3,345 research outputs found
The PlySs9 endolysin and its amidase subdomain reveal potential roles in the treatment of Gram-positive bovine mastitis
Talking Trash: Ethics, Sustainability, And Organizational Trust
In 1960 Americans generated 88.1 million tons (2.68 lbs./person/day) of municipal solid waste (MSW), by 2012 municipal solid waste increased to 250.9 million tons (4.38 lbs./person/day). The good news is that of the total MSW 5.6 million tons was recycled in 1960 and by 2012 the amount surged to 86.6 million tons. This real-world case (to protect privacy, names have been changed) chronicles the development of a strategic supply chain decision within a heavily regulated industry that is characterized by hybrid public/private organizations. It illustrates the importance of contract compliance and quality control assessment. In addition, it is heavily imbedded within the sustainability conversation. Finally, integral elements of the case are ethics and organizational trust, which, regardless of contract obligations, is essential in reciprocal relationships and community/customer support
A Holistic Approach to Quantum Ethics Education
This paper first provides an overview of the growing subfield of quantum
ethics, including a working definition; research to date into social, economic,
and political implications of various quantum technologies; and directions for
future research. Second, it introduces the Quantum Ethics Project (QEP), its
activities to date, and its organizing philosophy. The third section reports on
QEP's ongoing curriculum development work, i.e. creating one of the first
full-length courses on Ethics and Social Impacts of Quantum Technology. We
outline the pedagogical approach being taken in the course design, including
key learning outcomes, topic areas, teaching methods, and rationale. This is
followed by a brief discussion of current limitations and future areas of
attention, such as drawbacks to teaching ethical reasoning and ideas for
assessment and implementation. Finally, the paper concludes with a call for
collaborators and information on how people can join the QEP network.Comment: This work has been submitted to the IEEE for possible publication.
Copyright may be transferred without notice, after which this version may no
longer be accessible. 10 pages, submitted to QSEEC 202
Measuring gender norms about relationships in early adolescence : results from the global early adolescent study
Introduction: Gender norms are increasingly recognized as drivers of health and wellbeing. While early adolescence constitutes a critical window of development, there is limited understanding about how adolescents perceive gender relations across different cultural settings. This study used a mixed-method approach, grounded in the voices of young people around the world, to construct and test a cross-cultural scale assessing the perceptions of gender norms regulating romantic relationships between boys and girls in early adolescence.
Methods: The study draws on the Global Early Adolescent study (GEAS), a study focusing on gender norms and health related outcomes over the course of adolescence in urban poor settings worldwide. In-depth interviews were first conducted among approximately 200 adolescents between 10-14 years in seven sites across 4 continents to identify common scripts guiding romantic relations in early adolescence. These scripts were then transformed into a multidimensional scale. The scale was tested among 120 adolescents in each of 14 GEAS sites, followed by a second pilot among 75 adolescents in six sites. We evaluated the psychometric criteria of each subscale using principal component analysis, and parallel analysis, followed by exploratory factor analysis to guide the selection of a more parsimonious set of items.
Results: Results suggested a two-factor structure, consisting of an "adolescent romantic expectations" subscale and a "Sexual Double Standard" subscale. Both subscales yielded high internal validity in each site, with polychoric Cronbach alpha values above 0.70 with the exception of Kinshasa for the adolescent romantic expectations scale (0.64) and Hanoi for the sexual double standard scale (0.61).
Conclusion: This study reveals common perceptions of gendered norms about romantic engagement in early adolescence, normative for both sexes, but socially valued for boys while devaluated for girls. The findings illustrate that social hierarchies of power in romantic relationships form early in adolescence, regardless of cultural setting
Carbon and Nitrogen Isotopes in Type II Supernova Diamonds
We construct a model for the origin of the abundant nanometer diamonds found in meteorites. We interpret them as interstellar particles that were grown during the expansions of supernova interiors. The physical setting and the chemical-vapor-deposition process that we describe present clear reasons both for the small sizes (nm) of the diamonds and for their mean isotopic composition being not greatly different from solar. To delineate the isotopic requirements for collections of diamonds (which are too small for analysis individually) we present measurements of carbon and nitrogen isotopes obtained by stepped combustion of diamond collections. Our model for the growth of supernova diamonds is motivated by a series of postulates, unremarkable as single postulates, that together produce a successful calculation. The computed growth occurs in the continuously mixing envelopes of expanding Type II supernova remnants. It provides a good characterization of these facts: (1) the C isotopic composition is not far from solar; (2) both carbon and nitrogen become isotopically lighter as the diamonds are combusted; (3) the C/N ratio changes during combustion; (4) the diamonds are individually tiny; (5) collections of diamonds are carriers of Xe-HL. We show that the isotopic gradient during combustion may be interpreted in this model as either an isotopic gradient within each diamond or as a correlation between isotopic composition and size of individual diamonds contained in the bulk collections
