The effect of intrauterine thyme essential oil and dimethyl sulfoxide infusion on clinical recovery and serum haptoglobin, tumor necrosis factor and nitric oxide levels in cows with clinical endometritis

Bu çalışmada, intrauterin kekik yağı ile dimetil sülfoksit (DMSO) uygulamasının endometritisin klinik iyileşmesi, serum haptoglobin (Hp), tümör nekrozis faktör (TNF) ve nitrik oksit (NO) düzeylerine etkisini belirlemek amaçlandı. Çalışmada klinik endometritisli 90 inek rastgele 3 gruba ayrıldı. Birinci gruptaki ineklere (GI; n=30) kekik esansiyel yağı (20 mL), 12 mL DMSO ve 88 mL distile su karıştırıldı ve karışım (120 mL) intrauterin olarak verildi. İkinci gruptaki ineklere (GII; n=30) 20 mL kekik esansiyel yağı 100 mL distile su içerisine eklendi ve intrauterin olarak verildi. Son gruptaki ineklere ise (GIII; n=30) 12 mL DMSO, 108 mL distile su ile karıştırıldıktan sonra intrauterin olarak verildi. Tedaviden 14 gün sonra rektal, ultrasonografik ve vaginoskobik muayene yapıldı. Tüm hayvanlardan tedaviden hemen önce ve tedaviden 14 gün sonra kan alındı. Kan serumlarda Hp, TNF ve NO düzeyleri ELISA ticari kitleri ile belirlendi. Tüm gruplarda Hp, TNF ve NO düzeylerinin tedavi öncesine göre önemli oranda düştüğü saptandı. Klinik iyileşme oranı en yüksek GI’de (%93,3) tespit edildi. GII’de %73,3, GIII’te ise %56,6 olarak belirlendi. Sonuç olarak, intrauterin kekik esansiyel yağ tedavisi ile DMSO verilmesinin endometritisin klinik iyileşmesi üzerine pozitif yönde katkı sağladığı belirlenmiştir.This study, it was aimed to determine the effect of the intrauterine infusion of thyme essential oil and dimethyl sulphoxide (DMSO) on serum haptoglobin (Hp), tumor necrosis factor (TNF) and nitric oxide (NO) levels and on healing in cows with clinical endometritis. In the study, 90 cows with clinical endometritis were randomly assigned to three groups. Cows in the first group (GI, n=30) were treated with an intrauterine infusion of the 120 mL mixture prepared from 20 mL thyme essential oil, 12 mL of DMSO and 88 mL of distilled water. In the second group of cows (GII; n = 30), 20 mL of thyme essential oil was added into 100 mL of distilled water and given intrauterine. The cows in the last group (GIII; n=30) were given intrauterine after mixing 12 mL of DMSO with 108 mL of distilled water. Rectal, ultrasonographic and vaginoscopic examination were performed 14 days after the treatment. Blood was collected from all animals immediately before and 14 days after treatment. Hp, TNF and NO levels in blood serums were determined by the ELISA commercial kits method. Hp, TNF and NO levels decreased significantly after treatment in all groups. The clinical recovery rate was found to be at the highest GI (93.3%). It was determined as 73.3% in GII and 56.6% in GIII. As a result, it was determined that DMSO administration intrauterine together with thyme essential oil treatment contributed positively to the clinical improvement of endometritis

Pnömonili sığırlarda miyeloid hücrelerde eksprese edilen mid-regional pro-adrenomedullin ve soluble tetikleyici reseptörün serum düzeyleri

