The survival of the optative in New Testament Greek

This article offers new insights on the status of the optative in New Testament Greek, mapping it against the diachronic encoding of modality in Ancient Greek in light of typology and pragmatics. Virtually all available scholarship on the subject focusses on the ‘decline’ of the optative; in this article, we choose to focus on its survival in fixed expressions and specific types of speech acts. Through a comprehensive reanalysis of the New Testament data, we argue that the optative is ‘pushed out’ of the strict domain of modality and syntax and into that of illocution and pragmatics. Evidence from ancient grammatical thought, sociolinguistics, and language contact corroborates this view

Emergency preparedness and management at the University of L’aquila (Central Italy) and the role of students’ associations in the April 6th 2009 earthquake

none3noOn April 6th 2009 an earthquake of Mw=6.3 hit the historical downtown of L’Aquila and its hinterland causing more than 300 fatalities and severe damage to private and public buildings. At the time, the University of L’Aquila represented a major source of employment and income for the city. The earthquake impacted both the facilities and the administrative, financial and patrimonial activities of the university, bringing into the open the tendency – widespread in Italy – to rely on adaptive tactics rather than on strategic pre-disaster plans. This paper investigates the university’s emergency preparedness and response capability and the strategies adopted to restore the education activities as well as avoid students migration to other universities. In addition, emphasis is placed on the role played by Student Associations in pre and post-disaster phases, and how students perceived the activities performed by these associations. Methods: To achieve this goal, it was undertaken: i) qualitative evaluation to assess the impact of earthquake on services and facilities of the university, the emergency preparedness and the measures adopted to face the emergency, ii) survey on the role played by Student Associations, both in emergency preparedness and response, according to students’ perception; iii) quantitative analysis to measure changes in the enrollment trend after the earthquake, and how university policies could curb students’ migration. Results: The policies adopted by the University allowed to diminish students’ migration; however, the measures taken by the university were based on an ad hoc plan as no emergency and continuity plans were prepared in advance. Similarly Student Associations got involved more in restoration activities than in emergency preparedness and risk awareness promotion. Discussion: Greater awareness and involvement are essential at each level (administrators, faculties, students) to plan in advance for an adverse scenario and to make important steps forward in understanding and embracing a culture of safety. The present paper is starting point for future research to deepen the emergency preparedness of Universities and the role that Student Associations may play to support and spread such a culture of safety.openMagni, Michele; Fraboni, Rita; Marincioni, FaustoMagni, Michele; Fraboni, Rita; Marincioni, Faust

Ionic events induced by epidermal growth factor. Evidence that hyperpolarization and stimulated cation influx play a role in the stimulation of cell growth.

Charybdotoxin, a blocker of K+ channels, and the imidazole drug SC38249, a blocker of both voltage- and second messenger-operated Ca2+ channels, were employed in mouse NIH-3T3 fibroblasts overexpressing the epidermal growth factor (EGF) receptor 1) to characterize the ionic events activated by EGF; and 2) to establish the role of those events in cell growth. The [Ca2+]i response by EGF was little changed by charybdotoxin while the parallel hyperpolarization was inhibited in a dose-dependent manner. At high toxin concentrations (greater than 3 x 10(-8) M), the effect of EGF on membrane potential was turned into a persistent depolarization sustained by both Na+ and Ca2+. Pretreatment with 10 microM SC38249 induced only minor changes of the intracellular Ca2+ release by EGF (the process responsible for the initial phase of the [Ca2+]i and membrane potential responses) and blocked the persistent, second phase [Ca2+]i and the hyperpolarization responses, both dependent on Ca2+ influx, as well as the depolarization in the charybdotoxin-pretreated cells. Long term (up to 2-day) treatment with either charybdotoxin or SC38249 failed to affect the viability and growth of unstimulated EGFR-T17 cells. Moreover, in these cells, the ionic responses to EGF were restored after a 30-min incubation in fresh medium. In contrast, growth stimulated by EGF was inhibited, moderately (-20%) by charybdotoxin and markedly (-60%) by SC38249. These results indicate for the first time that both hyperpolarization and, especially, the persistent increase of [Ca2+]i sustained by Ca2+ influx play a role in the activity of EGF, ultimately cooperating with other intracellular events in mitogenesis

The effect of epidermal growth factor on membrane potential. Rapid hyperpolarization followed by persistent fluctuations.

