7 research outputs found
Whole-body cryostimulation impact on blood serum thermal denaturation profiles of cross-country skiers
Blood plasma and serum are important diagnostic materials as they clearly reflect an individual’s metabolism. The study
has evaluated the effect of whole-body cryostimulation (WBC) on the blood serum of professional cross-country skiers.
The experiment involved eight athletes (two women and six men) who underwent a series of ten WBC treatments. Aqueous
solutions of human blood serum samples before WBC procedures, after one treatment and after a series of ten treatments
were measured by means of differential scanning calorimetry (DSC), a relatively novel diagnostic tool. DSC results
showed rather little impact of cryostimulation on heat capacity changes accompanying the process of thermal denaturation
of blood serum proteins in elite athletes. However, the statistically significant reduction in the intensity of the serum
denaturation transition in its low temperature range has been observed after ten WBC treatments. The results have been
interpreted by changes in the serum proteome profile, notably in the ratio of ligated to unligated albumin molecules. As a
side result, the relationships between the relative change in body fat mass after ten WBC treatments and the levels of
alpha2-globulins and beta2-globulins fractions have been found
Comparison of human blood serum DSC profiles in aqueous and PBS buffer solutions
The results of studies of physiological fluids by differential scanning calorimetry (DSC) for the purpose of diagnosis and monitoring of diseases are promising. Before the DSC method is accepted in medical diagnostics, it is worth experimenting with various environmental conditions at the research stage. Among other things, it is important to choose an appropriate solvent to dilute the tested biological fluids. In this work, human blood sera DSC profiles in aqueous and PBS (phosphatebuffered saline) solutions have been compared. Visibility of haptoglobin in the DSC profile of human blood serum is much better in water solution. In addition, contributions from albumin and haptoglobin are well separated in contrast to the PBS serum solutions. The peak or shoulder at about 83 °C which represents contributions from the CH3 domain of immunoglobulin
IgG1 and/or transferrin is more clearly visible in PBS solution. The results show that the type of solvent is essential when interpreting the serum DSC profile
Blood serum denaturation profile examined by differential scanning calorimetry reflects the effort put into ultramarathon by amateur long-distance runners
The impact of participation in the ultramarathon on the health and mental and physical condition is very
complex. Undoubtedly, exercise brings many benefits but also involves health risks. Especially such an extreme
effort as the one associated with finishing the ultramarathon run, can be dangerous to the health of the runner.
With the variety of possible biomarkers of excessive fatigue that threaten health and life, a question arises which
of them are the best and which should be considered in amateur long-distance runners showing particularly high
individual variability. In this study differential scanning calorimetry (DSC) has been applied to show the overall
effect of the 12-h run on blood sera of participants. Serum samples were obtained from the blood of ten male
amateur long-distance runners, collected before and immediately after the run. Distinct changes in the shape of
DSC curves have been observed for serum after finishing the run relative to pre-race serum. Statistically significant
differences between stages “before” and “after” ultramarathon running have been found for parameters
of the endothermic transition associated with denaturation of serum proteins. An increase in the temperature
(from 70.9 ± 0.9 to 75.8 ± 2.9 ◦C) and excess heat capacity (from 0.859 ± 0.201 to 1.102 ± 0.226 Jg-1 ◦C-1) at
peak maximum, the enthalpy of serum denaturation (from 18.55 ± 6.52 to 22.08 ± 5.61 Jg-1) and the first
moment of the thermal transition with respect to the temperature (from the value of 67.0 ± 2.1 to 72.6 ± 2.1 ◦C)
has been observed. These results show a clear impact of running an ultramarathon on the participant’s blood
serum
"Stare" i "nowe" media w kontekście kampanii politycznych i sprawowania władzy
Praca recenzowana / peer-reviewed paperPublikacja składa się z piętnastu tekstów autorów reprezentujących ważne ośrodki akademickie kraju. Podzielona została na dwie części. Pierwszą stanowią teksty ukazujące wieloaspektowość funkcjonowania mediów w przestrzeni publicznej. Druga część publikacji to zróżnicowane przykłady zastosowania nowoczesnych mediów w funkcjonowaniu współczesnej polityki światowej i krajowej