This study aimed to determine the serum levels of two inflammatory biomarkers, named, mid-regional pro-adrenomedullin (MR-Pro ADM) and soluble triggering receptor expressed on myeloid cells (sTREM-1), in cattle diagnosed with pneumonia. For this purpose, 40 patient female cattle, which were aged 2-7 years and displayed coughing, dyspnea, nasal discharge, anorexia and abdominal respiration, and 15 healthy female cattle within the same age range, were evaluated. The diseased cattle underwent clinical and radiological examinations and were sampled for blood prior to receiving treatment. The healthy subjects also underwent clinical examination and were sampled for blood once. Blood samples were used for biochemical and hematological measurements. While the diseased group had higher serum levels of MR-Pro ADM (86.38±6.33), compared to the healthy control group (61.81±4.96); the pneumonic cattle had lower levels of sTREM-1 (75.93±1.86), in comparison to the healthy group (96.55±9.13). In conclusion: MR-Pro ADM and sTREM-1 levels are very important diagnostically in cattle with pneumonia.Bu çalışma, pnömoni tanısı konulan sığırlarda mid-regional pro-adrenomedullin (MR-Pro ADM) ve miyeloid hücrelerde eksprese soluble tetikleyici reseptör olmak üzere iki enflamatuvar biyobelirteç ile bazı biyokimyasal ve hematolojik parametrelerin serum düzeylerinin belirlenmesini amaçladı. Bu amaçla 2-7 yaş arası öksürük, nefes darlığı, burun akıntısı, iştahsızlık ve abdominal solunumu gösteren 40 hasta dişi sığır ve aynı yaş aralığında 15 sağlıklı dişi sığır değerlendirildi. Hasta sığırlar tedavi edilmeden önce klinik ve radyolojik muayenelere tabi tutuldu ve kan örnekleri alındı. Sağlıklı sığırlar ayrıca klinik muayeneye tabi tutuldu ve bir kez kan örnekleri alındı. Kan örnekleri biyokimyasal ve hematolojik ölçümler için kullanıldı. Hasta grupta MR-Pro ADM serum düzeyleri (86,38±6,33) kontrol grubuna (61,81±4,96) göre daha yüksek bulundu. Hasta sığırların sTREM-1 seviyeleri (75,93±1,86) sağlıklı gruba kıyasla (96,55±9,13) karşılaştırıldığında istatistiksel olarak anlamlı düşük bulundu. Sonuç olarak: MR-Pro ADM ve sTREM-1 seviyeleri pnömonili sığırlarda diagnostik açıdan oldukça önemlidir

The serum amyloid-a, haptoglobin, ceruloplasmin and albumin levels in dogs which are infected with babesia canis

Bu çalışmada Babesia canis ile doğal enfekte köpeklerde serum haptoglobin (Hp), serum amiloid-A (SAA), seruloplazmin (Cp)ve albümin (Alb) seviyelerinin belirlenmesi amaçlanmıştır. Çalışmanın materyalini, Kafkas Üniversitesi Veteriner Fakültesi İç Hastalıkları Anabilim Dalı kliniklerine getirilen, kan frotilerinin giemsa yöntemi ile boyanması sonucunda B. canis tanısı konulan 20 hasta köpek oluşturdu. Kontrol amacı ile 10 sağlıklı köpek kullanıldı. Hasta hayvanların serum Hp, SAA, Cp ve Alb seviyeleri sırasıyla 0.44±0.12 mg/mL, 43.18±13.77 µg/mL, 10.75±2.45 mg/dL, 2.74±0.15 g/dL olarak belirlendi. Sağlıklı hayvanların serum Hp, SAA, Cp ve Alb değerleri ise sırasıyla 1.84±0.29 mg/mL, 1.18±0.40 µg/mL, 4.80±0.53 mg/dL, 3.01±0.34 g/dL olarak ölçüldü. Hasta hayvanların SAA ve Cp değerleri sağlıklı hayvanlara göre daha yüksek bulunurken (P<0.001), Hp (P<0.001) ve Alb (P<0.01) seviyelerinin daha düşük olduğu belirlendi. Sonuç olarak köpeklerde B. canis enfeksiyonunda serum SAA ve Cp değerleri yükselirken, Hp ve Alb seviyelerinin düştüğü belirlendi.In this study, we aimed to determine the serum haptoglobin (Hp), serum amyloid-A (SAA), ceruloplasmin (Cp) and albümin (Alb) levels in the dogs which are naturally infected with Babesia canis. 20 patient dogs which were diagnosed as B. canis by staining of their blood smears with giemsa method, which were brought to Kafkas University Veterinary Faculty Internal Medicine Department clinics formed the material of the study. 10 Healthy dogs were used for control. The serum Hp, SAA, Cp and Alb levels of the sick animals were determined as 0.44±0.12 mg/mL, 43.18±13.77 µg/mL, 10.75±2.45 mg/dL, 2.74±0.15 g/dL, respectively. Whereas, the serum Hp, SAA, Cp and Alb values of the healthy animals were measured as 1.84±0.29 mg/mL, 1.18±0.40 µg/mL, 4.80±0.53 mg/dL, 3.01±0.34 g/dL, respectively. While the SAA and Cp values of the sick animals were determined higher than that of the healthy animals, the (P <0.001), the Hp (P <0.001) and the Alb (P <0.01) levels were found to be lower. As a result, it was determined that the serum SAA and Cp levels increased, and the Hp and Alb levels decreased in the dogs which are infected with B. canis

