A damaging block-based model for the analysis of the cyclic behaviour of full-scale masonry structures

In this paper, a damaging block-based model is proposed for the numerical analysis of the cyclic behaviour of full-scale masonry structures. Solid 3D finite elements governed by a plastic-damage constitutive law in tension and compression are used to model the blocks, while a cohesive-frictional contact-based formulation is developed to simulate their cyclic interaction. The use of tests on small-scale specimens to calibrate the mechanical properties of the numerical model is presented and discussed. The tests belong to a comprehensive experimental campaign performed on calcium silicate brick masonry. The calibrated models are used to simulate in-plane and out-of-plane cyclic tests on masonry walls made of the same material, as well as a quasi-static cyclic pushover test on a full-scale terraced masonry house. The efficiency, the potentialities and the accuracy of the model here proposed are shown and discussed. The capability of explicitly representing structural details (e.g. running bonds) and any in-plane and through-thickness texture of masonry, which appears essential to study the response of masonry structures, is guaranteed by the block-based modelling approach. A good agreement between the numerical results and the experimental outcomes is observed. This allows to validate the model in the cyclic response as well as the strategy proposed for its mechanical characterization

Human metapneumovirus establishes persistent infection in lung microvascular endothelial cells and primes a th2-skewed immune response

Human metapneumovirus (HMPV) is a major cause of lower respiratory tract infections. HMPV infection has been hypothesized to alter dendritic cell (DC) immune response; however, many questions regarding HMPV pathogenesis within the infected lung remain unanswered. Here, we show that HMPV productively infects human lung microvascular endothelial cells (L-HMVECs). The release of infectious virus occurs for up to more than 30 days of culture without producing overt cytopathic effects and medium derived from persistently HMPV-infected L-HMVECs (secretome) induced monocyte-derived DCs to prime naïve CD4 T-cells toward a Th2 phenotype. Moreover, we demonstrated that infected secretomes trigger DCs to up-regulate OX40L expression and OX40L neutralization abolished the pro-Th2 effect that is induced by HMPV-secretome. We clarified secretome from HMPV by size exclusion and ultracentrifugation with the aim to characterize the role of viral particles in the observed pro-Th2 effect. In both cases, the percentage of IL-4-producing cells and expression of OX40L returned at basal levels. Finally, we showed that HMPV, per se, could reproduce the ability of secretome to prime pro-Th2 DCs. These results suggest that HMPV, persistently released by L-HMVECs, might take part in the development of a skewed, pro-Th2 lung microenvironment

Hair analysis for detection of triptans occasionally used or overused by migraine patients-a pilot study

Purpose The aim of this study is to evaluate the detection rate of almotriptan, eletriptan, frovatriptan, sumatriptan, rizatriptan, and zolmitriptan in the hair of migraineurs taking these drugs; the degree of agreement between type of self-reported triptan and triptan found in hair; if the concentrations in hair were related to the reported cumulative doses of triptans; and whether hair analysis was able to distinguish occasional use from the overuse of these drugs. Methods Out of 300 headache patients consecutively enrolled, we included 147 migraine patients who reported to have taken at least one dose of one triptan in the previous 3 months; 51 % of the patients overused triptans. A detailed pharmacological history and a sample of hair were collected for each patient. Hair samples were analyzed by liquid chromatography-electrospray tandem mass spectrometry (LC-MS/MS) by a method that we developed. Results All the triptans could be detected in the hair of the patients. The agreement between type of self-reported triptan and type of triptan found in hair was from fair to good for frovatriptan and zolmitriptan and excellent for almotriptan, eletriptan, sumatriptan, and rizatriptan (P < 0.01, Cohen’s kappa). The correlation between the reported quantities of triptan and hair concentrations was statistically significant for almotriptan, eletriptan, rizatriptan, and sumatriptan (P < 0.01, Spearman’ s rank correlation coefficient). The accuracy of hair analysis in distinguishing occasionally users from overusers was high for almotriptan (ROC AUC = 0.9092), eletriptan (ROC AUC = 0.8721), rizatriptan (ROC AUC = 0.9724), and sumatriptan (ROC AUC = 0.9583). Conclusions Hair analysis can be a valuable system to discriminate occasional use from triptan overuse