Characterization of the bacteriophage-derived endolysins PlySs2 and PlySs9 with in vitro lytic activity against bovine mastitis streptococcus uberis
Bovine mastitis, an infection of the cow’s mammary gland, is frequently caused by Streptococcus uberis and causes major economic losses in the dairy industry. The intramammary administration of antibiotics currently remains the predominant preventive and therapeutic measure. These antimicrobial compounds, ofwhich some are consideredcritical in human health care, are frequently applied as dry therapy resulting in their consistent overuse. Therefore, the use of antibiotics in the dairy sector is being questioned. We here identified two endolysins, i.e., PlySs2 and PlySs9, respectively derived from Streptococcus suis serotype-2 and -9 prophages, with lytic activity against S. uberis in an in vitro setting. Both endolysins gave clear lysis zones in spot-on-plate assays and caused a reduction of the optical density in a turbidity reduction assay. In depth characterization identified PlySs9 as the more potent endolysin over PlySs2 with a lower MIC value and about one additional log of killing. PlySs2 and PlySs9 were challenged to a panel of subclinical and clinical S. uberis milk isolates and were both able to lyse all strains tested. Molecular dissection of these endolysins in catalytic and cell wall binding subdomains resulted in major loss of killing and binding activity, respectively. Taken together, we here propose PlySs2 and PlySs9 as candidate compounds to the current antimicrobial arsenal known against bovine mastitis-causing S. uberis as future add-on or replacement strategy to the currently used intramammary antibiotics
Anchoring of a dye precursor on NiO(001) studied by non-contact atomic force microscopy
The properties of metal oxides, such as charge transport mechanisms or optoelectronic characteristics, can be modified by functionalization with organic molecules. This kind of organic/inorganic surface is nowadays highly regarded, in particular, for the design of hybrid devices such as dye sensitized solar cells. However, a key parameter for optimized interfaces is not only the choice of the compounds but also the properties of adsorption. Here, we investigated the deposition of an organic dye precursor molecule on a NiO(001) single crystal surface by means of non-contact atomic force microscopy at room temperature. Depending on the coverage, single molecules, groups of adsorbates with random or recognizable shapes, or islands of closely packed molecules were identified. Single molecules and self assemblies are resolved with sub-molecular resolution showing that they are lying flat on the surface in a trans-conformation. Within the limits of our Kelvin probe microscopy setup a charge transfer from NiO to the molecular layer of 0.3 electrons per molecules was observed only in the areas, where the molecules are closed packed
‘Question Moments’: A Rolling Programme of Question Opportunities in Classroom Science
This article has been made available through the Brunel Open Access Publishing Fund.This naturalistic study integrates specific 'question moments' into lesson plans to
increase pupils' classroom interactions. A range of teaching tools has explored
students' ideas through opportunities to ask and write questions. Their oral and written
outcomes provide data on individual and group misunderstandings. Changes to the
schedule of lessons were introduced to discuss these questions and solve disparities.
Flexible lesson planning over fourteen lessons across a four-week period of highschool
chemistry accommodated students' contributions and increased student
participation, promoted inquiring and individualised teaching, with each teaching
strategy feeding forward into the next
Characterization of engraftment dynamics in myelofibrosis after allogeneic hematopoietic cell transplantation including novel conditioning schemes.
INTRODUCTION
Myelofibrosis (MF) is a rare hematopoietic stem cell disorder progressing to bone marrow (BM) failure or blast phase. Allogeneic hematopoietic cell transplantation (HCT) represents a potentially curative therapy for a limited subset of patients with advanced MF, who are eligible, but engraftment in MF vs. AML is delayed which promotes complications. As determinants of engraftment in MF are incompletely characterized, we studied engraftment dynamics at our center.
METHODS
A longitudinal cohort of 71 allogeneic HCT performed 2000-2019 with >50% after 2015 was evaluated.
RESULTS
Median time to neutrophil engraftment ≥0.5x109/l was +20 days post-transplant and associated with BM fibrosis, splenomegaly and infused CD34+ cell number. Engraftment dynamics were similar in primary vs. secondary MF and were independent of MF driver mutations in JAK2, CALR and MPL. Neutrophil engraftment occurred later upon haploidentical HCT with thiotepa-busulfan-fludarabine conditioning, post-transplant cyclophosphamide and G-CSF (TBF-PTCy/G-CSF) administered to 9.9% and 15.6% of patients in 2000-2019 and after 2015, respectively. Engraftment of platelets was similarly delayed, while reconstitution of reticulocytes was not affected.
CONCLUSIONS
Since MF is a rare hematologic malignancy, this data from a large number of HCT for MF is essential to substantiate that later neutrophil and platelet engraftment in MF relates both to host and treatment-related factors. Observations from this longitudinal cohort support that novel conditioning schemes administered also to rare entities such as MF, require detailed evaluation in larger, multi-center cohorts to assess also indicators of long-term graft function and overall outcome in patients with this infrequent hematopoietic neoplasm undergoing allogeneic transplantation
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