Tuj kuzularında farklı konsantre yemlerin bazı kan parametreleri üzerine etkisi

Bu çalışma, tuj kuzularının konsantre yemlerinde kullanılan farklı protein kaynaklarının 1, 30, 60 ve 90 günlük besi sürelerinde bazı kan metabolitleri üzerine etkisini belirlemek amacı ile yapıldı. Denemede, 3-3.5 aylık yaşta, ortalama 19.5 kg canlı ağırlıkta, 24 baş Tuj ırkı erkek kuzu kullanıldı. Rasgele seçilen ve 6 baş kuzu içeren 4 ayrı deneme grubu oluşturuldu. Her bir hayvan bireysel kafeslere konuldu. Gruplara farklı protein kaynakları içeren konsantre yem karmaları hazırlandı. Kaba yem olarak çayır kuru otu kullanıldı. Sabah yemlemesinden sonra vena jugularis’ten 1, 30, 60 ve 90. gün kan örnekleri alındı. Dört farklı bitkisel protein kaynağı içeren değişik konsantre yemlerle beslenen gruplarda 1, 30, 60, 90. günlerde alınan plazma örneklerinde yapılan analizlerde dönemlerin ve farklı protein kaynaklarının kan plazma parametreleri üzerine etkisi incelendi. Farklı protein kaynaklarıyla yapılan çalışmada bazı kan parametrelerinde farklılık saptansa da parametrelerin düzeylerinin normal sınırlarda olduğu, beslenmenin bazı kan parametreleri üzerine etkisi olabileceği saptandı. Bu araştırma sonucunda, rasyonlarda protein kaynağı olarak mısır gluten unu, pamuk tohumu küspesi, soya küspesi ya da ayçiçeği küspesi kullanılmasının bazı kan parametreleri üzerine olumsuz etkisinin olmadığı kanısına varıldı.This study was performed to determine the effects of various protein sources on certain blood metabolites, added to the Tuj lamb concentrates used for 1, 30, 60, and 90 day periods. In the study, a number of 24 weaned Tuj lambs, male 3-3.5 months old and weighing meanly 19.5 kg, were randomly divided into four groups, and each animal was put in individual cages. Concentrates containing various protein sources were prepared, and hay was used as roughage. Blood samples were obtained through the jugular vein on the days of 1, 30, 60, and 90 after the morning feeding. The effects of the periods and various protein sources were determined using the plasma samples obtained on the first, 30th, 60th, and 90th days from the groups fed with the concentrates possessing four different phyto-protein sources. The parameters obtained hereby were at normal levels even though there were numerical differences in some of the blood parameters, thus nourishment may have certain effects on these parameters. Overall, this study has revealed that using corn gluten meal, cotton seed meal, soybean meal, and sunflower meal as protein sources has no negative effect on certain blood parameters

The effects of different monochromatic colors on oxidant antioxidant balance in broilers

Poultry management is associated with light due to positive effects of photoreceptors on animal’s growth, immunity and homeostasis. Colors influence the efficiency of poultry performance and behavior. Light-emitting diode (LED) can use for providing monochromatic light source. In this trial, two hundred Ross 308 broiler chicks were studied in four different light groups as follows; White (Control), Red (RL), green (GL) and blue (BL) during 42 trial days. At the end of the study, the serum Superoxide dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GPx) and malondialdehyde (MDA) enzyme activities were measured to evaluate the oxidant-antioxidant balance. The oxidant marker MDA was found the highest value in group RL (p:0.0001 ; 9.24±0.33). On the other hand, in group GL, all antioxidant parameters were found the highest value (p<0.05). Also, SOD and GPx values were increased in group GL (SOD: 150.61±9.14 ; GPx: 186.96±10.50) than BL (SOD: 139.29±5.19; GPx: 160.02±3.15) statistically (p<0.05). The results showed that red light may be a stressor, and on the contrary, green light may be a positive monochromatic color for growth, oxidant-antioxidant balance and homeostasis for broilers