The effects of epidermal growth factor (EGF) on membrane potential were investigated in suspensions of the following three cell types endowed with a large complement of specific receptors: EGFR-T17 (a clone of mouse NIH-3T3 fibroblasts overexpressing EGF receptors); A431 and KB (two human carcinoma lines). In all these lines EGF induced a rapid and marked hyperpolarization constituted by an initial peak (in all three cell lines) and a subsequent sustained plateau phase, concomitant with the well-known increase of [Ca2+]i. The time course and phorbol ester inhibitability of the membrane potential effects were the same as for the [Ca2+]i response. Experiments with Na+-free and chloride-free media excluded a major role of the latter ions in the EGF-induced hyperpolarization. In contrast, experiments with high K+ media, with the monovalent cation ionophore gramicidin and with Ca2+-free media together with either a Ca2+ ionophore (ionomycin, in A431 and EGFR-T17), or an agonist (bradykinin, in A431) addressed to a receptor coupled to phosphoinositide hydrolysis, were consistent with the involvement of Ca2+-activated K+ channels. The EGF-induced hyperpolarization was completely blocked by the K+ channel blocker, quinidine, and unaffected by a variety of other drugs. Patch clamping of individual EGFR-T17 cells confirmed the initial hyperpolarization (from approximately -30 mV, the resting potential, to -60, -80 mV) was due to activation of an outward current. This initial hyperpolarization was followed by fluctuations (period approximately 1 min) persisting as long as the cells could be analyzed. Thus, the changes of membrane potential appear to be not only novel members of the group of early events triggered by EGF in target cells but also long-lasting effects of the growth factor, which continue for unexpectedly long periods of time after EGF application

Intracellular calcium regulates the tyrosine kinase receptor encoded by the MET oncogene.

Previous work (Gandino, L., Di Renzo, M. F., Giordano, S., Bussolino, F., and Comoglio, P.M. (1990) Oncogene 5, 721-725) has shown that the tyrosine kinase activity of the receptor encoded by the MET protooncogene is negatively modulated by protein kinase C (PKC). We now show that an increase of intracellular Ca2+ has a similar inhibitory effect in vivo, via a PKC-independent mechanism. In GTL-16 cells the p145MET kinase is overexpressed and constitutively phosphorylated on tyrosine. A rapid and reversible decrease of p145MET tyrosine phosphorylation was induced by treatment with the calcium ionophores A23187 or ionomycin. Experiments performed with the ionophores in absence of extracellular calcium showed that a rise in cytoplasmic Ca2+ concentration to 450 nM (due to release from intracellular stores) resulted in a similar effect. These Ca2+ concentrations had no effect on p145MET autophosphorylation in an in vitro kinase assay. This suggests that the effect of Ca2+ on p145MET tyrosine phosphorylation is not direct but may be mediated by Ca(2+)-activated proteins(s). Involvement of Ca(2+)-dependent tyrosine phosphatases was ruled out by experiments carried out in presence of Na2VO4. In vivo labeling with [32P]orthophosphate showed that the rise of intracellular Ca2+ induces serine phosphorylation of p145MET on a specific phosphopeptide. This suggests that Ca2+ negatively modulates p145MET kinase through the phosphorylation of a critical serine residue by a Ca(2+)-activated serine kinase distinct from PKC

Global streamflow modelling using process-informed machine learning

We present a novel hybrid framework that incorporates information from the process-based global hydrological model PCR-GLOBWB, to reduce prediction errors in streamflow simulations. In addition to catchment attributes and meteorological data, our methodology employs simulated streamflow and state variables from PCR-GLOBWB as predictors of observed river discharge. These outputs are used in a random forest, trained on a global database of streamflow measurements, to improve estimates of simulated river discharge across the globe. PCR-GLOBWB was run for the years 1979–2019 at 30 arcmin and its inputs and outputs were upscaled from daily to monthly time steps. A single random forest model was trained with these state variables, meteorological data and catchment attributes, as predictors of observed streamflow at 2,286 stations worldwide. Model performance was evaluated using Kling–Gupta efficiency (KGE). Results based on cross-validation show that the model is capable of discerning between a variety of hydroclimatic conditions and river flow dynamics, improving KGE of PCR-GLOBWB simulations at more than 80% of testing locations and increasing median KGE from −0.03 in uncalibrated runs to 0.51 after post-processing. Performance boosts are usually independent of the availability of streamflow data, making our method a potential candidate in addressing prediction in poorly gauged and ungauged basins

Differential coupling of dopaminergic D2 receptors expressed in different cell types. Stimulation of phosphatidylinositol 4,5-bisphosphate hydrolysis in LtK- fibroblasts, hyperpolarization, and cytosolic-free Ca2+ concentration decrease in GH4C1 cells.

Dopaminergic D2 receptors are widely regarded as typical inhibitory receptors, as they both inhibit adenylyl cyclase and decrease the cytosolic free Ca2+ concentration ([Ca2+]i) by activating K+ channels. A D2 receptor has recently been cloned (Bunzow, J. R., Van Tol, H. H. M., Grandy, D. K., Albert, P., Salon, J., Christie, M. D., Machida, C. A., Neve, K. A., and Civelli, O. (1988) Nature 336, 783-787) and expressed in two different cell lines, pituitary GH4C1 cells and Ltk- fibroblasts, where it has been shown to induce inhibition of adenylyl cyclase. We have investigated the additional effector systems coupled to this receptor. The responses observed in the two cells lines, which express similar levels of receptors (0.5-1 x 10(5)/cell), were surprisingly different. In GH4C1 cells D2 receptors failed to affect phosphoinositide hydrolysis and induced a decrease of [Ca2+]i. This latter effect appears to be mediated by hyperpolarization, most likely due to the activation of K+ channels. In striking contrast, in Ltk- fibroblasts the D2 receptor induced a rapid stimulation of inositol(1,4,5)-trisphosphate (+73% at 15 s) followed by the other inositol phosphates, and an immediate increase of [Ca2+]i due to both Ca2+ mobilization from internal stores and influx from the extracellular medium. In both GH4C1 and Ltk- cells, the D2 receptor response was mediated by G protein(s) sensitive to pertussis toxin. The increases of inositol trisphosphate and [Ca2+]i observed in Ltk- cells required dopamine concentrations only slightly higher than those inhibiting adenylyl cyclase (EG50 = 25, 29, and 11 nM, respectively) and were comparable in magnitude to the responses induced by the endogenous stimulatory receptor agonists, thrombin and ATP. The results demonstrate that in certain cells D2 receptors are efficiently coupled to the stimulation of phosphoinositide hydrolysis. The nature of receptor responses appears therefore to depend on the specific properties not only of the receptor molecule but also of the cell type in which it is expressed

