Steady-state crystallization of Rydberg excitations in an optically driven lattice gas

We study resonant optical excitations of atoms in a one-dimensional lattice to the Rydberg states interacting via the van der Waals potential which suppresses simultaneous excitation of neighboring atoms. Considering two- and three-level excitation schemes, we analyze the dynamics and stationary state of the continuously-driven, dissipative many-body system employing time-dependent density-matrix renormalization group (t-DMRG) simulations. We show that two-level atoms can exhibit only nearest neighbor correlations, while three-level atoms under dark-state resonant driving can develop finite-range crystalline order of Rydberg excitations. We present an approximate rate equation model whose analytic solution yields qualitative understanding of the numerical results.Comment: 5 pages,3 figure

Cre-dependent DNA recombination activates a STING-dependent innate immune response

Gene-recombinase technologies, such as Cre/loxP-mediated DNA recombination, are important tools in the study of gene function, but have potential side effects due to damaging activity on DNA. Here we show that DNA recombination by Cre instigates a robust antiviral response in mammalian cells, independent of legitimate loxP recombination. This is due to the recruitment of the cytosolic DNA sensor STING, concurrent with Cre-dependent DNA damage and the accumulation of cytoplasmic DNA. Importantly, we establish a direct interplay between this antiviral response and cell-cell interactions, indicating that low cell densities in vitro could be useful to help mitigate these effects of Cre. Taking into account the wide range of interferon stimulated genes that may be induced by the STING pathway, these results have broad implications in fields such as immunology, cancer biology, metabolism and stem cell research. Further, this study sets a precedent in the field of gene-engineering, possibly applicable to other enzymatic-based genome editing technologies

Cre-dependent DNA recombination activates a STING-dependent innate immune response

Gene-recombinase technologies, such as Cre/loxP-mediated DNA recombination, are important tools in the study of gene function, but have potential side effects due to damaging activity on DNA. Here we show that DNA recombination by Cre instigates a robust antiviral response in mammalian cells, independent of legitimate loxP recombination. This is due to the recruitment of the cytosolic DNA sensor STING, concurrent with Cre-dependent DNA damage and the accumulation of cytoplasmic DNA. Importantly, we establish a direct interplay between this antiviral response and cell-cell interactions, indicating that low cell densities in vitro could be useful to help mitigate these effects of Cre. Taking into account the wide range of interferon stimulated genes that may be induced by the STING pathway, these results have broad implications in fields such as immunology, cancer biology, metabolism and stem cell research. Further, this study sets a precedent in the field of gene-engineering, possibly applicable to other enzymatic-based genome editing technologies

Structural requirements for PACSIN/Syndapin operation during zebrafish embryonic notochord development.

PACSIN/Syndapin proteins are membrane-active scaffolds that participate in endocytosis. The structure of the Drosophila Syndapin N-terminal EFC domain reveals a crescent shaped antiparallel dimer with a high affinity for phosphoinositides and a unique membrane-inserting prong upon the concave surface. Combined structural, biochemical and reverse genetic approaches in zebrafish define an important role for Syndapin orthologue, Pacsin3, in the early formation of the notochord during embryonic development. In pacsin3-morphant embryos, midline convergence of notochord precursors is defective as axial mesodermal cells fail to polarize, migrate and differentiate properly. The pacsin3 morphant phenotype of a stunted body axis and contorted trunk is rescued by ectopic expression of Drosophila Syndapin, and depends critically on both the prong that protrudes from the surface of the bowed Syndapin EFC domain and the ability of the antiparallel dimer to bind tightly to phosphoinositides. Our data confirm linkage between directional migration, endocytosis and cell specification during embryonic morphogenesis and highlight a key role for Pacsin3 in this coupling in the notochord

Venus Evolution Through Time: Key Science Questions, Selected Mission Concepts and Future Investigations

In this work we discuss various selected mission concepts addressing Venus evolution through time. More specifically, we address investigations and payload instrument concepts supporting scientific goals and open questions presented in the companion articles of this volume. Also included are their related investigations (observations & modeling) and discussion of which measurements and future data products are needed to better constrain Venus’ atmosphere, climate, surface, interior and habitability evolution through time. A new fleet of Venus missions has been selected, and new mission concepts will continue to be considered for future selections. Missions under development include radar-equipped ESA-led EnVision M5 orbiter mission (European Space Agency 2021), NASA-JPL’s VERITAS orbiter mission (Smrekar et al. 2022a), NASA-GSFC’s DAVINCI entry probe/flyby mission (Garvin et al. 2022a). The data acquired with the VERITAS, DAVINCI, and EnVision from the end of this decade will fundamentally improve our understanding of the planet’s long term history, current activity and evolutionary path. We further describe future mission concepts and measurements beyond the current framework of selected missions, as well as the synergies between these mission concepts, ground-based and space-based observatories and facilities, laboratory measurements, and future algorithmic or modeling activities that pave the way for the development of a Venus program that extends into the 2040s (Wilson et al. 2022)

Early habitability and crustal decarbonation of a stagnant-lid Venus

Little is known about the early evolution of Venus and a potential habitable period during the first 1 billion years. In particular, it remains unclear whether or not plate tectonics and an active carbonate-silicate cycle were present. In the presence of liquid water but without plate tectonics, weathering would have been limited to freshly produced basaltic crust, with an early carbon cycle restricted to the crust and atmosphere. With the evaporation of surface water, weathering would cease. With ongoing volcanism, carbonate sediments would be buried and sink downwards. Thereby, carbonates would heat up until they become unstable and the crust would become depleted in carbonates. With urn:x-wiley:21699097:media:jgre21738:jgre21738-math-0001 supply to the atmosphere the surface temperature rises further, the depth below which decarbonation occurs decreases, causing the release of even more urn:x-wiley:21699097:media:jgre21738:jgre21738-math-0002. We assess the habitable period of an early stagnant-lid Venus by employing a coupled interior-atmosphere evolution model accounting for urn:x-wiley:21699097:media:jgre21738:jgre21738-math-0003 degassing, weathering, carbonate burial, and crustal decarbonation. We find that if initial surface conditions allow for liquid water, weathering can keep the planet habitable for up to 900 Myr, followed by evaporation of water and rapid crustal carbonate depletion. For the atmospheric urn:x-wiley:21699097:media:jgre21738:jgre21738-math-0004 of stagnant-lid exoplanets, we predict a bimodal distribution, depending on whether or not these planets experienced a runaway greenhouse in their history. Planets with high atmospheric urn:x-wiley:21699097:media:jgre21738:jgre21738-math-0005 could be associated with crustal carbonate depletion as a consequence of a runaway greenhouse, whereas planets with low atmospheric urn:x-wiley:21699097:media:jgre21738:jgre21738-math-0006 would indicate active silicate weathering and thereby a habitable climate.DFG, 313698196, SPP 1992: Exploration der Diversität extrasolarer PlanetenDFG, 280637173, FOR 2440: Materie im Inneren von Planeten - Hochdruck-, Planeten- und Plasmaphysi