Integrated Proteomic and Metabolomic Profiling of Phytophthora cinnamomi Attack on Sweet Chestnut (Castanea sativa) Reveals Distinct Molecular Reprogramming Proximal to the Infection Site and Away from It

Phytophthora cinnamomi is one of the most invasive tree pathogens that devastates wild and cultivated forests. Due to its wide host range, knowledge of the infection process at the molecular level is lacking for most of its tree hosts. To expand the repertoire of studied Phytophthora-woody plant interactions and identify molecular mechanisms that can facilitate discovery of novel ways to control its spread and damaging effects, we focused on the interaction between P. cinnamomi and sweet chestnut (Castanea sativa), an economically important tree for the wood processing industry. By using a combination of proteomics, metabolomics, and targeted hormonal analysis, we mapped the effects of P. cinnamomi attack on stem tissues immediately bordering the infection site and away from it. P. cinnamomi led to a massive reprogramming of the chestnut proteome and accumulation of the stress-related hormones salicylic acid (SA) and jasmonic acid (JA), indicating that stem inoculation can be used as an easily accessible model system to identify novel molecular players in P. cinnamomi pathogenicity.O

A survey in natural forest ecosystems of Vietnam reveals high diversity of both new and described Phytophthora taxa including P. ramorum

In 2016 and 2017, surveys of Phytophthora diversity were performed in 25 natural and semi-natural forest stands and 16 rivers in temperate and subtropical montane and tropical lowland regions of Vietnam. Using baiting assays from soil samples and rivers and direct isolations from naturally fallen leaves, 13 described species, five informally designated taxa and 21 previously unknown taxa of Phytophthora were isolated from 58 of the 91 soil samples (63.7%) taken from the rhizosphere of 52 of the 64 woody plant species sampled (81.3%) in 20 forest stands (83.7%), and from all rivers: P. capensis, P. citricola VII, VIII, IX, X and XI, P. sp. botryosa-like 2, P. sp. meadii-like 1 and 2, P. sp. tropicalis-like 2 and P. sp. multivesiculata-like 1 from Phytophthora major phylogenetic Clade 2; P. castaneae and P. heveae from Clade 5; P. chlamydospora, P. gregata, P. sp. bitahaiensis-like and P. sp. sylvatica-like 1, 2 and 3 from Clade 6; P. cinnamomi (Pc), P. parvispora, P. attenuata, P. sp. attenuata-like 1, 2 and 3 and P. ×heterohybrida from Clade 7; P. drechsleri, P. pseudocryptogea, P. ramorum (Pr) and P. sp. kelmania from Clade 8, P. macrochlamydospora, P. sp. ×insolita-like, P. sp. ×kunnunara-like, P. sp. ×virginiana-like s.l. and three new taxa, P. sp. quininea-like, P. sp. ×Grenada 3-like and P. sp. ×Peru 4-like, from Clade 9; and P. sp. gallica-like 1 and 2 from Clade 10. The A1 and A2 mating types of both Pc and Pr co-occurred. The A2 mating type of Pc was associated with severe dieback of montane forests in northern Vietnam. Most other Phytophthora species, including Pr, were not associated with obvious disease symptoms. It is concluded that (1) Vietnam is within the center of origin of most Phytophthora taxa found including Pc and Pr, and (2) Phytophthora clades 2, 5, 6, 7, 8, 9, and 10 are native to Indochina.info:eu-repo/semantics/publishedVersio

Ligninolytické enzymy rodu Trametes

Eighty-four strains belonging to four species of the genus Trametes Fr. (T. versicolor, T. hirsuta, T. ochracea and T. cervina) collected in different localities and on different substrates were screened for laccase production and eight of them with their monokaryotic derivates were then examined in more detail for their ability to produce also other lignin-modifying enzymes (manganese peroxidase and lignin peroxidase). Production variability was relatively high and no significant correlation was found between the origin of the strains (locality, substrate) and the enzyme production. Dikaryons of all three species (but not of all their strains) exhibited LiP activity, which was not detected in the respective monokaryons

