Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits.

Spontaneous and sensory-evoked activity propagates across varying spatial scales in the mammalian cortex, but technical challenges have limited conceptual links between the function of local neuronal circuits and brain-wide network dynamics. We present a method for simultaneous cellular-resolution two-photon calcium imaging of a local microcircuit and mesoscopic widefield calcium imaging of the entire cortical mantle in awake mice. Our multi-scale approach involves a microscope with an orthogonal axis design where the mesoscopic objective is oriented above the brain and the two-photon objective is oriented horizontally, with imaging performed through a microprism. We also introduce a viral transduction method for robust and widespread gene delivery in the mouse brain. These approaches allow us to identify the behavioral state-dependent functional connectivity of pyramidal neurons and vasoactive intestinal peptide-expressing interneurons with long-range cortical networks. Our imaging system provides a powerful strategy for investigating cortical architecture across a wide range of spatial scales

Chandra X-ray and Hubble Space Telescope Imaging of Optically Selected kiloparsec-Scale Binary Active Galactic Nuclei I. Nature of the Nuclear Ionizing Sources

Kiloparsec-scale binary active galactic nuclei (AGNs) signal active supermassive black hole (SMBH) pairs in merging galaxies. Despite their significance, unambiguously confirmed cases remain scarce and most have been discovered serendipitously. In a previous systematic search, we optically identified four kpc-scale binary AGNs from candidates selected with double-peaked narrow emission lines at redshifts of 0.1--0.2. Here we present Chandra and Hubble Space Telescope Wide Field Camera 3 (WFC3) imaging of these four systems. We critically examine and confirm the binary-AGN scenario for two of the four targets, by combining high angular resolution X-ray imaging spectroscopy with Chandra ACIS-S, better nuclear position constraints from WFC3 F105W imaging, and direct starburst estimates from WFC3 F336W imaging; for the other two targets, the existing data are still consistent with the binary-AGN scenario, but we cannot rule out the possibility of only one AGN ionizing gas in both merging galaxies. We find tentative evidence for a systematically smaller X-ray-to-[O III] luminosity ratio and/or higher Compton-thick fraction in optically selected kpc-scale binary AGNs than in single AGNs, possibly caused by a higher nuclear gas column due to mergers and/or a viewing angle bias related to the double-peak narrow line selection. While our result lends some further support to the general approach of optically identifying kpc-scale binary AGNs, it also highlights the challenge and ambiguity of X-ray confirmation.Comment: 18 emulateapj pages, 5 figures, ApJ in pres

The size distribution of galaxies in the Sloan Digital Sky Survey

abridged: We use a complete sample of about 140,000 galaxies from the Sloan Digital Sky Survey (SDSS) to study the size distribution of galaxies and its dependence on their luminosity, stellar mass, and morphological type. The large SDSS database provides statistics of unprecedented accuracy. For each type of galaxy, the size distribution at given luminosity (or stellar mass) is well described by a log-normal function, characterized by its median $\bar{R}$ and dispersion $\sigma_{\ln R}$. For late-type galaxies, there is a characteristic luminosity at $M_{r,0}\sim -20.5$ (assuming $h=0.7$) corresponding to a stellar mass M_0\sim 10^{10.6}\Msun. Galaxies more massive than $M_0$ have $\bar{R}\propto M^{0.4}$ and $\sigma_{\ln R}\sim 0.3$, while less massive galaxies have $\bar{R}\propto M^{0.15}$ and $\sigma_{\ln R}\sim 0.5$. For early-type galaxies, the $\bar{R}$ - $M$ relation is significantly steeper, $\bar{R}\propto M^{0.55}$, but the $\sigma_{\ln R}$ - $M$ relation is similar to that of late-type galaxies. Faint red galaxies have sizes quite independent of their luminosities.Comment: 42 pages, 18 figures, 2 tables; replaced with the version accepted by MNRA

Effects of Signaling on Learner Engagement in Informal Learning on YouTube

Millions of educational videos available on YouTube offer unprecedented learning opportunities. A considerable number of studies have been directed toward YouTube educational videos. Yet, research on learner engagement with YouTube educational videos is scarce, despite its central role in learning. This paper addresses this research gap. We adopt the conceptualization that learner engagement has three dimensions - behavioral, emotional, and cognitive- and investigate how signaling in YouTube educational video presentation affects learner engagement in informal learning. Our analysis shows that signaling positively affects behavioral, emotional, and cognitive engagement. These findings substantiate the empirical knowledge on learner engagement with YouTube educational videos. Our study corroborates traditional video engagement research and extends its relevance to the social media learning environment. It also informs video designers and developers on adding features in the video presentation to optimize learner engagement with YouTube educational videos

