ETUDE DE PHASE I/II DE LA COMBINAISON DE TIRAPAZAMINE A L'ASSOCIATION CISPLATINE-VINORELBINE CHEZ LES PATIENTS ATTEINTS DE CARCINOME BRONCHIQUE NON A PETITES CELLULES AVANCE

PARIS12-CRETEIL BU Médecine (940282101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

ETUDE DE PHASE I/II DE LA COMBINAISON DE TIRAPAZAMINE A L'ASSOCIATION CISPLATINE-VINORELBINE CHEZ LES PATIENTS ATTEINTS DE CARCINOME BRONCHIQUE NON A PETITES CELLULES AVANCE

PARIS12-CRETEIL BU Médecine (940282101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Differential Responses of Vanilla Accessions to Root Rot and Colonization by [i]Fusarium oxysporum[/i] f. sp [i]radicis-vanillae[/i]

UMR AGAP - équipe PHIV - Plateforme d'histocytologie et d'imagerie cellulaire végétaleRoot and stem rot (RSR) disease caused by Fusariurn oxysporurn f. sp. radicis-vanillae (Forv) is the most damaging disease of vanilla (Vanilla planifolia and V. x tahitensis, Orchidaceae). Breeding programs aimed at developing resistant vanilla varieties are hampered by the scarcity of sources of resistance to RSR and insufficient knowledge about the histopathology of Forv. In this work we have (i) identified new genetic resources resistant to RSR including V planifolia inbreds and vanilla relatives, (ii) thoroughly described the colonization pattern of Forv into selected vanilla accessions, confirming its necrotic non-vascular behavior in roots, and (iii) evidenced the key role played by hypodermis, and particularly lignin deposition onto hypodermal cell walls, for resistance to Forv in two highly resistant vanilla accessions. Two hundred and fifty-four vanilla accessions were evaluated in the field under natural conditions of infection and in controlled conditions using in vitro plants root-dip inoculated by the highly pathogenic isolate Fo072. For the 26 accessions evaluated in both conditions, a high correlation was observed between field evaluation and in vitro assay. The root infection process and plant response of one susceptible and two resistant accessions challenged with Fo072 were studied using wide field and multiphoton microscopy. In susceptible V planifolia, hyphae penetrated directly into the rhizodermis in the hairy root region then invaded the cortex through the passage cells where it induced plasmolysis, but never reached the vascular region. In the case of the resistant accessions, the penetration was stopped at the hypodermal layer. Anatomical and histochemical observations coupled with spectral analysis of the hypodermis suggested the role of lignin deposition in the resistance to Forv. The thickness of lignin constitutively deposited onto outer cell walls of hypodermis was highly correlated with the level of resistance for 21 accessions tested. The accumulation of p-coumaric and sinapic acids, two phenolic precursors of lignin, was observed in the resistant plants inoculated with Fo072, but not in the susceptible one. Altogether, our analyses enlightened the mechanisms at work in RSR resistant genotypes and should enhance the development of novel breeding strategies aimed at improving the genetic control of RSR of vanilla

Homogenous static magnetic field coils dedicated to portable nuclear magnetic resonance for agronomic studies

International audienceNuclear magnetic resonance (NMR) experiments are usually carried out at very high and homogenous magnetic fields. However, portable systems have emerged in recent years at low fields, offering many advantages like easier maintenance and open design that permit us to imagine a variety of coil configurations in order to maximize the homogeneity of the static field B-0 and the radio-frequency field B-1. The wire winding distribution based on space harmonics suppression (SHS) of the cosine current density distribution offers new possibilities of producing homogenous static fields B-0. In this work, an extension of the SHS method, allowing homogeneity enhancement and flexibility of the coil design, is presented. The efficiency of the method is demonstrated through the construction of homogenous magnetic field coils dedicated to a very low magnetic field (8 mT) H-1 NMR spectrometer. The performance of these coils in terms of magnetic field intensity and homogeneity as well as self-heating behavior is compliant with in planta experiments for agronomic studies

BOBINES HOMOGÈNES POUR LA RÉSONANCE MAGNÉTIQUE NUCLÉAIRE PORTABLE (RMN) APPLIQUÉE À L'AGROÉCOLOGIE

International audienc

Who Were Hospitalized Deceased Patients from COVID-19 During the First Year of Pandemic? Retrospective Analysis of 1104 Deceased Patients in South of France

International audienceAbstract Introduction Following the first year of the COVID-19 pandemic, a complete analysis of the characteristics of the deceased hospitalized patients was performed, to identify factors related to premature mortality and to compare patient profiles according to the epidemic periods. Methods Retrospective analysis of 1104 deceased patients in two University Hospitals in South-eastern France, between March 1, 2020 and March 12, 2021 from Hospital’s electronic medical records was performed. Results Mean age was 80 years (± 11.1) and 10% of the deceased were younger than 65 years with specific comorbidities, e.g., genetic conditions, metastatic cancer, or massive obesity. Among the three clusters identified, two clusters (75% of deceased patients) include very elderly patients with numerous comorbidities, and differ by their proportion of dependent institutionalized patients. The third cluster is made up of younger patients with fewer but severe comorbidities. Deceased patients’ profiles varied according to the epidemic periods: during the first period (March–June 2020), more patients were institutionalized. The second period (September–December2020) coincided with a higher mortality rate. Conclusions This study confirmed that most patients hospitalized and dying from COVID-19 were frail, i.e., elderly and/or highly comorbid and that the small proportion of young patients had severe comorbidities