Morphological and phylogenetic studies of two new neotropical species of Loweomyces (Polyporales, Basidiomycota)

The genus Loweomyces is characterized by resupinate to pileate, sessile to stipitate basidiomes, monomitic to dimitic hyphal system with presence of pseudoskeletal hyphae, thin-walled hymenial cystidia, and usually cyanophilous spore and hyphal walls. The genus has not been widely accepted and some authors placed Loweomyces species in Abortiporus, Tyromyces or Antrodiella. During a survey of polypores in southern Brazil, two unidentified species with similar features to Loweomyces spp. were found. Morphological and molecular studies were carried out in order to understand their morphological and evolutionary relationships and to confirm their phylogenetic placement and taxonomic identity. DNA sequences of the internal transcribed spacer and large subunit of ribosomal RNA gene were used for phylogenetic studies. Both studied species have similar morphological characteristics to other Loweomyces spp. and group close to L. fractipes phylogenetically; therefore, Loweomyces spissus and L. tomentosus are proposed as new species. Full descriptions, illustrations of microscopic features, and an identification key to the accepted species in the genus are presented.Fil: Westphalen, Mauro C.. Instituto de Botanica de Sao Paulo; BrasilFil: Tomšovský, Michal. Mendelova Univerzita V Brne; República ChecaFil: Rajchenberg, Mario. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gugliotta, Adriana M.. Instituto de Botanica de Sao Paulo; Brasi

The effect of military boots on front kick dynamics

Front kicks are often used in combat activities that involve the use of kicks and punches, including close combat military training. To mimic real-life combative environments where soldiers execute forceful front kicks while wearing military boots, it is logical to wear these boots during close combat training. However, the effect of military footwear on front kick forces remain unknown. Therefore, the aim of this study was to compare the effects of military boots on front kick dynamics. Six male soldiers (23.3 ± 1.7 yr, 74.3 ± 6.2 kg, 175.5 ± 4.5 cm) performed eight individual front kicks with no military boots, in bare feet (NB) and with military boots (MB). Peak force (N), impulse (N.s) and time to reach peak force (ms), were measured during each kick. Data were analyzed using paired sample t-tests or nonparametric Wilcoxon pair test and Cohen’s d. Results. Neither peak force (3180 ± 647 N) nor impulse (367 ± 40 N.s) of MB were different than NB (3157 ± 291 N and 360 ± 48 N.s, respectively) (p = 0.85; d = 0.04 and p = 0.36; d = 0.12, respectively). Peak force was reached in a significantly shorter time during MB (39 ± 16 ms) compared to NB (56 ± 8 ms; d = −0.88). Conclusion. Peak force was reached in a shorter time using MB compared to barefoot (NB) front kicks. The use of military boots changes the time course of peak force during the front kick

Worldwide genetic structure elucidates the Eurasian origin and invasion pathways of Dothistroma septosporum, causal agent of Dothistroma needle blight

Dothistroma septosporum, the primary causal agent of Dothistroma needle blight, is one of the most significant foliar pathogens of pine worldwide. Its wide host and environmental ranges have led to its global success as a pathogen and severe economic damage to pine forests in many regions. This comprehensive global population study elucidated the historical migration pathways of the pathogen to reveal the Eurasian origin of the fungus. When over 3800 isolates were examined, three major population clusters were revealed: North America, Western Europe, and Eastern Europe, with distinct subclusters in the highly diverse Eastern European cluster. Modeling of historical scenarios using approximate Bayesian computation revealed the North American cluster was derived from an ancestral population in Eurasia. The Northeastern European subcluster was shown to be ancestral to all other European clusters and subclusters. The Turkish subcluster diverged first, followed by the Central European subcluster, then the Western European cluster, which has subsequently spread to much of the Southern Hemisphere. All clusters and subclusters contained both mating-types of the fungus, indicating the potential for sexual reproduction, although asexual reproduction remained the primary mode of reproduction. The study strongly suggests the native range of D. septosporum to be in Eastern Europe (i.e., the Baltic and Western Russia) and Western Asia