Strategic Alliances And New Product Development In High-Tech Ventures: The Moderating Role Of Alliance Type And Alliance Capability

Strategy scholars have proposed that capacity for managing alliance can be a source of superior performance. This study focuses on the role of this capacity, and investigates how alliance management capability of entrepreneurial firms affects the relationship between a firm’s allying and its performance. Because the capability is inherently unobservable, we take alliance experience and average duration of each alliance as proxy variables for measuring alliance management capability. An analysis of multiple allies of entrepreneurial ventures in Korean photovoltaic industry indicate that capacity for managing varying allies, and alliance type positively moderate the relation between alliance and its innovation outcomes

Gender differences in participatory leadership: An examination of principals’ time spent working with others

The purpose of this study was to examine whether female principals have a more participatory style compared to their male counterparts by examining principals’ daily time allocation patterns. The study analyzed data from End of Day (EOD) survey logs from principals in an urban school district. Results from hierarchical linear modeling (HLM) results showed that female principals, when compare to male principals, spent a higher proportion of their time working with others in planning/setting goals. At the same time, there were no differences in how principals allocated their time total working alone or working with others and their time distribution in other leadership domains. The findings suggest that gender differences in leadership style depend on specific activity domains and that there are significant differences in the key domain of strategic planning

Actin Cytoskeleton and Golgi Involvement in Barley stripe mosaic virus Movement and Cell Wall Localization of Triple Gene Block Proteins.

Barley stripe mosaic virus (BSMV) induces massive actin filament thickening at the infection front of infected Nicotiana benthamiana leaves. To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene block (TGB) proteins were coexpressed in cells with the actin marker DsRed: Talin. TGB ectopic expression experiments revealed that TGB3 is a major elicitor of filament thickening, that TGB2 resulted in formation of intermediate DsRed:Talin filaments, and that TGB1 alone had no obvious effects on actin filament structure. Latrunculin B (LatB) treatments retarded BSMV cell-to-cell movement, disrupted actin filament organization, and dramatically decreased the proportion of paired TGB3 foci appearing at the cell wall (CW). BSMV infection of transgenic plants tagged with GFP-KDEL exhibited membrane proliferation and vesicle formation that were especially evident around the nucleus. Similar membrane proliferation occurred in plants expressing TGB2 and/or TGB3, and DsRed: Talin fluorescence in these plants colocalized with the ER vesicles. TGB3 also associated with the Golgi apparatus and overlapped with cortical vesicles appearing at the cell periphery. Brefeldin A treatments disrupted Golgi and also altered vesicles at the CW, but failed to interfere with TGB CW localization. Our results indicate that actin cytoskeleton interactions are important in BSMV cell-to-cell movement and for CW localization of TGB3

Heterologous expression of a tannic acid-inducible laccase3 of Cryphonectria parasitica in Saccharomyces cerevisiae

<p>Abstract</p> <p>Background</p> <p>A tannic acid-inducible and mycoviral-regulated laccase3 (<it>lac</it>3) from the chestnut blight fungus <it>Cryphonectria parasitica </it>has recently been identified, but further characterization was hampered because of the precipitation of protein products by tannic acid supplementation. The present study investigated the heterologous expression of the functional laccase3 using a yeast <it>Saccharomyces cerevisiae</it>.</p> <p>Results</p> <p>Laccase activity in the culture broth of transformants measured using a laccase-specific substrate suggested that the <it>lac</it>3 gene was successfully expressed and the corresponding protein product secreted into the culture media. In addition, activity staining and Western blot analysis of a native gel revealed that the enzyme activity co-existed with the protein product specific to anti-laccase3 antibody, confirming that the cloned <it>lac</it>3 gene is responsible for the laccase activity. When transformants were grown on plates containing tannic acid-supplemented media, brown coloration was observed around transformed cells, indicating the oxidation of tannic acid. However, the enzymatic activity was measurable only in the selective ura^-media and was negligible in nonselective nutrient-rich culture conditions. This was in part because of the increased plasmid instability in the nonselective media. Moreover, the protein product of <it>lac</it>3 appears to be sensitive to the cultured nonselective nutrient-rich broth, because a rapid decline in enzymatic activity was observed when the cultured broth of ura^-media was mixed with that of nonselective nutrient-rich broth. In addition, constitutive expression of the <it>lac</it>3 gene resulted in a reduced cell number of the <it>lac</it>3 transformants compared to that of vector-only transformed control. However, the presence of recombinant vector without <it>lac</it>3 induction did not affect the growth of transformants.</p> <p>Conclusions</p> <p>The results suggest that expression of the <it>lac</it>3 gene has an inhibitory effect on the growth of transformed <it>S. cerevisiae </it>and that the controlled expression of <it>lac</it>3 is appropriate for the possible application of recombinant yeast to the treatment of phenolic compounds.</p

