Imaging calcium microdomains within entire astrocyte territories and endfeet with GCaMPs expressed using adeno-associated viruses.

Intracellular Ca(2+) transients are considered a primary signal by which astrocytes interact with neurons and blood vessels. With existing commonly used methods, Ca(2+) has been studied only within astrocyte somata and thick branches, leaving the distal fine branchlets and endfeet that are most proximate to neuronal synapses and blood vessels largely unexplored. Here, using cytosolic and membrane-tethered forms of genetically encoded Ca(2+) indicators (GECIs; cyto-GCaMP3 and Lck-GCaMP3), we report well-characterized approaches that overcome these limitations. We used in vivo microinjections of adeno-associated viruses to express GECIs in astrocytes and studied Ca(2+) signals in acute hippocampal slices in vitro from adult mice (aged ∼P80) two weeks after infection. Our data reveal a sparkling panorama of unexpectedly numerous, frequent, equivalently scaled, and highly localized Ca(2+) microdomains within entire astrocyte territories in situ within acute hippocampal slices, consistent with the distribution of perisynaptic branchlets described using electron microscopy. Signals from endfeet were revealed with particular clarity. The tools and experimental approaches we describe in detail allow for the systematic study of Ca(2+) signals within entire astrocytes, including within fine perisynaptic branchlets and vessel-associated endfeet, permitting rigorous evaluation of how astrocytes contribute to brain function

Model-based design for restoration of a small urban river

A model-based design is presented for restoring the small urban river Panke located in Berlin, Germany. This new design process combines high resolution 2D hydraulic modeling with habitat modeling and river-ecological expert knowledge in a highly iterative way. Advances have been made for the habitat modeling: habitat suitability maps have been developed for fish and the habitat suitability for benthos has been assessed by including groups with different hydraulic preferences. Using the model-based design we have developed preference variants for the Panke which include structures such as pools, riffles, river banks, dead wood as well as aquatic vegetation. To account for the very detailed geometry of some structures such as dead wood, high resolution grids with edge length up to one decimeter have been generated. Furthermore flood protection has been assured. The variants should be constructed in the Panke in 2015. We expect that the ecological conditions for fish and benthos will improve, however this has to be evaluated by further measurements. The model-based approach for the design of enhancement measures delivered valuable hints on current shortcomings in the river morphology, priorities for the creation of new habitats and quantitative information on the increase of suitable areas to be expected. In addition, relating the habitat changes to different flow rates helped to estimate the temporal availability of high quality habitats after the implementation of the measures

QuantiFERON®-TB gold in-tube performance for diagnosing active tuberculosis in children and adults in a high burden setting.

To determine whether QuantiFERON®-TB Gold In-Tube (QFT) can contribute to the diagnosis of active tuberculosis (TB) in children in a high-burden setting and to assess the performance of QFT and tuberculin skin test (TST) in a prospective cohort of TB suspect children compared to adults with confirmed TB in Tanzania. Sensitivity and specificity of QFT and TST for diagnosing active TB as well as indeterminate QFT rates and IFN-γ levels were assessed in 211 TB suspect children in a Tanzanian district hospital and contrasted in 90 adults with confirmed pulmonary TB. Sensitivity of QFT and TST in children with confirmed TB was 19% (5/27) and 6% (2/31) respectively. In adults sensitivity of QFT and TST was 84% (73/87) and 85% (63/74). The QFT indeterminate rate in children and adults was 27% and 3%. Median levels of IFN-γ were lower in children than adults, particularly children <2 years and HIV infected. An indeterminate result was associated with age <2 years but not malnutrition or HIV status. Overall childhood mortality was 19% and associated with an indeterminate QFT result at baseline. QFT and TST showed poor performance and a surprisingly low sensitivity in children. In contrast the performance in Tanzanian adults was good and comparable to performance in high-income countries. Indeterminate results in children were associated with young age and increased mortality. Neither test can be recommended for diagnosing active TB in children with immature or impaired immunity in a high-burden setting

ETSI reconfigurable radio systems: status and future directions on software defined radio and cognitive radio standards

This article details the current work status of the ETSI Reconfigurable Radio Systems Technical Committee, positions the ETSI work with respect to other standards efforts (IEEE 802, IEEE SCC41) as well as the European Regulatory Framework, and gives an outlook on the future evolution. In particular, software defined radio related study results are presented with a focus on SDR architectures for mobile devices such as mobile phones. For MDs, a novel architecture and inherent interfaces are presented enabling the usage of SDR principles in a mass market context. Cognitive radio principles within ETSI RRS are concentrated on two topics, a cognitive pilot channel proposal and a Functional Architecture for Management and control of reconfigurable radio systems, including dynamic self-organizing planning and management, dynamic spectrum management, joint radio resource management. Finally, study results are indicated that are targeting a SDR/CR security framework.Postprint (published version

Can Microsoft Academic be used for citation analysis of preprint archives? The case of the Social Science Research Network

This is an accepted manuscript of an article published by Springer in Scientometrics on 07/03/2018, available online: https://doi.org/10.1007/s11192-018-2704-z The accepted version of the publication may differ from the final published version.Preprint archives play an important scholarly communication role within some fields. The impact of archives and individual preprints are difficult to analyse because online repositories are not indexed by the Web of Science or Scopus. In response, this article assesses whether the new Microsoft Academic can be used for citation analysis of preprint archives, focusing on the Social Science Research Network (SSRN). Although Microsoft Academic seems to index SSRN comprehensively, it groups a small fraction of SSRN papers into an easily retrievable set that has variations in character over time, making any field normalisation or citation comparisons untrustworthy. A brief parallel analysis of arXiv suggests that similar results would occur for other online repositories. Systematic analyses of preprint archives are nevertheless possible with Microsoft Academic when complete lists of archive publications are available from other sources because of its promising coverage and citation results

ETSI Reconfigurable Radio Systems – Status and Future Directions on Software Defined Radio and Cognitive Radio Standards

This article details the current work status of the ETSI Reconfigurable Radio Systems Technical Committee, positions the ETSI work with respect to other standards efforts (IEEE 802, IEEE SCC41) as well as the European Regulatory Framework, and gives an outlook on the future evolution. In particular, software defined radio related study results are presented with a focus on SDR architectures for mobile devices such as mobile phones. For MDs, a novel architecture and inherent interfaces are presented enabling the usage of SDR principles in a mass market context. Cognitive radio principles within ETSI RRS are concentrated on two topics, a cognitive pilot channel proposal and a Functional Architecture for Management and control of reconfigurable radio systems, including dynamic self-organizing planning and management, dynamic spectrum management, joint radio resource management. Finally, study results are indicated that are targeting a SDR/CR security framework