Factors Affecting Sustainability Of Wetland Agriculture Within Lake Victoria Basin In Uganda

In recent years, the high rate of conversion of wetlands for agriculture has raised environmental concerns in Uganda. A study was therefore conducted to identify issues that need to be addressed if communities are to continue deriving livelihoods from wetland agriculture, without causing stress to the wetlands of Lake Victoria basin. This was done by conducting household surveys and focus group discussions in two of the districts that border Lake Victoria. Wetland agriculture was found to be a significant source of livelihoods for resource poor communities, contributing over 60% of the household income. Farming was mainly done at a subsistence level. The study found that socio economic factors, poor farming practices and weak policies were contributing to the rapid conversion of wetlands. Concerted efforts need to be made by government institutions to sensitize communities on conservation of wetlands. Some of the policies need to be harmonized to avoid contradictions. Alternative sources of livelihoods must be created to ensure socio economic benefits and environmental conservation. Keywords: Wetland agriculture; Sustainability; Livelihoods; Uganda.Journal of Agriculture and Social Research Vol. 8 (1) 2008: pp. 78-8

FAK acts as a suppressor of RTK-MAP kinase signalling in Drosophila melanogaster epithelia and human cancer cells

Receptor Tyrosine Kinases (RTKs) and Focal Adhesion Kinase (FAK) regulate multiple signalling pathways, including mitogen-activated protein (MAP) kinase pathway. FAK interacts with several RTKs but little is known about how FAK regulates their downstream signalling. Here we investigated how FAK regulates signalling resulting from the overexpression of the RTKs RET and EGFR. FAK suppressed RTKs signalling in Drosophila melanogaster epithelia by impairing MAPK pathway. This regulation was also observed in MDA-MB-231 human breast cancer cells, suggesting it is a conserved phenomenon in humans. Mechanistically, FAK reduced receptor recycling into the plasma membrane, which resulted in lower MAPK activation. Conversely, increasing the membrane pool of the receptor increased MAPK pathway signalling. FAK is widely considered as a therapeutic target in cancer biology; however, it also has tumour suppressor properties in some contexts. Therefore, the FAK-mediated negative regulation of RTK/MAPK signalling described here may have potential implications in the designing of therapy strategies for RTK-driven tumours

Fission of Tubular Endosomes Triggers Endosomal Acidification and Movement

The early endosome acts as a sorting station for internalized molecules destined for recycling or degradation. While recycled molecules are sorted and delivered to tubular endosomes, residual compartments containing molecules to be degraded undergo “maturation” before final degradation in the lysosome. This maturation involves acidification, microtubule-dependent motility, and perinuclear localization. It is currently unknown how sorting and the processes of maturation cooperate with each other. Here, we show that fission of a tubular endosome triggers the maturation of the residual endosome, leading to degradation. Use of the dynamin inhibitor dynasore to block tubular endosome fission inhibited acidification, endosomal motility along microtubules, perinuclear localization, and degradation. However, tubular endosome fission was not affected by inhibiting endosomal acidification or by depolymerizing the microtubules. These results demonstrate that the fission of recycling tubules is the first important step in endosomal maturation and degradation in the lysosome. We believe this to be the first evidence of a cascade from sorting to degradation

Receptor Sorting within Endosomal Trafficking Pathway Is Facilitated by Dynamic Actin Filaments

Early endosomes (EEs) are known to be a sorting station for internalized molecules destined for degradation, recycling, or other intracellular organelles. Segregation is an essential step in such sorting, but the molecular mechanism of this process remains to be elucidated. Here, we show that actin is required for efficient recycling and endosomal maturation by producing a motile force. Perturbation of actin dynamics by drugs induced a few enlarged EEs containing several degradative vacuoles and also interfered with their transporting ability. Actin repolymerization induced by washout of the drug caused the vacuoles to dissociate and individually translocate toward the perinuclear region. We further elucidated that cortactin, an actin-nucleating factor, was required for transporting contents from within EEs. Actin filaments regulated by cortactin may provide a motile force for efficient sorting within early endosomes. These data suggest that actin filaments coordinate with microtubules to mediate segregation in EEs

An act of agency: people with albinism in Tanzania creating change

The contact strategy has proven to be effective in reducing stigma in relation a variety of disabilities and health-related conditions. This strategy requires people to be willing to present on their condition. This study explored what the considerations are of ‘spokespersons’ to do so. Semi-structured in-depth interviews were conducted. Informants (13) expressed how they felt about presenting, that it gave them the opportunity to develop themselves and to increase their status and confidence. Informants felt responsible for making a change in society and experienced their presentations as an effective strategy. The informants did not consider presenting themselves as a risk, as long as the necessary security arrangements are made, and proper training is provided. Recommendations are provided for the implementation and development of contact interventions in relation to health-related stigma