Recall Of Informed Consent For Prenatal Aneuploidy Screening

Several forms of prenatal screening and diagnostic testing are available that can provide information about the likelihood of a genetic or chromosomal condition in pregnancy. Each of the available technologies entail unique benefits and limitations, and patient comprehension of the differences among these tests is crucial to uphold the principle of informed consent. The primary research goal of our study was to establish what women recall of the benefits, risks, and limitations of the prenatal aneuploidy screening they were offered as a part of their prenatal care by their medical provider. A total of 349 women were surveyed and 182 met eligibility criteria having had a recent or third-trimester pregnancy. Overall knowledge scores for participants ranged from 0 to 82.9 out of 100, with an average score of 32.8 (SD = 21.9). We found significantly higher scores in women who were offered testing by genetic professionals, met with a genetic counselor in prior pregnancies, were 35-years or older, or were given the choice of both screening and diagnostic tests. Our data support the importance of a thorough informed consent when discussing prenatal aneuploidy testing with patients

Biomarkers of Heavy Metal Effects in Two Species of Caddisfly Larvae from Clark Fork River, Montana: Stress Proteins (HSP70) and Lysosomal Membrane Integrity

Potential sublethal effects of heavy metals in stream macroinvertebrates were examined with two cellular and biochemical biomarkers in larvae of two caddisflies indigenous to the Clark Fork River, Montana, - Hydropsyche spp. and Arctopsyche grandis. Stress proteins, in particular members of the HSP70 family, are involved in cellular protein homeostasis and repair, and are induced by a variety of stressors, which either damage cellular proteins directly or cause cells to synthesize aberrant proteins. Lysosomes are intracellular organelles that play key roles in the detoxification of both organic and inorganic xenobiotic compounds. Larvae of Hydropsyche spp. were collected from four sites on the Clark Fork (Galen Gage--4.7 km, Goldcreek--85.6 km, Turah--189.7 km, above Flathead--381 km) and a reference site (the Blackfoot River). Larvae of A. grandis were collected from the same sites minus the Galen site. Samples were immediately frozen in liquid nitrogen for HSP70 analysis, or preserved with Tissue Tek, then frozen in liquid nitrogen for the lysosomal stability assay. HSP70 was analyzed by western blotting using monoclonal antibodies. Lysosomal integrity was measured in cryosections by acid labilization with acid phosphatase as a marker enzyme. Results to date show elevated tissue concentrations of Cd, Cu, Pb and Zn and significantly increased levels of HSP70 in Arctopsyche from Goldcreek compared to reference samples. Lysosomal integrity also was compromised in samples from Goldcreek. In Hydropsyche, tissue concentrations of Cd, Cu and Pb from Galen Gage were elevated (4-7 times) relative to the Blackfoot River, but levels of HSP70 did not differ between the two sites. These preliminary results indicate that sublethal effects of metal exposure may differ between species

Further improvement of warming-equivalent emissions calculation

GWP* was recently proposed1 as a simple metric for calculating warming-equivalent emissions by equating a change in the rate of emission of a short-lived climate pollutant (SLCP) to a pulse emission of carbon dioxide. Other metrics aiming to account for the time-dependent impact of SLCP emissions, such as CGWP, have also been proposed2. In 2019 an improvement to GWP* was proposed by Cain et al.3, hereafter CLA, combining both the rate and change in rate of SLCP emission, justified by the rate of forcing decline required to stabilise temperatures following a recent multi-decade emissions increase. Here we provide a more direct justification of the coefficients used in this definition of GWP*, with a small revision to their absolute values, by equating CO2 and SLCP forcing directly, without reference to the temperature response. This provides a more direct link to the impulse-response model used to calculate GWP values and improves consistency with CGWP values

Agriculture's contribution to climate change and role in mitigation is distinct from predominantly fossil CO2-emitting sectors

Agriculture is a significant contributor to anthropogenic global warming, and reducing agricultural emissions—largely methane and nitrous oxide—could play a significant role in climate change mitigation. However, there are important differences between carbon dioxide (CO2), which is a stock pollutant, and methane (CH4), which is predominantly a flow pollutant. These dynamics mean that conventional reporting of aggregated CO2-equivalent emission rates is highly ambiguous and does not straightforwardly reflect historical or anticipated contributions to global temperature change. As a result, the roles and responsibilities of different sectors emitting different gases are similarly obscured by the common means of communicating emission reduction scenarios using CO2-equivalence. We argue for a shift in how we report agricultural greenhouse gas emissions and think about their mitigation to better reflect the distinct roles of different greenhouse gases. Policy-makers, stakeholders, and society at large should also be reminded that the role of agriculture in climate mitigation is a much broader topic than climate science alone can inform, including considerations of economic and technical feasibility, preferences for food supply and land-use, and notions of fairness and justice. A more nuanced perspective on the impacts of different emissions could aid these conversation

Co-binding by YY1 identifies the transcriptionally active, highly conserved set of CTCF-bound regions in primate genomes.

