Lean Six Sigma and Assurance of Learning: Challenges and Opportunities

The importance, and associated challenges, of faculty ownership and engagement in the assurance of learning (AoL) process are well documented in the literature. The authors demonstrate how schools can further their AoL objectives by encouraging and subsequently supporting organic, faculty-led initiatives. Specifically, we present a case study of a faculty led initiative to explore and demonstrate, as a proof of concept, how Lean Six Sigma can be used to improve AoL practices

Testing of Noodle Creek Site 41JS102 Jones County, Texas

Staff Archeologists from the Environmental Affairs Division of Texas Department of Transportation discovered buried cultural resources that constitute archeological site 41JS102, in June and July 2001 at the proposed location of a bridge replacement over Noodle Creek in Jones County, Texas. Shovel testing and backhoe trenching revealed cultural material at 65 cm below the surface. Following this discovery the Texas Department of Transportation made recommendations to the Texas Historical Commission to assess site 41JS 102 and they concurred. In September 2001 archeologists from the Texas Department of Transportation initiated the site assessment phase. Landowner complications halted that work before completion. Subsequently, TRC Environmental was provided a Work Authorization under an existing general services contract to complete the assessment of 41JS102 in December 2001. The fieldwork consisted of the hand excavation and documentation of eight 1 by 1 m test units and the digging and documenting of six backhoe trenches. Limited and scattered historic artifacts were recovered from the upper parts of the two alluvial terraces (T0 and T1). A prehistoric component with quantities of lithic debitage and sparse burned rocks, limited stone tools and mussel shells, and one possible cultural feature were encountered in a sloping, but well defined 10 to 15 cm thick zone in the T1 deposits. The Principal Investigator recommended prehistoric site 4 1JS102 was eligible for inclusion in the National Register of Historic Places and as a State Archeological Landmark. However, the staff at Texas Department of Transportation disagreed with that recommendation and presented an opposing case to the Texas Historical Commission who concurred with the Texas Department of Transportation\u27s recommendations. Consequently, no further archeological investigations will occur prior to the proposed development

Whole genome sequencing of experimental hybrids supports meiosis-like sexual recombination in Leishmania

Hybrid genotypes have been repeatedly described among natural isolates of Leishmania, and the recovery of experimental hybrids from sand flies co-infected with different strains or species of Leishmania has formally demonstrated that members of the genus possess the machinery for genetic exchange. As neither gamete stages nor cell fusion events have been directly observed during parasite development in the vector, we have relied on a classical genetic analysis to determine if Leishmania has a true sexual cycle. Here, we used whole genome sequencing to follow the chromosomal inheritance patterns of experimental hybrids generated within and between different strains of L. major and L. infantum. We also generated and sequenced the first experimental hybrids in L. tropica. We found that in each case the parental somy and allele contributions matched the inheritance patterns expected under meiosis 97–99% of the time. The hybrids were equivalent to F1 progeny, heterozygous throughout most of the genome for the markers that were homozygous and different between the parents. Rare, non-Mendelian patterns of chromosomal inheritance were observed, including a gain or loss of somy, and loss of heterozygosity, that likely arose during meiosis or during mitotic divisions of the progeny clones in the fly or culture. While the interspecies hybrids appeared to be sterile, the intraspecies hybrids were able to produce backcross and outcross progeny. Analysis of 5 backcross and outcross progeny clones generated from an L. major F1 hybrid, as well as 17 progeny clones generated from backcrosses involving a natural hybrid of L. tropica, revealed genome wide patterns of recombination, demonstrating that classical crossing over occurs at meiosis, and allowed us to construct the first physical and genetic maps in Leishmania. Altogether, the findings provide strong evidence for meiosis-like sexual recombination in Leishmania, presenting clear opportunities for forward genetic analysis and positional cloning of important genes.</div

An integrated biochemical prediction model of all-cause mortality in patients undergoing lower extremity bypass surgery for advanced peripheral artery disease

BackgroundPatients with advanced peripheral artery disease (PAD) have a high prevalence of cardiovascular (CV) risk factors and shortened life expectancy. However, CV risk factors poorly predict midterm (<5 years) mortality in this population. This study tested the hypothesis that baseline biochemical parameters would add clinically meaningful predictive information in patients undergoing lower extremity bypass operations.MethodsThis was a prospective cohort study of patients with clinically advanced PAD undergoing lower extremity bypass surgery. The Cox proportional hazard model was used to assess the main outcome of all-cause mortality. A clinical model was constructed with known CV risk factors, and the incremental value of the addition of clinical chemistry, lipid assessment, and a panel of 11 inflammatory parameters was investigated using the C statistic, the integrated discrimination improvement index, and Akaike information criterion.ResultsThe study monitored 225 patients for a median of 893 days (interquartile range, 539-1315 days). In this study, 50 patients (22.22%) died during the follow-up period. By life-table analysis (expressed as percent surviving ± standard error), survival at 1, 2, 3, 4, and 5 years, respectively, was 90.5% ± 1.9%, 83.4% ± 2.5%, 77.5% ± 3.1%, 71.0% ± 3.8%, and 65.3% ± 6.5%. Compared with survivors, decedents were older, diabetic, had extant coronary artery disease, and were more likely to present with critical limb ischemia as their indication for bypass surgery (P < .05). After adjustment for the above, clinical chemistry and inflammatory parameters significant (hazard ratio [95% confidence interval]) for all-cause mortality were albumin (0.43 [0.26-0.71]; P = .001), estimated glomerular filtration rate (0.98 [0.97-0.99]; P = .023), high-sensitivity C-reactive protein (hsCRP; 3.21 [1.21-8.55]; P = .019), and soluble vascular cell adhesion molecule (1.74 [1.04-2.91]; P = .034). Of the inflammatory molecules investigated, hsCRP proved most robust and representative of the integrated inflammatory response. Albumin, eGFR, and hsCRP improved the C statistic and integrated discrimination improvement index beyond that of the clinical model and produced a final C statistic of 0.82.ConclusionsA risk prediction model including traditional risk factors and parameters of inflammation, renal function, and nutrition had excellent discriminatory ability in predicting all-cause mortality in patients with clinically advanced PAD undergoing bypass surgery

Southern Ocean GLOBEC moored array and automated weather station data report

Southern Ocean GLOBal ocean ECosystemsAs part of the U.S. Southern Ocean GLOBEC program, moored time series measurements of temperature, conductivity (salinity), pressure, velocity, and acoustic backscatter were made from March 2001 to March 2003 in and near Marguerite Bay, located on the Antarctic Peninsula western shelf. To monitor surface forcing during the moored array observations, two automatic weather stations (AWSs) were deployed on islands in Marguerite Bay and time series of wind, air temperature, pressure, and relative humidity were collected from May 2001 through March 2003. This report describes the individual moorings, their locations and local bathymetry, the instrumentation used and measurement depths, calibration and data processing steps taken to produce final time series, and basic plots of the final time series. The AWS data acquisition and processing are also described and basic plots of the final meteorological time series presented. Directions are given about how to access the raw and processed moored and AWS data via the SO GLOBEC website (http://globec.whoi.edu/jg/dir/globec/soglobec/).Funding was provided by the National Science Foundation under contract number OPP-99-10092