Manual therapy for chronic migraine: a pragmatic randomised controlled trial study protocol

Introduction Chronic migraine is a largely refractory condition affecting between 1 and 2.2% of the overall population worldwide, with females more affected than males. There are also high health and socioeconomic costs associated both for the individual and society. The mainstay of chronic migraine management is pharmacological, but the options available have limited efficacy and there are often unwanted side effects. There is some evidence for manual therapy as a treatment option for migraine, but its effectiveness for chronic migraine is unknown. Therefore, we have designed a pragmatic randomised control trial to investigate whether adding manual therapy to the tertiary specialist treatment of chronic migraine improves patient-reported outcomes. Methods A pragmatic, randomised controlled trial in a hospital tertiary headache clinic. Participants will be randomised into one of two groups: treatment as usual or treatment as usual plus manual therapy. The primary outcome measure will be a change in the Headache Impact Test score. Secondary outcomes will also be measured over the 12-week study period including changes in headache frequency, migraine specific quality of life and reductions in relevant medicine consumption. The manual therapy group will have five treatment sessions each lasting 30 min. The recruitment target of 64 participants will allow power at 80% with p = 0.05 using minimal clinical difference for Headache Impact Test of 3.7 and includes provision for a 10% dropout rate. Recruitment will take place between August 2018 and February 2019. The results will form part of a doctoral study and be published in peer-reviewed journals and presented at national/international conferences. Discussion Current pharmacological approaches have limited effects in the management of chronic migraine and there is a requirement to improve treatment options and reduce the health and economic burden of the condition. Manual therapy has been shown to be effective in other chronic pain conditions as well as other primary headaches. This study will explore the effectiveness of manual therapy as an adjunctive approach to the management of chronic migraine. Trial registration The trial has received a favourable opinion from the UK Health Research Authority (IRAS 228901) and is registered at ClinicalTrials.gov.number NCT03395457. Registered 1st March 2018

Virucidal efficacy of a novel silver-based disinfectant against SARS-CoV-2 Omicron BA.5

In this study we evaluated the antiviral activity of the Silver Barrier® disinfectant against SARSCoV-2. Silver Barrier® showed time- and concentration-dependent antiviral activity against SARSCoV-2. After 5 min contact time, Silver Barrier® at 0.002% showed a strong inhibitory effect (p<0.001), with a 2-fold reduction of viral genome copy numbers, and a robust suppression (94%) of SARS-CoV-2 infectivity. Considering the effects obtained in solution and within a very short time, Silver Barrier® stands as an excellent new candidate for the disinfection of work environments, especially at the healthcare level, where there are people at high risk of serious illnesses

A novel tier-based numerical analysis procedure for the structural assessment of masonry quay walls under traffic loads

Historical quay walls constructed in unreinforced masonry are integral elements of many cities. Originally designed as gravity retaining walls, they are nowadays often subjected to the action of traffic loads as a result of vehicles travelling on roads constructed on their backfill. This paper presents a numerical analysis procedure for carrying out the structural assessment of quay walls under traffic loads. The procedure simulates the non-linear dynamic response of the quay wall under the effect of the passage of a vehicle. Non-linear dynamic calculations are performed not only to be representative of the actual nature of loading but also to produce realistic estimations of structural safety, load redistribution capacities and displacements. Adopting a tier-based approach, the computational burden typically associated with such simulations is significantly reduced. This is obtained by adopting simplifications which allow for the modelling the 3D soil block comprising the backfill of the quay wall only in the first tier of the procedure. To demonstrate the implementation of the procedure, a detailed application to an existing quay wall in Amsterdam, the Netherlands, is presented. Different foundation damage scenarios are also considered. Though the procedure is presented in this paper for a specific typology of quay walls, it has conceptual and methodological value. With appropriate modifications it can be used for the structural assessment of other earth retaining structures as well, under the effect of vehicular traffic on their backfills