxygen species (ROS) within physiological levels compatible with signaling. In this work, we explore the potential for timekeeping functions dependent on mitochondrial dynamics with the validated two-compartment mitochondrial energetic-redox (ME-R) computational model, that takes into account (a) four main redox couples [NADH, NADPH, GSH, Trx(SH)2], (b) scavenging systems (glutathione, thioredoxin, SOD, catalase) distributed in matrix and extra-matrix compartments, and (c) transport of ROS species between them. Herein, we describe that the ME-R model can exhibit highly complex oscillatory dynamics in energetic/redox variables and ROS species, consisting of at least five frequencies with modulated amplitudes and period according to power spectral analysis. By stability analysis we describe that the extent of steady state—as against complex oscillatory behavior—was dependent upon the abundance of Mn and Cu, Zn SODs, and their interplay with ROS production in the respiratory chain. Large parametric regions corresponding to oscillatory dynamics of increasingly complex waveforms were obtained at low Cu, Zn SOD concentration as a function of Mn SOD. This oscillatory domain was greatly reduced at higher levels of Cu, Zn SOD. Interestingly, the realm of complex oscillations was located at the edge between normal and pathological mitochondrial energetic behavior, and was characterized by oxidative stress. We conclude that complex oscillatory dynamics could represent a frequency- and amplitude-modulated H2O2 signaling mechanism that arises under intense oxidative stress. By modulating SOD, cells could have evolved an adaptive compromise between relative constancy and the flexibility required under stressful redox/energetic conditions.Fil: Kembro, Jackelyn Melissa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Cortassa, Sonia del Carmen. University Johns Hopkins; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aon, Miguel A.. University Johns Hopkins; Estados Unido

Collaborative Development within Open Source Communities

Open source communities are one of the most successful-- and least appreciated--examples of high-performance collaboration and community building on the Internet today. Open source communities began as loosely organized, ad-hoc communities of contributors from all over the world who shared an interest in meeting a common need. However, the organization of these communities has proven to be very flexible and capable of carrying out all kind of developments, ranging from minor projects to huge programs such as Apache (Höhn, & Herr, 2004; Mockus, Fielding, & Herbsleb, 2005

Decomposition of state-space Model with inputs: The theory and an application to estimate the ROI of advertising

This paper shows how to compute the in-sample effect of exogenous inputs on the endogenous variables in any linear model written in state-space form. Estimating this component may be, either interesting by itself, or a previous step before decomposing a time series into trend, cycle, seasonal and error components. The practical application and usefulness of this method is illustrated by estimating the effect of advertising on monthly sales of the Lydia Pinkham vegetable compound.State-space, Signal extraction, Time series decomposition, Seasonal adjustment, Advertising, Lydia Pinkham

A proposal to obtain a long quarterly chilean gdp series

An important limitation in order to specify and estimate a macroeconomic model that describes the Chilean economy resides in using variables with sufficient number of observations that allow for a reliable econometric estimation. Among these variables, the GDP constitutes a fundamental magnitude. Nevertheless, for this variable there is not quarterly information before 1980. This paper computes quarterly GDP series for the period 1966-1979 using the approach by Casals et al (2000). As result, the new series incorporates the cyclical dynamic in the quarterly series later to 1979 respecting, in addition, all the annual existing information before the above mentioned period

Estudio taxonómico y filogenético de las variedades de Struthiopteris spicant (L.) Weiss (Blechnaceae, Polypodiopsida) en Europa

Struthiopteris spicant (L.) Weiss es un helecho con una amplia distribución en el hemisferio norte, en América y Eurasia. Presenta cierta plasticidad fenotípica, de manera que algunas entidades subordinadas han sido descritas, siendo consideradas la mayoría variedades y formas ecológicas. Dos de estas variedades aparecen en la Península Ibérica, la más común, la variedad spicant, y la variedad homophyllum (Merino) Gabriel y Galán & Pino, que está definida como endémica del noreste de la Península. Además, varios individuos con una apariencia similar y difícilmente asignables a ninguna de las variedades descritas han sido encontrados en el norte de la Península, Salamanca y las Islas Canarias. También existe una variedad islandesa, la variedad fallax (Lange) Wasowicz & Gabriel y Galán, endémica de Deildartunguhver (oeste de Islandia), a la que hemos tenido acceso. Todas estas formas presentan diferencias morfológicas muy notables que nos hacen dudar de su rango taxonómico. En este trabajo, se ha llevado a cabo un análisis morfológico y anatómico completo de 43 individuos de las tres formas. Además, en 15 individuos se ha realizado un estudio genético de sitios polimórficos utilizando marcadores cloroplásticos, nucleares y microsatélites. Los resultados obtenidos indican que algunos caracteres anatómicos (sobre todo las escamas y los tamaños foliares) podrían tener una significación taxonómica, lo que viene apoyado por los datos moleculares. En conclusión, se puede considerar que estamos tratando con entes de rango taxonómico mayor que meras variedades

Sinopsis global y aproximación a la filogenia molecular del género Parablechnum C. Presl (Blechnaceae, Polypodiopsida)