Societal Controversies in Wikipedia Articles

Collaborative content creation inevitably reaches situations where different points of view lead to conflict. We focus on Wikipedia, the free encyclopedia anyone may edit, where disputes about content in controversial articles often reflect larger societal debates. While Wikipedia has a public edit history and discussion section for every article, the substance of these sections is difficult to phantom for Wikipedia users interested in the development of an article and in locating which topics were most controversial. In this paper we present Contropedia, a tool that augments Wikipedia articles and gives insight into the development of controversial topics. Contropedia uses an efficient language agnostic measure based on the edit history that focuses on wiki links to easily identify which topics within a Wikipedia article have been most controversial and when

Autonomous rotary mower versus ordinary reel Mower-effects of cutting height and nitrogen rate on manila grass turf quality

High-quality sports turfs often require low mowing and frequent maintenance. Sports turfs often consist of hard-to-mow warm season turfgrasses, such as zoysiagrass (Zoysia sp.) or bermudagrass (Cynodon sp.). Although autonomous mowers have several advantages over manually operated mowers, they are not designed to mow lower than 2.0 cm and are consequently not used on high-quality sports turfs. All autonomous mowers are only equipped with rotary mowing devices and do not perform clipping removal. An ordinary autonomous mower was modified to obtain a prototype autonomous mower cutting at a low height. The prototype autonomous mower was tested on a manila grass (Zoysia matrella) turf and compared its performance in terms of turf quality and energy consumption with an ordinary autonomous mower and with a gasoline reel mower. A three-way factor experimental design with three replications was adopted. Factor A consisted of four nitrogen rates (0, 50, 100, and 150 kghaL1), factor B consisted of two mowing systems (autonomous mower vs. walk-behind gasoline reel mower with no clipping removal), and factor C consisted of two mowing heights (1.2 and 3.6 cm). Prototype autonomous mower performed mowing at 1.2-cm mowing height whereas ordinary autonomous mower mowed at 3.6-cm mowing height. The interaction between the mowing system and mowing height showed that the turf quality was higher when the turf was mowed by the autonomous mower and at 1.2 cm than at 3.6 cm. Autonomous mowing not only reduced the mowing quality, but also reduced the leaf width. Lower mowing height induced thinner leaves. Nitrogen fertilization not only increased the overall turf quality, reduced weed cover percentage, but also reduced mowing quality. Autonomous mowers also had a lower energy consumption if compared with the reel mower (1.86 vs. 5.37 kWh/ week at 1.2-cm mowing height and 1.79 vs. 2.32 kWh/week at 3.6-cm mowing height, respectively). These results show that autonomous mowers can perform low mowing even on tough-to-mow turfgrass species. They could also be used on highquality sports turfs, thus saving time as well as reducing noise and pollution

Steaming and flaming for converting cool-season turfgrasses to hybrid bermudagrass in untilled soil

Turfgrass species can be classified into two main groups: cool-season and warm-season species. Warm-season species are more suited to a Mediterranean climate. Transplanting is a possible method to convert a cool-season to a warmseason turfgrass in untilled soil. It generally requires the chemical desiccation of the cool-season turfgrass. However, alternative physical methods, like flaming and steaming, are also available. This paper compares flaming, steaming, and herbicide application to desiccate cool-season turfgrass, for conversion to hybrid bermudagrass (Cynodon dactylon x C. transvaalensis) in untilled soil, using transplanting. Two prototype machines were used, a self-propelled steaming machine and a tractor-mounted liquefied petroleum gas flaming machine. Treatments compared in this work were two flaming treatments and two steaming treatments performed at four different doses together with two chemical treatments with glufosinateammonium herbicide applications. The cool-season turfgrass species were tall fescue (Festuca arundinacea) and perennial ryegrass (Lolium perenne). The desiccation effect of the various treatments on cool-season turf was assessed by photographic survey 15 days after treatment. The percentage cover of hybrid bermudagrass was visually assessed at 43 weeks after planting. Steaming and flaming effects on both parameters were described by logistic curves. The highest doses of steaming and flaming almost completely desiccated cool-season turf, and similar hybrid bermudagrass cover was established by both the methods as the chemical application (50% to 60%). Thus both flaming and steaming may be considered as valid alternatives to herbicides aimed at turf conversion