Skin care benefits of bioactive compounds isolated from Zanthoxylum piperitum DC. (Rutaceae)

Purpose: To investigate skin care efficacies of Zanthoxylum pipetitum extract and isolated compounds. Methods: Ethanol extracts of leaves, branches and fruits of what were partitioned into n-hexane, chloroform, ethyl acetate, n-butanol and aqueous layers and some fractions were further analyzed to isolate five compounds. The isolated compounds were identified based on the proton and carbon nuclear magnetic resonance (NMR) spectra. Cosmetic efficacy tests of the extracts and isolated compounds were evaluated by in vitro tests. Results: Phytochemical studies of the chloroform and ethyl acetate layers led to the isolation of five compounds; quercitrin (1), afzelin (2), hydroxy-α-sanshool (3), α-sanshool (4) and hyperoside (5). In activity tests, the extracts showed inhibitory activity against inflammation response and melanin synthesis, and induction of procollagen type I C-peptide (PIP). Among the isolated compounds, hydroxy-α-sanshool (3) and α-sanshool (4) displayed significant anti-inflammatory activity. Conclusion: The results demonstrate that Z. piperitum extract and its active compounds possess a significant potential as a cosmeeutical agent for enhancing skin quality

Textile Relics Conservation and Effective Cleaning Methods - Conservation of the Korean Youth Baseball Tournament Championship Flag

This study analyzes the effectiveness of the cleaning methods applied to the Korean Youth Baseball Tournament championship flag, Korean cultural asset no. 498, which is currently owned by the Korean Sports Council. This championship flag was first given to the winning baseball team during the Chosun National Athletic Meet in July, 1920 and therefore carries importance in Korean sports history. The conservation process began with the following goals: to understand the current condition of the relic, to reduce the soils, to repair and reinforce the deteriorated areas, and to prevent further major damages to the relic

Differential Association of Uncoupling Protein 2 Polymorphisms with Pattern Identification among Korean Stroke Patients: A Diagnostic System in Traditional Korean Medicine

Uncoupling protein 2 (UCP2), a mitochondrial protein present in many organs and cell types, is known to dissipate the proton gradient formed by the electron transport chain. Its function is correlated with predictive parameters, such as obesity, diabetes, and metabolic syndromes. We analyzed the distribution of UCP2 polymorphisms in stroke patients diagnosed with one of the following four stroke subtypes based on the TKM standard pattern identification (PI): Qi-deficiency (QD), Dampness and Phlegm (D&P), Yin-deficiency (YD), and Fire and Heat (F&D). We studied a total of 1,786 stroke patients (397/QD, 645/D&P, 223/YD, and 522/F&D, 586/normal). Genotyping for the G-1957A, G-866A and A55V UCP2 polymorphisms was performed using the TaqMan. G-866A and A55V were significantly associated with the D&P and H&F subtypes. The frequency of subjects with the A allele of G-866A was significantly lower than the frequency of subjects with the GG type. The A55V polymorphism was also shown similar effect with G-866A in the dominant model. In contrast, no SNPs were shown to be associated with the QD or YD subtypes in this study. These results showed that the G-866A and A55V UCP2 polymorphisms may be genetic factors for specific PI types among Korean stroke patients

Development of a high yield purification process for the production of influenza virus vaccines

Production of influenza virus in animal cells has emerged as an alternative to conventional platforms such as egg-based production system. Animal cells, especially MDCK and VERO cell lines, are widely used as the primary production cell for influenza virus vaccine because of their high susceptibility to infection with various influenza viruses. Recently, a robust and reliable purification process was successfully developed for the production of quadri-valent HA proteins (from two strains of the type A virus and two strains of the type B virus) by using animal cell-based production system in Green Cross Corp., Korea. The UF/DF process, Benzonase treatment at high temperature as well as column chromatography strategy was optimized to maximize the final HA production yields. Benzonase treatment was conducted to reduce in hcDNA (host cell DNA) because hcDNA was main impurity for cell-based influenza virus vaccine. A simple and stable UF/DF process has been tested with membrane molecular weight cutoffs of 100 and 300 kDa as well as 0.2 and 0.45 um microfiltration membrane. Anion exchange chromatography (AEC) and size exclusion chromatography (SEC) were selected for acceptable reduction in hcDNA and HCP. AEC was used to separate hcDNA from virus at a salt concentration of 0.5 M sodium chloride. The HA yield through AEC & SEC combination process was sufficiently achieved under specific purification process condition. Overall, the amount of residual hcDNA was reduced to an acceptable level (10ng/dose) and the increased HA yield was maintained throughout the whole process. The performance, productivity and scalability of the purification process were successfully demonstrated in over 30 GMP batches using 4 different influenza virus strains