Tesis inédita de la Universidad Complutense de Madrid, Facultad de Ciencias Biológicas, leída el 05-09-2022The family Blechnaceae is a lineage that includes about 280 species in 25 genera, and together with the Onocleaceae is the most recent family of ferns. Within this family, Parablechnum is the most diverse genus, with about 65 species. The distribution of this genus reflects very well the distribution of the most diverse areas of the family: Central and South America, the Austropacific and some representatives in southern Africa, Madagascar and the Mascarene archipelago.The distribution of this genus reflects a disjunction that could easily be explained by Gondwanan vicariance, but the available dating indicates that the genus underwent a rapid radiation after the separation from Gondwana, which would imply that its distribution is due to long-distance dispersal (LDD). In addition, the intra-continental dispersal of the genus together with other problems such as poor representation in herbaria has made the taxonomy of Parablechnum unclear to date.In this context, a revision of the systematics of the genus has been proposed using herbarium material and some field collections to elucidate the taxonomy and evolutionary history of the genus.For this purpose, 3,738 specimens from 52 herbariums of all the species of Parablechnum conceived to date have been examined physically and digitally. Where possible, the anatomy of the fertile pinnae and epidermis was studied, and spores and sporangia were observed by light and electron microscopy. In addition, DNA was extracted from 127 individuals and five chloroplastic markers were sequenced for maximum likelihood phylogenetic trees and Bayesian inference. In addition, a dating have been carried out. For this purpose, 582 sequences were used, 469 of which were newly created in this work...La familia Blechnaceae es un linaje que incluye unas 280 especies en 25 géneros, y junto con las Onocleaceae es la familia más reciente de helechos. Dentro de esta familia, Parablechnum es el género más diverso, con alrededor de 65 especies. La distribución de este género refleja muy bien la distribución de los puntos más diversos de la familia: América Central y del Sur, el Austropacífico y algunos representantes en el sur de África, Madagascar y el archipiélago mascareno. La distribución de este género refleja una disyunción que podría explicarse fácilmente por la vicarianza gondwánica, pero las dataciones disponibles indican que el género tuvo una radiación rápida tras la separación de Gondwana, lo que implicaría que su distribución se debe a la dispersión a larga distancia (LDD). Además, la dispersión dentro de los distintos continentes del género junto con otros problemas como la escasa representación en los herbarios ha hecho que la taxonomía de Parablechnum no esté clara hasta la fecha. En este contexto, se ha propuesto una revisión de la sistemática del género utilizando material de herbario y algunas colecciones de campo para dilucidar la taxonomía y la historia evolutiva del género. Para ello, se han examinado 3.738 pliegos provenientes de 52 herbarios de todas las especies concebidas hasta hoy de Parablechnum, de forma física y presencial. En los taxones en los que ha sido posible se ha estudiado de la anatomía de las pinnas fértiles, la epidermis y se han observado las esporas y los esporangios con microscopía óptica y electrónica. Así mismo, se ha extraído ADN de 127 individuos y se han secuenciado cinco marcadores cloroplásticos para la realización de árboles filogenéticos de máxima verosimilitud e inferencia bayesiana. Además, se ha realizado una datación. Para este fin se han utilizado 582 secuencias de las cuales 469 son de nueva creación en este trabajo...Fac. de Ciencias BiológicasTRUEunpu

A PROPOSAL TO OBTAIN A LONG QUARTERLY CHILEAN GDP SERIES

An important limitation in order to specify and estimate a macroeconomic model that describes the Chilean economy resides in using variables with sufficient number of observations that allow for a reliable econometric estimation. Among these variables, the GDP constitutes a fundamental magnitude. Nevertheless, for this variable there is not quarterly information before 1980. This paper computes quarterly GDP series for the period 1966-1979 using the approach by Casals et al (2000). As result, the new series incorporates the cyclical dynamic in the quarterly series later to 1979 respecting, in addition, all the annual existing information before the above mentioned period.

A Proposal to Obtain a Long Quarterly Chilean GDP Series

An important limitation in order to specify and estimate a macroeconomic model that describes the Chilean economy resides in using variables with sufficient number of observations that allow for a reliable econometric estimation. Among these variables, thSmoothing algorithm, arima model, transfer function model, chilean gdp

Synthesis of a Structured Material Based on Compact Spheres Coated with Zn or Mg Spinel

In this work, a synthesis method of coating with thin layers of MgAl2O4 or ZnAl2O4 over alpha-Al2O3 spheres were developed.The method consisted in the deposition of a primer of bohemite on the spheres, followed by the impregnation with a solution of Mg or Zn nitrates, and further thermal treatments in order to obtain MAl2O4 (M: Mg or Zn). This method was modified to improve the thickness and purity of the layer material. With this method, layers of uniform and adequate thickness and good adhesion were achieved. Pt catalysts prepared with these materials as supports displayed good catalytic performances in the n-butanedehydrogenation reaction, especially those obtained with purified supports.Fil: Bocanegra, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; ArgentinaFil: Ballarini, Adriana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; ArgentinaFil: Scelza, Osvaldo Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; ArgentinaFil: de Miguel, Sergio Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; Argentin