إنتاج مادة 17 ألفا هيدروكسي البروجستيرون على مستوى المخمر المعملي بواسطة فطرة كاننجهاميلا إيكينولاتا

The mircrobiological transformation of progesterone by a local isolate of Cunninghamella echiiiulata using a laboratory fermentor was studied. Progresterone (10-50 g/1) wetted by Tween 80 was added to 48-hour old culture and the transformation was left to proceed for 72 hours. Thereafter, the different transformation products were resolved chromatog-raphically. The identity of each product was established through the determination of m.p., mixed m.p., optical rotation and ultraviolet as well as infrared absorption spectra. A comparison of the R{ values of each product with that of the corresponding reference using different solvent systems as well as their colour expressed with two spray reagents, was used as a further proof for the identity of the isolated products. With all concentrations of progesterone tested, maximum yield of 17ot -hydroxyprogesterone was obtained after 48 hours of fermentation Progesterone concentrations of 10 and 20 g/1 were almost quantitatively converted to the different transformation products after 72 hours of fermentation. Using a concentration of 20 g/1 and incubation period of 48 hours, the transformation product mixture consisted of unchanged progesterone (6%), 17 o< -hydroxyprogesterone (54%),llotrhydroxyprogesterone (29%) and llo<;,17<^-dihydroxy-progesterone (2.5%).تم استخدام مخمر صناعي سعة 2 لتر لاختيار مقدرة الفطرة على تكوين هذه المادة في ظروف تشبه تلك المطبقة في الصناعة . وبدراسة تركيزات متعددة فن مادة البروجستيرون تتراوح ما بين 10جرام /لتر إلى 50جرام /لتر ، وجد أن أنسب التركيزات المختبرة هو تركيز 20 جرام من البروجستيرون لكل لترمن الوسط الغذائي ، حيث تم تحويل كل البووجستيرون المضاف إلى المشتقات المختلفة خلال 72 ساعة من بدء الاضافة . ووجد أن أعلى معدل لتكوين مادة 17 ألفا - هيدروكسي البروجستيرون كان بعد 48 ساعة من بدأ إضافة البروجستيرون . عند فصل المواد الناتجة من تحول البروجستيرون بواسطة الفطرة المستخدمة وذلك بواسطة أعمدة الفصل باستخدام مادة الالومينا وجد أن البروجستيرون يتحول إلى : 17 ألفا - هيدروكسي البروجستيرون ( 54 %) 11 ألفا - هيدروكسي البروجستيرون (29%) 11 ألفا ، 17 ألفا - ثنائي هيدروكسي البروجستيرون (2.5%

Existence of monotonic LφL_\varphi-solutions for quadratic Volterra functional-integral equations

We study the quadratic integral equation in the space of Orlicz space $E_{\varphi}$ in the most important case when $\varphi$ satisfies the $\Delta_2$-condition. Considered operators are not compact and then we use the technique of measure of noncompactness associated with the Darbo fixed point theorem to prove the existence of a monotonic, but discontinuous solution. Our present work allows to generalize both previously proved results for quadratic integral equations as well as that for classical equations. Due to different continuity properties of considered operators in Orlicz spaces, we distinguish different cases and we study the problem in the most important case – in such a way to cover all Lebesgue spaces $L_p$ ($p \geq 1$)

Impact of application of zinc oxide nanoparticles on callus induction, plant regeneration, element content and antioxidant enzyme activity in tomato (Solanum lycopersicum MILL) under salt stress

The properties of nanomaterials and their potential applications have been given considerable attention by researchers in various fields, especially agricultural biotechnology. However, not much has been done to evaluate the role or effect of zinc oxide nanoparticles (ZnO-NP) in regulating physiological and biochemical processes in response to salt-induced stress. For this purpose, some callus growth traits, plant regeneration rate, mineral element (sodium, potassium, phosphorous and nitrogen) contents and changes in the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in tissues of five tomato cultivars were investigated in a callus culture exposed to elevated concentrations of salt (3.0 and 6.0 g L-1NaCl), and in the presence of zinc oxide nanoparticles (15 and 30 mg L-1). The relative callus growth rate was inhibited by 3.0 g L-1 NaCl; this was increased dramatically at 6.0 g L-1. Increasing exposure to NaCl was associated with a significantly higher sodium content and SOD and GPX activities. Zinc oxide nanoparticles mitigated the effects of NaCl, and in this application of lower concentrations (15 mg L-1) was more effective than a higher concentration (30 mg L-1). This finding indicates that zinc oxide nanoparticles should be investigated further as a potential anti-stress agent in crop production. Different tomato cultivars showed different degrees of tolerance to salinity in the presence of ZnO-NP. The cultivars Edkawy, followed by Sandpoint, were less affected by salt stress than the cultivar Anna Aasa

Implications of automated vehicles for street design and planning: Espoo case

Automated Vehicles (AVs), in their foundational stage, are gradually emerging into Espoo’s road network. During the transition phase, AVs are expected to introduce several challenges and requirements for road operators in design and maintenance of physical infrastructure. This has pushed cities to investigate the potential changes needed to the way their road networks are operated and managed, to consistently support and optimize the outcomes from the introduction of AVs. The thesis uses a combination of qualitative methods, including map-based survey, road test drives, expert discussions and critical testing scenarios to identify and assess several street design elements in Espoo. The study assesses the automation ability of the Tesla Autopilot in the road network by experimenting several driving scenarios and weather conditions i.e. night and rain. The study also briefly tests other steering assist systems as a way to assess and compare capabilities of other steering assist systems within similar road environments. Today, the design and quality of road markings are the key features influencing the operation of machine vision based automated systems. Therefore, discussions regarding street design implications are mainly related to the design of longitudinal markings. In this study, several design elements had been identified and studied, including edge marking, lane split and merge marking, bus stop and side parking marking. Based on the current technological trends in vehicle automation, road operators are advised to consider several physical infrastructure and maintenance elements, including primarily the machine readability of line markings. The consistency in design, implementation and maintenance of road markings are seen to have the most benefit in facilitating the deployment of AVs today. However, it was observed that some road marking elements were more critical than others, and therefore, it is suggested that they have higher maintenance and design priority. While the study assesses street design elements that are seen significant for the operation of steering assist systems today, operators are advised to consider planning frameworks to plan for the introduction of AVs, in order to avoid making changes that may hinder their operation in the future. However, it is important to consider other aspects of road operation and management when considering any new innovative changes in street design in the future