BACKGROUND: The genomic binding of CTCF is highly conserved across mammals, but the mechanisms that underlie its stability are poorly understood. One transcription factor known to functionally interact with CTCF in the context of X-chromosome inactivation is the ubiquitously expressed YY1. Because combinatorial transcription factor binding can contribute to the evolutionary stabilization of regulatory regions, we tested whether YY1 and CTCF co-binding could in part account for conservation of CTCF binding. RESULTS: Combined analysis of CTCF and YY1 binding in lymphoblastoid cell lines from seven primates, as well as in mouse and human livers, reveals extensive genome-wide co-localization specifically at evolutionarily stable CTCF-bound regions. CTCF-YY1 co-bound regions resemble regions bound by YY1 alone, as they enrich for active histone marks, RNA polymerase II and transcription factor binding. Although these highly conserved, transcriptionally active CTCF-YY1 co-bound regions are often promoter-proximal, gene-distal regions show similar molecular features. CONCLUSIONS: Our results reveal that these two ubiquitously expressed, multi-functional zinc-finger proteins collaborate in functionally active regions to stabilize one another's genome-wide binding across primate evolution

Quantifying non-CO2 contributions to remaining carbon budgets

The IPCC Special Report on 1.5 °C concluded that anthropogenic global warming is determined by cumulative anthropogenic CO2 emissions and the non-CO2 radiative forcing level in the decades prior to peak warming. We quantify this using CO2-forcing-equivalent (CO2-fe) emissions. We produce an observationally constrained estimate of the Transient Climate Response to cumulative carbon Emissions (TCRE), giving a 90% confidence interval of 0.26–0.78 °C/TtCO2, implying a remaining total CO2-fe budget from 2020 to 1.5 °C of 350–1040 GtCO2-fe, where non-CO2 forcing changes take up 50 to 300 GtCO2-fe. Using a central non-CO2 forcing estimate, the remaining CO2 budgets are 640, 545, 455 GtCO2 for a 33, 50 or 66% chance of limiting warming to 1.5 °C. We discuss the impact of GMST revisions and the contribution of non-CO2 mitigation to remaining budgets, determining that reporting budgets in CO2-fe for alternative definitions of GMST, displaying CO2 and non-CO2 contributions using a two-dimensional presentation, offers the most transparent approach

Searching for Novel Biomarkers Using a Mouse Model of CLN3-Batten Disease

CLN3-Batten disease is a rare, autosomal recessive disorder involving seizures, visual, motor and cognitive decline, and premature death. The Cln3Δex7/8 mouse model recapitulates several phenotypic characteristics of the most common 1.02kb disease-associated deletion. Identification of reproducible biomarker(s) to facilitate longitudinal monitoring of disease progression and provide readouts for therapeutic response has remained elusive. One factor that has complicated the identification of suitable biomarkers in this mouse model has been that variations in animal husbandry appear to significantly influence readouts. In the current study, we cross-compared a number of biological parameters in blood from Cln3Δex7/8 mice and control, non-disease mice on the same genetic background from multiple animal facilities in an attempt to better define a surrogate marker of CLN3-Batten disease. Interestingly, we found that significant differences between Batten and non-disease mice found at one site were generally not maintained across different facilities. Our results suggest that colony variation in the Cln3Δex7/8 mouse model of CLN3-Batten disease can influence potential biomarkers of the disease

Habitat structure is linked to the evolution of plumage colour in female, but not male, fairy-wrens

Background Both natural and sexual selection may drive the evolution of plumage colouration in birds. This can lead to great variation in plumage not only across species, but also between sexes within species. Australasian fairy-wrens are famous for their brightly coloured males, which exhibit colours ranging from bright blue to red and black. Female plumage in fairy wrens (and in general) has been rarely studied, but it can also be highly variable, including both bright and cryptic plumages. We use a comparative framework to explore the basis for this variation, and test the possibility that female fairy-wrens experience selection for cryptic plumage when they occupy more exposed habitats that offer little concealment from predators. We use spectral measurements of plumage for species and subspecies of Australasian fairy-wrens. Results We show that female colouration (contrast against background) is strongly correlated with vegetation cover: females in open habitats show less contrast to background colours than females in closed habitats, while male colouration is not associated with habitat type. Conclusions Female plumage appears to be under stronger natural selection than male plumage in fairy-wrens, providing an example of how selection may act differently on males and females of the same species

Is the digital rectal exam any good as a prostate cancer screening test?

© The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/There is no shortage of references in popular culture to the prostate examination, with many a laugh built on the punchline of the finger up the bum. Interestingly, while cervical, breast, or bowel screening share barriers to uptake around the intimacy of the examination, ‘ick-factor’, or cultural taboos, they have never become comedy tropes — reflecting the uniquely emasculating perception of the rectal examination.Peer reviewe

Chemical Reaction Dynamics at Surfaces

Contains reports on two research projects with publication lists in each section.Joint Services Electronics Program Contract DAAL03-89-C-0001MIT Energy Laboratory Synthetic Fuels CenterNational Science Foundation Grant CHE 85-08734Petroleum Research Fund Contract 19014-AC