87 research outputs found

    Heimkehr nach Europa: Ostmittel-, Ost- und Südosteuropa nach dem Ende des Ostwest- Konflikts

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    Black Carbon Contribution to the Aerosol Phase and its Scavenged Fraction in Mixed Phase Clouds at the High Alpine Site Jungfraujoch (3580m asl)

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    The mass fraction of black carbon (BC) in the atmospheric aerosol and its mixing state are important for the direct aerosol climate effect. These properties also determine if BC is incorporated into cloud hydrometeors (i.e. droplets and ice crystals) and are important because the microphysical and optical properties of the cloud are altered (indirect aerosol effect). Measurements were performed during several Cloud and Aerosol Characterization Experiments, in winter 2004 (CLACE3), summer 2004 (CLACE3.5), winter 2005 (CLACE4) and summer 2005 (CLACE4.5) at the high Alpine research station Jungfraujoch (3580 m asl)

    Chemical composition of ambient aerosol, ice residues and cloud droplet residues in mixed-phase clouds: single particle analysis during the Cloud and Aerosol Characterization Experiment (CLACE 6)

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    Two different single particle mass spectrometers were operated in parallel at the Swiss High Alpine Research Station Jungfraujoch (JFJ, 3580 m a.s.l.) during the Cloud and Aerosol Characterization Experiment (CLACE 6) in February and March 2007. During mixed phase cloud events ice crystals from 5–20 micro m were separated from larger ice aggregates, non-activated, interstitial aerosol particles and supercooled droplets using an Ice-Counterflow Virtual Impactor (Ice-CVI). During one cloud period supercooled droplets were additionally sampled and analyzed by changing the Ice-CVI setup. The small ice particles and droplets were evaporated by injection into dry air inside the Ice-CVI. The resulting ice and droplet residues (IR and DR) were analyzed for size and composition by the two single particle mass spectrometers: a custom-built Single Particle Laser-Ablation Time-of-Flight Mass Spectrometer (SPLAT) and a commercial Aerosol Time-of-Flight Mass Spectrometer (ATOFMS, TSI Model 3800). During CLACE 6 the SPLAT instrument characterized 355 individual IR that produced a mass spectrum for at least one polarity and the ATOFMS measured 152 IR. The mass spectra were binned in classes, based on the combination of dominating substances, such as mineral dust, sulfate, potassium and elemental carbon or organic material. The derived chemical information from the ice residues is compared to the JFJ ambient aerosol that was sampled while the measurement station was out of clouds (several thousand particles analyzed by SPLAT and ATOFMS) and to the composition of the residues of supercooled cloud droplets (SPLAT: 162 cloud droplet residues analyzed, ATOFMS: 1094). The measurements showed that mineral dust was strongly enhanced in the ice particle residues. Close to all of the SPLAT spectra from ice residues did contain signatures from mineral compounds, albeit connected with varying amounts of soluble compounds. Similarly, close to all of the ATOFMS IR spectra show a mineral or metallic component. Pure sulfate and nitrate containing particles were depleted in the ice residues. Sulfate and nitrate was found to dominate the droplet residues (~90% of the particles). The results from the two different single particle mass spectrometers were generally in agreement. Differences in the results originate from several causes, such as the different wavelength of the desorption and ionisation lasers and different size-dependent particle detection efficiencies

    Design of an affordable electric snowmobile -Done Right

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    ABSTRACT The University of Alaska Fairbanks Nanook EV team's latest electric snowmobile has a 51.5 km (32 mi) range at 32 km/h (20 mi/h) under optimal snow conditions. Building on the 2011 competition success (winning Best Design with a Ski-Doo Tundra 300F), we started this project with a much improved chassis: a Ski-Doo Renegade Sport 550

    Current issues in medically assisted reproduction and genetics in Europe: research, clinical practice, ethics, legal issues and policy. European Society of Human Genetics and European Society of Human Reproduction and Embryology.

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    In March 2005, a group of experts from the European Society of Human Genetics and European Society of Human Reproduction and Embryology met to discuss the interface between genetics and assisted reproductive technology (ART), and published an extended background paper, recommendations and two Editorials. Seven years later, in March 2012, a follow-up interdisciplinary workshop was held, involving representatives of both professional societies, including experts from the European Union Eurogentest2 Coordination Action Project. The main goal of this meeting was to discuss developments at the interface between clinical genetics and ARTs. As more genetic causes of reproductive failure are now recognised and an increasing number of patients undergo testing of their genome before conception, either in regular health care or in the context of direct-to-consumer testing, the need for genetic counselling and preimplantation genetic diagnosis (PGD) may increase. Preimplantation genetic screening (PGS) thus far does not have evidence from randomised clinical trials to substantiate that the technique is both effective and efficient. Whole-genome sequencing may create greater challenges both in the technological and interpretational domains, and requires further reflection about the ethics of genetic testing in ART and PGD/PGS. Diagnostic laboratories should be reporting their results according to internationally accepted accreditation standards (International Standards Organisation - ISO 15189). Further studies are needed in order to address issues related to the impact of ART on epigenetic reprogramming of the early embryo. The legal landscape regarding assisted reproduction is evolving but still remains very heterogeneous and often contradictory. The lack of legal harmonisation and uneven access to infertility treatment and PGD/PGS fosters considerable cross-border reproductive care in Europe and beyond. The aim of this paper is to complement previous publications and provide an update of selected topics that have evolved since 2005

    Robust estimation of bacterial cell count from optical density

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    Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

    How to bring Europe back to its citizens

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    This is the archive of a lecture given by Michael Mertes, State Secretary, Commissioner for Federal, European and Foreign Affairs, State Chancellery of North Rhine-Westphalia, Germany; Moderator: Krzysztof Michalski, Rector, Institute for Human Sciences, Vienna; Professor of Philosophy, Boston University

    What Distinguishes Europe?

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    The powers of cohesion in an expanded European Union must “be looked for and found in Europe’s common culture” the Reflection Group’s Europe Paper emphasizes. Indeed: “As the old forces of integration— the desire for peace, the existence of external threats, and the potential for economic growth—lose their effectiveness, the role of Europe’s common culture—the spiritual factor of European integration— will inevitably grow in importance as a source of unity and cohesion.” Whether, as stated at..

    German-American relations before and after September 11

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    This is the archive of a lecture given by Michael Mertes, partner at "dimap consult", a commercial think tank based in Bonn and Berlin; Respondent: Charles Maier, Center for European Studies, Harvard University. Listen to the lecture at https://www.bu.edu/european/2008/05/25/german-american-relations-before-and-after-september-11>
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