The Hard Look Doctrine: How Disparate Impact Theory Can Inform Agencies on Proper Implementation of NEPA Regulations

Executive Order 12898—Federal Actions to Address Environmental Justice in Minority Populations and Low-Income Populations—was issued to achieve “environmental protection for all communities” by drawing federal agencies’ attention to the environmental and human health effects brought about by their actions. The National Environmental Policy Act (NEPA) sets forth a detailed process which aims to ensure that each agency will have available, and will consider, a carefully detailed compilation of information concerning significant environmental impacts resulting from federal actions before taking those actions. Realizing the Executive Order’s goal, however, is rendered problematic, in part because of the difficulty in challenging an Environmental Impact Statement (EIS) issued pursuant to NEPA and the subsequent federal action. When preparing an EIS, an agency is “not required to select the course of action that best serves environmental justice, [but is] only [required] to take a ‘hard look’ at environmental justice issues.” The hard look doctrine is a principle of administrative law whereby courts must examine the methodology and substance of agency decisions to ensure that they have adequate factual support. However, it is currently unclear how much data is enough for a NEPA analysis; when an agency should collect more current, or arguably more representative, information; or how an agency should choose and apply appropriate methods and models for evaluating environmental effects. This Note proposes that, to ensure environmental justice, a disparate impact analysis must be used by the courts instead of a hard look test when either creating or evaluating an EIS to clarify four key factors: (1) the data sufficiency; (2) data gaps; (3) stale data; and (4) the scientific integrity of models and methodologies

What Your Heart Doth Know

Combining embryological insight with careful analysis of early stage cardiomyocyte differentiation, Kattman et al. (2011) in this issue of Cell Stem Cell have defined minimal culture conditions to efficiently produce cardiomyocytes from hESCs and hiPSCs. The lessons learned are applicable to the derivation of other organotypic cell types

Integrated Communication: Perceptions among the Fields of Marketing, Public Relations, and Journalism

The literature indicates the importance of the public relations, marketing, and journalism disciplines being aware of the professional demands of each area. An analysis of student perceptions from each discipline about the other disciplines reveals the need to do more of an integrated approach in the teaching domain. A research instrument surveyed classes in public relations/journalism and marketing/management in the College of Business and the Communication Department

Developing microRNA screening as a functional genomics tool for disease research

Originally discovered as regulators of developmental timing in C. elegans, microRNAs (miRNAs) have emerged as modulators of nearly every cellular process, from normal development to pathogenesis. With the advent of whole genome libraries of miRNA mimics suitable for high throughput screening, it is possible to comprehensively evaluate the function of each member of the miRNAome in cell-based assays. Since the relatively few microRNAs in the genome are thought to directly regulate a large portion of the proteome, miRNAome screening, coupled with the identification of the regulated proteins, might be a powerful new approach to gaining insight into complex biological processes

THE TRANSCRIPTIONAL FACTOR EGR-1 IS SYNTHESIZED BY BACULOVIRUS-INFECTED INSECT CELLS IN AN ACTIVE, DNA-BINDING FORM

The Egr-1 (zfp-6) gene encodes a zinc-finger-containing nuclear protein that is rapidly and transiently induced in quiescent cells treated with mitogens. We have constructed baculovirus vectors that synthesize mouse Egr-1 protein initiating at two putative ATG start sites. The ATG site producing the larger protein (M(r), 80,000) is similar, if not identical, to Egr-1 synthesized by serum-stimulated quiescent mouse fibroblasts, thus identifying the likely site for translation. The protein synthesized by the insect cells is active as assayed by its ability to bind to a specific DNA sequence that has been identified as an Egr-1 binding site. The insect cell system will allow further studies of the structure and function of the Egr-1 product, a protein that appears to be an important "master switch" for other genes

The identification of trans-associations between prostate cancer GWAS SNPs and RNA expression differences in tumor-adjacent stroma

Here we tested the hypothesis that SNPs associated with prostate cancer risk, might differentially affect RNA expression in prostate cancer stroma. The most significant 35 SNP loci were selected from Genome Wide Association (GWA) studies of ~40,000 patients. We also selected 4030 transcripts previously associated with prostate cancer diagnosis and prognosis. eQTL analysis was carried out by a modified BAYES method to analyze the associations between the risk variants and expressed transcripts jointly in a single model. We observed 47 significant associations between eight risk variants and the expression patterns of 46 genes. This is the first study to identify associations between multiple SNPs and multiple in trans gene expression differences in cancer stroma. Potentially, a combination of SNPs and associated expression differences in prostate stroma may increase the power of risk assessment for individuals, and for cancer progression

Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential

Proper maintenance of stem cells is essential for successful utilization of ESCs/iPSCs as tools in developmental and drug discovery studies and in regenerative medicine. Standardization is critical for all future applications of stem cells and necessary to fully understand their potential. This study reports a novel approach for the efficient, consistent expansion of human ESCs and iPSCs using laser sectioning, instead of mechanical devices or enzymes, to divide cultures into defined size clumps for propagation. Laser-mediated propagation maintained the pluripotency, quality, and genetic stability of ESCs/iPSCs and led to enhanced differentiation potential. This approach removes the variability associated with ESC/iPSC propagation, significantly reduces the expertise, labor, and time associated with manual passaging techniques and provides the basis for scalable delivery of standardized ESC/iPSC lines. Adoption of standardized protocols would allow researchers to understand the role of genetics, environment, and/or procedural effects on stem cells and would ensure reproducible production of stem cell cultures for use in clinical/therapeutic applications

Egr1 regulates the coordinated expression of numerous EGF receptor target genes as identified by ChIP-on-chip

UV stimulation of prostate cells causes an apoptotic response that is dependent on the zinc finger transcription factor Egr1; downstream targets of Egr1 in this response were identified