4,559 research outputs found
Defining the Role for ZBP-89 in ATM-mediated DNA Damage Response to Irradiation in the Intestine
ZBP-89 (ZNF148, Zfp148) is a zinc-finger transcription factor that inhibits cellular proliferation when overexpressed in cell lines. ZBP-89 forms a protein-protein interaction with p53 and Ataxia-telangiectasia mutated (ATM). However, it is unclear how the interaction modulates the function of these two proteins in vivo.
Double strand DNA breakage induced by -irradiation induces ATM phosphoinositol kinase activity, which initiates a cascade of events culminating in cell cycle arrest, DNA repair or apoptosis. Elevated levels of ZBP-89 induce growth arrest and apoptosis in gastrointestinal cell lines. Therefore, we hypothesize that ZBP-89 facilitates cell growth arrest and activation of the DNA repair pathway after ATM activation.
To test this hypothesis, we irradiated 4 groups of mice—1) C57BL/6 mice without any genetic deletion; 2) mice with one or both copies of ATM deleted from the intestinal mucosa; 3) mice with the ATM deletion and one copy of Zfp148 deleted; 4) ATM deletion with both copies of Zfp148 deleted. After the intestines were fixed, paraffin-embedded and sectioned, I performed the de-paraffinization step for immunohistochemistry of ZBP-89, p53 and H2AX. To stain for ZBP-89 protein, I performed an antigen retrieval step using sodium citrate, followed by blocking with 3% hydrogen peroxide and serum. I then added the primary antibody then the secondary antibodies. I used diaminobenzidine (DAB), a chromogenic detection method to visualize the antigen-antibody complex. I then counterstained with hematoxylin.
We predict that the mice with a deletion of ZBP-89 will exhibit more tissue damage as a result of the irradiation and DNA damage. Results are pending
Imports versus Domestic Production: A Demand System Analysis of the U.S. Red Wine Market
This research estimates price and expenditure elasticities of U.S. red wine imports from five countries--Italy, France, Spain, Australia, and Chile--which are compared to elasticities of domestically produced red wine using the first-difference version of the almost ideal demand system (AIDS). Expenditure elasticity results indicate that if U.S. total expenditures on red wine increase, domestic producers would gain most. Empirical results for conditional own-price elasticities of demand indicate that U.S. and Chilean red wines are elastic while U.S. demand for red wines from other countries are highly inelastic. Due to the magnitude of consumption of U.S. domestic red wines relative to imports, an increase in the price of U.S. wine results in a decline in quantity demanded that is six times larger than that for French and Italian red wines and over 20 times larger than that of other import countries. Results suggest that U.S. red-wine producers could increase their total revenue by decreasing prices, while Italian and French producers can increase total revenues by increasing prices.imports, red wines, Almost Ideal Demand System, AIDS, Demand and Price Analysis, International Relations/Trade,
Effects of Diffusion on Photocurrent Generation in Single-Walled Carbon Nanotube Films
We have studied photocurrent generation in large carbon nanotube (CNT) films
using electrodes with different spacings. We observe that the photocurrent
depends strongly on the position of illumination, with maximum observed
response occurring upon illumination at the electrode edges. The rate of change
of the response decays exponentially, with the fastest response occurring for
samples with the smallest electrode spacing. We show that the time response is
due to charge carrier diffusion in low-mobility CNT films
Neoplatonism and the Florentine Renaissance
This thesis demonstrates the correspondence between the visual arts and the literary sources of a given period in art history. During the Florentine Renaissance this correspondence lay between the Neoplatonism of Marsilio Picino and Giovanni Pico della Mirandola and the Visual art of the predominant artists; specifically, Sandro Botticelli and Michelangelo Buonarrotti. The impulse that is common to these creative minds is the Neoplatonic conception or the visual image. It is through a study or this tacit dimension that we are able to some extent to view the meaning of Renaissance art
A role for CITED2, a CBP/p300 interacting protein, in colon cancer cell invasion
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/116311/1/feb2s0014579307012264.pd
B493: Consumer Acceptance of Washed Maine Potatoes
The broad objective of this study was to determine the feasibility as well as the problems associated with washing Maine potatoes. This is particularly important at this time in view of the general preference of housewives for clean potatoes as determined by a national survey by the United States Department of Agriculture and the increasing supplies of washed potatoes from competing areas in markets Maine normally serves. Specific objectives of this study were to determine (1) the keeping qualities of washed potatoes, (2) the costs associated with washing Maine potatoes including equipment, fuel, water, and shrinkage, and (3) the acceptance of washed Maine potatoes by the wholesale and retail trade.https://digitalcommons.library.umaine.edu/aes_bulletin/1079/thumbnail.jp
\u3ci\u3eEragrostis Curvula\u3c/i\u3e Effects on Above and Below-Ground Plant Species Richness and Diversity
Monitoring and managing the soil seed bank is fundamental to land management as it constitutes the future generations of invasive plant communities. Invasive plants have traits that result in high recruitment through increased seed generation, short seed dormancy and phenotypic plasticity. Furthermore, invasive plants with growth forms that inhibit the growth and recruitment of other species can lead to monocultures and associated reduction in above-ground biodiversity, potentially negatively impacting the soil seed bank diversity and ecosystem functions and services. Eragrostis curvula is one such species that has many of these invasive traits, including high propagule generation, and can exclude plant species from establishing in the above-ground population, thus negatively impacting above-ground biodiversity, as measured by species richness and Shannon diversity index. However, our findings suggest it has not significantly impacted the soil seed bank species diversity or richness across eight sites within the Snowy Monaro region when competition is removed as a limiting factor. Our findings provide valuable information on a path to invasive plant species management. If E. curvula above-ground biomass is controlled, other species dormant in the soil seed bank may recruit in the ecosystem, provided they remain viable in the soil seed bank. However, to date, our research has not investigated the species composition of these sites in detail. With such a high density of potentially germinating seeds in a soil seed bank and the adverse effects the species can have on the above-ground species diversity and richness, E. curvula needs integrated management to mitigate its spread and ecosystem and economic impact
Laying the Groundwork for a National Impact Investing Marketplace
The practice of impact investing is rapidly gaining momentum, but the level of activity among individual and institutional investors, including philanthropists and foundations, has barely penetrated projections of market potential.
The marketplace that should connect impact investors with investees or social ventures does not function effectively.
Developing cost-effective ways to engage new investors and break down barriers to investment is an essential part of growing the industry.
Developing cost-effective ways to “prime the pump” for social ventures to become investor-ready — through a capacity-building process that includes outreach, education, and technical assistance — is an essential part of growing the industry.
The Impact Finance Center partnered with foundations and other investors in Colorado to create “CO Impact Days and Initiative” to demonstrate how to address this need for a more efficient and effective marketplace.
CO Impact Days and Initiative was designed to expand regionally and be replicated
Influence of spin waves on transport through a quantum-dot spin valve
We study the influence of spin waves on transport through a single-level
quantum dot weakly coupled to ferromagnetic electrodes with noncollinear
magnetizations. Side peaks appear in the differential conductance due to
emission and absorption of spin waves. We, furthermore, investigate the
nonequilibrium magnon distributions generated in the source and drain lead. In
addition, we show how magnon-assisted tunneling can generate a fullly
spin-polarized current without an applied transport voltage. We discuss the
influence of spin waves on the current noise. Finally, we show how the magnonic
contributions to the exchange field can be detected in the finite-frequency
Fano factor.Comment: published version, 15 pages, 10 figure
- …