Evaluation of acute phase response in cattle with naturally infected bovine ephemeral fever virus

Bovine ephemeral fever is an arthropod-borne viral disease that primarily affects cattle and causes economic losses mainly due to the sudden decrease in milk yield. We aimed to reveal the biochemical reflection of the acute phase response by detecting the changes in serum acute phase proteins in cattle naturally infected with the bovine ephemeral fever virus. The material of this study consisted of 21 Simmental dairy cows (4–6 years old) naturally infected with bovine ephemeral fever virus (infected group) and 10 clinically and serologically healthy Simmental dairy cows (control group). The prevalence of the disease in suspected cattle was 52 per 100. It was determined that in infected cows levels of serum haptoglobin, serum amyloid A (P ˂ 0.001), and ceruloplasmin increased significantly (P ˂ 0.05), whereas levels of albumin decreased significantly (P ˂ 0.05). It is thought that the acute phase proteins variation pattern for bovine ephemeral fever would be beneficial both in better understanding the pathogenesis of the disease and in determining the diagnosis and prognosis correctly

Protective Effects of Betanin against Oxidative Stress in a Peripheral Artery Vasospasm Model in Rat

Objective: The aim of this study is to determine protective/modulatory effects of betanin in a femoral artery vasospasm model in rats. Materials and Methods: Sprague-Dawley rats were divided into three groups. Group 1: sham (n = 7), group 2: vasospasm model only (n = 7), group 3: postoperative betanin treatment in the vasospasm model (n = 7). 100 mg/kg betanin was administered orally to group 3 for 7 days, postoperatively. Peripheral blood malondialdehyde (MDA) and nitric oxide (NO) levels were measured for the quantification of oxidative stress, lumen diameter and wall thickness of femoral artery segments were determined to assess vasodilator effects of betanin. Results: Femoral artery vasospasm formation significantly increased both MDA (13.54 ± 3.09 mmol/mL) and NO levels (0.61 ± 0.06 µmol/mL) relative to the sham (9.07 ± 1.09 and 0.48 ± 0.1, respectively). Upon betanin administration, both MDA and NO approached baseline levels (9.95 ± 0.92 and 0.5 ± 0.06, respectively). Pathological examination of lumen diameter and wall thickness of the femoral arteries also revealed that betanin administration resulted in significant increase in lumen diameter when compared to vasospasm group (614.15 ± 245.77 versus 117.40 ± 46.19 µm) and decrease in wall thickness (64.68 ± 14.13 versus 96.73 ± 9.20 µm). Conclusion: Betanin was shown to have protective effect against oxidative stress in a peripheral artery vasospasm model in rats. It may also have a role in mitigating maladaptive changes in arterial structure, as shown in pathological examination

Serum paraoxonase activity and total sialic acid in sheep with foot and mouth disease

DEVECI, HACI AHMET/0000-0002-5613-0520;WOS: 000427048800011The aim of this study was to investigate paraoxonase (PON1) activity and total sialic acid to be measured for the first time in sheep infected naturally with foot and mouth disease, and their relationships with oxidative stress. A total of 30 Awassi sheep (aged between 2-4), which were healthy and infected with the foot and mouth disease virus (FMDV) were used in the study. Concentrations of paraoxonase activity (PON1), which is an important antioxidant against oxidative stress, high density lipoprotein (HDL) and total sialic acid (TSA), which has a critical role for immune system and is one of the significant indicators of cellular degeneration, were measured in serum samples drawn from animals. The total oxidant capacity (TOC) and total antioxidant capacity (TAC) were evaluated to determine the oxidative balance. It was observed that serum PON1 (P < 0.001) and HDL (P < 0.01) concentrations were significantly lower compared to the control group. TSA concentration was higher in the infected group (P < 0.001) compared to the healthy group. TOC was higher (P < 0.001) and TAC was lower (P < 0.01) in the infected group compared to the control group. Consequently, harmful effects of the infection of foot and mouth disease were shown as cause of oxidative cell damage and the high rate of sialic acid was produced depending on the degeneration during the infection