Human P450 CYP17A1: Control of Substrate Preference by Asparagine 202

CYP17A1 is a key steroidogenic enzyme known to conduct several distinct chemical transformations on multiple substrates. In its hydroxylase activity, this enzyme adds a hydroxyl group at the 17α position of both pregnenolone and progesterone at approximately equal rates. However, the subsequent 17,20 carbon–carbon scission reaction displays variable substrate specificity in the numerous CYP17A1 isozymes operating in vertebrates, manifesting as different Kd and kcat values when presented with 17α-hydroxypregnenlone (OHPREG) versus 17α-hydroxyprogesterone (OHPROG). Here we show that the identity of the residue at position 202 in human CYP17A1, thought to form a hydrogen bond with the A-ring alcohol substituent on the pregnene- nucleus, is a key driver of this enzyme’s native preference for OHPREG. Replacement of asparagine 202 with serine completely reverses the preference of CYP17A1, more than doubling the rate of turnover of the OHPROG to androstenedione reaction and substantially decreasing the rate of formation of dehydroepiandrosterone from OHPREG. In a series of resonance Raman experiments, it was observed that, in contrast with the case for the wild-type protein, in the mutant the 17α alcohol of OHPROG tends to form a H-bond with the proximal rather than terminal oxygen of the oxy–ferrous complex. When OHPREG was a substrate, the mutant enzyme was found to have a H-bonding interaction with the proximal oxygen that is substantially weaker than that of the wild type. These results demonstrate that a single-point mutation in the active site pocket of CYP17A1, even when far from the heme, has profound effects on steroidogenic selectivity in androgen biosynthesis

Ultra-thin Topological Insulator Bi2Se3 Nanoribbons Exfoliated by Atomic Force Microscopy

Ultra-thin topological insulator nanostructures, in which coupling between top and bottom surface states takes place, are of great intellectual and practical importance. Due to the weak Van der Waals interaction between adjacent quintuple layers (QLs), the layered bismuth selenide (Bi2Se3), a single Dirac-cone topological insulator with a large bulk gap, can be exfoliated down to a few QLs. In this paper, we report the first controlled mechanical exfoliation of Bi2Se3 nanoribbons (> 50 QLs) by an atomic force microscope (AFM) tip down to a single QL. Microwave impedance microscopy is employed to map out the local conductivity of such ultra-thin nanoribbons, showing drastic difference in sheet resistance between 1~2 QLs and 4~5 QLs. Transport measurement carried out on an exfoliated (\leq 5 QLs) Bi2Se3 device shows non-metallic temperature dependence of resistance, in sharp contrast to the metallic behavior seen in thick (> 50 QLs) ribbons. These AFM-exfoliated thin nanoribbons afford interesting candidates for studying the transition from quantum spin Hall surface to edge states

Impact of Natural Blind Spot Location on Perimetry.

We study the spatial distribution of natural blind spot location (NBSL) and its impact on perimetry. Pattern deviation (PD) values of 11,449 reliable visual fields (VFs) that are defined as clinically unaffected based on summary indices were extracted from 11,449 glaucoma patients. We modeled NBSL distribution using a two-dimensional non-linear regression approach and correlated NBSL with spherical equivalent (SE). Additionally, we compared PD values of groups with longer and shorter distances than median, and larger and smaller angles than median between NBSL and fixation. Mean and standard deviation of horizontal and vertical NBSL were 14.33° ± 1.37° and -2.06° ± 1.27°, respectively. SE decreased with increasing NBSL (correlation: r = -0.14, p \u3c 0.001). For NBSL distances longer than median distance (14.32°), average PD values decreased in the upper central (average difference for significant points (ADSP): -0.18 dB) and increased in the lower nasal VF region (ADSP: 0.14 dB). For angles in the direction of upper hemifield relative to the median angle (-8.13°), PD values decreased in lower nasal (ADSP: -0.11 dB) and increased in upper temporal VF areas (ADSP: 0.19 dB). In conclusion, we demonstrate that NBSL has a systematic effect on the spatial distribution of VF sensitivity

TrackMapper Rises

This project repaired and upgraded non-functional Department of Agriculture and Fisheries (DAF) TrackMapper software, to a faster, functional, user-friendly, web-based application that can be directly accessed by researchers, fishery managers and others. TrackMapper is database software that was developed by DAF researchers in 2007 as part of an externally funded Fisheries Research and Development Corporation project (FRDC project 2002/056 Innovative stock assessment and effort mapping using VMS and electronic logbooks). However, over the last 5-6 years, the program has become incompatible with contemporary Windows-based operating platforms, rendering it inoperable. TrackMapper was developed for the Queensland east coast otter trawl fishery, which is the state’s most valuable commercial fishery, harvesting 7000-8000 t of seafood annually valued at $80-90 million. The most useful feature of TrackMapper is that it can produce maps of fishing effort, catch and catch rates for the fishery at a spatial resolution that is 10-50 times that reported using logbook data alone. This information can be used for a range of fisheries management and research tasks, including the assessment of targeted stocks of prawns, scallops, bugs and stout whiting, as well as impacts on the bottom and other non-target bycatch species. This is noteworthy as much of the fishery occurs in waters of the Great Barrier Reef Marine Park (GBRMP), which has World Heritage status